ohsrm PART B School of Molecular Bioscience , Hazardous procedure: Mass spectrometry Step 3. Assess the risk Ref. # List & describe the hazards and hazardous jobs Priority 1 Solvents used as mobile phases (eg. methanol, acetonitrile) are TOXIC 3 2 Solvents used as mobile phases (eg. methanol, acetonitrile) are FLAMMABLE 3 3 3 5 Additives in mobile phases (eg. acetic, trifluoroacetic, phosphoric, formic acids) are CORROSIVE Sharps used for excising gel bands (scalpel) and for MS injections (silica needle) can cause injury High voltage associated with nanospray source could cause electrocution 6 Laser inside MALDI-TOF MS instruments could cause eye damage or blindness 5 7 Robotics components of MS machine can cause physical injury if fingers or hands etc. get in the way of the moving parts 5 4 4 4 What makes it hazardous? Consult with the workers to find out which factors are relevant: X The nature of the hazard itself X The individual(s) involved Tick relevant boxes and record observations or comments. Solvents and samples used in HPLC can pose toxic and/or flammable and/or biohazard risks. Also there is a mechanical risk due to use of needles for sample injection, and an electrical hazard. Requires specific training in use of this apparatus Not safe for use by untrained individuals Record the names of those consulted when assessing the risk Ben Crossett, Peter Kerr, Joe Dimauro, Jenny Phuyal, Zia Ahmad, Angela Nikolic, Craig Jackson Date 23/8/2010 Step 4. Control the risk(s) Describe the risk control(s) Who is responsible for implementation Due by date Wear personal protective equipment (lab coat, enclosed shoes, gloves, safety glasses/goggles when handling hazardous liquids) Individual user and their supervisor 21/4/2010 Keep ignition sources (eg. Bunsen burners) at least 3 m distant from solvents (or switched off) eg. when filling or emptying solvent reservoirs on the HPLC component of the MS machine Individual user and their supervisor 21/4/2010 Do not allow skin to come into contact with HPLC solvents and do not breathe the fumes of these solvents. Individual user and their supervisor 21/4/2010 Ensure solvent reservoirs and waste container are gas-tight, and do not allow vapours of solvents to enter the lab. HPLC waste containers should have an activated carbon filter on the exhaust port to allow airpressure equilibration but stop solvent vapours exiting the vessel. This filter should be changed at regular intervals and disposed of as hazardous waste. Alternatively, HPLC waste vessels should be located in a fume hood, or should exhaust into a fume hood. Individual user and their supervisor 21/4/2010 Keep hands well clear of injection needles and other moving parts. If using manual injection, be careful when using injection syringes – needlestick injuries can occur, which are complicated by potential injection of toxins/biohazards. Individual user and their supervisor 21/4/2010 Ensure the HPLC and MS are routinely serviced / inspected by a qualified individual at regular intervals. If any electrical or mechanical faults are suspected, stop using the machine, and get it serviced immediately. Individual user and their supervisor 21/4/2010 Do not open up the MS machine to attempt to fix it or otherwise meddle with it – this is a precision instrument, and can only be repaired by qualified technicians. Exposing the insides of the MS can damage it, and expose you to laser radiation or electric shock. Waste HPLC solvents need to be disposed of as hazardous waste. NOT DOWN THE SINK ! (Also see “disposal of hazardous chemical waste” risk assessment forms). For disposal, ensure that HPLC wastes are contained in SEALED plastic drums or bottles, that are labeled with the types of solvents present, and the relative amounts of the different solvents, before taking them to room 225 for storage and later disposal. If possible, keep different solvent types in separate waste containers. If you have been analyzing hazardous sample types (eg. pollutants, organochlorines), note the type of sample traces that will be present in the HPLC waste. Individual user and their supervisor 21/4/2010 Excess samples remaining in sample vials need to be disposed of appropriately. If biohazards exist, these need to be autoclaved or treated with bleach. (Note: do not autoclave samples that contain significant amounts of organic solvents like acetonitrile). If toxic or flammable hazards exist, these need to be disposed of appropriately in labeled containers into room 225, as above. Individual user and their supervisor 21/4/2010 Proper training of users Supervisor of researcher 21/4/2010 Development of Standard Operating Procedure (SOP) SMB Safety Commitee 21/4/2010 Reading and following the SOP Individual user and their supervisor 21/4/2010 Record the names of those consulted when deciding on risk control measures Ben Crossett, Peter Kerr, Joe Dimauro, Jenny Phuyal, Zia Ahmad, Angela Nikolic, Craig Jackson PART B completed by: Nick Coleman Date: 23/8/2010