AuroFlow™ Fluoroquinolone Strip Test Kit for Milk 1102-01 96 strips
AuroFlow™ Fluoroquinolone Strip Test Kit for Milk 1102-02 192 strips
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The detection limits of this kit meet all of the EU MRLs for fluoroquinolones
Rapid strip test method – 7 minutes
Broad-spectrum antibody that requires no heating step
Works in cold milk
No sample preparation
High sensitivity
High reproducibility
Interpretation of results can be done by eye or using the QuickSTAR™ Strip Reader
The AuroFlow™ Fluoroquinolone Strip Test Kit for Milk is a qualitative and rapid lateral flow assay designed to detect fluoroquinolone antibiotic residues in raw
commingled cow’s milk. This state-of-the-art test uses a broad-spectrum antibody capable of specifically detecting a range of fluoroquinolone residues. This test is
designed for rapid field use or reference lab settings and eliminates the need for a heating block.
The assay uses a competitive colloidal gold based format. The milk sample is added to a clear plastic reaction vessel, and used to resuspend the lyophilized
reagents to a uniform pink color in the bottom of the microtiter wells. The milk is incubated briefly (3 min) to allow the fluoroquinolone antibody on the gold particles
to engage with any fluoroquinolone antibiotic residues present in the milk. The test strip is then inserted into the sample well with the arrows pointing downward
initiating capillary flow up the strip. Any gold particles that are not complexed with antibiotics present in the milk will bind to the fluoroquinolone antibiotic imprinted
at the Test line (T-line), forming a signal (red line) at that position. If the fluoroquinolone antibody on the gold particle has engaged with antibiotic present in the
milk sample, the gold particle will flow past the T-line and reach the Control line (C-line). For visual interpretation of the test results, T-line signal intensity that is
stronger than the signal at the C-line indicates a negative result. Signal at the T-line which is equal or less intense compared to the C-line indicates the presence of
fluoroquinolone antibiotics in the milk. The greater the reduction in signal intensity at the T-line, the greater the concentration of fluoroquinolone antibiotic residues
present in the milk.
Fluoroquinolones are a group of antibiotics that can inhibit the activity of bacterial DNA gyrase. The fluoroquinolone group mainly includes enrofloxacin and its
active metabolite ciprofloxacin, danofloxacin, difloxacin, norfloxacin, pipemidic acid, ofloxacin, benofloxacin and others. One of widely used fluoroquinolones is
enrofloxacin, which was the first fluoroquinolone antimicrobial to be used in veterinary infections by E. coli, Salmonella, Pasteurella, Mycoplasma and Hemophilus
species. The MRLs for the sum of enrofloxacin and its active metabolite ciprofloxacin have been stated at 100 μg/kg (ppb) for muscle tissue and fat, and 100 μg/kg
(ppb) for milk.
This kit is manufactured to the international quality standard ISO 9001:2008. ISO CI#: SARA-2009-CA-0114-02-A
Kit Specs
The AuroFlow™ Fluoroquinolone Strip Test Kit for Milk has the capacity for 96 (Cat # 1102-01) or 192 (Cat # 1102-02) determinations. The shelf life is 24 months
when the kit is properly stored.
The positive and negative control vials contain lyophilized raw milk samples, and must be reconstituted with 1 mL of distilled water before use. The reconstituted
raw milk controls are stable for 48 hours at 4°C. Additional positive and negative control vials are available separately (Cat # 110210).