Long-chain alkanes: Allelochemicals for host location by the insect

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Long-chain alkanes: Allelochemicals for host location by the insect pest, Epilachna
dodecastigma (Coleoptera: Coccinellidae)
Journal name: Applied Entomology and Zoology
Nupur Sarkar · Abhishek Mukherjee · Anandamay Barik*
Ecology Research Laboratory, Department of Zoology, The University of Burdwan, Burdwan –
713 104, West Bengal, India
e-mail: anandamaybarik@yahoo.co.in
*corresponding author Tel.: 0342 2634200; Fax: 0342 2634200
(3A) Proportions of different synthetic alkanes mixed to prepare concentrations like young leaves of M. charantia
Synthetic
Concentration (µg)
alkane
12.5
25
50
100
200
400
n-C
15
0.03
0.05
0.10
0.21
0.42
0.83
n-C
16
0.05
0.10
0.21
0.42
0.83
1.67
n-C
17
0.09
0.17
0.34
0.69
1.38
2.75
n-C
18
0.12
0.25
0.49
0.98
1.97
3.94
n-C
19
0.14
0.28
0.56
1.12
2.23
4.46
n-C
20
0.16
0.32
0.64
1.28
2.56
5.13
n-C
21
0.13
0.27
0.53
1.07
2.13
4.26
n-C
22
0.15
0.30
0.60
1.21
2.41
4.82
n-C
23
0.11
0.23
0.46
0.91
1.82
3.64
n-C
24
0.09
0.18
0.36
0.72
1.44
2.88
n-C
25
0.19
0.39
0.78
1.55
3.10
6.21
n-C
26
0.10
0.21
0.41
0.82
1.64
3.28
n-C
27
1.78
3.56
7.11
14.23
28.45
56.90
n-C
28
0.23
0.47
0.93
1.87
3.73
7.47
n-C
29
2.00
4.00
8.01
16.02
32.04
64.08
n-C
30
0.15
0.30
0.60
1.20
2.39
4.79
n-C
31
3.65
7.29
14.59
29.18
58.36
116.71
n-C
32
0.25
0.50
1.00
2.00
4.01
8.01
n-C
33
1.13
2.26
4.51
9.02
18.04
36.09
n-C
36
1.38
2.76
5.51
11.02
22.05
44.09
Adding of individual alkane in each type of mixture of alkanes concentration will not be exactly equal because some alkanes were unidentified in
the natural extract.
(3B) Proportions of different synthetic alkanes mixed to prepare concentrations like mature leaves of M. charantia
Synthetic
Concentration (µg)
alkane
12.5
25
50
100
200
400
n-C
15
0.02
0.05
0.09
0.19
0.38
0.76
n-C
16
0.05
0.09
0.19
0.38
0.76
1.52
n-C
17
0.08
0.16
0.32
0.63
1.26
2.52
n-C
18
0.11
0.23
0.45
0.91
1.82
3.64
n-C
19
0.15
0.30
0.60
1.21
2.41
4.82
n-C
20
0.15
0.31
0.61
1.23
2.45
4.91
n-C
21
0.18
0.36
0.71
1.43
2.85
5.71
n-C
22
0.15
0.30
0.59
1.19
2.37
4.75
n-C
23
0.11
0.22
0.45
0.90
1.80
3.60
n-C
24
0.09
0.19
0.37
0.74
1.48
2.97
n-C
25
0.21
0.42
0.84
1.69
3.37
6.74
n-C
26
0.10
0.20
0.40
0.81
1.62
3.24
n-C
27
1.76
3.52
7.05
14.10
28.19
56.39
n-C
28
0.25
0.50
0.99
1.98
3.97
7.94
n-C
29
1.95
3.90
7.80
15.61
31.21
62.42
n-C
30
0.14
0.28
0.56
1.12
2.24
4.48
n-C
31
3.78
7.56
15.11
30.22
60.44
120.88
n-C
32
0.24
0.47
0.95
1.89
3.78
7.57
n-C
33
1.14
2.27
4.54
9.08
18.16
36.33
n-C
36
1.25
2.51
5.02
10.03
20.06
40.12
Adding of individual alkane in each type of mixture of alkanes concentration will not be exactly equal because some alkanes were unidentified in
the natural extract.
(3C) Proportions of different synthetic alkanes mixed to prepare concentrations like senescent leaves of M. charantia
Synthetic
Concentration (µg)
alkane
12.5
25
50
100
200
400
n-C
15
0.03
0.05
0.11
0.21
0.43
0.85
n-C
16
0.06
0.12
0.25
0.50
0.99
1.99
n-C
17
0.06
0.12
0.23
0.47
0.94
1.87
n-C
18
0.08
0.17
0.33
0.66
1.32
2.65
n-C
19
0.04
0.07
0.15
0.30
0.59
1.19
n-C
20
0.05
0.10
0.21
0.42
0.83
1.67
n-C
21
0.03
0.05
0.11
0.21
0.43
0.85
n-C
22
0.05
0.09
0.19
0.37
0.74
1.48
n-C
23
0.04
0.07
0.14
0.28
0.56
1.12
n-C
24
0.04
0.08
0.16
0.32
0.64
1.28
n-C
25
0.05
0.09
0.18
0.36
0.73
1.46
n-C
26
0.03
0.06
0.12
0.24
0.49
0.98
n-C
27
0.19
0.39
0.78
1.56
3.12
6.24
n-C
28
0.07
0.15
0.30
0.60
1.20
2.40
n-C
29
0.64
1.29
2.58
5.15
10.30
20.60
n-C
30
0.17
0.33
0.66
1.32
2.64
5.29
n-C
31
2.50
4.99
9.99
19.98
39.95
79.91
n-C
32
0.37
0.74
1.47
2.95
5.89
11.79
n-C
33
0.89
1.78
3.57
7.13
14.27
28.54
n-C
36
5.98
11.96
23.92
47.85
95.69
191.39
Adding of individual alkane in each type of mixture of alkanes concentration will not be exactly equal because some alkanes were unidentified in
the natural extract.
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