Table S3: Recombination data set
(A) The localisation of the six FTL intervals used in this study are shown below on the five
Arabidopsis chromosomes. They correspond to three pairs of linked intervals I5a I5b, I5c I5d
and I3b I3c described by Berchowitz and Copenhaver (Berchowitz & Copenhaver, 2008). The
position (in bp) of each transgene encoding red, yellow or cyan fluorescent proteins (filled
circles) is shown. The size of each interval is given in the accompanying table. The map was
obtained using the Chromosome Map Tool from
http://www.arabidopsis.org/jsp/ChromosomeMap/tool.jsp
498 916
3 126 994
4 319 513
I 3b
I 3c
2 372 623
I 5c
3 760 756
5 497 513
I 5d
Intervals
I5a
I5b
I5c
I5d
I3b
I3c
size (bp)
4 916 298
2 650 744
1 388 133
1 736 757
2 628 078
1 192 519
18 164 269
I 5a
23 080 567
I 5b
25 731 311
(B) To measure recombination rates, we produced plants homozygous for the quartet (qrt)
mutation, heterozygous for axr1 (N877898 allele) and carrying or not three linked
fluorescent markers (R red, Y yellow, C Cyan): qrt-/- axr1+/- and qrt-/- axr1+/- RYC/RYC. The qrt
mutation allows the four pollen grains from a single meiosis to be maintained together. We
crossed these two plants and in the progeny analysed tetrad fluorescence of mutant plants
(qrt-/- axr1-/- RYC/+++) or fertile plants (wt) (either qrt-/- axr1+/- RYC/+++ or qrt-/- axr1+/+
RYC/+++). Plants were grown in a greenhouse and tetrad analyses were carried out as
described by Berchowitz and colleagues in (Berchowitz & Copenhaver, 2008). The
distribution of the markers within the tetrads and the resulting distribution of colours vary
depending on the number, localisation and chromatids involved in recombination. The
different possibilities are indicated by drawings and the number of tetrads in each
phenotypic class is shown in the tables below. Two replicates were made for the I5a I5b
intervals.