OIE Reference Laboratory Reports
Activities in 2012
Name of disease (or topic) for which you
are a designated OIE Reference
Laboratory:
Address of laboratory
Avian Influenza
Australian Animal Health Laboratory
CSIRO Livestock Industries
Private Bag 24
Geelong VIC 3220
AUSTRALIA
Tel.:
+61 3 5227 5000
Fax:
+61 3 5227 5555
e-mail address:
peter.daniels@csiro.au
website:
www.csiro.au
Name (including Title) of Head of
Laboratory (Responsible Official):
Professor Martyn Jeggo
Name (including Title and Position) of OIE
Reference Expert:
Paul Selleck (retired)
Date of submission to the OIE
27 January 2013
Annual reports of OIE Reference Centres, 2012
1
OIE RL for « Avian Influenza » – « Paul Selleck » – « Australia »
ToR: To use, promote and disseminate diagnostic methods validated according to OIE
Standards
Test recommended by the OIE
Indirect diagnostic tests
C-ELISA – Flu A group
C-ELISA - N1
Total number of test performed last year
Nationally
Internationally
2098
468
12
0
AGID - Flu A group
0
H5 HI test
2542
468
H7 HI test
165
0
H9 HI test
258
0
HI test (other)
0
VNT
0
Direct Diagnostic tests
Nationally
Internationally
HA typing
5
0
NA typing
10
0
Taqman real-time PCR – Flu A Group
670
60
Taqman real-time PCR – H5
292
60
Taqman real-time PCR – H7
125
5
Taqman real-time PCR – H9
58
4
Taqman real-time PCR – N1
31
60
PCR and sequencing - HA gene cleavage
site
28
PCR and sequencing - HA/NA subtyping
45
60
PCR and sequencing - NA Sequencing
40
60
PCR and sequencing - HA Sequencing
49
60
Embryonated chicken egg culture
570
60
5
0
Avian influenza immunohistochemistry
60
ToR: To develop reference material in accordance with OIE requirements, and implement
and promote the application of OIE Standards.
To store and distribute to national laboratories biological reference products and any
other reagents used in the diagnosis and control of the designated pathogens or
disease.
2.
Did your laboratory produce or store imported standard reference reagents officially recognised by
the OIE or other international bodies?
Yes
2
No
Annual reports of OIE Reference Centres, 2012
OIE RL for « Avian Influenza » – « Paul Selleck » – « Australia »
3.
Did your laboratory supply standard reference reagents to OIE Member Countries?
Yes
No
Amount
supplied
nationally
(ml, mg)
Amount supplied
internationally
(ml, mg)
HI
95 ml
338 ml
Antisera to
A/chicken/Koma
we
Selatan/BBVM20
4 (0)/07
HI
33 ml
12 ml
AI reference
antisera
AI HI
Type of reagent
available
Antigen for the
H5 HI test
Related
diagnostic
test
Produced/
stored
A/chicken/Koma
weSelatan/BBVM
204 (0)/07
Name of
recipient OIE
Member
Countries and
of institutions
Cambodia,
China,
Indonesia, Lao
PDR, Myanmar,
Malaysia,
Philippines,
Thailand,
Vietnam, and
Timor leste
“
-
One ml each of:
Indonesia
A/ck/West
Java/CSKLEB/06 H5N1
A/ck/West
Java/CSKLEB/2006 H5N1
A/mallard/South
Korea/12A/06 H5N2
A/avian/669/WA/78
H3N8
A/chicken/Konawe
Selatan/BBVM204/07 H5N1
A/chicken/Konawe
Selatan/BBVM204/2007 H5N1 (ex
bird 11)
A/shearwater/Austra
lia/1975 H5N3
A/mallard/South
Korea/12A/06 H5N2
AI C-ELISA
reagent:
AI C-ELISA
3 ml
-
-
AI C-ELISA
5.5 ml
15 ml
Indonesia
Network QC
AI C-ELISA
reagent:
Positive Control
serum
Annual reports of OIE Reference Laboratories, 2012
3
OIE RL for « Avian Influenza » – « Paul Selleck » – « Australia »
Type of reagent
available
Antigen coated
ELISA plates
AI C-ELISA
reagent:
Name of
recipient OIE
Member
Countries and
of institutions
Related
diagnostic
test
Produced/
stored
Amount
supplied
nationally
(ml, mg)
AI C-ELISA
200
35
100 (sufficient for
5000-6000 tests)
Indonesia
AI C-ELISA
4.ml
12 ml
Indonesia
AI Matrix
protein
qPCR
50 ml
-
-
4.5 ml
Cambodia,
China,
Indonesia, Lao
PDR, Myanmar,
Malaysia,
Philippines,
Thailand,
Vietnam
4.5 ml
Cambodia,
China,
Indonesia, Lao
PDR, Myanmar,
Malaysia,
Philippines,
Thailand,
Vietnam
Amount supplied
internationally
(ml, mg)
Mab to the NP
Network Quality
Control
AI Matrix gene
qPCR positive
control
A/chicken/Myanma
r/295/2010
AI H5 HA gene qPCR
positive control
A/chicken/Laos/Xay
thani /26/2006
4.
AI Matrix
gene qPCR
AI H5 HA
gene qPCR
Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference
reagents?
x Yes
5.
No
Did your laboratory produce vaccines?
Yes
6.
No
Did your laboratory supply vaccines to OIE Member Countries?
Yes
No
ToR: To develop, standardise and validate, according to OIE Standards, new procedures for
diagnosis and control of the designated pathogens or diseases
7.
Did your laboratory develop new diagnostic methods validated according to OIE Standards for the
designated pathogen or disease?
Yes
8.
Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen
or disease?
Yes
4
No
No
Annual reports of OIE Reference Centres, 2012
OIE RL for « Avian Influenza » – « Paul Selleck » – « Australia »
ToR: To provide diagnostic testing facilities, and, where appropriate, scientific and
technical advice on disease control measures to OIE Member Countries
9.
Did your laboratory carry out diagnostic testing for other OIE Member Countries?
Yes
No
Name of OIE
Member Country
seeking assistance
Date
(dd/mm)
No. samples received for
provision of diagnostic support
(i.e. from surveillance campaign)
No. samples received for
provision of confirmatory
diagnoses
Nepal
5 April
0
10
Myanmar
4 May
0
10
Indonesia
17 May
0
36
Papua New Guinea
7 March,
30 April, 4 June,
3 August,
23 November
434
0
East Timor
19 November
53
0
0
4
New Zealand
10.
Did your laboratory provide expert advice in technical consultancies on the request of an OIE
Member Country?
Yes
No
Name of the OIE Member Country
receiving a technical consultancy
High Security Animal Disease
Laboratory, Indian Veterinary
Research Institute, Indian Council
of Agricultural Research, Bhopal,
India
How the advice was
provided
Purpose
Advise on laboratory management
capacities particularly in biosafety
equipment maintenance for work on
pathogens such as HPAI
On site consultations
ToR: To carry out and/or coordinate scientific and technical studies in collaboration with
other laboratories, centres or organisations
11.
Did your laboratory participate in international scientific studies in collaboration with OIE Member
Countries other than the own?
Yes
No
Title of the study
Duration
Purpose of the study
Facilitation and
implementation
of laboratory
training and
studies on
samples
submitted as
isolates of avian
2 years
Antigenic mapping
and molecular
epidemiology studies
of H5N1 HPAI and
capacity building
Annual reports of OIE Reference Laboratories, 2012
Partners
(Institutions)
Indonesia
Directorate of
Animal Health
OIE Member Countries
involved other than
your country
Indonesia
5
OIE RL for « Avian Influenza » – « Paul Selleck » – « Australia »
influenza viruses
from Indonesia
Surveillance tools
and strategies for
improved control,
monitoring and
eradication of
avian influenza in
Indonesia
3 years
Field studies for
monitoring of HPAI in
vaccinated flocks,
and development of
technological tools
Research Institute
for Veterinary
Science (Bbalitvet)
Indonesia
A project to
improve the
yields of HPAI
vaccines grown in
eggs
1 year
to improve the yields
of HPAI vaccines
grown in eggs
University of
Georgia
USA
Development of
poultry that will
be substantially
resistant to AI
infection
5 years
To use RNAi and
transgenetics to
develop poultry
substantially
resistant to AI
infection
Korea, UK
ToR: To collect, process, analyse, publish and disseminate epizootiological data relevant to
the designated pathogens or diseases
12.
Did your Laboratory collect epizootiological data relevant to international disease control?
Yes
13.
No
Did your laboratory disseminate epizootiological data that had been processed and analysed?
Yes
14.
No
What method of dissemination of information is most often used by your laboratory?
(Indicate in the appropriate box the number by category)
a)
Articles published in peer-reviewed journals: ................. 21
b)
International conferences:9
c)
National conferences: ...................................................... 10
d)
Other: ...............................................................................
(See list of publications attached)
ToR: To provide scientific and technical training for personnel from OIE Member Countries
To recommend the prescribed and alternative tests of vaccines as OIE Standards
15.
Did your laboratory provide scientific and technical training to laboratory personnel from other OIE
Member Countries?
Yes
6
No
a)
Technical visits: ................................................................ 6
b)
Seminars: ......................................................................... NA
Annual reports of OIE Reference Centres, 2012
OIE RL for « Avian Influenza » – « Paul Selleck » – « Australia »
c)
Hands-on training courses: .............................................. 1
d)
Internships (>1 month): ................................................... NA
Type of technical training
provided (a, b, c or d)
Country of origin of the expert(s)
provided with training
No. participants from the
corresponding country
a
In Indonesia (several locations)
12
a
In India
8
a
In Thailand, for trainees from Myanmar,
Cambodia, Malaysia, Vietnam,
Philippines and Thailand
20
a
In Indonesia (several locations)
40
a
Visits to Myanmar, Cambodia, Malaysia,
Vietnam, Philippines and Thailand
60
a
In Indonesia
20
c
At AAHL, from 8 Southeast Asia regional
countries
18
ToR: To maintain a system of quality assurance, biosafety and biosecurity relevant for the
pathogen and the disease concerned
16.
Does your laboratory have a Quality Management System certified according to an International
Standard?
Yes
No
AAHL maintains certification to AS/NZS ISO 9001:2008 for the management of its Quality
Assurance System as well as AS/NZS ISO 14001:2004 for environmental management.
17.
Is your laboratory accredited by an international accreditation body?
Yes
No
AAHL’s diagnostic operations are conducted under NATA accreditation to ISO/IEC
17025:2005. AAHL is also accredited to ISO/IEC 17043:2010 as an international proficiency
testing provider for exotic disease agents.
Test for which your laboratory is accredited
20.10 Microbiology
For companion animals, production animals, production avian species, aquatic
animals, equine species and avian species
Accreditation body
NATA, affiliated
with ILAC
20.11 Bacteriology
.01 Diagnostic bacteriology - incorporating identification by simple microscopy,
cultural methods of detection and identification of organisms
.03 Immunological methods of antigen detection
20.13 Other Microorganisms (including mycoplasma, rickettsia, algae)
.01 Diagnostic microbiology - incorporating identification by simple microscopy,
cultural methods of detection and identification of organisms, including inocuity
testing
20.14 Virology
.01 Diagnostic virology - non-cultural (immunological) methods of detection
Annual reports of OIE Reference Laboratories, 2012
7
OIE RL for « Avian Influenza » – « Paul Selleck » – « Australia »
.02 Diagnostic virology - cultural methods of detection and identification of
organisms, including inocuity testing
.05 Quantitative procedures
20.15 Prions
.01 Histological identification of prion disease lesions
.02 Detection of prion protein by immunological methods (including ELISA, Western
Blots, immunohistochemistry)
.04 Detection of prion protein by bioassay
20.25 Serology of Infection
For companion animals, production animals, production avian species, equine
species and avian species
.01 Agar gel immunodiffusion tests
.02 Complement fixation tests
.03 Enzyme linked immunosorbent assays
.04 Haemagglutination inhibition
.05 Indirect fluorescent antibody tests
.06 Microscopic agglutination tests
.08 Serum agglutination tests
.09 Serum neutralisation tests
.10 Latex agglutination tests
.99 Other : Testing for rabies and rabies related lyssaviruses on human specimens
20.50 Anatomical Pathology
For companion animals, production animals, production avian species, laboratory
animals, zoo animals, wildlife, aquatic animals, equine species and avian species
20.52 Histopathology
.01 Processing of fixed specimens for histology
.04 Immunohistochemistry
.05 Histological interpretation
20.53 Electron Microscopy
.01 Transmission electron microscopy
.02 Scanning electron microscopy
.04 Immunohistochemistry electron microscopy
20.54 Necropsy
20.80 Molecular Diagnostics
.01 Identification by extraction and amplification
.02 Sequencing
.03 Genotyping
.99 Other : Testing for rabies and rabies related lyssaviruses on human specimens by
molecular techniques
18. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease
concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 or
Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1)
Yes
No
ToR: To organise and participate in scientific meetings on behalf of the OIE
19.
Did your laboratory organise scientific meetings on behalf of the OIE?
Yes
8
No
Annual reports of OIE Reference Centres, 2012
OIE RL for « Avian Influenza » – « Paul Selleck » – « Australia »
20.
Did your laboratory participate in scientific meetings on behalf of the OIE?
Yes
Title of event
The 7th Asia-Pacific
Biosafety Association
Scientific Conference,
Moving Towards One
World-One Health,
No
Date
(mm/yy)
Location
Role (speaker,
presenting poster,
short
communications)
Title of the work
presented
04/12
Bali,
Indonesia.
Speaker
Emerging and Reemerging Disease
Control. Facing New
Challenges: Emerging
Pathogens & Disease
Surveillance
ToR: To establish and maintain a network with other OIE Reference Laboratories
designated for the same pathogen or disease and organise regular inter-laboratory
proficiency testing to ensure comparability of results
21.
Did your laboratory exchange information with other OIE Reference Laboratories designated for the
same pathogen or disease?
Yes
22.
No
Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated
for the same pathogen or disease by organising or participating in proficiency tests?
Yes
No
Purpose of the proficiency tests:
(validation of a diagnostic protocol:
specify the test; quality control of
vaccines: specify the vaccine type, etc.)
Role of your Reference
Laboratory (organiser/
participant)
OFFLU organized ring trial for real time
PCR tests for Flu A, H5 and H7 genes,
reporting and follow up on a panel
distributed the previous year
Participant
23.
No.
particip
ants
Participating OIE Ref.
Labs/ organising OIE
Ref. Lab.
National Veterinary
Services Laboratories
USDA, APHIS,
P.O. Box 844 Ames,
Iowa 50010 USA
Did your laboratory collaborate with other OIE Reference Laboratories for the same disease
on scientific research projects for the diagnosis or control of the pathogen of interest?
Yes
No
ToR: To organise inter-laboratory proficiency testing with laboratories other than OIE
Reference Laboratories for the same pathogens and diseases to ensure equivalence of
results.
24.
Did your laboratory organise or participate in inter-laboratory proficiency tests with
laboratories other than OIE Reference Laboratories for the same disease?
Yes
Annual reports of OIE Reference Laboratories, 2012
No
9
OIE RL for « Avian Influenza » – « Paul Selleck » – « Australia »
Purpose for inter-laboratory test comparisons1
No. participating
laboratories
Participating OIE Member
Countries
Test laboratory sensitivity and specificity for
influenza A viruses and also the H5 gene by real
time PCR
10
Cambodia, China, Indonesia, Lao
PDR, Myanmar, Malaysia,
Philippines, Thailand, Vietnam
Test laboratory sensitivity and specificity for
detection of antibody to influenza A H5 by the
haemagglutination inhibition test
11
Cambodia, China, Indonesia, Lao
PDR, Myanmar, Malaysia,
Philippines, Thailand, Vietnam, and
Timor Leste
Test laboratory sensitivity and specificity for
influenza A viruses (matrix gene) by real time
PCR
11
Australia (State government
laboratories in the national
laboratory network)
Test laboratory sensitivity and specificity for
detection of antibody to influenza A viruses in
chickens by the C-ELISA test
6
Australia (State government
laboratories in the national
laboratory network)
ToR: To place expert consultants at the disposal of the OIE
25.
Did your laboratory place expert consultants at the disposal of the OIE?
Yes
No
Kind of consultancy
Location
Subject (facultative )
A scoping mission
Bangladesh
To create a workshop on
Laboratory Networking and
Proficiency Testing for priority
HPEDs in SAARC countries January
2012
Meeting participation as an OIE
representative
Vietnam and
Indonesia
4 Way Linking meeting: animal
health-public health, laboratory
and field epidemiology, February
and December, 2012
Meeting participation for OFFLU
Paris
The 2nd OFFLU Swine Influenza
Technical Activity Meeting, March
2012
Meeting participation
Thailand
An OIE/FAO meeting on HPAI, July
2012
A scoping mission
Thailand
For the Laboratory-Field
Epidemiology Linkage Training July
2012
OIE Twinning project in avian influenza and
Newcastle Disease
The Veterinary
Research Institute,
Ipoh, Malaysia
5 visits throughout the year, on
project oversight, diagnostic
virology, biorisk engineering,
quality assurance system and
1
10
See Interlaboratory test comparisons in: Laboratory Proficiency Testing at:
www.oie.int/en/our-scientific-expertise/reference-laboratories/proficiency-testing see point 1.3
Annual reports of OIE Reference Centres, 2012
OIE RL for « Avian Influenza » – « Paul Selleck » – « Australia »
management (2)
OIE Training Workshop
Thailand
For OIE National Focal Points on
Laboratories, August 2012
Meeting participation
Vietnam
The Fourth Laboratory Directors
Forum Meeting (SE Asian region),
October 2012
Meeting participation
Vienna
Expert consultation for establishing
and maintaining core facilities for
molecular test diagnosis in
veterinary laboratories of
developing countries with limited
resources, October 2012.
Meeting participation for OFFLU
Rome
Workshop on Influenza Risk
Assessment and Risk Modelling:
Pandemic Influenza Threats and
Beyond November 2012
Ad hoc group participation
Paris
Biosafety and Biosecurity in
Veterinary Laboratories, February
and July, 2012
Ad hoc group participation
Paris
New Approaches to Diagnosis:
Applied Genomics, December 2012
Member of the OIE Biological Standards
Commission
Paris
Particpated in the BSC meeting
September 2012
Publications
Journal Articles
 Arzey, G. G., Kirkland, P. D., Arzey, K. E., Frost, M., Maywood, P., Conaty, S., Selleck, P.
(2012). Influenza Virus A (H10N7) in Chickens and Poultry Abattoir Workers, Australia. Emerg
Infect Dis, 18(5), 814-816. doi: 10.3201/eid1805.111852
 Daniels, P., Wiyono, A., Sawitri, E., Poermadjaja, B., & Sims, L. (2012). H5N1 highly
pathogenic avian influenza in Indonesia: retrospective considerations. Curr Top Microbiol
Immunol, online in press, 20.
 Deng, Y.-M., Iannello, P., Smith, I., Watson, J., Barr, I. G., Daniels, P., Wong, F. Y. K. (2012).
Transmission of influenza A(H1N1) 2009 pandemic viruses in Australian swine. Influenza and
Other Respiratory Viruses, 6(3), e42-e47. doi: 10.1111/j.1750-2659.2012.00337.x
 El-Hage, C. M., Savage, C. J., Minke, J. M., Ficorilli, N. P., Watson, J., & Gilkerson, J. R.
(2012). Accelerated vaccination schedule provides protective levels of antibody and complete
herd immunity to equine influenza. Equine Vet J. doi: 10.1111/j.2042-3306.2012.00605.x
 Henning, J., Wibawa, H., Yulianto, D., Usman, T. B., Junaidi, A., & Meers, J. (2012). The
management of smallholder duck flocks in Central Java, Indonesia, and potential hazards
promoting the spread of Highly Pathogenic Avian Influenza virus. Worlds Poultry Science
Journal, 68(3), 513-528. doi: 10.1017/s004393391200061x
 Hoque, M. A., Skerratt, L. F., Garland, S., Burgess, G. W., & Selleck, P. (2012). Adaptations of a
Competitive Enzyme-Linked Immunosorbent Assays for the Detection of Antibodies to Influenza
A Virus in Horse Sera for Use in Wild Aquatic Birds. Indian Journal of Virology, 23(3), 261-269.
doi: 10.1007/s13337-012-0074-3
 Hsu, A. C. Y., Parsons, K., Barr, I., Lowther, S., Middleton, D., Hansbro, P. M., & Wark, P. A.
B. (2012). Critical Role of Constitutive Type I Interferon Response in Bronchial Epithelial Cell to
Influenza Infection. PLoS One, 7(3). doi: 10.1371/journal.pone.0032947
 Jeggo, M., Butler, C., Jing, F., Weinstein, P., & Daszak, P. (2012). EcoHealth and the Influenza
A/H5N1 Dual Use Issue. Ecohealth, 9(1), 1-3. doi: 10.1007/s10393-012-0768-4
 Karpala, A. J., Lowenthal, J. W., & Bean, A. G. D. (2012). Identifying innate immune pathways
of the chicken may lead to new antiviral therapies. Vet Immunol Immunopathol, 148(1-2), 100109. doi: 10.1016/j.vetimm.2011.05.031
Annual reports of OIE Reference Laboratories, 2012
11
OIE RL for « Avian Influenza » – « Paul Selleck » – « Australia »












Lynch, G. W., Selleck, P., Church, W. B., & Sullivan, J. S. (2012). Seasoned adaptive antibody
immunity for highly pathogenic pandemic influenza in humans. Immunology and Cell Biology,
90(2), 149-158. doi: 10.1038/icb.2011.38
McKimm-Breschkin, J. L., Rootes, C., Mohr, P. G., Barrett, S., & Streltsov, V. A. (2012). In vitro
passaging of a pandemic H1N1/09 virus selects for viruses with neuraminidase mutations
conferring high-level resistance to oseltamivir and peramivir, but not to zanamivir. Journal of
Antimicrobial Chemotherapy, 67(8), 1874-1883. doi: 10.1093/jac/dks150
Meliopoulos, V. A., Andersen, L. E., Birrer, K. F., Simpson, K. J., Lowenthal, J. W., Bean, A. G.
D., Tripp, R. A. (2012). Host gene targets for novel influenza therapies elucidated by highthroughput RNA interference screens. Faseb Journal, 26(4), 1372-1386. doi: 10.1096/fj.11193466
Rockman, S., Maher, D., & Middleton, D. (2012). The use of hyperimmune serum for severe
influenza
infections.
Critical
Care
Medicine,
40(3),
973-975.
doi:
10.1097/CCM.0b013e318236f486
Rockman, S., Middleton, D. J., Pearse, M. J., Barr, I. G., Lowther, S., & Brown, L. E. (2012).
Control of pandemic (H1N1) 2009 influenza virus infection of ferret lungs by non-adjuvantcontaining pandemic and seasonal vaccines. Vaccine, 30(24), 3618-3623. doi:
10.1016/j.vaccine.2012.03.043
Schat, K. A., Bingham, J., Butler, J. M., Chen, L.-M., Lowther, S., Crowley, T. M., Lowenthal, J.
W. (2012). Role of Position 627 of PB2 and the Multibasic Cleavage Site of the Hemagglutinin in
the Virulence of H5N1 Avian Influenza Virus in Chickens and Ducks. PLoS One, 7(2). doi:
10.1371/journal.pone.0030960
Serrao, E., Meers, J., Pym, R., Copland, R., Eagles, D., & Henning, J. (2012). Prevalence and
incidence of Newcastle disease and prevalence of Avian Influenza infection of scavenging village
chickens in Timor-Leste. Preventive Veterinary Medicine, 104(3-4), 301-308. doi:
10.1016/j.prevetmed.2011.12.018
Stewart, C. R., Bagnaud-Baule, A., Karpala, A. J., Lowther, S., Mohr, P. G., Wise, T. G., Bean,
A. G. (2012). Toll-Like Receptor 7 Ligands Inhibit Influenza A Infection in Chickens. Journal of
Interferon and Cytokine Research, 32(1), 46-51. doi: 10.1089/jir.2011.0036
Verity, E. E., Camuglia, S., Agius, C. T., Ong, C., Shaw, R., Barr, I., Rockman, S. (2012). Rapid
generation of pandemic influenza virus vaccine candidate strains using synthetic DNA. Influenza
and Other Respiratory Viruses, 6(2), 101-109. doi: 10.1111/j.1750-2659.2011.00273.x
Vlahos, R., Stambas, J., & Selemidis, S. (2012). Suppressing production of reactive oxygen
species (ROS) for influenza A virus therapy. Trends in Pharmacological Sciences, 33(1), 3-8.
doi: 10.1016/j.tips.2011.09.001
Wibawa, H., Henning, J., Waluyati, D. E., Usman, T. B., Lowther, S., Bingham, J., Meers, J.
(2012). Comparison of serological assays for detecting antibodies in ducks exposed to H5
subtype avian influenza virus. BMC Veterinary Research, 8(117), (23 July 2012)-(2023 July
2012).
Yamada, M., Bingham, J., Payne, J., Rookes, J., Lowther, S., Haining, J., Middleton, D. (2012).
Multiple routes of invasion of wild-type Clade 1 highly pathogenic avian influenza H5N1 virus
into the central nervous system (CNS) after intranasal exposure in ferrets. Acta
Neuropathologica, 124(4), 505-516. doi: 10.1007/s00401-012-1010-8
Conference Papers
 Challagulla, A., Stewart, C., Guerrero-Sanchez, C., Grusche, F., Shi, S., Tizard, M., Hinton, T.
(2012). Inhibition of Influenza virus in chicken embryos by a RAFT designed ABA tri-block
copolymer delivered siRNA. Paper presented at the Sydney International Nanomedicine
Conference, Coogee Beach, Sydney.
 Crameri, S., Holmes, C., Leis, A., Smith, I., Shan, s., Lehmann, D., Hyatt, A. (2012). A novel
orthomyxovirus in Muscovy ducks electron microscopy reveals structural resemblance to
arenaviruses. Paper presented at the APMC10/ICONN2012/ACMM22 joint meeting, Perth.
 Daniels, P. (2012). Emerging and Re-emerging Disease Control Facing New Challenges:
Emerging Pathogens & Disease Surveillance Control (Abstract). Paper presented at the The 7th
APBA Scientific Conference, Moving Towards One World-One Health, Bali, Indonesia.
 Daniels, P. (2012). Animal Biosecurity, “One Health” and the Australian Animal Health
Laboratory, WEHI Postgraduate Lecture Series, 9 July 2012, Walter and Eliza Hall Institute,
Parkville, Victoria
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Annual reports of OIE Reference Centres, 2012
OIE RL for « Avian Influenza » – « Paul Selleck » – « Australia »
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Daniels, P. (2012). The Challenge of Influenza Surveillance in the Intensive Animal Industries,
The Joint NHMRC and Bill & Melinda Gates Foundation Forum: H5N1: are we prepared?, 1
November 2012, NHMRC Canberra
Daniels, P., Wong, F., Deng, Y.-M., Watson, J., Selleck, P., & Barr, I. (2012). Influenza A viral
infections in farm animals. Recent trends with an emphasis on Australian experiences. Paper
presented at the The 8th Australian Influenza Symposium, John Curtin School of Medical
Research, ANU, Canberra.
Nining Hartaningsih, Mia Kim, Paul Selleck, Frank Wong, James McGrane, Elly Sawitri,
Pudjiatmoko, Muhammed Azhar (2012) Sustainable approach to influenza virus monitoring for
animal health in Indonesia. 8th International Symposium on Avian Influenza, Royal Holloway,
University of London. 1-4 April 2012.
Jenkins, K., Doran, T., & Tizard, M. (2012). Control of Highly Pathogenic Avian Influenza:
Application of a Drosha parallel processing cassette in an approach for disease resistance
through transgenic delivery of anti-flu RNAi in the chicken. Paper presented at the Infection and
Immunity, Lorne, Vic.
Lowenthal, J., Stewart, C., Bean, A., Karpala, A., & Lowther, S. (2012). Immunostimulatory
siRNAs targeting H5N1avian influenza. Paper presented at the Avian Immunology Research
Group meeting, Edinburgh, UK.
Jennifer McKimm-Breschkin, Susan Barrett, Pudjiatmoko, Muhammad Azhar, Peter Mohr, Frank
Wong, Paul Selleck, Kerri Bruce, Julie Cooke, Mia Kim and James McGrane (2012) Screening
neuraminidase inhibitor susceptibility of avian influenza isolates fro SE Asia 2005-2009
identifies H5N1 I222 mutants with reduced oseltamivir sensitivity. 8 th International Symposium
on Avian Influenza, Royal Holloway, University of London. 1-4 April 2012.
Jenny McKimm-Breschkin, Susan Barrett, Pudjiatmoko, Muhammad Azhar, Peter Mohr, Frank
Wong, Paul Selleck, Kerri Bruce, Julie Cooke, Mia Kim and James McGrane (2012) Screening
neuraminidase inhibitor susceptibility of avian influenza H5N1 isolates from Indonesia from
2005-2008 identifies I222 mutants with reduced oseltamavir sensitivity. Emerging Infectious
Diseases Symposium, October 22-23, 2012, Geelong, Australia.
Paul Selleck, Tiggy Grillo, Frank Wong (2012) Avian Influenza in Australia 2008 – 2012. 8th
International Symposium on Avian Influenza, Royal Holloway, University of London. 1-4 April
2012.
Paul Selleck, Frank Wong, Mia Kim, Nining Hartaningsih, Elly Sawitri, Pudjiatmoko,
Muhammad Azhar, Gwenaelle Dauphin, James McGrane, John Allen and Peter Daniels (2012)
Monitoring antigenic variation of H5N1 avian influenza viruses in Indonesia towards improved
vaccine selection. 6th International Veterinary Vaccines and Diagnostics Conference, 29 July – 1st
August 2012, Cairns, Queensland, Australia
Paul Selleck, Frank Wong, Mia Kim, Nining Hartaningsih, Elly Sawitri, Pudjiatmoko,
Muhammad Azhar, Gwenaelle Dauphin, James McGrane, John Allen and Peter Daniels (2012)
Monitoring antigenic variation of H5N1 avian influenza viruses in Indonesia towards improved
vaccine selection. Emerging Infectious Diseases Symposium, October 22-23, 2012, Geelong,
Australia
Wang, J. (2012). Molecular diagnosis of emergency animal diseases at the Australian Animal
Health Laboratory. Paper presented at the Moleuclar Diagnostics Workshop 2012 - National
Centre for Infectious Disease and Biosecurity (NCBID), New Zealand, Wellington, New Zealand.
Wong, F. (2012). Science Presentation 4 - H5N1. Paper presented at the Emergency Animal
Disease Workshop, CSIRO Australian Animal Health Laboratory, Geelong, Vic.
Wong, F., Barr, I., Smith, D., Williams, D., Davies, K., Stevens, V., Daniels, P. (2012).
Identification of novel influenza A viruses in Australian swine. Paper presented at the AAVLD,
AgriBio Centre, DPIV, Bundoora, Vic.
Wong, F., Kim, M., Selleck, P., Stevens, V., Davies, K., Allen, J., Daniels, P. (2012). Update on
H5N1 activity and diversification in Indonesian poultry. Paper presented at the 8th International
Symposium on Avian Influenza, Royal Halloway, University of London, UK.
Wong, F., Selleck, P., Bruce, K., Allen, J., Claes, F., Kim, M., & Daniels, P. (2012).
International avian influenza activities at AAHL: contributions to one health from regional
veterinary surveillance programs. Paper presented at the Asia-Pacific Congress for Medical
Virology, Adelaide.
Annual reports of OIE Reference Laboratories, 2012
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