Objectives – Transcription
1. Define gene expression.
2. Define and describe the molecular components of a typical gene.
3. How do DNA and RNA differ from one another?
4. Describe the RNA polymerase holoenzyme of E. coli.
5. What are the components of a prokaryotic promoter? What are the functions of a
promoter? Understand what is meant by Upstream and Downstream from a Promoter.
6. Describe the process of transcription (in prokaryotes) be sure to include events at
initiation, elongation and termination.
7. During transcription, which strand is the sense strand and which strand is the non-sense
strand? What do these two terms mean?
8. Differentiate between rho-independent and rho-dependent termination of transcription
in prokaryotes.
9. What is Abortive Initiation?
10. Describe the different classes of RNAs found in a cell.
11. What are the components of a eukaryotic promoter? What are the functions of a
promoter? Understand what is meant by Upstream and Downstream from a Promoter.
What are UASs?
12. What are the functions of RNA polymerase I, II and III?
13. What is a consensus sequence?