Electronic Supplementary Material Microwave-assisted synthesis of BSA-modified silver nanoparticles as a selective fluorescent probe for detection and cellular imaging of cadmium (II) Yu Gu a, Nan Lia, Mengmeng Gaoa, Zilu Wanga, Deli Xiaoa, Yun Lia, Huning Jia a,*, Hua Hea,b,* a Division of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China b Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, China * To whom correspondence should be addressed: E-mail addresses: dochehua@163.com (Hua He) and jiahuning@sina.com (Huning Jia) Optimizations for Synthesis the BSA-Ag NPs Fig.S1 shows the fluorescence emission spectrum of AgNPs products synthesized under a 20 s, 40 s, 60 s, 80 s, 100 s, 120 s, 140 s, 160 s, 180 s, 200 s of MW heating. It is observed that the solution change from colorless to transparent yellow to dark yellow (inset of figS1.b-e) and the fluorescence emission peaks occurred slightly red-shift indicating the formation of larger number of AgNPs with increased heating time. The transmission electron microscope (TEM) shows the form of AgNPs after Microwave heated 60 s. 60 s MW heating was applied to our subsequent MW-assisted synthesis of BSA–AgNPs as it produced the highest fluorescence intensity (Fig.S2). Fig.S3 shows the changes in the fluorescence intensity of BSA-AgNPs when adding different amount of AgNO3. The results indicated that with an increase of AgNO3 concentration, a maximum value of fluorescence intensities was obtained 1 for 15 mM AgNO3, finally adding 15 mM AgNO3, MW heating 60 s were chosen in our experiment. Influence of microwave heated time on the fluorescence emission spectra Fig.S1 Influence of microwave heated time on the fluorescence emission spectra. Inset a: the solid form of BSA-Ag NPs by freeze-drying. b: the color of BSA aqueous solutions. c-e: the color of BSA-AgNPs aqueous solutions by microwave heating 60 s, 160 s, 100 s. Influence of microwave heated time on the fluorescence intensities Fig.S2 Influence of microwave heated time on the fluorescence intensities at the maximum emission wavelength (excited at 465 nm). 2 Influence of AgNO3 on the fluorescence intensities Fig.S3 Influence of AgNO3 on the fluorescence intensities at the maximum emission wavelength (excited at 465 nm). Ag 3d XPS spectrum of BSA-AgNPs Fig.S4 Ag 3d XPS spectrum of BSA-AgNPs. 3