, ohsrm PART B
Ref. # List & describe the hazards and hazardous jobs Priority
1
2
3
Solvents used as mobile phases (eg. chloroform, hexane, butanol) may be TOXIC 3
Solvents used as mobile phases (eg. hexane, butanol, ethyl acetate) may be FLAMMABLE 3
Samples being analysed may be TOXIC (eg. pollutants, metabolic inhibitors etc) 5
4
5
6
Samples being analysed may be BIOHAZARDS (derived from pathogenic microbes, clinical samples, recombinant organisms)
Reagents used for detection may be toxic (eg. ninhydrin, iodine), or corrosive (eg. sulfuric acid)
UV light hazard if UV lamp used for detection
5
4
4
What makes it hazardous?
Consult with the workers to find out which factors are relevant:
Tick relevant boxes and record observations or comments.
The nature of the hazard itself
Solvents and samples used in HPLC can pose toxic and/or flammable and/or biohazard risks. Also there is a mechanical risk due to use of needles for sample injection, and an electrical hazard.
The individual(s) involved
Requires specific training in use of this apparatus
Not safe for use by untrained individuals
Record the names of those consulted when assessing the risk Date
22/3/2010 Peter Kerr, Joe Dimauro, Jenny Phuyal, Zia Ahmad, Angela Nikolic, Robert Czolij, Craig Jackson
Describe the risk control(s) Who is responsible for implementation
Due by date
21/4/2010 Wear personal protective equipment (lab coat, enclosed shoes, gloves, safety glasses/goggles)
Keep ignition sources (eg. Bunsen burners) at least 3 m distant from
TLC apparatus and the solvents used (or switched off)
Do not allow skin to come into contact with TLC solvents and do not breathe the fumes of these solvents.
TLC apparatus should be set up in fume hood to minimize toxicity and flammability risk of solvent vapours. Apparatus should have a tightfitting lid to minimize amount of vapour that escapes
Treat the solvents used for TLC with care, and be aware of the hazards associated with them. (eg. toxic/flammable) – read the MSDS of all solvents used so you are aware of the risks. Post the MSDS in the laboratory so it can be easily accessed.
Waste TLC solvents need to be disposed of as hazardous waste. NOT
DOWN THE SINK ! (Also see “disposal of hazardous chemical waste” risk assessment forms). For disposal, ensure that TLC wastes are contained in SEALED plastic drums or bottles, that are labeled with the types of solvents present, and the relative amounts of the different solvents, before taking them to room 225 for storage and later disposal. If possible, keep different solvent types in separate waste containers. If you have been analyzing hazardous sample types, note the type of sample traces that will be present in the TLC waste.
Handle detection reagents with care, and be aware of any specific hazards associated with these – eg. ninhydrin and iodine are toxic, sulfuric acid is corrosive. Be especially wary when spraying these reagents – wear safety glasses/goggles! Dispose of excess detection reagents as hazardous waste (above). Read the MSDS!
Individual researcher and their supervisor
Individual researcher and their supervisor
Individual researcher and their supervisor
Individual researcher and their supervisor
Individual researcher and their supervisor
Individual researcher and their supervisor
Individual researcher and their supervisor
21/4/2010
21/4/2010
21/4/2010
21/4/2010

If using UV light for detection, wear a full-face shield that is rated as
UV-proof, always aim the UV lamp away from your body, minimize the amount of time you need to have the UV turned on. Consult the Risk
Assessment for UV light sources also in this case.
Proper training of users
Individual researcher and their supervisor
Development of Standard Operating Procedure (SOP)
Supervisor of researcher
SMB Safety
Committee
Reading and following the SOP Individual user and their supervisor
Record the names of those consulted when deciding on risk control measures
Peter Kerr, Joe Dimauro, Jenny Phuyal, Zia Ahmad, Angela Nikolic, Robert Czolij, Craig Jackson
21/4/2010
21/4/2010
21/4/2010