CURRICULUM VITAE
EUMORPHIA REMBOUTSIKA, Ph.D.
SET ROUTES UNIVERSITY AMBASSADOR(EMBO/CERN/EMBL)
PRINCIPAL INVESTIGATOR
STEM CELL BIOLOGY LABORATORΥ
BSRC ALEXANDER FLEMING
FLEMING 34, VARI 16672, GREECE
(ORGANIC POSITION;PHYSICAL PENDING)
Tel. 00 30 210 9656311. Fax. 00 30 210 9654934.
e-mail: remboutsika@gmail.com
AFFILIATED SCIENTIST University of Athens Medical
School, Department of Pediatrics, 75 Mikras Asias Str., 115
27, Athens, Greece. Tel. 00 30 210 77 94 023 Fax. 00 30
210 77 59 167. e-mail: remboutsika@med.uoa.gr
ADJUNCT FACULTY The Lieber Institute for Brain Development, Johns Hopkins Medical
School Campus, 855 North Wolfe Street, Suite 323
Baltimore, MD 21205, USA
SCIENTIFIC ADVISORY BOARD International Tissue Engineering Research Association
(ITERA-Life Sciences)(www.itera-ls.org/itera_committees.html)
DATE OF BIRTΗ
CITIZENSHIP
EXPERTISΕ
12 January 1966
PLACE OF BIRTH
GREEK
Athens, GREECE
LANGUAGES Greek,
English, French (basic)
Stem Cell Biology & Epigenetics
EDUCATION
Sept. 1984 - June 1988
Aug. 1988 - July 1994
B.Sc. in Chemistry, University of Patras, Patras, GREECE.
Ph.D. in Biochemistry and Molecular Biology Program,
Purdue University, West Lafayette, Indiana, U.S.A.
NATO Advanced Study Institute- FEBS Advanced Course
International Summer School on Developmental Biology:
"Organisation of the Early Vertebrate Embryo",
September 16-26, 1994, Spetsai, GREECE
Post-Doctoral Research Associate with Professor Pierre
Chambon, Member of the Academy of Sciences in USA
and France, Founder of the IGBMC and ICS, Strasbourg,
FRANCE.
Post-Doctoral Research Associate with Dr. Robin LovellBadge, Head of the Stem Cell Biology and Division of
Developmental Genetics, Fellow of the Royal Society of
Sciences (FRS) and Medicine (FRM) and Official
Spokesman of the Medical Research Council on Stem Cell
Research in the UK, MRC- NIMR, London, UK
Sept. 1994
Oct. 1994 - Oct. 1998
Jan. 1999 - Sept. 2003
SCIENTIFIC INTERESTS
TISSUE-SPECIFIC
POTENCY.
STEM
CELL
IDENTITY
AND
Understanding the intrinsic, extrinsic
and epigenetic mechanisms that establish and
maintain the tissue-specific identity of stem cells
when isolated or generated by differentiation
and/or by reprogramming.
1
RESEARCH EXPERIENCE
Aug. 1988 - Nov. 1990
Graduate Research Assistant with Dr. Jack E. Dixon.
Purdue University, West Lafayette, Indiana, USA.
Cloning and characterization of Protein Tyrosine
Phosphatases.
Dec. 1990 - July 1994
Graduate Research Assistant with Dr. Gunter B. Kohlhaw.
Purdue University, West Lafayette, Indiana, USA.
Transcriptional Regulator Leu3p of Yeast: Modular
architecture and function
Oct. 1994 - Oct. 1998
Post - Doctoral Research Associate with Professor Pierre
Chambon, IGBMC, Strasbourg, France.
Role of chromatin in the expression of retinoic acid
regulated genes
Jan. 1999 – Aug. 2003
Post - Doctoral Research Associate with Dr. Robin LovellBadge, NIMR, Mill Hill, London, UK.
Role of Sox2 in neural cell fate determination
Sept. 2003 - present
Principal Investigator, Institute of Molecular Biology and
Genetics, BSRC « Alexander Fleming », Athens, Greece.
Stem Cell Biology
Sept 2011 – present
Affiliated Scientist, University of Athens Medical School,
Department of Pediatrics, Athens, Greece. Stem Cell
Biology
July 2013- present
Adjunct Scientist, The Lieber Institute for Brain
Development, Johns Hopkins Medical Campus, Baltimore,
USA. Stem Cell Biology
TEACHING EXPERIENCE
Jan. - May 1990
Graduate Teaching Assistant, Biochemistry 322,
Purdue University, USA, Laboratory course.
Aug. - Dec. 1992
Graduate Teaching Assistant, Biochemistry 209,
Purdue University, USA, Laboratory course.
Jan. - May 1993
Graduate Teaching Assistant, Biochemistry 207,
Purdue University, USA, Lecture course.
Nov. 2004
Lecture on "Stem Cells: Biology and Applications",
Dept of Pharmacy, University of Patras, Greece.
Jan. 2005 - present
Annual Lecture on "Stem Cells: Biology and Applications",
Graduate Program in "Molecular Medicine", University of
Athens Medical School, Greece.
Apr. 2004- Apr. 2006
Lectures and Practical course on “Neural stem cells” in
“Applications of photon microscopy in biomedical research
and diagnosis”, Hellenic Pasteur Institute, Athens and
University of Crete Medical School, Heraklion, Greece.
Apr. 2005
Lecture on "Applications of Molecular Biology to Stem Cell
Research", Graduate Program in "Molecular Biology",
University of Athens Dentistry School, Greece.
Mar. 2005 - present
Biannual Lecture on "Neural Induction, Neural Fate
Stabilization and Neural Stem cells", Graduate Program in
2
"Neurosciences", University of Crete Medical School,
Heraklion, Crete, Greece.
Sept. 2007-present
Annual International Course on Laboratory Animal
science, Lecture on “Cloning and Stem Cells”, (FELASA),
BSRC “Alexander Fleming”, Vari, Athens, Greece.
Sept. 2010
Lectures on “Molecular and Cellular aspects of stem cells
& Somatic Cell cloning and Reprogramming”, Summer
School on Regenerative Medicine, Sept 6-12, 2010,
BRFAA, Athens, Greece.
HONORS AND AWARDS
1978 - 1983
Honour award - Ministry of Education (GREECE)
1980 - 1983
Excellency award -Ministry of Education (GREECE)
1982, 1983
Excellency award & medal – Municipality of Athens
(GREECE)
1984, 1987
University scholarship - State Scholarship Foundation
(GREECE)
June 1993
Graduate Student Travel award - Genetics Society of
America (USA)
1991, 1992
President, Hellenic Student Association, Purdue University
(USA)
1993 - 1994
Research Fellowship- Purdue Research Foundation (USA)
Oct. 1994 - June 1996
Marie Curie Fellowship - Human Capital and Mobility
Grant (EU)
July 1996 - June 1997
FRM Research Fellowship - Fondation pour la Recherche
Médicale (FRANCE)
July 1997 - Nov. 1997
Bourse GARCIN - Research Fellowship, Centre National
de la Recherche Scientifique (CNRS) (FRANCE)
Dec. 1997 - Oct. 1998
Research Fellowship – ADEREGEM (FRANCE)
Oct. 1998
Career Development Award (EPET II)- Department of
Biology, University of Crete, Heraklion, Crete (GREECE)
(awarded, not accepted)
Jan. 1999 – Mar. 2001
TMR Funded Post-Doctoral Research Associate (EU)
Apr. 2001 – Sept. 2003
MRC Funded Post-Doctoral Research Associate (UK)
Aug. 2003- Jul.2005
Career Development award (ENTER) – Institute of
Molecular Biology and Genetics (IMBG), Biomedical
Sciences Research Centre “Alexander Fleming” (BSRCFL), Athens (GREECE)
Sept. 2003- present
Principal Investigator, Stem Cell Biology Laboratory,
IMBG, BSRC-FL, Athens (GREECE)
2007-present
SET-Routes University Ambassador-Women in Science
(EMBO/CERN/EMBL)
3
2010-present
Scientific Advisory Board, International Tissue Engineering
Research Association (ITERA-Life Sciences)
July 2013- present
Adjunct Scientist, The Lieber Institute for Brain
Development, Johns Hopkins Medical School, Baltimore,
USA
INSTRUCTOR
Oct. 2004-present
Graduate Program in Neurosciences, University of Crete
Medical School, Heraklion, Crete (GREECE)
Apr. 2005-present
Graduate Program in Molecular Medicine, University of
Athens Medical School, Athens, (GREECE)
April 2004-April 2006
Greek Network for Light Microscopy (GREECE)
MEMBERSHIPS
Feb. 2005-present
Hellenic Society for Neurosciences (GREECE)
Feb. 2005-present
International Society for Stem Cell Research
Dec. 2005-present
Hellenic Society of Biochemistry and Molecular Biology
(GREECE)
SCIENTIFIC ADVISORY BOARD
2010-present
Scientific Advisory Board- International Tissue Engineering
Research Association (ITERA-Life Sciences)
2013-present
Scientific Advisory Board- The Lieber Institute for Brain
Development - Johns Hopkins Stem Cell Programme
WOMEN IN SCIENCE
2004-present
Women Researchers Network “PERIKTIONI” (Greece)
May 2007-present
SET-Routes University Ambassador (EMBO/CERN/EMBL)
May 2008-present
Women Academics Association (GREECE)
SUPERVISOR
1.
2.
3.
4.
5.
6.
VISITING SCIENTIST
FULBRIGHT STUDENT
PHD THESES
MASTER’S THESES
UNDERGRADUATE DIPLOMA THESES
POSTDOCTORAL FELLOWS
(COMPLETED: 1)
(COMPLETED: 1)
(COMPLETED: 4)
(COMPLETED: 3)
(COMPLETED: 5)
(IN PROGRESS:2)
POST-DOCTORAL FELLOWS
1. Nikolaos Mandalos, PhD. (2012- ) A role for Sox2 in the EMT during mouse
development
2. Theodoros Karnavas, PhD. (2013- ) A role for Sox2 in MET during direct lineage
reprogramming.
VISITING SCIENTISTS
4
Ioannis Karampelas, M.D. (Neurosurgery, USA) (July 2010- Dec. 2010 & July
2011- Dec. 2011). Targeting neural stem cells. External Supervisor: Prof. Robert
Miller, Vice Dean Medical School (Neurosciences), Case Western Reserve Medical
School.
FULBRIGHT STUDENT (USA)
Jessica Lea Harper, BSc. Sept 2008-2009, University of Illinois, Chicago,USA.
Current position: Permanent position at Regeneron Pharmaceuticals, Ltd., PhD
candidate, Columbia University, USA.
PHD THESES-COMPLETED
3. Theodoros Karnavas, PhD. (2005-2010) Epigenetic control in the establishment
and maintenance of a neural stem cell identity. University of Ioannina Medical
School, Ioannina, GREECE.
Academic Supervisor: Prof. Spyros Georgatos.
Current position: Joint Post-Doctoral position in Dr. E Remboutsika in a
collaboration with Marco Bianci’s lab, DIBIT-San Rafaelle, Milan, Italy.
4. Maria Poulou, MSc. (2006-2010) Mechanisms of a tissue-specific stem cell
regeneration. National and Kapodistrian University of Athens Medical School,
Athens, GREECE. Academic Supervisor: Prof. George Chrousos.
Past and Current position: Post-doctoral Researcher in Prof. Frank
Pajonk’s group, UCLA, Los Angeles, USA. Post-doctoral Researcher in
Dr. Michalis Alexis’ lab in National Research Foundation, Athens, Greece.
5. Maximilianos Elkouris, MSc. (2006-2010) Regulatory networks involved in
symmetric versus asymmetric cell division of cortical neural stem cells. Graduate
Program in Neurosciences, University of Crete Medical School, Crete, Greece.
Academic Supervisor: Prof. George Amoiridis.
Current position: Post-Doctoral Researcher in Dr. Iannis Talianidis’ group,
BSRC “Al. Fleming”.
6. Nikolaos Mandalos, MSc. (2006-2012) Generation of a conditional by inversion
Sox2 allele. Graduate Program in Neurosciences, University of Crete Medical
School, Crete, Greece.
Academic Supervisor: Prof. Kiki Thermos.
Current position: Post-Doctoral Researcher in Dr. Εumorphia
Remboutsika, BSRC “Al. Fleming”.
M ASTER’S THESES
7. Nikolaos Balaskas, BSc (2004-2006). A Role for Sox1 in neural stem cell state
and fate. Graduate Program in Neurosciences, University of Crete Medical School,
Crete, GREECE.
Current position: PhD (2010) (MRC Studentship) in Dr. James Briscoe’s group,
NIMR, London, UK. Post-Doctoral Researcher in Prof. Thomas Jessell’s group,
Columbia University Medical School, New York, USA.
8. Maria Poulou, BSc (2005-2007). Novel inducible gene expression system Leu3-αIPM in mice. Graduate Program in ”Molecular Medicine”, National and Kapodistrian
University of Athens Medical School, Athens, GREECE. Stem Cell Biology
Laboratory, BSRC “Al. Fleming”.
Current position: Post-doctoral Researcher in Prof. Frank Pajonk’s group, UCLA,
Los Angeles, USA.
9. Marannia Saridaki, BSc (2007-2009). Generation of a Sox2-eGFP allele
expressed in radial glia cells in the embryonic mouse brain. Graduate Program in
Neurosciences, University of Crete Medical School, Crete, GREECE.
5
Current position: PhD candidate (2011) in Dr. E. Andreakos group at BRFAA,
Athens, Greece.
UNDERGRADUATE DIPLOMA THESES
10. Maria Poulou (2004). Neural stem cell plasticity. Department of Molecular Biology
and Genetics, Democritian University of Thrace, Alexandroupolis, GREECE.
11. Athina Rodou (2004). Department of Molecular Biology and Genetics, Democritian
University of Thrace, Alexandroupolis, GREECE.
Current position: PhD candidate in Dr. Stathakopoulos’ group, UCLA, Pomona,
California, USA
12. Manos Metzakopian (2006). Sox2 Partners during Gastrulation. Department of
Biochemistry, University of Thessaly, Larissa, GREECE.
Current position: PhD (2010) (MRC Studentship) and Post-Doctoral Researcher in
Dr. Siew-Lan Ang’s group, NIMR, London, UK
13. Eleanna Stamatakou (2006). Epigenetic Landscape of the Sox2 gene during
Development. Department of Biochemistry, University of Thessaly, Larissa,
GREECE.
Current position: PhD candidate, in Dr. Patricia Selina’s group, University College
London (UCL), London, UK
14. Melania Kalaitzidou (2007). The Role of Retinoic Acid in the establishment of
Neural Progenitors. Department of Molecular Biology and Genetics, Democritian
University of Thrace, Alexandroupolis, GREECE.
Current position: PhD candidate (MRC Studentship) in Dr. Vassilis Pachnis’ group,
NIMR, London, UK
6
FUNDING
1.
(€ 632,450)
2003-2005
TOTAL NUMBER OF APPLICATIONS: 39
FUNDED: 11
SUCCESS RATE: 28%
ENTER- Career Development Award (01EP67) (€ 73,230)
What guides the transition from an ESC to an NSC?
Co-ordinator, Sponsor: Dr. Dimitris Thanos
Funding Organization: GSRT (GREECE)
2. 2004-2007
EPAN- National Network of Excellence (YB26) (€ 36,000)
NSC therapies for neurodegenerative diseases: determination
of a molecular signature for neuronal fate
Partner, Co-ordinator: Dr. Rebecca Matsas (HPI, GREECE)
Funding Organization: GSRT (GREECE)
3. 2004-2006
DESMI- RESEARCH GRANT (04/14)
(€ 38,250)
Evaluation of Sox1, Sox2 and ABCG2 as markers of neural
progenitor cells
Partner, Co-ordinator: Dr. Stavros Malas (CING, CYPRUS)
Funding Organization: RPF (CYPRUS)
4. 2005-2008
BILATERAL COOPERATION GRANT (NON-EU82)
(€ 72,000)
Engineering pure populations of primary NSC to express
cancer cell-specific killer cytokine TRAIL to be used as delivery
vehicles in cancer therapy
Co-ordinator, Partner: Dr. Aris Economides (Regeneron
Pharmaceuticals, Inc, USA) (Greece-USA partnership)
Funding Organization: GSRT (GREECE)
5. 2005-2008
PENEK- PhD Studentship Grant (05/04)
(€ 4,080)
Study of Sox1 during neurogenesis in the embryonic forebrain
External Supervisor, Co-ordinator: Dr. Stavros Malas (CING,
CYPRUS)
Funding Organization: RPF (CYPRUS)
6. 2005-2008
PENED2003- PhD Studentship Grant (03ΕΔ394)
(€ 50,736)
Generation and analysis of transgenic mouse models for
genetic diseases, neurodegeneration and regeneration of the
CNS
Partner,
Co-ordinator:
Dr.
Anthony
Gavalas
(FBRAA,GREECE)
Funding Organization: GSRT (GREECE)
7. 2005-2008
PENED2003- PhD Studentship Grant (03ΕΔ592)
(€ 51,195)
New biological study models for transcriptional regulation and
epigenetic control
Partner, Co-ordinator: Dr. Theodora Agalioti (IMBGFL,GREECE)
Funding Organization: GSRT (GREECE)
8. 2005-2008
IMBG-FL EXCELLENCE AWARD
(€ 60,000)
IMBG-FL Research Plan
Investigator, Director: Dr. Dimitris Thanos (IMBG-FL, GREECE)
Funding Organization: GSRT (GREECE)
CURRENT FUNDING
9.
2011-2014
“COOPERATION” –“ΣΥΝΕΡΓΑΣΙΑ” 09ΣΥΝ12-966 (€ 182,300)
Development of novel cell strategies to target myocardial
regeneration
7
Partner, Co-ordinator: Dr.Yiasemi Capetanaki (BRFAA, GREECE)
Funding Organization: GSRT (GREECE)
10.
11.
2012-2015
THALIS 945 – Academic Grants
(€ 40,000)
Synthesis and Characterization of new NANOstructrured
GEOpolymerS and CAlcium PHOsphate based Biocements and
Implants.
Partner, Co-ordinator: Prof. Stefanos Zaoutsos (TEI Larissas,
GREECE).
Funding Organization: Ministry of Education (GREECE)
2012-2015
THALIS 248 – Academic Grants
(€ 42,500)
Role and Mechanisms of asymmetric cell division in stem cell
differentiation.
Partner, Co-ordinator: Prof. Theodoros Fotsis (UoI, Medical
School GREECE)
Funding Organization: Ministry of Education (GREECE).
INDUSTRIAL RESEARCH COLLABORATION AGREEMENT
2004-today
RESEARCH COLLABORATION AGREEMENT
REGENERON PHARMACEUTICALS INC
Targeting of primary neural stem cells
COLLABORATOR : DR. ARIS ECONOMIDES, SENIOR DIRECTOR
GENOME ENGINEERING TECHNOLOGY DEVELOPMENT
COLLABORATORS
ONGOING PRIVATE SECTOR- NATIONAL AND INTERNATIONAL COLLABORATIONS
1. Screening for synthetic compounds in stem cell self-renewal and differentiation
Bionature, Ltd.(Nicosia, CYPRUS) and University of Crete Medical School
2. Homologous recombination in tissue-specific stem cells
Regeneron Pharmaceuticals, USA.
CURRENT PUBLIC SECTOR- NATIONAL COLLABORATIONS
1.
A role for natural and synthetic neurosteroids in self-renewal and differentiation of
neural stem cells
In collaboration with Prof. Achilleas Gravanis, University of Crete Medical School,
Heraklion, Crete, Greece.
2.
A role for natural and synthetic compounds in self-renewal and differentiation of
embryonic and tissue-specific stem cells
In collaboration with Prof. Leandros Skaltsounis, Pharmacy School, National and
Kapodistrian University of Athens, Athens, Greece.
3.
iMS function in mouse models of ischemic hearts
In collaboration with cardiologists Prof. Kostas Pantos, University of Athens
Medical School (Greece) and Dr. Yasemi Capetanaki (BRFAA, Athens, Greece)
8
CURRENT PUBLIC SECTOR- INTERNATIONAL COLLABORATIONS
1.
Retinoids and Sox genes
In collaboration with Prof. Pierre Chambon, IGBMC and ICS, Strasbourg,
FRANCE, Associate Prof. P. Dollé, IGBMC (France) and Dr. Robin LovellBadge, Head of the Division of Stem Cell Biology and Developmental
Genetics, MRC- NIMR, London, UK
2.
SoxB1 genes in cell cycle and neurogenesis
In collaboration with Dr. Stavros Malas, Developmental and Functional
Genetics,Cyprus Institute of Neurology and Genetics, Nicosia, CYPRUS.
REVIEWER
PEER-REVIEW JOURNALS
Cell, Developmental Biology, Nature Genetics,
PNAS, Journal of Molecular and Cellular Medicine,
Material Science and Engineering B,
European Cells and Materials Journal,
Tissue Engineering, Stem Cells
INTERNATIONAL GRANTS
1. Fundacao para Ciencia e a Technologia (FCT)
Ministry of Science, Technology and Higher
Education, PORTUGAL
2. Netherlands Organisation for Scientific
Research (NOW), NETHERLANDS
3. Alzheimer’s Society, UK
4. French National Research Agency (ANR)
EDITOR IN PEER REVIEW JOURNALS
2010 Review Editor at Frontiers in Physiology (Craniofacial Biology)
2013 Guest Review Editor at Frontiers in Physiology (Craniofacial Biology)
Currently Frontiers Research Topic Organizer on Stem cell Programming and
Reprogramming
European Cells and Materials Journal (eCM) (nominated, decision pending)
ORGANIZER & CHAIR
1.
2005 Workshop on Stem Cells, 39th Annual Scientific Meeting of the European
Society for Clinical Investigation, Athens, Greece, 6-9 April 2005. (ORGANIZERS: A.
Gavalas and E. Remboutsika).
2.
2005 Workshop on Stem Cell Biology "Neural Stem Cells", BSRC “ Alexander
Fleming”, Athens, Greece, 16 May 2005. (ORGANIZER).
3.
2008 SET-ROUTES conference on “Opening gateways to science”, National and
Kapodistrian University of Athens, November 11, 2008, Athens, Greece
(ORGANIZERS: Z. Lygerou, H. Boleti and E. Remboutsika).
4.
2008 International Greek Biotechnology Forum, Session Chair on “Stem Cells in
Clinical Applications”, 2nd February 2008, Athens, Greece (CHAIR).
9
5.
2009 60th Panhellenic Conference of the Hellenic Society of Biochemistry and
Molecular Biology, Session Chair on “Stem cells, Differentiation and Development”,
November 20-22, 2009, Zappeion, Athens (CHAIR).
6.
2011 2nd Stem Cells for Drug Developers, Workshop Leader “Exploiting Epigenetic
Mechanisms that control stem cell fate”. December 6 - 8, 2011, Berlin, Germany
(WORKSHOP LEADER).
CONFERENCE PARTICIPATION
POSTER PRESENTATIONS
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1991 75th Annual Meeting of the Federation of American Societies for Experimental
Biology (FASEB) Apr. 21-25, 1991, Atlanta, Georgia, U.S.A. Pot, D. A., T. A. Woodford,
E. Remboutsika, R. S. Haun and J. E. Dixon. Cloning, Bacterial Expression,
Purification and Characterization of the Cytoplasmic Domain of Rat LAR, a Receptorlike Protein Tyrosine Phosphatase. FASEB J. 5 (4): A833 [Part 1, March 11, 1991]
(Abstract #:2691).
1993 Keystone symposia on Molecular and Cellular Biology. Jan. 17-24, 1993,
Keystone, Colorado, U.S.A. Transcription: Factors, Regulation and Differentiation.
Sze, J., E. Remboutsika and G. B. Kohlhaw. The Leu3 protein: A metabolite dependent transcriptional activator which is also a repressor. Journal of Cellular
Biochemistry, Supplement 17A (Abstract #: B420).
1993 Yeast Genetics and Molecular Biology Meeting. Jun. 8-13, 1993, University of
Wisconsin-Madison, Madison, Wisconsin, U.S.A. Remboutsika, E., J. Sze and G. B.
Kohlhaw. Dissection of the activator and repressor functions of Leu3p of
Saccharomyces cerevisiae. Proceedings of Yeast Genetics and Molecular Biology
Meeting (Abstract #: 193B).
1994 Keystone symposia on Molecular and Cellular Biology. Feb. 13-20, 1994,
Keystone, Colorado, U.S.A. Basic Aspects of Transcription. Remboutsika, E. and G.
B. Kohlhaw. Structure - Function analysis of the transcriptional activation domain of
the Leu3 protein. Journal of Cellular Biochemistry, Supplement 18C (Abstract #:
L340).
1997 EMBO WORKSHOP. Chromatin and Epigenetic Regulation, Oct. 4-7, 1997,
EMBL Heidelberg, GERMANY. Remboutsika, E., M. Schmutz, Y. Lutz, A. Gansmuller,
J.-L. Vonesch, R. Losson and P. Chambon. The role of chromatin in the expression of
retinoic acid regulated genes. (Abstract #:102)
1998 80th Annual Meeting of the Endocrine Society, June 24-27, 1998, New Orleans,
Louisiana, U.S.A. Losson, R., N. Huang, A. L. Nielsen, E. Remboutsika, F. Cammas,
D. Metzger and P. Chambon. Chromatin-specific mediators for nuclear receptors.
1998 Third EMBL Meeting on Transcription, August 22-26, 1998, EMBL Heidelberg,
GERMANY. P. Chambon, R. Losson, F. J. Dilworth, C. Fromental-Ramain, N. Huang
and E. Remboutsika. Coregulators, chromatin and control of transcription by nuclear
receptors.
2000 Second Forum for European Neuroscience (FENS), June 24-28th, 2000,
Brighton, U.K. Remboutsika, E., K. Niederreither, M. Zile, P. Chambon and R. LovellBadge. Retinoids control the function of Sox2 in the developing neural tube. EUR J
NEUROSCI 12: 328-328 Suppl. S 2000
2001 Cold Spring Harbor Laboratory Meeting. “Stem and progenitor cells: Biology and
Applications”. March 22-25, 2001. Cold Spring Harbor, New York, USA. Remboutsika,
E., A. A. Avilion, S. Sockanathan,, M. Cohen-Tannoudji and R. Lovell-Badge. Nuclear
SOX2 protein marks the multipotent lineage in mouse embryos.
2001 MRC Center for Developmental Neurobiology - Inaugural Meeting. “The
Developing and Regenerating Brain”. September 5-8, 2001. King’s College, London,
UK. Remboutsika, E., P. Trainor, K. Niederreither, P. Chambon and R.Lovell-Badge.
Sox2 interprets the retinoid signal along the neural tube.
2002 Fifth EMBL Meeting on Transcription, August 24-28, 2002, EMBL Heidelberg,
GERMANY. Remboutsika, E., K. Yamamoto, M. Harbers and M. Schmutz. The
bromodomain mediates TIF1α-nucleosome interactions.
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2002 Route 28 Summits in Neurobiology. “Stem cell based therapeutic approaches for
Parkinson’s disease”. August 22-28, 2002. Frauenchiemsee, Germany. Antoniadis,
C.L., E. Remboutsika, P. Trainor and R. Lovell-Badge. Comparative studies of pure
populations of neural stem cells.
2002 MRC meeting on “Stem Cells: Prospects for Research and Therapy”. September
11, 2002. London. United Kingdom. Remboutsika, E., C. L. Antoniadis, P. Trainor and
R. Lovell-Badge. Comparative studies of pure populations of neural stem cells.
2003 6th Meeting of the UK Glial Cell Club on “ Glia: Role in neural development and
repair”. February 26, 2003. London, UK. Remboutsika, E., P. Trainor, C. L.
Andoniadou and R. Lovell-Badge. Neural stem cell identity and fate depend on Sox2.
2005 1st ESH-EMBT-EUROCORD Euroconference on Stem Cell Research, April 15-17,
2005, Cascais, PORTUGAL. Balaskas, N., M. Poulou, A. Kotsoni, V. Episkopou and E.
Remboutsika. A Role for Sox1 in Neural Stem Cell State and Fate (Abstract P8).
2005 1st ESH-EMBT-EUROCORD Euroconference on Stem Cell Research, April 15-17,
2005, Cascais, PORTUGAL. Poulou, M., A. Kotsoni, D. Stemple, D. Bell, A. Gavalas and
E. Remboutsika. Re-birth of a Neural Stem Cell (Abstract P32).
2005 Cortical Development "Neural Stem Cells to Neural Circuits", May 12-15, 2005,
Santorini, GREECE. Remboutsika, E., Trainor, P.A., Andoniadou, C., and Lovell-Badge,
R. SOX2 and the intrinsic and extrinsic control of neural stem cell identity (Abstract P76).
2005 19th Annual Meeting of the Hellenic Society for Neuroscience. September 30October 2, 2005, Patras, GREECE. Balaskas, N., M. Poulou, A. Kotsoni, V. Episkopou
and E. Remboutsika. A Role for Sox1 in Neural Stem Cell State and Fate (Abstract P5).
2005 57th Meeting of the Hellenic Society of Biochemistry and Molecular Biology.
December 9-11, 2005, Athens, GREECE. Balaskas, N., M. Poulou, A. Kotsoni, V.
Episkopou and E. Remboutsika. A Role for Sox1 in Neural Stem Cell State and Fate
(Abstract P).
2005 57th Meeting of the Hellenic Society of Biochemistry and Molecular Biology.
December 9-11, 2005, Athens, GREECE. Poulou, M., A. Kotsoni, D. Stemple, D. Bell, A.
Gavalas and E. Remboutsika. Re-birth of a Neural Stem Cell (Abstract P32).
2006 58th Meeting of the Hellenic Society of Biochemistry and Molecular Biology.
September 14-16, 2006, Patras, GREECE. Balaskas, N., Poulou, M., A. Kotsoni,
Episkopou, V., and E. Remboutsika. Sox1 is required in the initial steps of neural stem
cell proliferation for the suppression of neurogenesis (Abstract P36).
2006 58th Meeting of the Hellenic Society of Biochemistry and Molecular Biology.
September 14-16, 2006, Patras, GREECE. Karnavas, T., A. Kotsoni, E. Stamatakou and
E. Remboutsika. Sox2 is an intrinsic regulator of primitive ectoderm formation from
embryonic stem cells (Abstract P104).
2008 MUGEN Conference Denis, M.C., Remboutsika, E. and Kollias, G. Molecular
mechanisms of NPC-mediated repair of autoimmune demyelination. Blood cells
molecules and diseases 40(2), 262-263 (Abstract 24) Mar-Apr. 2008, Athens, GREECE.
2008 Cortical Development "Stem cells, Neurogenesis, Migration, Circuit Formation,
Cortical Disorders", May 23-25, 2008, Chania, GREECE. Elkouris, M., Balaskas, N.,
Poulou, M., Episkopou, V., and Remboutsika, E. Opposing roles for SOX1 and SOX2 in
cortical neural stem/progenitor cells (Abstract P22).
2008 Cortical Development "Stem cells, Neurogenesis, Migration, Circuit Formation,
Cortical Disorders", May 23-25, 2008, Chania, GREECE. Seglia, A, Elkouris, M.,
Zouvelou, V., Remboutsika, E., and Graf, D. Multiple roles for BMP7 in the development
of the mouse cortex (Abstract P35)
2009 23rd Annual meeting of Hellenic society for Neuroscience, September 13-16, 2009,
Rhodes, GREECE. Elkouris, M., Balaskas, N., Poulou, M., Politis, P., Thomaidou, D.,
Episkopou, V., and Remboutsika, E. SOX1 suppresses asymmetric cell division in
proliferating neural stem/progenitor cells. (Abstract P1).
2009 Reprogramming Cell Fate: Basic Biology and Medical Perspectives, December 1113, 2009, Milan, ITALY. Poulou, M. and Remboutsika, E. Transgene-free inducible
reprogramming of fibroblasts into muscle stem/progenitor cells (iMS). (Abstract A29).
2010 Advanced Light Microscopy Symposium “Imaging Biomolecules in Time and
Space”, 24-26 September 2010, Conference Center of the University of Patras, University
Campus, Rio Patras, Greece. Elkouris, M., Balaskas, N., Poulou, M., Politis, P.,
Panayiotou, E., Malas, S., Thomaidou, D., and Remboutsika, E. SOX1 maintains the
undifferentiated state of cortical neural progenitor cells via suppression of Prox1-mediated
cell cycle exit and neurogenesis. (Abstract M1).
2010 Neuroscience Days of the Hellenic Society for Neuroscience, October 1-2, 2010,
11
30.
31.
32.
33.
34.
35.
36.
37.
Democritus Institute, Athens, Greece. Elkouris, M., Balaskas, N., Poulou, M., Politis, P.,
Panayiotou, E., Malas, S., Thomaidou, D., Episkopou, V., and Remboutsika, E. SOX1
maintains the undifferentiated state of cortical neural progenitor cells via suppression of
Prox1-mediated cell cycle exit and neurogenesis. (Abstract P1).
2010 24th International Mammalian Genome Conference (IMBG), October 17-21, 2010.
Aldemar Knossos Royal Village, Heraklion Crete, Greece. Mandalos, N., Saridaki, M.,
Harper, J.L., Kotsosni, A., Economides, A. N., and Remboutsika, E. Epiblast-ablation of
Sox2 expression leads to neurocristopathies in mouse embryos. (Abstract 142).
2011 Cortical Development "Neural stem cells to Neural Circuits", May 19-22, 2011,
Chania, GREECE. Elkouris, M., Balaskas, N., Poulou, M., Politis, P., Panayiotou, E.,
Malas, S., Thomaidou, D., Episkopou, V., and Remboutsika, E. SOX1 maintains the
undifferentiated state of cortical neural progenitor cells via suppression of Prox1-mediated
cell cycle exit and neurogenesis.
2011 Stem Cell Programming and Reprogramming, December 8-11, 2011, Lisbon,
PORTUGAL. Poulou, M., Karnavas, T. and Remboutsika, E. A “hit and run” approach to
accelerate lineage reprogramming. (Abstract 0130).
2011 Stem Cell Programming and Reprogramming, December 8-11, 2011, Lisbon,
PORTUGAL. Karnavas, T., Kotsoni, A., DeGrigorio, A., Stamou, P., Kontoyiannis, D.,
Rodriguez, T. A. and Remboutsika, E. A Sox2-instructed conversion of mouse
embryonic stem cells to primed epiblast bodies (Epispheres). (Abstract 0131).
2011 Stem Cell Programming and Reprogramming, December 8-11, 2011, Lisbon,
PORTUGAL. Segklia, A., Seutjens, E., Elkouris, M., Tsalavos, S., Mitsiadis, T.,
Huylebroeck, D., Remboutsika, E.,* and Graf, D.*. BMP7 regulates the survival,
proliferation and neurogenic properties of neural progenitor cells during corticogenesis in
the mouse. (*corresponding authors). (Abstract 0132).
2012 63rd Conference of the Hellenic Society of Biochemistry and Molecular Biology,
November 9-11, 2012, ITE, Heraklion, Crete, Greece. Mandalos, N., Poulou, M.,
Karnavas, T., Rhinn, M., Economides, A. N., Dolle, P., and Remboutsika E. A novel role
for Sox2 as a rheostat of epithelial to mesenchymal transition. (Abstract 190).
2012 Integer Conference, 14-16th November, 2012, Athens, Greece. Mandalos, N.,
Poulou, M., Karnavas, T., Rhinn, M., Economides, A. N., Dolle, P., and Remboutsika E.
A novel role for Sox2 as a rheostat of epithelial to mesenchymal transition.
2012 Integer Conference, 14-16th November, 2012, Athens, Greece. Karnavas, T.,
Kotsoni, A., DeGrigorio, A., Stamou, P., Kontoyiannis D.L., Rodriguez, T. A. and
Remboutsika E. A novel mode of pluripotency.
PLATFORM PRESENTATIONS
1.
2.
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4.
5.
6.
7.
1993 Yeast Genetics and Molecular Biology Meeting. June 8-13, University of
Wisconsin-Madison, Madison, Wisconsin, USA. Workshop on "Biochemical and
Genetic analysis of Transcription". Remboutsika, E. Transcriptional regulator Leu3 of
yeast: Dissection of activator and repressor functions.
1997 11th Balkan Biochemical Biophysical Days. May 15-17, Thessaloniki, GREECE.
Remboutsika, E., K. Niederreither, P. Dollé and P. Chambon. Role of chromatin in the
expression of retinoic acid regulated genes.
2000 Mammalian Development and Genetics workshop. Institute of Child Health.
November 29- December 1, 2000. London, UK. Remboutsika. E., P. Trainor, K.
Niederreither, M. Zile, P. Chambon and R. Lovell-Badge. Sox2 interprets the retinoid
signalling in the developing ventral neural tube.
2001 Cold Spring Harbor Laboratory Meeting. “Stem and progenitor cells: Biology and
Applications”. March 22-25, 2001. Cold Spring Harbor, New York, USA. Remboutsika.
E. and R. Lovell-Badge. Sox2: A marker for neural stem cells in the mouse.
2001 Mouse Molecular Genetics Meeting, August 22-26, 2001, EMBL Heidelberg,
GERMANY. Remboutsika, E., P. Trainor, K. Niederreither, P. Chambon and R. LovellBadge. Sox2 interprets the retinoid signal along the neural tube.
2003 Mouse Molecular Genetics Meeting, September 3-7, 2003, EMBL Heidelberg,
GERMANY. Remboutsika, E., P. Trainor and R. Lovell-Badge. Neural stem cell
identity and fate depend on Sox2 expression.
2008 60th Panhellenic Conference of the Hellenic Society of Biochemistry and
Molecular Biology, November 20-22, 2009, Zappeion, Athens. Karnavas, T., Kotsoni,
A. and Remboutsika, E. A Sox2-instructed conversion of embryonic stem cells to
12
primed epiblast cells.
2012 63rd Conference of the Hellenic Society of Biochemistry and Molecular Biology,
November 9-11, 2012, ITE, Heraklion, Crete, Greece. Karnavas, T., Kotsoni, A.,
DeGrigorio, A., Stamou, P., Kontoyiannis D.L., Rodriguez, T. A. and Remboutsika E.
A novel mode of pluripotency.
8.
INVITED LECTURES
CONFERENCES
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2003 18th Meeting of the Hellenic Society for Neuroscience. October 17-19, 2003,
Institut Pasteur Hellenique, Athens, GREECE. Remboutsika, E., P. Trainor and R.
Lovell-Badge. Embryonic cortical neural stem cells, marked by Sox2 expression,
can generate neural crest.
2003 55th Meeting of the Hellenic Society of Biochemistry and Molecular Biology.
Mouse Molecular Genetics Meeting, November 13-15, 2003, FBRAA, Athens,
GREECE. Remboutsika, E., P. Trainor and R. Lovell-Badge. Embryonic cortical
neural stem cells, marked by Sox2 expression, can generate neural crest.
2003 2nd Meeting on "Genetic Analysis". December 19-20, 2003, Diagnostic and
Therapeutic Center Hygeia (Harvard International), Athens, GREECE.
Remboutsika, E. Sox2: a neural stem cell identity factor.
2004 Meeting on "Recent advances in Pathophysiology and Treatment of
Parkinson Disease". September 10-11, 2004, Foundation for Biomedical Research
of the Academy of Athens. Remboutsika, E. Acquiring a neural stem cell identity.
2005 1st Symposium on "Research and Technology in Greece". January 27-28,
2005, Hellenic Research Foundation, Athens, Greece. Remboutsika, E. Stem Cell
Research in Greece: Socioeconomics.
2005 39th Annual Scientific Meeting of the European Society for Clinical
Investigation. Workshop 20 on "Stem Cells" (co-organizer). April 6-9, 2005, Athens,
Greece. Remboutsika, E. Neural Stem cell Commitment to a Radial Glia identity.
2005 Modern Methods of Light Microscopy and Applications in Biomedical
Research and Diagnosis. April 18-22, 2005, Hellenic Pasteur Institute, Athens,
Greece. Remboutsika, E. A confocal view of a stem cell.
2005 Workshop on " Stem Cell Biology - Neural Stem Cells" (organizer). May 16,
2005, Biomedical Sciences Research Centre " Alexander Fleming", Athens,
Greece. Remboutsika, E. Intrinsic control of neural stem cell identity.
2005 Modern Methods of Light Microscopy and Applications in Biomedical
Research and Diagnosis. November 1-5, 2005. University of Crete Medical School,
Heraklion Crete, Greece. Remboutsika, E. A confocal view of a stem cell.
2006 16th Panhellenic Training Seminar on Gynecological Endocrinology. Session
on “Biology and Technology of Stem Cells: Current position and perspectives”.
January 28-29, 2006. Athens, Greece. Remboutsika, E. Embryonic stem cells:
Molecular parameters of self-renewal and differentiation.
2006 5th Conference of the Greek-German Academy in Biomedical Research. New
trends in Biomedical Research. June 16-18, 2006. University of Athens Medical
School, Athens, Greece. Remboutsika, E. Sox2 maintains self-renewal and dorsal
radial glia identity and inhibits cortical neural stem cell commitment to a cranial
neural crest cell fate.
2006 58th Meeting of the Hellenic Society of Biochemistry and Molecular Biology.
September 14-16, 2006, Patras, GREECE. E. Remboutsika. Sox2 is an intrinsic
regulator of cortical neural stem cells.
2008 International Greek Biotechnology Forum, Session on “Stem Cells in Clinical
applications”, Zappeion 2-3rd February 2008, Athens, Greece. Remboutsika, E.
Birth and Life of a Neural Stem Cell.
2008 5th Panhellenic Conference on Pharmacology, Round Table on “Regenerative
Pharmacology”, May 23-25, 2008, Athens, Greece. Remboutsika, E. Neural Stem
Cells.
2008 33rd FEBS Congress & 11th IUBMB Conference, Science and Society on
“Biochemistry-Quality of Life- New Challenges”, June 28th-July 3rd, 2008, Athens.
Remboutsika, E. Stem Cells.
2011 5th ITERA-Life Sciences Conference, November 20th -22nd, 2011, Maastricht,
13
17.
18.
Netherlands. Remboutsika, E. Direct Transgene-free inducible reprogramming of
fibroblasts to muscle stem/progenitor cells.
2011 2nd Stem Cells for Drug Developers, December 6th-8th, 2011, Berlin,
Germany. Workshop Leader. Remboutsika, E. Exploiting Epigenetic Mechanisms
that control stem cell fate.
2012 1st Hellenic Stem Cell Network Meeting, January 26-27th, 2012, Athens,
Greece. Remboutsika, E. Stemness settles SoxB genes down to work?
RESEARCH ESTABLISHMENTS
1.
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Remboutsika E. (1993). Transcriptional Regulator Leu3p of Yeast: Modular
architecture and function, December 20, 1993. EMBL, Heidelberg, GERMANY.
Remboutsika E. (1993). Transcriptional Regulator Leu3p of Yeast: Modular
architecture and function, December 21, 1993. Laboratoire de Genetique
Moleculaire des Eukaryotes (LGME), Strasbourg, FRANCE.
Remboutsika E. (1997). The role of chromatin in the expression of retinoic acid
regulated genes. May 8, 1997 Institute of Molecular Biology and Biotechnology
(IMBB-FORTH), Herakleion, Crete, GREECE.
Remboutsika E. (1999). Epigenetic factors in mouse development. Club Dynamic
Fonctionnelle de la Chromatine. May 7, 1999. Institut Jacques Monod, Paris,
FRANCE.
Remboutsika E. (2000). Epigenetic factors in mouse development. October 4,
2000. Chester Beatty Laboratories, Institute of Cancer Research, London, UK.
Remboutsika E. (2001). The brain pulls its SOX up. December 11, 2001.
Biomedical Sciences Research Center “Alexander Fleming”, Institute of Molecular
Biology and Genetics, Athens, GREECE.
Remboutsika E. (2001). A role for Sox2 in the CNS. December 18, 2001.
University of Patras, Medical School, Patras, GREECE.
Remboutsika E. (2002). A role for Sox2 in neural cell fate determination. January
16, 2002. Wolfson Institute for Biomedical Research, University College of London
(UCL), London, UK.
Remboutsika E. (2004). Sox2: A neural stem cell identity factor. January 28, 2004.
Hellenic Pasteur Institute, Athens, GREECE.
Remboutsika E. (2005). Neural stem cells: an identity crisis? February 16, 2005.
Department of Biology, University of Athens, GREECE
Remboutsika E. (2005). Stem cells on the verge of a nervous breakdown. March
7, 2005. University of Crete Medical School, Heraklion, Crete, GREECE
Remboutsika E. (2005). Intrinsic Control of Neural Stem cell Identity. September
28, 2005. Institute of Biology and Physical Chemistry, Demokritos National Centre
for Scientific Research, Athens, GREECE
Remboutsika E. (2006). Pull your SOX up and walk. October 10, 2006. University
of Crete Medical School, Heraklion, Crete, GREECE.
Remboutsika E. (2008). Stem cells pull their SOX up. June 3, 2008. Institute of
Biology and Physical Chemistry, Demokritos National Centre for Scientific
Research, Athens, GREECE.
Remboutsika E. (2008). Stem cells pull their SOX up. Nov 17, 2008. Regeneron
Pharmaceuticals, Inc. Tarrytown, New York, USA.
Remboutsika E. (2009). “Hit and Run” reprogramming of somatic cells to tissuespecific stem cells. November 27, 2009. University of Patras Medical School,
Patras, GREECE.
Remboutsika E. (2011). “Stemness settles SoxB genes down to work?” January
27, 2011. Institute of Biology and Physical Chemistry, Demokritos National Centre
for Scientific Research, Athens, GREECE.
Remboutsika E. (2013). “SoxB settle stemness down to work” May 11, 2013.
SCIENCE AND SOCIETY LECTURES
1.
2.
2005 1st Symposium on "Research and Technology in Greece". January 27-28, 2005,
Hellenic Research Foundation, Athens, Greece. Remboutsika, E. Stem Cell
Research in Greece: Socioeconomics.
2008 33rd FEBS Congress & 11th IUBMB Conference, Science and Society on
14
3.
“Biochemistry-Quality of Life- New Challenges”, June 28th-July 3rd, 2008, Athens.
Remboutsika, E. Stem Cells.
2008 European Researchers Night 2008 event, Larissa, Greece. September 26 th,
2008. Remboutsika, E. Stem Cells.
WOMEN IN SCIENCE LECTURES
1.
2.
3.
2008 Hellenic Association of Women Scientists. July 3rd, 2008. Remboutsika, E.
University of Pireaus. Investigating disease pathways.
2008 MUGEN Network of Excellence conference. Nov. 11th, 2008. Remboutsika,
E. Athens. SET-ROUTES-Women in Science.
2008 SET-ROUTES conference “Opening gateways to science”, National and
Kapodistrian University of Athens, November 11, 2008, Athens, Greece
(ORGANIZERS: Z. Lygerou, H. Boleti and E. Remboutsika). An Intercontinental
Flight from Chemistry to Stem Cell Biology.
REFERENCES
1. Prof. Pierre Chambon, Institut de Génétique et de Biologie Moléculaire et Cellulaire,
Strasbourg, France. E-mail: chambon@igbmc.fr
2. Dr. Ronald McKay, Lieber Institute for Brain Development, John Hopkins University,
Baltimore, USA. E-mail: ronaldmckay@mac.com
3. Prof. George Chrousos, University of Athens Medical School, Athens, Greece. E-mail:
chrousog@exchange.nih.gov
4. Dr. Aris Economides, Regeneron Pharmaceuticals, Tarrytown, New York, USA. E-mail:
aris.economides@regeneron.com
5. Prof. Thimios Mitsiadis, Institute of Oral Biology, University of Zurich, Zurich,
Switzerland. E-mail: thimios.mitsiadis@zzmk.uzh.ch.
15
PUBLICATIONS IN PEER-REVIEW JOURNALS
1988-1994
1. Pot, D. A., T. A. Woodford, E. Remboutsika, R. S. Haun and J. E. Dixon. (1991).
Cloning, Bacterial Expression, Purification and Characterization of the Cytoplasmic
Domain of Rat LAR, a Receptor-like Protein Tyrosine Phosphatase. J. Biol. Chem.
266: 19688 – 19696.
2. Sze, J., E. Remboutsika, and G. B. Kohlhaw. (1993). Transcriptional Regulator Leu3
of Yeast: Separation of Activator and Repressor Functions. Mol. Cell. Biol. 13: 5702 –
5709.
3. Remboutsika, E. and G. B. Kohlhaw. (1994). Molecular Architecture of a Leu3p DNA Complex in Solution: A Biochemical Approach. Mol. Cell. Biol. 14: 5547 - 5557.
1994-2003
4. Dilworth, J. F, K. Fromenthal-Ramain, E. Remboutsika, A. Benecke and P. Chambon.
(1999). Ligand-dependent activation of transcription in vitro by RARα/RXRα
heterodimers that mimic transactivation by retinoids in vivo. Proc. Natl. Acad. Sci.
96: 1995 - 2000.
5. Remboutsika, E., Y. Lutz, A. Gansmuller, J. -L. Vonesch, R. Losson and P.
Chambon. (1999). The putative nuclear receptor mediator TIF1α is tightly associated
with euchromatin. J. Cell Sci. 112: 1671-1683.
6. Niederreither, K. *, E. Remboutsika*, A. Gansmuller, R. Losson, P. Dollé and P.
Chambon (1999). Tissue-specific expression of transcriptional intermediary factor
TIF1α during mouse development and in the reproductive organs. Mech. Dev. 88:
111-117 (*equal first authors).
7. Remboutsika E., X. Jacq and L. Tora (2001). Chromatin is permissive to TATAbinding protein (TBP)-mediated transcription initiation. J. Biol. Chem. 276: 12781 12784.
8. Nielsen, A. L., Oulad-Abdelghani, M., Ortiz, J. A., Remboutsika, E., Chambon, P and
R. Losson (2001). Heterochromatin formation in mammalian cells: interactions
between histones and HP1 proteins. Mol. Cell. 7: 729 - 739.
9. Remboutsika, E.*, K. Yamamoto, M. Harbers and M. Schmutz (2002). The
bromodomain mediates TIF1α-nucleosome interactions. J. Biol. Chem. 277: 50318 50325 (*corresponding author).
2003-2013
10. Charalambopoulos, G., Remboutsika, E., Margioris, A. and Gravanis, A. (2008).
Neurosteroids as endogenous modulators of Neurogenesis and Neuronal survival.
Trends in Endocrinology and Metabolism 19(8), 300-307.
11. Fotopoulou, S., Oikonomou, N., Grigrorieva, E., Nikitopoulou, I., Paparountas, T.,
Thanassopoulou, A., Zhao, Z., Xu, Y., Kontoyiannis, D., Remboutsika, E., and
Aidinis, V. (2010). ATX and LPA signaling are necessary for embryonic nervous
system development, Developmental Biology 339 (2): 451-464.
12. Poulou, M., Bell, D., Bozonelos, K., Alexiou, M., Gavalas, A., Lovell-Badge, R., and
Remboutsika, E. (2010). Development of a chromosomally integrated metaboliteinducible Leu3p-α-IPM “OFF-ON” switch. PLOS One 5(8): e12488.
13. Catón, J., Bostanci, .N., Remboutsika, E., De Bari, C., and Mitsiadis, T. A. (2010).
Future dentistry: cell therapy meets tooth repair and regeneration. Journal of Cellular
16
and Molecular Medicine, Dec 28, doi: 10.111/j.1582-4934.2010.01251.x [Epub
ahead of print], PMID: 21199329.
14. Elkouris, M., Balaskas, N., Poulou, M., Politis, P., Panayiotou, E., Malas, S.,
Thomaidou, D., and Remboutsika, E. (2011). Sox1 maintains the undifferentiated
state of cortical neural progenitor cells via suppression of Prox1-mediated cell cycle
exit and neurogenesis, STEM CELLS, Jan. 28, 29 (1): 89-98, doi: 10.1002/stem.554.
(Oct 22, 2010 [Epub ahead of print], PMID: 20973031).
15. Remboutsika, E*., Elkouris, M., Iulianella, A., Andoniadis, C. L., Poulou, M.,
Mitsiadis, T., Trainor, P. and R. Lovell-Badge (2011). Flexibility of neural stem cells,
Frontiers in Physiology 2: 16 (April 2011) [doi: 10.3389 / fphys.2011.00016]
(*corresponding author).
COMMENTARY: Raitano, S., Verfaillie, C.M. and Pertyk, A. (2013). Self-renewal of
neural stem cells: implications for future therapies. Frontiers in Physiology 4:49
(doi:10.3389 / fphys.2013.00049).
16. Sgantzis, N., Yiakouvaki, A., Remboutsika, E. and Kontoyiannis, D. (2011). HuR
regulates lung branching morphogenesis and mesenchymal FGF networks.
Developmental Biology 354(2): 267-279 [Epub ahead of print], PMID: 21515253).
17. Segklia, A., Seutjens, E., Elkouris, M., Tsalavos, S., Mitsiadis, T., Huylebroeck, D.,
Remboutsika, E.,* and Graf, D.* (2012). BMP7 regulates the survival, proliferation
and neurogenic properties of neural progenitor cells during corticogenesis in the
mouse, PLOS One 7(3): e34088.doi: 10.1371/journal.pone.0034088. (*equal
corresponding authors).
18. Mandalos, N., Saridaki, M., Kotsoni, A., Harper, J. L., Yang, P., Economides, A. and
Remboutsika, E. (2012). Application of a novel strategy of engineering conditional
alleles to a single exon gene, Sox2. PLOS One, 7(9) : e45768.
19. Koutmani, Y., Politis, P., Elkouris, M., Argogiannis, G., Kemrli, M., Patsouris, E.,
Remboutsika, E. and Karalis, K. P. (2013). A novel role for Corticotropin-Releasing
Hormone (CRH) in the regulation of neurogenesis, Molecular Psychiatry 18, 300307.
20. Karnavas, T*., Mandalos, N*., Malas, S. and Remboutsika, E. (2013). SoxB, cell
cycle and neurogenesis. Frontiers in Physiology (in press) (*equal authors).
In the process of imminent submission/preparation (abstracts available upon
request)
21. Mandalos, N., Poulou, M., Karnavas, T. and Remboutsika, E. (2013). From Stem
Cells to Organs (Invited Review). Frontiers in Physiology (imminent
submission).
22. Poulou, M., Mandalos, N., Karnavas, T. and Remboutsika, E. (2013). Inducible
gene expression in stem cell programming and reprogramming (Invited Review).
Frontiers in Physiology (imminent submission).
23. Karnavas, T., Kotsoni, A., DeGrigorio, A., Stamou, P., Kontoyiannis, D., Rodriguez, T.
A. and Remboutsika, E.( 2013). Epispheres: A novel mode of pluripotency, in
preparation for submission.
24. Mandalos, N., Poulou, M., Karnavas, T., Rhinn, M., Economides, A., Dollè, and
Remboutsika, E. (2013a). A novel role for Sox2 as a rheostat of epithelial to
mesenchymal transition during neural crest development, in preparation.
17
25. Mandalos, N., Poulou, M. Karnavas, T., Rhinn, M., Dolle, P. and Remboutsika, E.
(2013b). Sox2 accelerates direct lineage reprogramming, in preparation
26. Kalaitzidou, M., M. Poulou, P. Trainor, K. Niederreither, P. Chambon, P. Dolle, R.
Lovell-Badge and Remboutsika, E. (2013). Sox2 interprets the retinoid signal in the
developing spinal cord, in preparation.
PATENTS
2008
NEUROSTEROID COMPOUNDS, co-inventor,
Applicant: Bionature Ltd,
WO/2008/155534
2013 Patent Granted
2010
NEUROSTEROID COMPOUNDS, co-inventor,
Applicant: Bionature Ltd,
US2010/0234335 A1
2013 Imminent decision
2010
NEUROSTEROID COMPOUNDS, co-inventor,
Applicant: Bionature Ltd,
EP2158209
2013 Decision Pending
2013
“Hit and Run” direct reprogramming of somatic cells to
tissue-specific stem cells, in preparation
(funded by Synergasia 12-SYN-09-966)
PUBLICATIONS IN POPULAR SCIENCE JOURNALS
1.Remboutsika, E. (2007). To Stem or not to stem? MED Review Laboratory 3, pp 42-45.
2.Remboutsika, E. (2008). Stem cell biology at a glance. BIO 23, pp 42-43.
18
CURRENT AND FUTURE RESEARCH
REMBOUTSIKA LABORATORY
STEM CELL BIOLOGY
TISSUE-SPECIFIC STEM CELL IDENTITY AND POTENCY (STEMNESS). Understanding how stem cells
arise and guard their identity is crucial for the development of novel therapies for tissue/organ
regeneration and tumour suppression.
Research in our Stem Cell Biology Laboratory aims to understand how cells acquire and
maintain their tissue-specific stem cell identity and potency.
In the past seven years, we have studied the properties of tissue-specific stem cells derived
from the:

Differentiation of Sox2-selected embryonic stem cells into primed epiblast (Epispheres),
for the study of the genetic and epigenetic regulators of stem cell potency

Isolation of lineage-selected stem cell populations from the embryonic central nervous
system for the study of symmetric cell division in neural stem and progenitor cells

Direct Reprogramming of somatic cells to tissue-specific stem cells via novel nonintegrated single factor dependent “hit and run“ approach developed in our laboratory
(fibroblasts to induced muscle stem cells (iMS) and astrocytes to induced neural stem
cells (iNS)).
Using maintenance-of function approaches in primary and embryonic stem cells, gain-offunction approaches during lineage reprogramming and conditional loss-of-function
approaches, we have obtained evidence for:

a role for SoxB1 genes and signaling molecules in the Regional identity and self-renewal
properties of embryonic neural stem and progenitor cells (Fotopoulou et al., 2010;Elkouris
et al., 2011; Remboutsika et al., 2011; Segklia et al., 2012; Koutmani et al., 2013;
Karnavas et al., 2013, submitted; Kalaitzidou et al., 2013, in prep).

the generation of primed epiblast cells from ESC cells as Epispheres, possessing a novel
mode of pluripotency with no tumour formation properties (Karnavas et al., 2013, in
prep).

rapid and efficient direct reprogramming of mouse somatic cells to induced tissue-specific
stem cells (iTS) (iMS, muscle; iNS, nervous system) (Poulou et al., 2010; Mandalos et al.,
2013b, in preparation).

a key role for Sox2 in epithelial to mesenchymal transition (EMT) and mesenchymal to
epithelial transition (MET) (Mandalos et al, 2013a in prep).
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The embryonic and tissue-specific stem cells are also used for:

Generation of a novel gene targeting technology for tissue-specific stem cells (Research
Collaboration Agreement, Regeneron Pharmaceuticals, NY, USA)

Screening of synthetic compounds involved in stem cell self-renewal and differentiation
(in collaboration with Profs. A. Gravanis, Univ. of Crete and L. Skaltsounis, Univ. of
Athens). (Patent Neurosteroid compounds, Bionature ltd., Pub. No.: WO/2008/15553,
24.12.2008).
Our future research will focus on:

Understanding the mechanisms of conversion of iTS in situ and endogenous tissuespecific stem cells into their progeny in situ and de novo (mouse and human) (Human
stem cell project in collaboration with Dr. Ron McKay, LIBD, Johns Hopkins Medical
School, Baltimore, USA)

The elucidation of the mechanism employed by Sox2 in EMT by means of a
conditional ablation (in collaboration with Dr. Aris Economides Regeneron
Pharmaceuticals, NY, USA and Prof. Pascal Dollé, IGBMC, Strasbourg, France) and
MET by means of Sox2-induced direct reprogramming.

Key epigenetic landscapes (Epi-G) and “epiribomic” determinants (Epi-R) of tissuespecific stem cells, generated by differentiation and reprogramming (Εpi-G in
collaboration with Prof. Marco Bianchi at DIBIT Stem Cell Institute, Milan; Epi-R in
collaboration with Dr. D. L. Kontoyiannis, II BSRC “Alexander Fleming”).
Understanding how a tissue-specific stem cell identity is acquired and maintained will enable
us to reveal the pathways involved in both organ regeneration, as well as tumour initiation
events and eventually lead to the design of non-invasive therapeutic protocols for in situ
tissue repair and cancer.
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