Defining the recombination landscape of Arabidopsis
Gregory Paul Copenhaver
Associate Professor
Department of Biology
The University of North Carolina at Chapel Hill
Campus Box 3280
Chapel Hill, North Carolina 27599
gcopenhaver@bio.unc.edu
http://www.bio.unc.edu/faculty/copenhaver/default.htm
PLoS Genetics – Deputy Editor-in-Chief
Genetics – Associate Editor
Undergraduate Study
University of California Riverside, Riverside, California. B.S. in Botany (high distinction) 1990.
Graduate Study
Washington University, St. Louis, Missouri. Ph.D. in Biology and Biomedical Sciences, 1996.
Post-Graduate Study
University of Chicago, Chicago, Illinois. Postdoctoral research with Dr. Daphne Preuss. 2001.
Professional Experience
Associate Professor, UNC, Chapel Hill, North Carolina, Dept. of Biology. 2008 – present
Director of Graduate Studies (MCDB), UNC, Chapel Hill, North Carolina, Dept. of Biology. 2008 present
Assistant Professor, UNC, Chapel Hill, North Carolina, Dept. of Biology. 2001 - 2007
Publications:
1. Wang Y, Cheng Z, Huang J, Shi Q, Hong Y, Copenhaver GP, Gong Z, Ma H (2012) The DNA
Replication Factor RFC1 is Required for Interference-Sensitive Meiotic Crossovers in
Arabidopsis thaliana. PLoS Genetics 8(11): e1003039. doi:10.1371/journal.pgen.1003039
2. De Storme N, Copenhaver GP, Geelen D (2012) Production of diploid male gametes in
Arabidopsis by cold-induced destabilization of post-meiotic radial microtubule arrays. Plant
Physiology, 160(4): 1808-1826. doi: 10.1104/pp.112.208611
3. Sun Y, Ambrose JH, Haughey BS, Webster TD, Pierrie SN, Muñoz DF, Wellman EC, Cherian S,
Lewis SM, Berchowitz LE and Copenhaver GP (2012) Deep Genome-wide measurement of
meiotic gene conversion using tetrad analysis in Arabidopsis thaliana. PLoS Genetics 8(10):
e1002968. doi:10.1371/journal.pgen.1002968.*
4. Yelina NE, Choi K, Chelysheva L, Macaulay M, de Snoo B, Wijnker E, Miller N, Drouaud J,
Grelon M, Copenhaver GP, Mezard C, Kelly KA and Henderson IR (2012) Epigenetic
Remodeling of Meiotic Crossover Frequency in Arabidopsis thaliana DNA Methyltransferase
Mutants. PLoS Genetics 8(8): e1002844. doi:10.1371/journal.pgen.1002844
5. Barsh GS, Copenhaver GP, Gibson G, Williams SM (2012) Guidelines for genome-wide
association studies. PLoS Genetics 8(7) e1002812.doi:10.1371/journal.pgen.1002812.
6. Crismani W, Girard C, Forger N, Pradillo M, Santos JL, Chelyscheva L, Copenhaver GP, Horlow
C and Mercier R (2012) FANCM Limits Meiotic Crossovers. Science 336: 1588-1590.*
7. Luo S, Mach JM, Abramson BW, Ramirez R, Schurr R, Barone P, Copenhaver GP and Folkerts O
(2012) The Cotton Centromere Contains a Ty3-gypsy-like LTR Retroelement. PLoS ONE 7(4):
e35261. doi:10.1371/journal.pone.0035261
8. Roy B, Copenhaver GP, von Arnim AG (2011) Fluorescence-Tagged Transgenic Lines Reveal
Genetic Defects in Pollen Growth – Application to the Eif3 Complex. PLoS ONE 6(3): e17640.
doi:10.1371/journal.pone.0017640.
9. Gibson G, Copenhaver GP (2010) Consent and Internet-Enabled Human Genomics. PLoS
Genetics 6(6): e1000965. doi:10.1371/journal.pgen.1000965.
10. d'Erfurth I, Cromer L, Jolivet S, Girard C, Horlow C, Sun Y, To JPC, Berchowitz LE, Copenhaver
GP, Mercier R (2010) The CYCLIN-A CYCA1;2/TAM Is Required for the Meiosis I to Meiosis II
Transition and Cooperates with OSD1 for the Prophase to First Meiotic Division Transition.
PLoS Genetics 6(6): e1000989. doi:10.1371/journal.pgen.1000989.
11. Copenhaver GP & Preuss D (2010) Haploidy with histones. Nature Biotechnology 28(5):
423-424.
12. Berchowitz LE, Copenhaver GP (2010) Genetic interference: don’t stand so close to me.
Current Genomics 11:91-102.
13. Berchowitz LE, Hanlon SE, Lieb JD, Copenhaver GP (2009) A positive but complex association
between meiotic double-strand break hotspots and open chromatin in Saccharomyces
cerevisiae. Genome Research 19:2245-2257*.
14. Barsh GS, Copenhaver GP (2009) Scientists ← Editors ← Scientists: Past, present, and
future for PLoS Genetics. PLoS Genet 5(7): e1000580.
15. Berchowitz LE and Copenhaver GP (2009) Visual Markers for Detecting Gene Conversion
Directly in the Gametes of Arabidopsis thaliana. In Meiosis Volume 1 Molecular and
Genetic Methods, ed. Scott Keeney Volume 557 of Methods in Molecular Biology, Humana
Press.
16. Berchowitz LE and Copenhaver GP (2008) Division of Labor Among Meiotic Genes. Nature
Genetics 40:266-267.
17. Berchowitz LE, Copenhaver GP (2008) Fluorescent Arabidopsis tetrads: A visual assay for
quickly developing large crossover and crossover interference datasets. Nature Protocols
3(1): 41-50*.
18. Copenhaver GP and Hooykaas PJJ (2007) Plant DNA Repair and Recombination on the French
Mediterranean. EMBO Reports 8:1115-9.
19. Carlson SR, Rudgers GW, Zieler H, Mach JM, Luo S, Grunden E, Krol C, Copenhaver GP, Preuss
D (2007) Meiotic transmission of an in vitro-assembled autonomous maize mini-chromosome.
PLoS Genetics 3(10): e179 doi:10.1371/journal.pgen.0030179.
20. Berchowitz LE, Francis KE, Bey AL, Copenhaver GP (2007) The Role of AtMUS81 in
Interference-Insensitive Crossovers in A. thaliana. PLoS Genetics 3: e132.
21. Sandaklie-Nikolova L, Palanivelu R, King E, Copenhaver GP, Drews GN (2007) Synergid Cell
Death in Arabidopsis is Triggered Following Direct Interaction with the Pollen Tube. Plant
Physiology 144: 1753-1762.
22. Francis KE, Lam SY, Harrison BD, Bey AL, Berchowitz LE and Copenhaver GP (2007) Pollen
Tetrad-based Visual Assay for Meiotic Recombination in Arabidopsis. Proc. Natl. Acad.
Sci.104: 3913-3918*.
23. Francis KE, Berchowitz LE, Copenhaver GP (2006) Separation of Arabidopsis Pollen Tetrads is
Regulated by QRT1, a Pectin Methylesterase Gene. Plant Physiology 142: 1004-1013.
24. Copenhaver GP (2006) A Meiotic Tapas Menu. PLoS Genetics 2(2): 100-103.
25. Lam S, Horn SR, Radford SJ, Housworth EA, Stahl FW and Copenhaver GP (2005) Crossover
interference on the NOR bearing chromosomes of Arabidopsis. Genetics 170: 807-812.
26. Copenhaver GP (2005) Plant Genetics: When Not to Interfere. Current Biology 15(8): R1-2.
27. Copenhaver GP (2005) A Compendium of Plant Species Producing Pollen Tetrads. J. N.
Carolina Acad. of Sci. 121(1): 17-35.
28. Copenhaver GP (2004) Who's driving the centromere? Journal of Biology 3:17.
29. Stahl FW, Foss HM, Young LS, Borts RH, Abdullah MFF and Copenhaver GP (2004) Does
Crossover Interference Count in Saccharomyces cerevisiae? Genetics 168: 35-48*.
30. Hall A, Hall S, Keith K, Copenhaver GP, Preusss D (2004) The rapidly evolving field of plant
centromere. Curr. Op. Plant Biol. 7: 104-1-7.
31. Copenhaver GP (2003) Using Arabidopsis to understand centromere function: Progress and
prospects. Chromosome Research 11: 255-262.
32. Chang Y-L, Henriquez X, Copenhaver GP, Preuss D, and Zhang H-B (2003) A Plant
Transformation-competent Binary BAC (BIBAC) library from the Landsberg ecotype of
Arabidopsis thaliana. Theoretical and Applied Genetics 106: 269-276.
33. Krysan PJ,. Young JC,. Jester PJ, Monson S, Copenhaver G, Preuss D, and Sussman MR (2002).
Characterization of T-DNA Insertion Sites in Arabidopsis thaliana and the Implications for
Saturation Mutagenesis. Omics 6: 163-175.
34. Copenhaver GP, Housworth E, Stahl FS (2002) Crossover Interference in Arabidopsis.
Genetics 160: 1631-1639.
35. The Arabidopsis Genome Initiative: Genome Sequencing Groups: Kaul S, Koo HL, Jenkins J,
Rizzo M, Rooney T, Tallon LJ, Feldblyum T, Nierman W, Benito M-I, Lin X, Town CD, Venter JC,
Fraser CM, Tabata S, Nakamura Y, Kaneko T, Sato S, Asamizu E, Kato T, Kotani H, Sasamoto S,
Ecker JR, Theologis A, Federspiel NA, Palm CJ, Osborne BI, Shinn P, Conway AB, Vysotskaia VS,
Dewar K, Conn L, Lenz CA, Kim CJ, Hansen NF, Liu SX, Buehler E, Altafi H, Sakano H, Dunn P,
Lam B, Pham PK, Chao Q, Nguyen M, Yu G, Chen H, Southwick A, Lee JM, Miranda M, Toriumi
MJ, Davis RW, Wambutt R, Murphy G, Düsterhöft A, Stiekema W, Pohl T, Entian K-D, Terryn N,
Volckaert G, Salanoubat M, Choisne N, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G,
Artiguenave F, Weissenbach J, Quetier F, Wilson R, de la Bastide M, Sekhon M, Huang E,
Spiegel L, Gnoj L, Pepin K, Murray J, Johnson D, Habermann K, Dedhia N, Parnell L, Preston R,
Hillier L, Chen E, Marra M, Martienssen R, McCombie WR, Genome Analysis Group: Mayer
K, White O, Bevan M, Lemcke K, Creasey TH, Bielke C, Haas B, Haase D, Maiti R, Rudd S,
Peterson J, Schoof H, Frishman D, Morgenstern B, Zaccaria P, Ermolaeva M, Pertea M,
Quackenbush J, Volfovsky N, Wu D, Lowe T, Salzberg SL, Mewes H-W, Contributing Authors:
Rounsley S, Bush D, Subramaniam S, Levin I, Norris S, Schmidt R, Acarkan A, Bancroft I,
Quetier F, Brennicke A, Eisen JA, Bureau T, Legault B-A, Le Q-H, Agrawal N, Yu Z, Martienssen
R, Copenhaver GP, Luo S, Pikaard CS, Preuss D, Paulsen IT, Sussman M, Britt A, Selinger DA,
Pandey R, Mount DW, Chandler VL, Jorgensen RA, Juergens G, Meyerowitz EM, Ecker JR,
36.
37.
38.
39.
40.
41.
42.
43.
44.
45.
46.
Theologis A, Dangl J & Jones JDG, Chen M, Chory J, and Somerville C.(2000) Analysis of the
genome sequence of the flowering plant Arabidopsis thaliana. Nature 408: 796-815*.
Copenhaver GP, Keith KC, Preuss D (2000) Tetrad Analysis in Higher Plants: A Budding
Technology. Plant Physiology 124:7-15*.
Copenhaver GP, Nickel K, Kuromori T., Benito M-I, Kaul S, Lin X, Bevan M, Murphy G, Harris B,
Parnell LD, McCombie WR, Martienssen RA, Marra M, Preuss D (1999) Genetic Definition and
Sequence Analysis of Arabidopsis Centromeres. Science 286: 2468-74.
Lin X, Kaul S, Rounsley S, Shea TP, Benito M-I, Town CD, Fujii CY, Mason T, Bowman CL,
Barnstead M, Feldblyum T, Buell CR, Ketchum KA, Ronning CM, Koo H, Moffat K, Cronin L,
Shen M, Pai G, Van Aken S, Umayam L, Tallon L, Gill J, Adams MD, Carrera AJ, Creasy TH,
Goodman, HM, Somerville CR, Copenhaver GP, Preuss D., Nierman WC, White O, Eisen JA,
Salzberg S, Fraser, CM, Venter JC (1999) Sequence and Analysis of Chromosome 2 of
Arabidopsis thaliana. Nature 402: 761-68*
Copenhaver GP, Preuss D. (1999) Centromeres in the genomic era: Unraveling paradoxes.
Curr. Op. Plant Biol. 2: 104-108.
Copenhaver GP, Browne WE, Preuss D (1998) Assaying genome-wide recombination and
centromere functions with Arabidopsis tetrads. Proc. Natl. Acad. Sci 95:247-252.
Copenhaver GP, Pikaard CS (1996) Pulsed-field gel electrophoresis of Arabidopsis thaliana
chromosomal DNA. Weeds World 3(i): 17-2
Copenhaver GP, Pikaard CS (1996) Two-dimensional RFLP analyses reveal megabase-sized
clusters of rRNA gene variants in Arabidopsis thaliana, suggesting local spreading of variants
as the mode for gene homogenization during concerted evolution. The Plant Journal 9(2):
273-282.
Copenhaver GP, Pikaard CS (1996) RFLP and physical mapping with an rDNA-specific
endonuclease reveals that nucleolus organizer regions of Arabidopsis thaliana adjoin the
telomeres on chromosomes 2 and 4. The Plant Journal 9(2): 259-272.
Copenhaver GP, Doelling JH, Gens SJ, Pikaard (1995) Use of RFLPs larger than 100 kbp to map
the position and internal organization of the nucleolus organizer region on chromosome 2 in
Arabidopsis thaliana. The Plant Journal 7(2): 273-286.
Putnam CD, Copenhaver GP, Denton M, Pikaard CS (1994 ) The RNA polymerase I
transactivator upstream binding factor requires its dimerization domain and
high-mobility-group (HMG) box 1 to bend, wrap and positively supercoil enhancer DNA.
Molecular and Cellular Biology 14(10): 6476-6488.
Copenhaver GP, Putnam CD, Denton M, Pikaard CS (1994) The RNA polymerase I
transcription factor UBF is a sequence-tolerant HMG-box protein that can recognize
structured nucleic acids. Nucleic Acids Research 22(13): 2651-2657.