STUDY PROGRAMME
DEPARTMENT
NAME OF SUBJECT
STATUS OF THE
SUBJECT
Integrated studies of dentistry
Department fo histology and embriology
Optional
Year of studies
Winter term (No.of the lessons per week
Lectures Exercises
2 1 second
Summer term (No.of the lessons per week)
Lectures Exercises
- -
No.of tests theory, practical exercises, self-development seminar work
1
No.of seminars seminara
1
No.of seminars
POINTS
6,0
That students learn techniques of microscopic histology preparations
Knowledg e
Supposition of laboratory work, the choice of fixative in working with biological materials, methods of processing biological materials with microscopic characteristics of particular expertise, including simple and complex method of staining, the tissue culture laboratory medicine, pathology laboratory animals, norms and the prevention of illness in working with laboratory animals.
Skills
Mastering work in the laboratory of biological materials with special emphasis on accident prevention, preparation solution in laboratory work, preparation of native and vital microscopic preparation, fixation and further processing of the different tissue samples (rinse, dehydration, inclusion, molding), the use microtomes, staining microscopic preparations , Working with laboratory animals, preparation and maintenance of tissue culture, mastering the technique of obduction of laboratory animals.
Theoretical teaching – methodical units
1. Microscopy, history, types of
2. Classification of toxins by WHO and the prevention of poisoning and drutih accident in the laboratory histološkoj
3. Methods of tissue fixation, choice of fixative for light and electronic microscopy
4. Blood and tissue smear and prints, cytological characteristics of some samples, bazofilija and eosinophilia as well as representatives cytological structure
5. Microtomes and work with them (history of Purkinjea, manual, rotary, slide, kriotom)
6. Classification histology color staining method, a simple staining
7. Complex staining
8. Selective coloring, the most important reactions citohemijske
9. Bacteriological methods of dyeing, simple and complex
10. Preparation of microscopic preparations helminata and artropoda
11. Tissue culture
12. Biology and conditions of work with laboratory animals
13. Pathology of laboratory animals and the prevention antropozoonoses
14. Consultation paper for the seminar and exam
15. Consultation paper for the seminar and exam
Practical teaching – methodical units
1. Native and vitally stained microscopic preparations
2. Measuring the technical balance; pipetiranje and preparing the solution; first aid for poisoning
3. Preparation of fixative, taking snippets of tissue, rinsing after fixation, dehydration
4. Dyeing method of Giemsa and Papanicolaou, leukocitna formula in laboratory animals
5. Molding in paraffin for histological classical technique, cutting the preparations microtomes
6. Staining toluidinom, lipstick, determining phase estrusnog cycle in rodents
7. Hematoxylin staining and eozinom, van Gieson
8. Peroxidase, iron and others.
9. Gram staining and objectives Nilzenu
10. Methods of illumination Metazoa laktofenolom et al.
11. Eksplantacija, primary and continuous culture
12. Experimental animal models of disease
13. Methods obdukcioni diagnostic laboratory animals
14. Writing a seminar paper
15. Pre-exam Exercises
RECOMMANDED
READING
Compulsor y
1.
Lalošević D. Mikroskopska laboratorijska tehnika u medicini. Medicinski fakultet Novi Sad,
2005.

Lectures
Additional
1.Kostić A. Osnovi histološke tehnike. Naučna knjiga, Beograd, 1948.
2.
Bancroft J.D, Stevens A. Theory and practice of histological techniques. Churchill
Livingstone, Edinburgh, 2005.
Exercises
Pre-exam obligations Final exam
Test
Seminar paper
The rest Pismeni Oral
Professor PhD
3
Associate prof.
1
Professor Scientist Associate Assistent Lecturer
1 1
1.Prof dr Dušan Lalošević
2.Doc. dr Matilda Đolai
3.Doc. dr Dušan Božić
4.Doc. dr Aleksandar Savić
5. Dr Nenad Šolajić, sar. u nastavi
6. Asst.mr Ivana Milošević