Scope and Sequence for 20___- 20___ School Year Instructor: TEA does not endorse any of the external products, vendors, consultants, or documentation referenced in this document. Any mention of vendors, products, or services is for informational purposes only. Cluster: Course Name: Agriculture, Food, and Natural Resources Advanced Biotechnology 130.364 (1 Credit) Course Description: Students enrolled in this course will apply advanced academic knowledge and skills to the emerging fields within biotechnology. This course is designed to develop essential knowledge and skills that will be expected by employers in the biotechnology industry. This course will afford students the opportunity to use sophisticated laboratory equipment, understand statistical analysis, and explore quality-control techniques. It is also the aim of this course to develop student scientific decision making skills, and critical thinking abilities. Course Prerequisites: Recommended mastery of Biology and Principles of AF&NR Course Requirements: The course is recommended for students in Grades 11-12 Recommended Facilities and Equipment: AFNR Facility Standards Guide Advanced Biotechnology Copyright © Texas Education Agency 2014. All rights reserved Week Number TEKS Taught Week 1 130.364c1A,B Week 2 130.364c1C,D Week 3 130.364c1E,F Week 4 130.364c1G,H, I Week 5 130.364c1J,K Week 6 130.364c9A,B Topic/Unit Title The student, for at least 40% of instructional time, conducts laboratory and field investigations using safe, environmentally appropriate, and ethical practices: The student will demonstrate safe practices during laboratory and field investigations. The student will demonstrate an understanding of the use and conservation of resources and the proper disposal or recycling of materials. The student, for at least 40% of instructional time, conducts laboratory and field investigations using safe, environmentally appropriate, and ethical practices: The student will demonstrate appropriate safety procedures, guidelines, and chemical hygiene plan; the student will maintain required safety training, including location an understanding of interpretation of material safety data sheets. The student, for at least 40% of instructional time, conducts laboratory and field investigations using safe, environmentally appropriate, and ethical practices: The student will comply with federal and state safety regulations as specified by Occupational Safety and Health Administration, and other regulatory agencies as appropriate. The student will also be able to identify and obey safety symbols and signs. The student, for at least 40% of instructional time, conducts laboratory and field investigations using safe, environmentally appropriate, and ethical practices: The student will maintain a clean and well organized work area, and dispose of equipment, glassware, and biologics according to laboratory policies. The student will be able to recognize common laboratory hazards. The student, for at least 40% of instructional time, conducts laboratory and field investigations using safe, environmentally appropriate, and ethical practices: The student will observe procedures for the safe use of instruments, gas cylinders, and chemicals. The student will be able to maintain safety and personal protection equipment. The student performs standard biotechnology laboratory procedures: The student will operate laboratory equipment such as a microscope, thermocycler, hood, pH meter, stirrers, balance, mixers, autoclave, power supply; practice measuring volumes and weights to industry standards. Advanced Biotechnology Copyright © Texas Education Agency 2014. All rights reserved The student prepares solutions and reagents for the biotechnology laboratory: The student will practice aseptic technique, prepare dispense, and monitor physical properties of stock reagents, buffers, media, and solutions. Week 7 130.364c9C Week 8 130.364c9D,E The student prepares solutions and reagents for the biotechnology laboratory: The student calculates and prepares a dilution series, and determines acceptability and optimum conditions of reagents for experimentation. 130.364c9F,G The student conducts quality control analysis while performing biotechnology laboratory procedures: The student will perform validation testing on laboratory reagents and equipment. The student will also analyze data, perform calculations, and run statistical analyses on results of quality control samples such as trending of data. 130.364c10A,B The student prepares solutions and reagents for the biotechnology laboratory: The student will practice aseptic technique; prepare, dispense, and monitor physical properties of stock reagents, buffers, media, and solutions. 130.364c10C,D The student prepares solutions and reagents for the biotechnology laboratory: The student calculates and prepares a dilution series, and determines acceptability and optimum conditions of reagents for experimentation. 130.364c12A,B The student conducts quality control analysis while performing biotechnology laboratory procedures: The student will perform validation testing on laboratory reagents and equipment. The student will also analyze data, perform calculations, and statistical analysis on results of quality control samples such as trending of data. Week 9 Week 10 Week 11 Week 12 Advanced Biotechnology Copyright © Texas Education Agency 2014. All rights reserved Week 13 Week 14 Week 15 Week 16 Week 17 Week 18 130.364c4A,B The student explores the emerging field of biotechnology: The student will define biotechnology as related to new and emerging occupations, explore engineering, and bioinformatics 130.364c4C,D The student explores the emerging field of biotechnology: The student will create a timeline of historical biotechnology research and development. The student will also research career opportunities in fields such as molecular, forensic, medical, regulatory, and agricultural biotechnology 130.364c5A,E The student analyzes bacterial, plant, and animal cell structures: The student will distinguish among bacterial, plant, and animal cells. The student will be able to identify cells using the microscope. 130.364c5B,C,D The student analyzes bacterial, plant, and animal cell structures: The student will describe the major structures in a bacterial, plant, and animal cell, and each of their respective functions. 130.364c6A,B 130.364c6C,D,E The student understands the role of genetics in the biotechnology industry: The student will explain terms related to molecular biology such as nucleic acids, nitrogen bases, amino acids, transcription, translation, polymerase, and protein synthesis. The students will also describe the structure of a nucleotide. The student understands the role of genetics in the biotechnology industry: The student will identify the nitrogen bases of deoxyribonucleic acid and ribonucleic acid. The student will explain how nucleotides join together to form a double-helical deoxyribonucleic acid molecule, and describe the deoxyribonucleic acid and ribonucleic acid replication process. Advanced Biotechnology Copyright © Texas Education Agency 2014. All rights reserved Week 19 130.364c6F,G The student understands the role of genetics in the biotechnology industry: The student will illustrate the process of protein synthesis, and define genome and gene expression. Week 20 130.364c6H,I The student understands the role of genetics in the biotechnology industry: The student will evaluate the significance of ethics and regulations as it relates to gene expression, and summarize the role of genetics in the biotechnology industry. 130.364c2A,B,C The student uses scientific methods during laboratory and field investigations: The student will know the definition of science, and understand it has limitations. The student will know that scientific hypotheses are tentative and testable statements that must be capable of being supported or not supported by observational evidence. The student is expected to understand hypotheses of durable explanatory power which have been tested over a wide variety of conditions are incorporated into theories. The student will know scientific theories are based on natural and physical phenomena and are capable of being tested by multiple independent researchers. Week 21 Week 22 130.364c2D,E Week 23 130.364c2F,G Week 24 130.364c2H,I,J The student uses scientific methods during laboratory and field investigations: The student will distinguish between scientific hypotheses and scientific theories. The student will also plan and implement investigative procedures, including asking questions, formulating testable hypotheses, and selecting, handling, and maintaining appropriate equipment and technology. The student uses scientific methods during laboratory and field investigations: The students will collect data individually or collaboratively, make measurements with precision and accuracy, record values using appropriate units, and calculate statistically relevant quantities to describe data. The students will also demonstrate the use of course apparatus, equipment, techniques and procedures. The student uses scientific methods during laboratory and field investigations: The student will organize, analyze, evaluate, build models, make inferences, and predict trends from data. The student will also be able to perform calculations using dimensional analysis, significant digits, and scientific notation. The student will also be able to communicate valid conclusions using essential vocabulary and multiple modes of expression such as lab reports, etc. Advanced Biotechnology Copyright © Texas Education Agency 2014. All rights reserved Week 25 130.364c3A,B Week 26 130.364c3C,D Week 27 Week 28 Week 29 Week 30 The student uses critical thinking, scientific reasoning, and problem solving to make informed decisions within and outside the classroom: The student will analyze and evaluate scientific explanations using empirical evidence, logical reasoning and experimental and observation testing. The student will also communicate and apply scientific information extracted from various sources. The student uses critical thinking, scientific reasoning, and problem solving to make informed decisions within and outside the classroom: The student will draw inferences based on data related to promotional materials for products and services, and evaluate the impact of research and technology on scientific thought, society, and the environment. 130.364c3E,F The student uses critical thinking, scientific reasoning, and problem solving to make informed decisions within and outside the classroom: The student will describe the connection between biotechnology and future careers, and research and describe the history of biotechnology and contributions of scientists. 130.364c7A,B The student analyzes the importance of recombinant deoxyribonucleic acid technology and genetic engineering: The student will define recombinant deoxyribonucleic acid technology as it relates to the biotechnology industry; explain how recombinant deoxyribonucleic acid technology is used to clone genes. 130.364c7C,D,E The student analyzes the importance of recombinant deoxyribonucleic acid technology and genetic engineering: The student will explain the role of tissue cultures to genetic modification procedures, propagate plant cultures, and maintain proper growing conditions for plant tissue cultures. 130.364c7F,G The student analyzes the importance of recombinant deoxyribonucleic acid technology and genetic engineering: The student will explain the role of restriction enzymes and plasmid deoxyribonucleic acid, and describe the vector commonly used, including bacteriophage vectors. Advanced Biotechnology Copyright © Texas Education Agency 2014. All rights reserved Week 31 Week 32 Week 33 Week 34 Week 35 Week 36 130.364c7H,I The student analyzes the importance of recombinant deoxyribonucleic acid technology and genetic engineering: The student will discuss the polymerase chain reaction and its application in recombinant deoxyribonucleic acid technology, and perform restriction digests. 130.364c8A,B The student examines federal, state, local, and industry regulations as related to biotechnology: The student will discuss the relationship between local, state, and federal agencies responsible for regulation of the biotechnology industry, and analyze policies and procedures sued in the biotechnology industry such as animal research laboratories. 130.364c11A,B,C The student performs advanced biotechnology laboratory procedures: The student will explain the importance of media components to the outcome of cultures; isolate, maintain, and store pure cultures; prepare seed inoculum. 130.364c11D,E The student performs advanced biotechnology laboratory procedures: The student will perform plating techniques such as the Kirby-Bauer method, and precipitate and solubilize proteins. 130.364c11F,G The student performs advanced biotechnology laboratory procedures: The student will isolate and interpret proteins using electrophoresis, and perform nucleic acid sequencing procedures. 130.364c13A,B,C The student summarizes biotechnology laboratory procedures and their applications in the biotechnology industry: The student will identify major sectors of the biotechnology industry, categorize the biotechnology laboratory procedures included in each sector, and compare the different applications used in biotechnology Revised by: Ian Sprouse, Texas A&M University. Advanced Biotechnology Copyright © Texas Education Agency 2014. All rights reserved