OHS026
Safe Work Procedure
Faculty/Division
Medicine
Document number
SOMS GBD SWP 029
School/ Divisional Unit
School of Medical Sciences
Initial Issue date
13-03-2009
Current version
1.0
Current Version
Issue date
20-04-2010
Next review date
20-04-2013
The Writing Safe Work Procedures Guideline (OHS027) should be consulted to assist in the completion of this
form.
Safe Work Procedure Title and basic description
Mini organ bath
Title:
Description: use polygraph to record the tensions changes of the tissue in organ bath, measure mediators such as ATP that are
released from tissue under different conditions/stimulations.
Associated risk assessment title and location: RA working with electrical equipments 017, RA waste management 015 located in
Room 209B.
Describe the activity or process
Turn on computer and open POLYGRAPH program.
Turn on grass polygraphs.
Calibrate tensions of transducer using weights and powerlab.
Make up Kreb’s solution and bubble the solution with carbogen for 10-30mins.
Turn on the water-bath.
Place mini organ bath pump tubes into flask containing the Krebs and turn on pump to allow the Krebs to flow around the system.
Record the temperature, flow rate and bubble time (how long the bubble takes to flow though the system and reach the mini bath.
Adjust tubing length, water-bath temperature as necessary so that the flow rate is equal in all channels and the temperature of the
Krebs is 37°C on reaching the mini bath.
Dissect tissue strips under Helton hood and hang up the strips in the mini organ baths.
Adjust the tension to 1.0 g by tightening the strings.
Make the tissue to rest for 1 hour and start the experiment.
Use chemicals, mechanical or electrical stimulators to change the tension of the tissue and to record the tension changes by
POLYGRAPH.
Collect the Krebs after it passes through the tissue and store for analysis and assaying later.
Cut off the strips and thoroughly wash mini organ baths with water after the experiment finished by flushing the system.
Clean up the working area and wipe out the contaminated area and instruments with 70% ethanol and then with water.
Dispose all gloves, strings, tips, leftover tissue to biological waste bin and autoclaved bin.
List all resources required including plant,
chemicals, personal protective clothing and
equipment, etc



Suitable PPE,
Chemicals, peptides
Computer and grass polygraph
List potential hazards and risk controls including
specific precautions required



Tissue contaminated or infectious – all users wear suitable PPE, and always change gloves as soon as you have finished
handling tissue, use separate instruments that specially designated for anima tissue dissection.
Potential aerosol hazard – always use dissecting cabinet when handling human tissue, always wear suitable PPE.
Injured by dissecting instruments – All users are immunized against Hepatitis B, are trained to use instruments, notify the
first aid officer and go to see a doctor to get blood test immediately after injure.
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Page 1 of 2
Safe Work Procedure
Date Effective: 01/01/2007
Uncontrolled document when printed
Current Version: 1.2, 15/08/2007
List emergency shutdown instructions

Shut all electrical equipment off at power point in case of emergency.
List clean up and waste disposal requirements
Not applicable
List legislation, standards and codes of practice used
in the development of the SWP
NSW OHS Act 2000
NSW OHS Regulation 2001
Code of Practice for the Labeling of Workplace Substances
Australian Standard AS2243.6-1990. Safety in laboratories. Part 6: Mechanical Aspects.
Australian Standard AS2243.7-1991. Safety in laboratories. Part 7: Electrical Aspects.
Safe Work Procedure Form (OHS026)
Supervisory approval, training, and review
Supervisor: Prof E Burcher
Signature:
Plant custodian:
Signature
List competency required – qualifications, certificates, licensing, training - e.g. course or instruction:
SWP review date: 20-04-2013
Responsibility for SWP review: Fei Shang
___________________________________________________________________________________________________________
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Page 2 of 2
Safe Work Procedure
Date Effective: 01/01/2007
Uncontrolled document when printed
Current Version: 1.2, 15/08/2007