B2 High Demand Application Questions 163 marks= 167 minutes Q1. Changing the conditions in which plants grow affects how fast they grow. The diagram shows a propagator in which scientists can control temperature, light intensity and carbon dioxide concentration. The graph shows the effects of changing the temperature, light intensity and carbon dioxide concentration on the growth of lettuce plants. Page 1 (a) Describe and explain the effect of increasing light intensity on the mean mass of lettuce plants at 4% carbon dioxide and 15 °C. .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... (3) (b) Growers wish to make maximum profits from their lettuces. What do they need to consider before making decisions about the growing conditions for their lettuces? .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... (2) (c) The nutrient solution contains nitrate ions and magnesium ions. Complete the table to show the functions of these ions in plants and their deficiency symptoms. Ion Function in plants Deficiency symptoms ......................................................... ........................................................ Nitrate ......................................................... ........................................................ ......................................................... ......................................................... ......................................................... .......................................................... Magne ......................................................... sium ......................................................... ......................................................... ......................................................... Page 2 (4) (Total 9 marks) ## The following is adapted from an article which appeared in January 1994 in the Sunday Times. A woman gives birth at 59 Designer babies are just the first step in science’s ability to create a super race. Test-tube baby techniques give doctors an unprecedented degree of control over the offspring produced. When eggs and sperms are donated they seek to match the charactaristics of the donor whose role has been replaced. As more is learned about the genetic contribution to charactaristics such as intelligence, beauty or strength, this capacity to mix-and-match will increase. Technological change frightens us when it is outside our control and when we fear how it might be exploited by others. People wonder where the potential Brave New World of “retirement births” and ‘designer’ babies will ultimately lead us: cloning, genetic grading and even the introduction of animal genes into humans. Unthinkable, perhaps, but entirely possible. The techniques are there – in fact they have been for years. But who on earth would want to use them, apart from some power mad politician? Use information from the article and any of your own knowledge to discuss the arguments for and against allowing doctors to experiment with human fertilisation techniques. You will not receive marks for simply copying extracts from the article. (Total 8 marks) Q3. (a) During respiration, sugar is oxidised to release energy. Complete the equation for respiration. Sugar + ............................. = ............................. + ............................. + energy (3) Page 3 (b) The photograph below shows an athlete using an exercise machine. The machine can be adjusted to vary the rate at which the athlete is required to work. The athlete’s heart rate and breathing rate were measured at different work rates. The table below shows the results which were obtained. WORK RATE (J/s) HEART RATE (beats/min.) BREATHING RATE (breaths/min.) 0 86 9.6 60 106 10.0 80 112 10.4 100 122 10.4 120 135 11.4 140 143 14.5 160 156 15.8 200 174 30.5 Plot the data on the graph paper below. Page 4 (3) (c) Explain, as fully as you can, the advantages to the body in the change in breathing and heart rates. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... Page 5 ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (6) (d) This increase in the rate of heart-beat is a response to a stimulus. For this response suggest: (i) the stimulus; .......................................................................................... (ii) the co-ordinator; .......................................................................................... (iii) the effector. .......................................................................................... (3) (Total 15 marks) Q4. The graph shows the effect of temperature on photosynthesis. Page 6 (a) Between which temperatures is the rate of photosynthesis fastest? ............................... and .......................... °C (1) (b) Suggest why the rate of photosynthesis stays the same between these two temperatures. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (2) (c) A greenhouse owner wants to grow lettuces as quickly and cheaply as possible in winter. At what temperature should he keep his greenhouse in order to grow the lettuces as quickly and cheaply as possible? .......................... °C Page 7 Explain your answer. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (3) (Total 6 marks) Q5. The graph shows the mean light intensity at different times of the year in an oak wood. (a) (i) In which month would you expect the rate of photosynthesis in the oak trees to be greatest? ......................................................................... (1) (ii) There are plants living on the ground in the wood. In which month would you expect their rate of growth to be fastest? ........................................................................................................................... Explain your answer. ........................................................................................................................... Page 8 ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (3) (b) Name two factors, other than light intensity, that would affect the rate of photosynthesis in the oak trees. 1 .................................................................................................................................. 2 .................................................................................................................................. (2) (Total 6 marks) ## The passage below is an extract from an article in The Independent newspaper. This Mouthwash could mean a lot to your Children A SIMPLE TEST SCREENS WOULD-BE PARENTS FOR THE DEFECTIVE GENE THAT CAUSES CYSTIC FIBROSIS It is not every week that a university professor tells you to spit in front of him. But Bob Williamson, professor of biochemistry and molecular genetics asked me to do just that. In future, some people may choose their marriage partners on the basis of the contents of their spit. The professor handed me a sample bottle containing sterile salt solution. I rinsed my mouth out and spat back into the tube. Incredible though it may sound, the mouthwash now contained enough cells from the inner lining of my cheek for an assistant to dissect my DNA and check whether I carry any of the mutations responsible for cystic fibrosis. Professor Williamson’s team can locate genetic markers, distinctive segments of DNA, that are inherited along with the mutant genes in people affected with cistic fibrosis. About 16 000 people who bought The Independent this morning unwittingly carry a cystic fibrosis gene. The statistics indicate that 23 of the staff of this newspaper are unknowing carriers. Carriers are normal healthy individuals who do not have the Page 9 disease. (a) Describe, as fully as you can, where genes are located inside cheek cells. ..................................................................................................................................... ..................................................................................................................................... (2) (b) The gene for cystic fibrosis has two forms called alleles. Only the recessive allele causes cystic fibrosis. Explain how two healthy carriers of the cystic fibrosis allele could produce a child with the disease. Use the symbol A for the normal allele of the gene and a for the allele which produces the disease. You may use a diagram if you wish. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (4) (d) In the test used to identify the ‘genetic markers’, DNA is extracted from the cheek cells. The DNA molecules are then made to produce hundreds of millions of copies of themselves. (i) Explain, as fully as you can, how the structure of DNA molecules allows them to replicate themselves. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... Page 10 ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (6) (ii) Explain how DNA controls the structure of proteins. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (3) (Total 15 marks) Q7. (a) Mice with black fur can have the genotype BB or Bb, whilst mice with brown fur have the genotype bb. (i) Use a genetic diagram to show what fur colours you would predict in the F1 offspring produced by two mice who are both Bb. (3) (ii) Why might your prediction of fur colour in the F1 generation not be proved right? ........................................................................................................................... ........................................................................................................................... Page 11 (1) (b) Using the example in part (a) to help: (i) describe the difference between dominant and recessive alleles; ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (2) (ii) describe the difference between alleles and genes; ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (2) (iii) describe the difference between homozygous and heterozygous chromosomes. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (2) (Total 10 marks) Q8. Person A and Person B measured their pulse rates over a period of five minutes. For one minute of this time they exercised by stepping on and off a box. At other times they sat Page 12 still. The graph shows the results for Person A. (i) What does the graph tell you about the changes in the pulse rate of Person A within the five minute period? ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (3) (ii) What was the pulse rate of Person A at the end of the five minute period? ..................................................................................................................................... (1) (iii) The table shows the results obtained for Person B. Time in minutes Pulse rate per minute Page 13 0 68 1 68 2 110 3 96 4 80 5 68 Plot these results on the graph. (2) (Total 6 marks) Q9. (a) The volume of blood pumped out of the left ventricle at each beat was measured for a person during six different activities. These activities showed an increasing energy demand, with rest requiring the least energy and rowing a boat the most. The results of these measurements are shown on the bar chart. Page 14 (i) The pulse rate was also measured for the person during the same activities. The table shows the results that were obtained. Activity Pulse rate in beats per minute Rest 70 Writing 85 Cleaning the floor 100 Wallpapering 120 Walking fast 132 Rowing a boat 153 On the graph paper below draw a bar chart of the results obtained for the measurements of the pulse rate. (2) (ii) Undertaking activities with increasing energy demand has an effect on the volume of blood pumped from the left ventricle (per beat) and on the pulse rate. What do the bar charts show these effects to be? Use only information shown in Page 15 the bar charts in your answer. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (2) (b) The pulse rate changed when the activity changed. Explain the reason for this. .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... (2) (Total 6 marks) Q10. Photosynthesis takes place the leaves of green plants. (a) Write a balanced chemical equation for the formation of glucose by photosynthesis. .................................................................................................................................... (3) (b) Describe two ways that the rate of photosynthesis can be decreased without lowering the temperature. .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... (2) (c) Some students decided to investigate the effect of temperature on the rate of Page 16 photosynthesis in pond weed. They set up the apparatus and altered the temperature using ice and hot water. The counted the number of bubbles given off in a minute at different temperatures. They obtained the following results. (i) Plot the points on the graph. (3) (ii) Use your graph to predict the number of bubbles per minute at 25 °C. .......................................................................................................................... (1) Page 17 (iii) Suggest a reason why the rate of photosynthesis seems to decrease in this pondweed after 40 °C. .......................................................................................................................... .......................................................................................................................... (1) (Total 10 marks) Q11. The diagram shows a human sperm. Inside the tail of the sperm is a filament mechanism that causes the side to side movement of the tail, which moves the sperm. (a) Describe the function of the mitochondria and suggest a reason why they are arranged around the filament near the tail of the sperm. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (3) (b) Explain the significance of the nucleus in determining the characteristics of the offspring. ..................................................................................................................................... Page 18 ..................................................................................................................................... ..................................................................................................................................... (2) (Total 5 marks) Q12. Regular exercise is important, as it helps to maintain an efficient supply of blood to the muscles, the heart and the lungs. This is helped by an increase in the heart rate during exercise. Explain why it is necessary for the heart rate to increase during exercise. ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... (Total 4 marks) Q13. A student‘s breathing was monitored before and after vigorous exercise. The student breathed in and out through a special apparatus. The graphs show the changes in the volume of air inside the apparatus. Each time the student breathed in, the line on the graph dropped. Each time the student breathed out, the line went up. Page 19 (a) How many times did the student breathe in per minute: Page 20 before exercise; ........................................................................................................... after exercise? ............................................................................................................. (1) (b) On each graph, the line A – B shows how much oxygen was used. The rate of oxygen use before exercise was 0.5 dm3 per minute. Calculate the rate of oxygen use after exercise. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... Rate of oxygen use after exercise = ................................................. dm3 per minute (2) (c) The breathing rate and the amount of oxygen used were still higher after exercise, even though the student sat down to rest. Why were they still higher? ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (4) (Total 7 marks) Q14. The fruit fly, Drosophila, has either long wings or vestigial wings, as shown in the diagram. Page 21 Long-winged fly Vestigial-winged fly The size of the wings is determined by a pair of alleles: A and a. Long-winged flies have one of two possible genotypes: AA or Aa. Vestigial-winged flies have only one genotype: aa. (a) (i) What is the genotype of a heterozygous fly? ........................................................................................................................... (1) (ii) Why can vestigial-winged flies only have the genotype aa? ........................................................................................................................... (1) (b) A male and a female long-winged fly were crossed. They produced 96 offspring. 72 of the offspring had long wings and 24 had vestigial wings. Use a genetic diagram to explain this. (4) (Total 6 marks) Page 22 Q15. The diagram shows two patterns of cell division. Cell division type A is used in gamete formation. Cell division type B is used in normal growth. (a) Name the two types of cell division, A and B, shown in the diagram. Type A ........................................................................................................................ Type B ........................................................................................................................ (2) (b) Name the process in which an egg and sperm join together. ............................................................................................................................. ........ (1) Page 23 (c) Cell 1 contains 46 chromosomes. How many chromosomes will there be in: (i) cell 10; .............................................................................................................. (1) (ii) cell 14? ............................................................................................................. (1) (Total 5 marks) Q16. The diagram shows the apparatus used to investigate the digestion of milk fat by an enzyme. The reaction mixture contained milk, sodium carbonate solution (an alkali) and the enzyme. In Experiment 1, bile was also added. In Experiment 2, an equal volume of water replaced the bile. In each experiment, the pH was recorded at 2-minute intervals. Either: Experiment 1 or: milk (contains fat) sodium carbonate solution bile enzyme Experiment 2 milk (contains fat) sodium carbonate solution water enzyme The results of the two experiments are given in the table. Page 24 (a) Milk fat is a type of lipid. Give the name of an enzyme which catalyses the breakdown of lipids. ............................................................................................................................. ...... (1) (b) What was produced in each experiment to cause the fall in pH? ............................................................................................................................. ...... (1) (c) (i) For Experiment 1, calculate the average rate of fall in pH per minute, between 4 minutes and 8 minutes. Show clearly how you work out your final answer. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ............................................. pH units per minute (2) (ii) Why was the fall in pH faster when bile was present? ........................................................................................................................... Page 25 ........................................................................................................................... (1) (Total 5 marks) Q17. The graph below shows the effect of a high carbohydrate diet on the stored carbohydrate in the muscles. Diet A – Diet B Diet C – – High carbohydrate diet, started after several days of eating a diet without carbohydrate. High carbohydrate diet, started after normal mixed diet. Normal mixed diet. What advice would you give the athlete about the best diet preparation for a long race? Explain why you would give this advice. Diet ...................................................................... Explanation Page 26 ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... (Total 2 marks) Q18. Changing the conditions in which plants grow affects how fast they grow. The diagram shows a propagator in which scientists can control temperature, light intensity and carbon dioxide concentration. The graph shows the effects of changing the temperature, light intensity and carbon dioxide concentration on the growth of lettuce plants. Page 27 (a) Describe and explain the effect of increasing light intensity on the mean mass of lettuce plants at 4% carbon dioxide and 15 °C. .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... (3) (b) Growers wish to make maximum profits from their lettuces. What do they need to consider before making decisions about the growing conditions for their lettuces? .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... Page 28 .................................................................................................................................... (2) (c) The nutrient solution contains nitrate ions and magnesium ions. Complete the table to show the functions of these ions in plants and their deficiency symptoms. Ion Function in plants Deficiency symptoms ......................................................... ........................................................ Nitrate ......................................................... ........................................................ ......................................................... ......................................................... ......................................................... .......................................................... Magne ......................................................... sium ......................................................... ......................................................... ......................................................... (4) (Total 9 marks) Q19. The diagram shows the apparatus used to investigate the digestion of milk fat by an enzyme. The reaction mixture contained milk and the enzyme. In Experiment 1, bile was also added. In Experiment 2, an equal volume of water replaced the bile. In each experiment, the pH was recorded at 2 minute intervals. Page 29 The results of the two experiments are given in the table. pH Time in minutes (a) Experiment 1: with bile Experiment 2: without bile 0 9.0 9.0 2 8.8 9.0 4 8.7 9.0 6 8.1 8.8 8 7.7 8.6 10 7.6 8.2 Milk fat is a type of lipid. Explain why the pH changed in experiment 2. ........................................................................................................................ ........................................................................................................................ ........................................................................................................................ ........................................................................................................................ (2) Page 30 (b) (i) For Experiment 1, calculate the average rate of fall in pH per minute, between 4 minutes and 8 minutes. Show clearly how you work out your final answer. ............................................................................................................... ............................................................................................................... ................................. pH units per minute (2) (ii) The average rate of fall in pH per minute for Experiment 2 was 0.1 units of pH per minute. Explain the difference in the rate of fall in pH when bile is present. ............................................................................................................... ............................................................................................................... (1) (Total 5 marks) Q20. DNA fingerprinting can be used to identify people. One example of the use of DNA fingerprinting is to find out which man is the father of a child. The diagram shows the DNA fingerprints of a child, the child’s mother and two men who claim to be the child’s father. The numbers refer to the bars on the DNA fingerprints. Page 31 (a) Only half the bars of the child’s DNA fingerprint match the mother’s DNA fingerprint. Explain why. ........................................................................................................................ ........................................................................................................................ (2) (b) Which man, A or B, is more likely to be the father of the child? Use the numbers on the DNA fingerprints to explain your choice. In your answer you should refer to all four people. ........................................................................................................................ ........................................................................................................................ ........................................................................................................................ ........................................................................................................................ ........................................................................................................................ ........................................................................................................................ (3) (Total 5 marks) Page 32 Q21. The diagram shows a family tree in which some individuals have an inherited disorder, which may cause serious long-term health problems. (a) What proportion of the children of A and B have the disorder? ..................................................................................................................................... (1) (b) Explain the evidence from the diagram which shows that the allele for the disorder is dominant. Use the appropriate letters to identify individuals in your answer. You may use genetic diagrams in your explanation. There is space for you to draw a genetic diagram at the top of the facing page. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... Page 33 ..................................................................................................................................... ..................................................................................................................................... (3) (c) (i) What is meant by ‘embryo screening’? ........................................................................................................................... ........................................................................................................................... (1) (ii) A doctor suggests that couple C and D should have their embryos screened but that couple G and H do not need this procedure. Explain the reasons for the doctor’s suggestions. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (3) (Total 8 marks) Q22. The dodo is an extinct bird. The drawing shows an artist’s impression of the bird. Page 34 The dodo lived on a small island in the middle of the Indian Ocean. Its ancestors were pigeon-like birds which flew to the island millions of years ago. There were no predators on the island. There was a lot of fruit on the ground. This fruit became the main diet of the birds. Gradually, the birds became much heavier, lost their ability to fly and evolved into the dodo. (a) Suggest an explanation for the evolution of the pigeon-like ancestor into the flightless dodo. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (4) (b) The dodo became extinct about 80 years after Dutch sailors first discovered the island in the eighteenth century. Scientists are uncertain about the reasons for the dodo’s extinction. Suggest an explanation for this uncertainty. ..................................................................................................................................... Page 35 ..................................................................................................................................... (1) (Total 5 marks) Q23. Bile is produced in the liver, stored in the gall bladder, then released into the small intestine. (a) Explain how bile affects the digestion of food in the small intestine. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (2) (b) Bile contains bile pigments and cholesterol. If the diet contains too much cholesterol, some of it may form ‘gallstones’ in the bile. These gallstones may prevent bile from moving out of the gall bladder into the small intestine. Bilirubin is a yellow-brown bile pigment. This pigment is produced by the liver from haemoglobin released by broken-down red blood cells. Suggest how gallstones may produce the following symptoms: (i) very pale faeces ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (2) (ii) jaundice (a yellow tinge to the skin). ........................................................................................................................... Page 36 ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (2) (Total 6 marks) Page 37 M1. (a) any three from: • ((mean) mass) increases up to 7 / 8 units (of light) then levels off • light limiting factor up to 7 / 8 units • for photosynthesis must be in correct context • other factor / temperature limiting above 7 / 8 units 3 (b) any two from: • cost of providing conditions / heat / light / CO2 • effect of treatment on profit allow too much of factor is wasteful • relevant use of data from graph eg limiting factors • named other factors eg fertiliser / pest control / weeds / density of planting allow taste / appearance 2 (c) nitrate function produce amino acids / proteins / enzymes ignore DNA do not allow chlorophyll 1 nitrate deficiency stunted growth allow description ignore plant dies 1 magnesium function produce chlorophyll ignore chloroplasts 1 magnesium deficiency yellow leaves / plant Page 38 ignore plant dies 1 [9] M2. Cogently argued based on biological principles, for and against experimenting with human fertilisation techniques max 4 pros e.g. help for women who cannot conceive naturally possibility of eliminating disease causing genes possibility of inserting useful animal genes max 4 cons e.g. ethical objections religious objections social issues any 6 for 1 mark each 6 cogently argued case gains up to 2 marks 2 [8] M3. (a) oxygen; ) carbon dioxide; ) allow symbols water ) each for 1 mark 3 (b) graph with reasonable vertical scales; accurate plotting of all points (ignore lines) and labelling lines histogram – must be coded gains 3 marks 3 (c) 6 of: during exercise the level of CO2 (in the blood) rises; Page 39 increased breathing to remove excess CO2; increased oxygen supply to muscles; or increased breathing takes in more O2 or increased heart rate takes more O2 to muscles; increased supply of sugar to muscles; increased respiration rate; enable faster rate of energy release; reference to lactic acid (allow even though not on syllabus)/O2 debt; to avoid cramp; anaerobic reference; reference to removal of ‘heat’; 6 (d) high carbon dioxide concentration; brain/central nervous system; heart muscles (both) 3 [15] M4. (a) 21.5 – 22 and 27 – 27.5 for 1 mark 1 (b) ideas of limiting factor / shortage of e.g. light / carbon dioxide / water / chlorophyll each for 1 mark (allow 1 for ‘maximum / optimum rate of enzyme activity if no reference to limiting factors) (ignore denaturation) 2 (c) 21.5 – 22° C (allow first figure from answer to (i) so that no ‘double-penalty but only if this first answer is 20 or greater) maximum rate of photosynthesis / highest / fastest but related to flat part of curve most economical heating / cheapest related to heating must relate to the temperature the candidate has given each for 1 mark Page 40 3 [6] M5. (a) (i) June for 1 mark 1 (ii) April max. light photosynthesis makes sugars/substances needed for growth for 1 mark each 3 (b) 2 of: temperature carbon dioxide availability water chlorophyll any 2 for 1 mark each 2 [6] M6. (a) on chromosomes/DNA within the nucleus each for 1 mark 2 (b) parental genotypes correct i.e. Aa Aa; gamete genotypes correct i.e. A or a A or a/correct lines; F1 genotypes correct i.e. AA Aa Aa aa; aa recognised as child with cystic fibrosis each for 1 mark 4 Page 41 (d) (i) molecule has two long strands/double helix; idea of held together by (weak) bonds; each strand has 4 different types of base; ) which pair with specific bases in opposite strand; ) when strands separate; ) OWTTE each strand acts as a ‘complementary’ template; ) makes 2 identical strands ) each for 1 mark 6 (ii) order of bases acts as a code; which controls the order; in which amino acids are assembled into protein; read in triplet each for 1 mark 3 [15] M7. (a) (i) gametes i.e. B b and B b 1 correct combination of genotypes i.e. BB, Bb, Bb, bb 1 correct analysis of phenotypes i.e. 3 black fur 1 with brown fur 1 (ii) award one mark for the recognition that it is down to chance (which two gametes fuse) and not simply ‘because it’s a prediction’ do not accept mutation 1 (b) (i) B is dominant/ an allele is dominant if it is expressed in the heterozygous phenotype candidates are likely to use a variety of ways of expressing their ideas Page 42 1 b is recessive/ a recessive allele is not expressed in the presence of its contrasting allele do not accept powerful do not accept stronger 1 (ii) alleles are different forms of a gene controlling a characteristic and occupying the same site on homologous chromosomes (e.g. B or b) 1 genes are the units of DNA/sites on chromosomes carrying the information that determines characteristics (e.g. bB) 1 (c) homozygous: BB / bb / possessing a pair of identical alleles for a character/true breeding give credit to an explanation using a diagram 1 heterozygous: Bb / carrying a pair of contrasting/different alleles for a characteristic do not accept references to xx, xy do not accept gene by itself 1 [10] M8. (i) with exercise rate rises; accept between 1 – 2 minutes rate rises 1 (when exercise stops) rate falls slowly; accept gentle fall or steady fall for answers which just describe a rise then a fall allow one mark only as an alternative to the first two points 1 Page 43 rate does not return to normal or to starting or to resting rate accept rate returns to normal after five minutes or three minutes of rest or after recording ended 1 (ii) 86 (per minute); 1 (iii) plotting points; deduct one mark for each error to max of two if 68 wrongly plotted count as one error (ignore the quality of the line) 2 [6] M9. (a) (i) plotting values for pulse rates; 2 marks- minus 1 mark for each error to a maximum of 2 Accept values if plotted on blood volume bar chart Non-horizontal tops to bars producing variable values = 1 error If drawn as a line graph =1 mark maximum 2 Page 44 (ii) Either volume of blood went up then fell; Accept went to a maximum then fell pulse rate increased (steadily); Accept went up steadily or kept going up 2 Or at first or with low activity or with moderate activity both pulse and volume increased; Accept activity up to wall- papering with more activity pulse continued to increase but volume fell; (b) Any two of Page 45 with increased activity greater muscle use or greater respiration; need more glucose or oxygen; Accept more sugar heart beat faster; Do not accept more air Accept more blood needed or blood flows faster If ‘more’ or equivalent stated once it can be accepted elsewhere by implication 2 [6] M10. (a) reactants: CO2 + H2O 1 products: C6H12O6 + O2 1 balance: 6CO2 + 6H2O → C6H12O6 + 6O2 1 (b) 1 mark each for any of the following ideas: lower CO2 concentration lower light intensity decrease water availability alter light wavelength or colour accept more green light 2 (c) (i) scales correctly constructed i.e. equal intervals along each axis 1 points plotted correctly 1 Page 46 appropriate line correctly drawn accept dot to dot or line of best fit cancel if line extends through zero or beyond 50°C 1 (ii) 18 – 19 (bubbles per minute) 1 (iii) heat denatures enzymes or destroys membranes or ruptures cells or destroys cells do not accept kills enzymes 1 [10] M11. (a) award one mark for each key idea energy released or energy transferred or respiration allow provides or gives do not allow produces or makes 3 near to the site of movement or energy available quickly or more energy accept allows more mitochondria to fit in (mitochondria) packed (around filament) or efficient arrangement or spiral arrangement (b) contains chromosomes or genes or DNA not genetic material 1 Page 47 (which) contribute half (the genes) to the fetus or offspring 23 chromosomes or half the genes or reference to X,Y chromosome determining sex (if the notion of halfness is there) nucleus contains half genes for the offspring = 2 marks 1 [5] M12. any four from: more energy / respiration required accept it prevents / reduces anaerobic respiration or less / no lactic acid reference to increase must be made, but only needed once, provided inference is clear for remainder of points. accept ‘delivered more quickly’ for ‘increase’ increase oxygen uptake into blood (in lungs) increase oxygen delivery to muscles increase glucose delivery to muscles increase removal of heat from muscles or increase delivery of heat to skin increase removal of carbon dioxide from muscles increase removal of carbon dioxide from blood (in lungs) [4] M13. (a) (before exercise) – 9 to 11 and (after exercise) – 12 or 13 both correct 1 Page 48 (b) 0.75 to 0.90 ignore working or lack of working eg. 2.35 – 1.55 or for 1 mark or other suitable figures 2 (c) any four from: still need to remove extra carbon dioxide still need to remove heat / to cool (some) anaerobic respiration (in exercise) lactic acid made (in exercise) oxygen needed to break down lactic acid or suitable reference to oxygen debt lactic acid broken down to CO2 and water or lactic acid changed into glucose 4 [7] M14. (a) (i) Aa or aA 1 (ii) allele / gene for vestigial wings / a is recessive or vestigial is recessive or A is dominant or A would override the effect of a or A present gives long wings 1 (b) parental genotypes correct – both Aa NB can pick up chain of logic at any point correctly derived from candidate’s previous point 1 gametes correctly derived from P genotypes 1 offspring genotypes correctly derived from gametes 1 3:1 ratio recognised wrong cross and not 3:1 ratio = max 2 Page 49 1 [6] M15. (a) A = meiosis accept ‘mieosis’ do not accept ‘miosis’ 1 B = mitosis do not accept ‘meitosis’ etc 1 (b) fertilisation allow conception 1 (c) (i) 23 1 (ii) 46 1 [5] M16. (a) lipase 1 (b) fatty acid ignore glycerol 1 (c) (i) 0.25 or if correct answer ignore working or lack of working Page 50 for 1 mark 2 (ii) fats emulsified or described re. Small droplets or large S.A. (for enzyme action) or fats ‘mix’ better with water do not allow breakdown / breakup unqualified 1 [5] M17. follow diet A because it gives the highest proportion of stored sugar in the muscles for 1 mark each [2] M18. (a) any three from: • ((mean) mass) increases up to 7 / 8 units (of light) then levels off • light limiting factor up to 7 / 8 units • for photosynthesis must be in correct context • other factor / temperature limiting above 7 / 8 units 3 (b) any two from: • cost of providing conditions / heat / light / CO2 • effect of treatment on profit allow too much of factor is wasteful • relevant use of data from graph eg limiting factors Page 51 • named other factors eg fertiliser / pest control / weeds / density of planting allow taste / appearance 2 (c) nitrate function produce amino acids / proteins / enzymes ignore DNA do not allow chlorophyll 1 nitrate deficiency stunted growth allow description ignore plant dies 1 magnesium function produce chlorophyll ignore chloroplasts 1 magnesium deficiency yellow leaves / plant ignore plant dies 1 [9] M19. (a) the enzyme must be lipase 1 since fatty acid produced, which lowered the pH 1 (b) (i) 0.25 or correct answer with / without working Page 52 if answer incorrect / missing, then evidence of gains 1 mark 2 (ii) bile provides optimum / suitable / best pH for enzyme action therefore the rate of the reaction increased 1 [5] M20. (a) eggs produced by meiosis 1 therefore contain only half of mother’s DNA 1 (b) (man B) no marks (child has) mother’s 25 / 28 / 30 / 31 or child gets 17 / 19 / 22 / 24 from mother 1 (child has) man B’s 10 / 12 / 13 / 14 or child gets 18 / 20 / 21 / 23 from B 1 no bars / DNA / lines from man A correspond to child 1 [5] M21. (a) 1 in 4 / 1/4 / 1: 3 / 25% / 0.25 do not accept 3:1 / 1:4 / 2:6 Page 53 1 (b) either from C and D accept synonyms for dominant / recessive eg Normal / faulty accept genetic diagram if clearly referring to correct individuals or genotypes on family tree allow ‘gene’ for ‘allele’ any three from: • C and D have disorder ignore ‘C & D are carriers’ • I/J don’t have disorder • C and D have dominant and recessive alleles • recessive alleles from C and D passed to I/J or I/J have two recessive alleles NB if allele was recessive then all offspring of C and D would have the disorder = 3 marks or from A and B assume response refers to A + B unless contradicted • A is homozygous recessive / rr, and B is heterozygous / Rr can be shown in words or symbols allow any symbol • offspring can be rr or Rr described allow without key 3 (c) (i) (embryos) checked for inherited / genetic disorders / conditions accept diseases for disorders 1 (ii) any three from: • C/D have disorder / have dominant allele accept disease / condition accept ‘gene’ for ‘allele’ ignore reference to ‘carriers’ • chance of embryo / foetus / child having disorder or may pass on alleles for disorder to their offspring Page 54 • C/D might want to decide on termination or prepare for child with disorder • G and H don.t have disorder / both homozygous recessive / have no dominant alleles (for this disorder) • so offspring (of G and H) cannot / don.t have disorder 3 [8] M22. (a) any four from: • mutation / variation • produces smaller wings / fatter body must be linked to mutation / variation • wings no longer an advantage since no predators allow wings / flight not needed as no predators • wings no longer an advantage since food on ground allow wings / flight not needed as food on ground • fatter body can store more energy when fruit scarce • successful birds breed / pass on genes 4 (b) any one from: • evidence has all gone • no scientists on island at time to record evidence • no records (from sailors) 1 [5] Page 55 M23. (a) any two from: • neutralises acid / makes conditions alkaline / raises pH • enzymes (in small intestine) work (more/most effectively) or stop/prevents enzymes being denatured • emulsifies fats/lipids or description of emulsification do not accept breakdown unqualified • larger surface area 2 (b) (i) bile / bilirubin / pigment / broken down haemoglobin / substance / cholesterol linked to movement or effect 1 does not get to the intestine / food / faeces or cannot leave liver or effect not happening (in intestine) 1 (ii) bilirubin / pigment / broken down haemoglobin not ‘bile’ alone 1 (deposited) in skin only award if bilirubin / pigment / broken down haemoglobin given allow carried in the blood 1 [6] Page 56