Product Information
WelTaq™ DNA Polymerase
Composition of 10X Reaction Buffer
100 mM Tris-HCl (pH8.3), 500 mM KCl, 15 mM MgCl2
Catalog Number ML 100-01
PCR Products
Storage Temperature -20oC
As most PCR products amplified with Taq have one A added
at 3'-termini, the obtained PCR product can be directly used for
cloning into T-vector. Also it is possible to clone the product in
Product Description
Taq DNA Polymerase is a thermostable DNA polymerase that
catalyzes the polymerization of nucleotides into duplex DNA in
blunt-end vectors after blunting and phosphorylation of the
end.
the 5’ → 3’ direction. WelTaq™ DNA Polymerase is isolated
from recombinant E.coli strain containing the Taq DNA
polymerase gene from Thermus aquaticus . It exhibits its
highest activity at pH 9.0 and 72°C.
Contents
General Reaction Mixture
WelTaq™ DNA Polymerase
0.5 ul
10X Reaction Buffer
dNTP Mixture (2.5 mM each)
5 ul
4 ul
Template
< 500 ng
WelTaq™ DNA Polymerase (5 units/ul)
500 units
Primer 1
0.2 - 1.0 uM (final conc.)
10X Reaction Buffer
1 ml
Primer 2
0.2 - 1.0 uM (final conc.)
10 mM dNTP Mixture (2.5 mM each)
0.5 ml
Sterilized Distilled Water
Up to 50 ul
Standard PCR Condition
Storage/Stability
WelTaq™ DNA polymerase, including buffers and reagents,
Step
Temp.
should be stored immediately upon receipt at -20°C. If stored
Predenaturation
94oC
1 min
in the recommended temperature, this product will be stable
Denaturation
94 oC
15 ~ 30 sec
until the expiration date printed out on the label.
Annealing
AT*
15 ~ 30 sec
Extension
72 oC
1 min/Kbs
Final extension
72 oC
1 ~ 5 min
Storage Buffer
20 mM Tris-HCl (pH8.0), 100 mM KCl, 1 mM DTT, 1 mM
Time
Cycles
1
25 ~ 35
1
* Annealing temperature (about 5°C below Tm of primers)
PMSF, 0.5% Nonidet P-40, 0.5% Tween 20, 50% Glycerol
Notes
Unit Definition
This product is for research use only. It is not intended for use
One unit is the amount of enzyme that will incorporate 10 nmol
in therapeutic or diagnostic procedures for humans or animals.
of dNTP into acid-insoluble products in 30 minutes at 74 oC.
Also, do not use this product as food, cosmetic, or household
item, etc..
Applications
・ For DNA amplification by Polymerase Chain Reaction
・ For DNA sequencing
WG-IFU-LM00107
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WelGENE is a specialized brand in the Cell Culture Industry