Appendix S1 Supporting Tables and Figures Table S1 Information for all lakes involved in the experiment. Data was collected from May 2012 to August 2012 (SE Arnott, unpublished data). Lake Opinicon was the source of all mesocosm water and zebra mussels, while zooplankton and phytoplankton samples were taken from the other five lakes. Lake Elbow Lindsay Long Opinicon Round Warner Latitude (N) Longitude (W) 44°28'37.0" -76°25'40.8" 44°32'14.2" -76°23'25.1" 44°31'40.5" -76°24'10.0" 44°33'35.4" -76°19'41.0" 44°32'16.9" -76°24'00.3" 44°31'41.4" -76°22'53.0" Area (ha) Max Depth (m) pH DOC -1 (mg L ) Cond. Av. TP (μS cm ) (μg L-1) -1 15.5 10.6 7.69 7.50 77.0 11.7 31.5 10.9 8.09 6.65 230.5 8.6 15.5 26.0 8.23 4.30 182.0 5.0 788.0 11.3 8.17 6.37 192.0 13.3 15.0 30.1 8.14 4.00 164.0 6.0 9.2 6.4 8.03 7.05 246.0 10.4 Table S2 Full species names, abbreviations, size group, habitat within a lake, and diet for all zooplankton important to differentiating between communities in Figure 4. Other zooplankton were present (e.g. Daphnia spp.), but were either ubiquitous over all communities, or not abundant enough to be useful for differentiation. Species Abbreviation Acroperus harpae Alona rectangula Alona guttata Alona quadrangularis Bosmina liederi/freyi Camptocercus rectirostris Ceriodaphnia quadrangula Chydorus sphaericus Graptoleberis testudinaria Pleuroxus denticulatus Pleuroxus truncatus Polyphemus pediculus Calanoid copepodid Cyclopoid copepodid Calanoid nauplii Cyclopoid nauplii Cyclops scutifer Cyclops vernalis Mesocyclops edax Keratella cochlearis Keratella serrulata Lecane inermis Lecane mira Monostyla copeis Monstyla closterocerca Mytilina ventralis Polyartha remata Trichocerca rattus Trichocerca pusilla A.h. A.r. A.g. A.q. B.l/f. C.r. C.q. C.s. G.t. P.d. P.t. P.p. Ca.c. Cy.c. Ca.n. Cy.n. Cy.s. Cy.v. M.e. K.c. K.s. L.i. L.m. M.co. M.cl. M.v. P.r. T.r. T.p. Group Size Habitat Diet Cladoceran Cladoceran Cladoceran Cladoceran Cladoceran Cladoceran Cladoceran Cladoceran Cladoceran Cladoceran Cladoceran Cladoceran Copepod Copepod Copepod Copepod Copepod Copepod Copepod Rotifer Rotifer Rotifer Rotifer Rotifer Rotifer Rotifer Rotifer Rotifer Rotifer Small Small Small Small Small Small Small Small Small Small Small Small Juvenile Juvenile Juvenile Juvenile Small Large Large Small Small Small Large Small Small Large Small Large Large Littoral Littoral Littoral Littoral Pelagic Littoral Both Littoral Littoral Littoral Littoral Pelagic Herbivorous Herbivorous Herbivorous Herbivorous Herbivorous Herbivorous Herbivorous Herbivorous Herbivorous Herbivorous Herbivorous Carnivorous Herbivorous Herbivorous Herbivorous Herbivorous Omnivorous Carnivorous Omnivorous Herbivorous Herbivorous Herbivorous Herbivorous Herbivorous Herbivorous Herbivorous Herbivorous Herbivorous Herbivorous Both Both Both Pelagic Pelagic Littoral Littoral Littoral Littoral Littoral Pelagic Littoral Littoral Figure S1 Change in community location (represented by PCoA Axis 1 and 2 scores) with increasing nutrients for each mussel treatment. Best fit lines are shown in the treatments whose PCoA location is correlated with nutrient level and only when P < 0.1. Note log scale used on y-axes. Figure S2 Relationship between nutrient treatment and abundance (abund.) or concentration of rotifers (Rot.), copepods (Cop.), cladocerans (Clad.) and large phytoplankton (L. phyto., measured as total minus edible chlorophyll a) in the 0.0, 0.25, 0.5 and 1.0 mussels L-1 treatment densities. Best fit lines are shown in the treatments whose abundance or concentration is significantly correlated to nutrient level (P < 0.05). Note the break in the y-axis for rotifer abundance and the log-scale on the y-axis for large phytoplankton concentration.