Embryo cryopreservation through IVF Project Flow Chart
Transfer mice
to TMF
PPMS
Week 1
Order female
donor
Week 1
IVF
Procedure
Week 3
Quality
control test
Week 4
Genotype
Pups
Week 8
Quick Guide (see below for more detailed flow chart):
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PI submits a project request form in PPMS
PI transfers 2 males for IVF procedure to Peds G08 (week1)
IVF procedure is done, resulting 2-cell embryos are frozen down (week 3)
TMF thaws one straw for quality control and implants embryos into recipient female (week 4)
PI genotypes toe biopsies from pups born and sends results to the Core (week 8)
Pups are euthanized or weaned onto PI’s smart key and protocol number and transferred to PI’s housing location.
Detailed Guide:
Before Project Begins:
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PI is responsible to order the project from PPMS
PI needs contact TMF to discuss project
PI is responsible to transfer proven breeder male mice to TMF
Project Flow:
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PI lab is responsible for transfer proven breeder male mice 10-16 weeks old to Peds G08
TMF will order 15 wild type female egg donors with PI’s smart key.
TMF will perform IVF procedure and cryopreserve resulting 2-cell embryos in straws.
Quality control test: One straw from each freezing session will be thawed and the embryos will be implanted into a recipient female
Toe clips will be taken from the resulting pups for genotyping when pups are 7 days old
PI lab is responsible for screening pups from toe biopsies
PI lab is responsible to send TMF screening result and electronic image
When pups are 4 weeks old, TMF will use PI’s IACUC protocol and smart key to wean the pups
After genotyping confirmed, TMF staff will euthanasia pups or transfer to PI lab.
Service Rate Structure:
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TMF will bill PI on the month of the project initiated:
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Mouse embryo cryopreservation via IVF fee: $1963
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Purchase of wildtype embryo donor females will be ordered directly on PI’s smart key
Extra fee will be billed to PI lab in the following cases:
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Per diem fee after pups wean
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$38 per hour plus material cost when TMF extract DNA from tail samples
Project Guarantee and Quality Control
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TMF will guarantee live mice with correct genotype
TMF will guarantee the existing Core protocol and Core SOP are followed, and will follow any protocols provided from PI