A FIELD STUDY OF MAIZE YIELD ON MIXED ORGANIC AND CONVENTIONAL DAIRY FARMS IN DENMARK IN 2001 Jozef Sowinski1, Ib Sillebak Kristensen2, and John E Hermansen2 1 Department of Crop Production, Agricultural University of Wroclaw, Norwida 25 50-375 Wroclaw e-mail: sowinski@ekonom.ar.wroc.pl 2 Danish Institute of Agricultural Sciences, Department of Agricultural Systems Research Centre Foulum, P.O. Box 50, 8830 DK-Tjele e-mail: ibs.kristensen@agrsci.dk e-mail: john.hermansen@agrsci.dk Precented at conference: „Scientific aspects of organic farming“. 21-22. March 2002. Latvia University of Agriculture in Jelgava. SUMMARY A field study was carried out on mixed dairy farms in Denmark, five organic and five conventional farms. The surveys were set up to identify main reasons for low yields under practical conditions. Cob yield and quality of maize for silage as well as weeds were measured in 34 fields with five samples per field. Conventional maize gave a cob yield of 7,6251,922 Scandinavian Feed Units (SFU) ha-1, and organic maize 3,6421,813 SFU ha-1. The main yield components for the conventional and organic farms respectively were: Cob weight 83 and 49 g DM cob -1, dry matter in cobs: 42 and 32%; Maize plant number 80,000 and 66,000 plants ha-1. Other main differences were climatic differences between North and South Jutland, where North Jutland had 5% lower T-sum compared to South. On organic farms weeds were a problem, especially from midsummer until harvest, a period where weed grew vigorously under the light open maize. On the organic farms the weed content at harvest averaged 25% of the crop volume. Also, maize height was lower by 25% in organic fields, and 19% of the kernels of the cobs were unfilled, double of that of the conventional fields. Statistical models are given to quantify importance of the above factors. Key words: maize, organic and conventional farms, cob yield, Scandinavian Feed Unit, weed. INTRODUCTION With an average T-sum of 2,200-2,500 (Corn Heat Unit, (3) Denmark constitutes the northern border for maize production. Late varieties (T-sum more than 2,500) have to be harvested with low dry matter content in cold years. In variety trials the yields of recommended varieties averaged 11,300 1,500 SFU ha-1 in the years 1996-2000 measured from direct harvest of the plots (1). 1 SFU (Scandinavian Feed Unit) corresponds to the feeding value of 1 kg barley. On 10-16 private mixed dairy pilot farms a silage yield of 8,2002.100 SFU ha-1 was achieved in the years 1996-2000 (2). In year 2000, 5 mixed organic dairy farms had achieved only 4,7001,300 SFU ha-1. The silage net yield is measured when fed to the dairy herd, after field and silage losses. However a net silage yield was still only 40% of the plot yield achieved in field trials, indicating big losses. To investigate causes for low net yield under real life and practical conditions a survey was set up to quantify the field yield on organic and conventional mixed dairy farms. Document1 MATERIALS AND METHODS The investigation was carried out on 5 conventional and 5 organic farms. Farmers were chosen from a group of pilot farms of 30 farms cooperating with The Farming System Group in The Department of Agricultural Systems. From each field farm records were made on all treatments (sowing, spraying etc.). Midsummer the crop was evaluated in 3 subplots for plant density, weed competition and pest. Again 2-20 days before harvest the crop was evaluated, and cobs samples were taken. The farms are all located in Jutland. At harvest in each field five subplots were chosen along the longer diagonal of the field. Data were collected from a total of 170 subplots. Each subplot was 5 m long and 4 rows wide. Area of subplots for most fields was 15 m2 (in one field 10 m2). In each subplot the following measurements were carried out, in order to describe yield and quality of maize: Number of plants Number of developed cobs defined as cobs of more than half the normal size Height of plant (from 3-5 measurement per subplot) Number of missing plants (empty space) The cob's dry matter yield was estimated from number of plants per 15 m2 (~ 120 plants), weight of cobs (from 16 plants) and dry matter content in the cob (5 cobs). For the rest of cobs (11 cobs) development was evaluated. Percentage of cobs with poor or unfilled grains was evaluated as percentage of total cobs. Volume of weeds and volume of maize were evaluated in cubic meters per 15 m2. Calculated SFU yield in cobs were calculated in accordance to Danish standards (5), based on analyses of dry matter, crude protein, starch, crude fibre and in vitro organic matter digestible. 20 samples was analysed by conventional chemical methods and regression to NIR-analyses was used to estimate each sample. Statistical analyses were made at farm and field level using multiple linear regression methods (GLM procedure in SAS). Effects of system – organic vs. conventional farm – were tested by the following model: Model 1: Yijkl where Yijkl Systemi Farmj Fieldk Errorijkl = = = = = = Systemi + Farmj (Systemi)+ Fieldk (Farmj (Systemi)) + errorijkl SFU yield of cobs ha-1 for the individual plot within field Organic and conventional mixed dairy farms, i = 1-2. Farms within each system, j =1-5 Fields within each farm, k =1-8 Residual Systems were tested against Farm(System) and farms were tested against Field(Farm(System)). Afterwards the covariates were tested in model 2, where model 1 was extended with the covariates: Temperature sum, precipitation, day of sowing, weed in summer and autumn, earliness of variety, development stage at harvest, maize plants, % empty kernals. RESULTS AND DISCUSSION 2 The climate in year 2001 was favourable for maize growth. T-sum accumulated from 15. April to 15. October had a variation from 2420-2760 degree-day and was in average 7% higher than 20 years' average,(1). In June the maize was turning yellow during a 3 week cold and rainy period (100C). Some herbicide inhibition was observed during this period. In Table 1 the average treatment of the fields is described. Especially the conventional farms had selected a field with warm microclimate for continuous maize production for 2-6 years. Maize on organic farms had only been grown for 1-3 years. The total level of N-fertilisation was the same within the two groups. On organic farms a bigger proportion of animal manure were from deep litter, which only have low N-fertilizer value and a high K-content. On the conventional farms start fertilisers (around 30 kg N & 15 kg P ha-1) were placed 5-10 cm beside maize seeds. Table 1. Soil type, and fertilization in organic and conventional maize fields in DK 2001, mean + standard deviation. System Soil type1) - number of fields on sand + clay Organic manure (t per ha) Total amount of: - N2) -P -K - Mg -S 1) 2) Organic Conventional 7+ 3 44 17 17 + 7 50 13 147 +20 38 10 321 78 10 1 7 16 147 +19 47 8 185 54 18 5 11 5 Sandy = % clay < 10% and fine sand <40% (20-200 m), clay = % clay > 5% and fine sand >40% Animal N is calculated as artificial N-fertiliser equivalent In average the organic farmers were weed harrowing 5 times beginning with blind harrowing before maize could be seen above ground, 2-4 weed harrowing in May/June and ending with 13 row harrowing in June until maize height of 0.5 m. In conventional farms the maize were sprayed 2-3 times in average. Frit flies and weed were treated in average low doses was used with average 0.4 standard doses per treatment. In table 2 the characteristic of the maize fields is shown. On some of the organic farms false seedbeds were prepared 1-4 weeks before sowing in order to stimulate weed germination, which could be killed during seedbed preparation. This is probably the main reason for later sowing among the group organic farms. Eleven days later sowing among organic farms could explain lower T-sum from sowing to 1. August. The conventional fields had 70 mm higher precipitation however no drought occurred in year 2001. 3 Table 2. Characteristic of maize fields. Average + standard deviation. System Observation (fields) Day of sowing T-sum PrecFert_June,mm Plants_ha, 1000 ha-1 “Germination”2), % of sown WeedSummer, soil cover WeedHarvest, soil cover WeedHarvest, % vol. of maize Maize_m % empty cereals in cobs Pct. sideshoot % DM in cobs g DM cob-1 Cob-yield, 1000 kg DM ha-1 Cob-yield, 1000 SFU ha-1 Organic 50 (10) 8. May +10 1262 +89 82 +27 66 +151 66 +15 30 +15 59 +30 25 +181 1.7 +0.4 19 +14 1 +1 32 +7 49 +21 3.4 +1.7 3.6 +1.8 Conventional 120 (24) 28. April +9 1307 +61 153 +28 80 +121 89 +10 8 +9 40 +36 4 +61 2.3 +0.2 9 +8 5 +6 42 +8 83 +19 7.1 +1.7 7.6 +1.9 1) Mean of the standard deviation within fields 2) “Germination” = Full developed maize plants at harvest as percentage of sown seeds The number of maize plants in organic fields was only 66,000 varying from 51-81,000 maize ha-1. The recommended number in Denmark is 80-100,000 maize ha-1. The low maize number could be caused by lower germination and physical damage during weed harrowing in organic fields. Weed harrowing was difficult during June in period with slowly maize growth (10 0C in 3 week and raining) and a lot of weed survived in the maize rows. In warmer years this weed can be killed when covered by thin soil layer from weed harrowing, but when maize were not growing the small maize would also be covered. In average the organic fields had 30% weed cover in July and only 3 fields had less than 25% weed cover at harvest. After 1. July when weed harrowing were not longer possible the weed spread vigorous and at harvest the weed covered 59% soil in organic field and were tall, so 25% of the maize volume were covered by weed. In conventional fields the soil cover were also 40%, but the weed were low and accumulated to only 4% of the maize volume. In organic fields dominating weed were: Chenopodium album L. ssp. Album (20.8% soil cover), Elytrigia repens (L.) Nevski (5% soil cover), Solanum nigrum L. (4.2% soil cover), Stellaria media (L.) Vill. (3.1% soil cover). In conventional fields dominating weed were: Poa annua L. (17.4% soil cover), Elytrigia repens (L.) Nevski (5.5% soil cover), Solanum nigrum L. (5.2% soil cover). The organic maize were 0.6 m shorter than conventional and the cob-yield were lower because of 24% lower dry matter content in cobs, 41% lower cob weight and double proportion of empty cereals in cobs. Unfavourable conditions during later growth stages of maize growth may have reduced number of silks resulting in poor pollination and restricting the number of developed cereals (10). Weed probably is the main course of the low yield. In Table 3 the correlation between cob yield and main characteristics is shown. 4 Table 3. Correlations between cob-yield and covariates of 34 maize fields (n = 34). Cob-yield BBCH Plants_ha Height_m % EmptyCereals Volume weedHarv BBCH Plant_ha Height_m %EmptyCereals 0.7*** 0.7*** 0.3 -0.6*** -0.5** -0.2 -0.4* Earlines PrecFert_Ju PLN_ha 0.7*** 0.2 0.6*** ne Cob-yield BBCH Plants_ha Maize_m % EmptyCereals Volume weedHarv T-sum Earlines PrecFert_June PLN_ha Volume weedSummer Covariates at farm level: T-sum PrecFert_June Day of sowing WeedSummer Earlines BBCH Covariates at plot level: Plant_ha % empty kernals WeedHarv -0.1 0.0 0.2 0.0 -0.2 -0.2(*) -0.3 0.5** 0.1 0.6*** 0.7*** -0.2 -0.4** -0.1 0.0 0.0 0.0 0.0 -0.1 -0.4* 0.2 0.4* -0.4 0.1 Volume weedSummer -0.6*** -0.3(*) -0.5** -0.7*** 0.4* 0.6*** -0.1 -0.1 -0.6*** 0.2 = = = = = = T-sum from sowing to 1. August. (3) Accumulated precipitation from first fertilisation to 1. July Day number after 1. January 2001 of maize sowing. Soil cover of weed in July Earliness of each variety (5) Stage of development on harvest date, BBCH scale 75-85(6). = = = Number of maize plants ha-1 % unfilled kernals of total cob m3 weed per m3 maize just before harvest Volume weedHarv -0.5** -0.3 -0.2 -0.6*** 0.5** T-sum 0.5** 0.7*** 0.1 -0.2 -0.2 0.0 Day of sowing 0.6*** 0.7*** 0.2* 0.0 -0.3*** -0.2* 0.9*** -0.3*** 0.1 0.3*** -0.2* A correlation matrix based on the individual plots (n=170) showed the same overall picture as Table 3. The cob yield was positively correlated with development stage in the interval 75-85 BBCH; with maize plants ha-1; with maize height; with T-sum between sowing and 1. August; with precipitation and with day of maize sowing. The weed in midsummer (soil cover) and % empty cereals in cobs had negative correlation with cob-yield. However from the correlation matrix it can be seen that several of the covariates were correlated making the estimates in linear statistical analysis dependent. In the following only covariates with lower correlation than + 0.5 are included in the linear regression model at the same time. There was a significant effect of growing system and of farm within growing system on cob yield, the expected cob-yield being 7,656 SFU ha-1 in the conventional system and 3,709 SFU ha-1 in the organic system (Table 4). Farm 81-1 and 82-1 had lower yield than 40-1, 41-1 and 42-1. Explanation for this can be that 81-1 and 82-1 were situated in North Jutland. The fields were sown 10-20 days later in the same period as the organic fields, also in North Jutland 44 mm extra surplus precipitation in June could have leached some N out of the root zone, at least the maize turned yellow in June because of cold rainy weather during 3 weeks (100C and up to 100 mm precipitation). 5 Table 4. Analysis of variance of maize cob-yield (SFU ha-1) on five organic and five conventional mixed dairy farms in year 2001 and LS-mean cobs for system and farm. Variable DF System 1 Farm (System) Field (farm (system)) Residual Organic Conventional Farms Organic 30-9 31-9 62-9 63-9 35-5 Conventional 40-1 41-1 42-1 81-1 82-1 Sum of square Mean square 560 8 227 24 230 136 144 Estimates for cob yield 3,709 7,656 560 Pr > F R2 0.003 0.88 28 0.019 9 0.0001 1 Standard Error 166 100 3,387 3,688 4,746 3,426 3,299 325 231 461 326 461 7,466 9,748 8,815 6,610 5,642 163 231 231 231 231 In order to investigate how much of the variation in cob yield between fields that could be explained by the measured state variates, maize plants per ha, weed, earliness of variety, T-sum from sowing to 1. August, precipitation from fertilization to 1. June, N-application, maize plant height, maize stage of development at harvest were includes as co-variates. Only co-variates with interdependence lower than +0,5 correlation were included in the model at the same time (Table 3). Table 5. Analysis of variance of maize cob-yield (SFU ha-1) on 5 organic and 5 conventional mixed dairy farms in year 2001. Variable Plants_ha Weedharv T-sum Residual DF 1 1 1 30 Sum of square 64 65 42 32 Mean square 64 65 42 1 Estimate 1.05 -82 -43 Pr > F < 0.0001 < 0.0001 < 0.0001 R2 0.84 From Table 5 the significant variables of above mentioned are shown. This difference in cobyield of 4000 SFU ha-1 between organic and conventional fields could be explained by maize plants ha-1 and weed volume at harvest. A difference of 14,000 plants ha-1 (80,000-66,000 plants in conventional and organic fields, see Table 2) could explain 1,469 cob SFU ha-1 (= 14,000 x 1.05). Weed difference of 21% of maize volume could explain 1,721 SFU ha-1 (21 * 82) and Tsum 726 SFU ha-1. In figure 1 the relationship between SFU and predicted cob yield by the model in Tabel 5 are shown. Symbols are shown for system, altitude (north/south) and fields with higher than 25% weed soil cover mid summer (=weedy). From the figure a tendence can be seen for stronger relationship between measured and predicted yields in conventional fields compared with organic fields indicating that the yield potential are better utilized in clean conventional fields compared to weedy organic fields. 6 Excluding environmental variables, yield losses in maize are caused mainly by competition from weeds (9, 11). Importance of weed competition is described for critical periods. For maize the critical period ranges from 1 to 8 weeks after maize emergence (4, 8, 11, 12). Weed competition in early growing stage was the main problem and depressed yield of cobs on organic farms. Focus should also be paid to weed control especially in the maize rows. From trial results it should be possible to control weed better than 15-45% weed soil cover midsummer. But between 30-cm maize height and harvest it can be difficult to control weed competition. So fields with low seed bank of weeds should be selected for maize production even through quickly weed enrichment should be expected in these fields. Weed competition and probably nutrient uptake by weeds were related to a high percentage of empty cobs in organic group. Probably an effect of weather conditions (temperature at early growth stages of maize) or wrong types of varieties where main indicators of lower yield of maize cobs from the northern part of Denmark. CONCLUSIONS Maize cob yield in 24 conventional and 10 organic fields in year 2001 could be predicted from maize density, weed and T-sum (R2 = 84%). Weed volume at harvest explaining 44% of the yield difference between the group of organic and conventional fields; maize number per ha 37% and the T-sum between sowing and 1. August 18% of the yield difference. 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