I.
PURPOSE :
To provide a procedure to operate and maintain the Advance make UV Cabinet.
II.
SCOPE :
This procedure is applicable for the UV cabinate used for TLC testing in the
Quality
Control department
III.
RESPONSIBILITY :
It is the responsibility of all the personnel involved in the analysis using of the
equipment.
IV.
DOCUMENT REFERENCE :
SOPs :
Core procedure: Calibration of Equipment
Forms :
None
V.
OPERATION :
1.0
PRELIMINARY CHECK :
1.1
Check that the instrument and surroundings are clean, if not, clean with a soft
cloth
duster.
2.0 BASIC OPERATION :
2.1
Before using ensure the validity of calibration.
2.2
Switch on the power.
2.3
Switch on the normal light and open the front door pulling towords the front
side to
keep the TLC plates
2.4
Place the TLC plate in UV chamber horizontally on the base of the chamber.
2.5
Switch on the particular nm wave length light i.e. 254 nm or 365 nm light at
which the TLC is to be viewed .
2.6
Do not expose to UV light while handling the UV cabinet, use always normal
light
only while operations.
2.7
Switch of the power after the completion of the test.
VI. CALIBRATION :
Calibration Frequency: First week of every
month
Calibration of 254 nm lamp.
1. Weigh about 40 mg of sodium salicylate and transfer into 100 ml volumetric flask
dissolve and dilute to 100 ml with methanol.
2. Apply 5 L of the above solution on a TLC plate (glass or aluminum) with a
diameter of about 5 mm.
3. Examine the spot in UV cabinet under 254 nm keeping the plate normal to the
radiation.
4. The standard spot should be revealed without doubt i.e clearly.
Calibration of 365 nm lamp.
1. Weigh about 200 mg of sodium salicylate transfer into 100 ml volumetric flask
dissolve and dilute to 100 ml with methanol.
2. Apply 5 L of the above solution on a TLC plate (glass or aluminum) with a
diameter of about 5 mm.
3. Examine the spot in UV cabinet under 365/366 nm keeping the plate normal to the
radiation.
4. The standard spot should be revealed without doubt i.e clearly.
5. If the instrument fails in calibration, proceed as per the Core Procedure,
“Calibration of
Equipment”.
Acceptance criteria :
01. 0.2% spot should be visible at 365nm and 0.04% spot should be visible at
254nm .