Structural Biology Services
Quotation Request Form
Please complete the form and submit to order@proteinct.com.
Our service representative will contact you shortly with a quote.
Page 1 of 3
Structural Biology Services
We offer two milestone based structure biology services:
CT-CrystalClear (X-ray crystallography) and CT-SAR-by-NMR (NMR spectroscopy)
For each service, we offer complete workflow, from gene synthesis, cloning, expression screening, purification, various necessary
steps prior to structure determination and ultimate to protein structure determination. Studies of protein complex can also be
included in the quotation.
Customer Information
If you have an existing account with ProteinCT, just fill in your name and email address or your Account No.
Name of Requestor:
Account No.:
Name of PI:
First:
Last:
Middle:
Title:
Organization:
Phone:
Fax:
Email Address:
Shipping Address
Project Information
Nature of Inquiry
Pricing estimation
Quote for ordering
Estimated Project
Initiation
Immediately
>6 months
1-3 months
3-6 months
Other, please specify
Research Use Only
Clinical Applications
Intended Use
For grant application purpose
Please note that we produce materials for research use only. We can perform developmental work for future
clinical applications, but are not GMP-certified.
Target information
Target:
Protein name:
Accession number:
Species:
Protein sequence:
MW:
Please indicate mutations/variants as compared to the sequence corresponding to the accession number.
Target Properties:
Service Packages:
Comments:
Membrane-bound
Secreted
Cytosolic
Nuclear
Periplasm
Protease
Other enzymes
IgG antibody
Toxic to E.coli
Other features to consider, please specify:
CT-CrystalClear: X-ray crystallography
CT-SAR-by-NMR: NMR spectroscopy
Both of above
Descriptions of each package are on Pages 2 and 3 of this quotation request form.
Please complete the form and submit to order@proteinct.com.
Our service representative will contact you shortly with a quote.
Structural Biology Services
Quotation Request Form
Page 2 of 3
Description of Structure Biology Service Packages
CT-Function™ CrystalClear Structure Service
This service is a milestone based structure determination service by X-ray crystallography. The progress of every project is
evaluated at each milestone point and communicated to the customer in a timely fashion. Delivery timeline is project
dependent.
Phase of Project
I.
Milestones
Cloning and
Expression
Optimization
Service Specifications
 Gene synthesis with codon
optimization
 Subclone into expression
vector(s)
 Expression screening
 Expression condition
optimization
Deliverables
 Report of fully optimized
sequence
 record of cloning and
expression screening
optimization
Scale-up protein
production

High purity protein >95% pure,
ready for crystallization screening
I. Protein production


Crystallization
Screening
II. Crystallization
Crystallization
optimization
II.
III.
X-ray data collection
III. Crystal structure
determination
Crystal structure
determination
Co-crystallization
IV. Protein complex
analysis(optional)
X-ray data collection
Crystal structure
determination
Protein expression at scales for
generating sufficient quantities
of target protein
Optimized purification for high
quality protein production
QC by SDS-PAGE

Crystal Screening HTP screening
under 1536 conditions and/or
 Manual screening using
standard kit analysis
Manual condition optimization to
generate diffraction crystals
Collection of high-resolution X-ray
data
Structure determination proprietary
automated crystallography software
and manual optimization

Soaking crystal with
compound(s)
 Co-crystallization of protein
complex
Collection of high-resolution X-ray
data
Structure determination with
crystallography softwares
Report of results under
crystallization conditions
Protein Crystal
X-ray data


Density map
protein structure ready for
PDB deposition
 Structure statistics
Protein complex crystal
X-ray data



Density map
Protein structure (PDB
format)
structure statistics
Please complete the form and submit to order@proteinct.com.
Our service representative will contact you shortly with a quote.
Structural Biology Services
Quotation Request Form
Page 3 of 3
Description of Structure Biology Service Packages
CT-Function™ SAR-By-NMR Service
This service is a milestone based structure determination service by NMR spectroscopy, Structure-Activity-Relationshipsby-NMR, with options for protein compound binding screenings. The progress of each project is evaluated at each
milestone point and communicated to the customer. Delivery timeline is project dependent.
Phase of Project
I. Protein
production
Milestones
Cloning and Expression
Optimization
15
N/13C Isotope labeling
and protein production
Folding screening by
15
N HSQC
NMR data collection
IV.
II. NMR structure
determination
Service Specifications
 Gene synthesis with codon
optimization
 Subclone into expression vector(s)
 Expression screening
 Expression condition optimization
 Protein expression at scales for
generating sufficient quantities of
15
N/13C Isotope labeled target
protein
 Optimized purification for high
quality protein production
 QC by SDS-PAGE
15

N HSQC screening for best data
collection conditions




NMR solution structure
calculation
III. Protein
dynamics, disorder,
stability analysis
(optional)
V.
IV. Protein Complex
determination and
functional analysis;
Protein-Protein ,
Protein / Nucleic
Acid complex
analysis (optional)
Backbone Assignments
Sidechain assignments
NOE-derived distance restraints
and J-coupling-derived dihedral
angle restraints
Residual dipolar couplings
Deliverables
 Report of fully optimized
sequence
 record of cloning and
expression screening
optimization
High purity 15N/13C Isotope
labeled protein >95% pure, ready
for NMR experiments
Report of screening conditions
and results, including 15N HSQC
spectra
NMR data
Structure ensemble
Data collection
Analysis of userspecified parameter
Structure determination with
proprietary automated and manually
adjusted software programs
Data collection
Determination of requested
parameters
Preparation of protein
complex


NMR data and report
Protein binding site
determination
Complex structure
determination
Titration of ligand
Determining best complex
conditions
 Chemical shift perturbation studies
/ Xfiltered NOESY anaylsis
 Structure analysis
Milestones and steps refer to phase I-III
NMR data
Detailed report of user-specified
parameters
Report of binding characteristics
including structure
representation
 Protein Structure ensemble
 Structure Analysis Report
 NMR data