1.877.777.4004 info@paternitycanada.com F-2020 Corydon Ave. Winnipeg, MB, Canada R3P 0N2 Understanding My DNA Report Your DNA report presents the genetic profile for each tested party and two (2) important values used to determine if the tested parties are related. These values are the Combined Relationship Index and the Probability of Relationship. Definition of Terms Locus: A specific region of the DNA molecule (ex. D3S1358) Allele: A genetic variation found at a specific locus. In the DNA report, alleles are represented by numbers (ex. 16-17). For each locus, one allele comes from the mother and one allele comes from the father. Index: This value represents the strength of the genetic evidence based on the frequency of each allele in the population. It indicates whether the genetic evidence is more in agreement with the tested parties being related or more in agreement with the tested parties not being related. Probability: A way to express the strength of a biological relationship. It is based on the genetic evidence (combined index) and non-genetic evidence (prior probability). Since the laboratory does not possess information on non-genetic evidence, the prior probability is fixed at 50%. DNA Analysis Example of a POSITIVE paternity test Locus D3S1358 FGA D21S11 D13S317 1. 2. 3. 4. 5. 6. Mother 16 – 17 22 – 27 34 – 35 12 – 13 Child 15 – 16 22 – 23 27 – 35 10 – 12 Alleged Father 15 – 17 21 – 23 27 – 28 10 – 14 Combined Paternity Index Probability of Paternity Paternity Index 1.79 2.64 8.47 21.65 866 99.88% Identify the allele that the mother gave the child (red) at each locus Check if the tested alleged father has the child’s other allele (blue) If there is a match, the paternity index is calculated for each locus The index values are then multiplied to give the combined paternity index The combined paternity index is converted to the probability of paternity A probability of paternity >= 99.8% confirms the paternity of the tested father Example of a Negative paternity test Locus Mother Child Alleged Father Paternity Index D3S1358 16 – 17 15 – 16 12 – 19 Exclusion FGA 22 – 27 22 – 23 19 – 23 D21S11 34 – 35 27 – 35 27 – 28 Exclusion 1. Identify the allele that the mother gave the child (red) at each locus 2. Check if the tested alleged father has the child’s other allele (blue) 3. If there is no match for at least 2 loci, the tested alleged father cannot be the father of the child since he does not possess the alleles that the child received from his/her biological father 4. The probability of paternity is 0% Please contact us if you require more information. We would be happy to assist you in answering your questions. Ph. 1.877.777.4004 • Em. info@paternitycanada.com • Addr. F-2020 Corydon Ave. Winnipeg, MB, Canada, R3P 0N2