Resolution and Pupil size Is the resolving power of the eye affected by the size of the pupil? Introduction Resolution is the ability to see fine detail in an image; it can be quantified by separation of two distinguishable points. According to text books at this level (Giancoli, 1995) the resolution an optical instrument is dependent on the size of the aperture; this is one of the reasons why high resolution telescopes have such large diameter mirrors (Hamper, 2009). The eye is an optical instrument that has a small aperture (the pupil) according to the same principle its resolution should therefore be dependent on the pupil size which would imply that our eyes could resolve more detail in the dark when the pupil is large than in bright light when it is small, this seems strange so in this essay the question “Is the resolving power of the eye affected by the size of the pupil?” will be addressed. Diffraction by small apertures When light passes through a small aperture it spreads out to produce an intensity pattern shown in fig.1, this is called diffraction and can be explained using Huygens’ construction. This models the wave front as an infinite number of small wavelet sources each propagating a spherical wave in the forward direction, these wavelets can be summed to give the resultant intensity at any given angle. Using this model it can be shown that the angular separation of the first minima θ = 1.22λ/a where a is the diameter of the aperture. If an image of a point object is produced by an optical instrument diffraction at the aperture will cause the image to be a series of rings rather than a point. Fig. 1 Diffraction of light by a circular aperture Rayleigh Criterion The Rayleigh criterion is a way of defining the resolving power of an optical instrument, this states that “two images are just resolvable when the centre of the diffraction disk of one is directly over the first minimum of the other” (Giancoli, 1995 p.727). This is illustrated in fig.2. The outcome of this is that the resolving power of an optical instrument is dependent on the size of the aperture. Fig. 2 Rayleigh Criterion The Human Eye Light enters the eye (fig.3) through the pupil and is focused on the retina by the lens. The retina is made up of millions of light sensitive cells that convert the light to a nerve impulse that can be sent to the brain via the optic nerve. The cells of the retina do not work well if the light is either too bright or too dark; to optimise the amount of light entering the eye the iris controls the size of the pupil making it smaller when the light is bright and bigger when the light is dark. The average size of the pupil can vary from 3-9mm (Wikepedia, 2010). Since the amount of light entering the eye is proportional to the diameter squared the pupil can vary the light by a factor of 9, this is not enough to deal with the difference from seeing in bright sunlight to seeing stars at night a factor 107 (Clark R.N. 2005), this is managed by chemical changes that take place in light sensitive cells themselves combined with using different cells for low light conditions. The resolving power of the eye The resolving power can be quantified by the angle subtended between the two closest points that can be seen apart. Using the Rayleigh criterion it is possible to calculate a value for the resolving power of the eye. If the wavelength of light is 5nm and the diameter of the pupil is 5mm the using the equation θ = 1.22λ/a we can deduce that the points will not be resolved if the angle between them is less than 1.2x 10-4 radians. This means that if two objects 1mm apart will be able to be distinguished at a distance of 1/1.2x10-4 mm, approximately 8m. Fig. 4 Angle subtended by two point objects Fig. 3 The Human eye Measuring the Resolving Power of the Eye To measure the resolving power of the eye two close together objects are viewed close to the eye, the distance between the eye and the objects is then increased until they can no longer be seen as two separate lines. A commonly used variation of this (Stokes H.T.) is to use a series of lines and decide at what distance they appear to be continuous. Fig.5 shows an example of this. As the page is moved from the eye, at some distance x the lines appear to be the same as the filled square. If the separation of the lines is d then the resolving power is d/x radians. A trial of this with a line separation of 0.78mm revealed that the lines became indistinguishable at a distance of 185 ±2 cm this gives a resolving power of about 4 x 10-4 rads which is not as good as that predicted by the Rayleigh criterion. Measuring the size of the pupil Since the experiment was to be performed alone the easiest way to measure the pupil size was by using a camera. This was set up at a fixed distance from the eye and a picture recorded. The picture was then uploaded to my computer and the programme LoggerPro (Vernier) was used to measure the pupil size. To scale the measurements a mm scale was placed close to the eye. Fig.6 is an example of one of the photographs. Fig. 6 Measuring the eye In this photograph the scale was set using the ruler on the left, the eye was then measured at several places and found to have an average diameter of 0.49 ± 0.03cm. To make the pupil change size the light intensity had to be changed, this caused problems when photographing the eye, it was also very difficult to carry out the resolution test and measure the eye at the same time for this reason it was decided not to measure the eye pupil but to carry out the test at different light intensity with the assumption that the pupil size will increase with decreasing light intensity. Fig. 5 The resolving power test Measuring resolving Power with different pupil sizes To test the resolving power of the eye a grid similar to fig.5 was printed onto white paper and mounted on a wall. To change the pupil size the light intensity needed to be varied but doing that would make the room dark therefore making the lines, more difficult to see so the challenge was to increase the size of the pupil without making the room dark. The solution used was to place a black tube in front of the eye; this would reduce the amount of light incident on the eye without making the object appear dark. By increasing the length of the tube the light falling on the eye would become less and the pupil bigger. The way this works can be illustrated in fig.7. Where the observer is viewing light from an illuminated wall, light at a wider angle than that shown cannot pass down the tube into the eye so as the tube length is increased so the amount of light entering the eye is reduced. Fig. 7 varying light intensity with a tube To test the effectiveness of this system a light sensor was placed at the end of the tube as its length was increased; the results are shown in graph 1. Preliminary trials of the experiment highlighted a problem regarding my eyesight. I am longsighted which means I have to wear glasses to read, without glasses I cannot focus on objects closer than about 3m and beyond that I can’t focus with glasses. For that reason The grid used was big enough to be resolved from a great enough distance so that I did not have to use glasses.