Electronic Supplementary Material

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Electronic Supplementary Material
Microwave-assisted synthesis of BSA-modified silver nanoparticles as a selective
fluorescent probe for detection and cellular imaging of cadmium (II)
Yu Gu a, Nan Lia, Mengmeng Gaoa, Zilu Wanga, Deli Xiaoa, Yun Lia, Huning Jia a,*, Hua
Hea,b,*
a
Division of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China
b
Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical
University), Ministry of Education, China
*
To whom correspondence should be addressed: E-mail addresses: dochehua@163.com (Hua
He) and jiahuning@sina.com (Huning Jia)
Optimizations for Synthesis the BSA-Ag NPs
Fig.S1 shows the fluorescence emission spectrum of AgNPs products synthesized under
a 20 s, 40 s, 60 s, 80 s, 100 s, 120 s, 140 s, 160 s, 180 s, 200 s of MW heating. It is observed
that the solution change from colorless to transparent yellow to dark yellow (inset of figS1.b-e)
and the fluorescence emission peaks occurred slightly red-shift indicating the formation of
larger number of AgNPs with increased heating time. The transmission electron microscope
(TEM) shows the form of AgNPs after Microwave heated 60 s. 60 s MW heating was applied
to our subsequent MW-assisted synthesis of BSA–AgNPs as it produced the highest
fluorescence intensity (Fig.S2). Fig.S3 shows the changes in the fluorescence intensity of
BSA-AgNPs when adding different amount of AgNO3. The results indicated that with an
increase of AgNO3 concentration, a maximum value of fluorescence intensities was obtained
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for 15 mM AgNO3, finally adding 15 mM AgNO3, MW heating 60 s were chosen in our
experiment.
Influence of microwave heated time on the fluorescence emission spectra
Fig.S1 Influence of microwave heated time on the fluorescence emission spectra. Inset a: the
solid form of BSA-Ag NPs by freeze-drying. b: the color of BSA aqueous solutions. c-e: the
color of BSA-AgNPs aqueous solutions by microwave heating 60 s, 160 s, 100 s.
Influence of microwave heated time on the fluorescence intensities
Fig.S2 Influence of microwave heated time on the fluorescence intensities at the maximum
emission wavelength (excited at 465 nm).
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Influence of AgNO3 on the fluorescence intensities
Fig.S3 Influence of AgNO3 on the fluorescence intensities at the maximum emission
wavelength (excited at 465 nm).
Ag 3d XPS spectrum of BSA-AgNPs
Fig.S4 Ag 3d XPS spectrum of BSA-AgNPs.
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