Curriculum Vitae
Gregory W. Broussard
The Pennsylvania State University
Biochemistry and Molecular Biology
122B South Frear
University Park, PA 16802
Wk: (814) 863-0502
gwb13@psu.edu
Employment
Lecturer
The Pennsylvania State University
Biochemistry and Molecular Biology
2014-present
Research Associate
University of Pittsburgh, Pittsburgh, PA
Department of Biological Sciences
Laboratory: Dr. Graham F. Hatfull
2011-2014
Education/Training
Postdoctoral Training
University of Pittsburgh, Pittsburgh, PA
Department of Biological Sciences
Laboratory: Dr. Graham F. Hatfull
2007-2011
Ph.D. (Biology)
University of Louisiana, Lafayette, LA,
Department of Biology
Laboratory: Dr. Don G. Ennis
2001-2007
B.S. (Microbiology)
University of Louisiana, Lafayette, LA
Department of Biology
Minors: Chemistry and Psychology
1996-2001
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Course Teaching Experience
Courses Taught at Penn. State:
Medical Microbiology Laboratory (MICRB 422): Spring 2015
Laboratory demonstrating properties and classification of medically important
microorganisms and techniques used in their identification.
Laboratory in Molecular Immunology (MICRB 447): Spring 2015
Laboratory in molecular techniques to assay antigens, antibodies and receptor sites.
Introductory Microbiology Laboratory (MICRB 202): Spring 2015
(Lab coordinator of seven sections)
Qualitative and quantitative techniques with regard to recognition of bacteria and their
processes on a microscopic, colonial, and physiological basis.
Laboratory of General and Applied Microbiology (MICRB 421W): Fall 2014
Laboratory demonstrating fundamental techniques and principles of experimentation of
general and applied microbiology.
Elementary Microbiology (MICRB 106): Fall 2014
Importance of microorganisms in health and disease, agriculture, and industry;
descriptive course for students not planning advanced study in microbiology.
Courses Taught at Other Univesities: (During Graduate School)
Media Preparation, University of Louisiana: Fall 2002, Spring 2003, Spring 2004
Developed syllabus, lectured, wrote exams, graded exams, and instructed laboratory
work. Course involved an in depth knowledge of the components of different media
commonly used in a clinical microbiology laboratory setting. Media and solutions
produced were used to supply concurrent microbiology courses.
Molecular and Cellular Engineering Laboratory, University of Louisiana: Fall 2003
Instructed eleven graduate students and one undergraduate. Developed syllabus,
lectured, wrote exams, graded exams, and instructed laboratory work. Course involved
molecular techniques, including: manipulation of DNA, transformation of bacteria,
Southern and Western Blots, and protein purification, among others.
Individual Contact Teaching Experience
Phagehunter Program, University of Pittsburgh, 2007-2014
Participated in the phagehunter program, in the laboratory of Dr. Graham Hatfull, aimed
at education in science focused on undergraduates and high school students. Oversaw
journal club meetings and participated in general meetings.
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Undergraduate Research Instruction, University of Pittsburgh, 2007-2014
Mentored ten undergraduates conducting individual research projects over the course of
my post-doctoral and research associate career in Dr. Graham Hatfull’s lab. Projects
included phage discovery, genome sequencing, and exploring the functional genomics of
various aspects of phage biology. Within this group, individuals published results in
scientific journals, entered graduate school and entered medical school.
University of Louisiana: 2001-2007
Mentored eleven undergraduates over the course of my graduate career on laboratory
research concerning aspects of my dissertation. Undergraduates engaged in all aspects of
the laboratory, including lab meetings, discussion of research goals, and problem solving
strategies. Experiences involved aspects of bacteriology, ichthyology, pathology,
immunology, molecular biology, and disease modeling.
Instructed five undergraduates in a research project that resulted in the publication of a
journal article: Civilian household bioterrorism decontamination agents. Journal of
Undergraduate Chemistry Research 4: 165-168.
Research Interests
Phage functional genomics; phage-host interactions; bacterial pathogens, especially M.
tuberculosis and Vibrio spp.
Awards, Grants, Fellowships
NIH training grant in Molecular Microbial Persistence and Pathogenesis, 2008-2010
Award included 2 yrs. postdoctoral stipend and travel to scientific meetings
Award for Oral Presentation, 2006
Wind River Conference on Prokaryotic Biology
Award: Book, Gram Positive Pathogens, 2nd Ed., ASM Press
Three Ravin-Rogers travel award, 2003, 2004, 2006
Wind River Conference on Prokaryotic Biology
Total award: $800
AAAS/Science Program for Excellence in Science, 2006
Award included free membership to AAAS and free subscription to Science.
Ten UL Graduate Student Organization Travel Grants, 2001-2006
Grants awarded for travel to various meetings throughout graduate career
Total award: $2,400.00
Broussard, G.
Fourteen UL Graduate Student Organization Supply Grants, 2001-2006
Grants awarded for research supplies throughout graduate career
Total award: $2,240.00
ULL Fellowship, 2001-2005
Tuition and stipend during graduate school
Duties included two years of teaching responsibility and presentations at
departmental seminars
Award for Oral Presentation, 2005
Department of Biology Graduate Student Symposium
University of Louisiana
Professional Reviewer Activities
Associate Faculty Member, Faculty of 1000, 2012-2014
Manuscript Co-Reviewer, Tuberculosis, 2013
Manuscript Reviewer, Journal of Aquatic Animal Health, 2003
Invited/Visiting Scientist Activities
China Agricultural University, November 18th to December 2nd 2012
Collaborative work on recombineering in M. bovis at the College of Veterinary
Medicine.
Professional Memberships
American Society for Microbiology, 2001-2014
The American Association for the Advancement of Science, 2006-2014
American Society for Microbiology, Allegheny Branch, 2008-2010, 2012-2014
International Society for Viruses of Microbes, 2012-2013
Phages for Human Application Group Europe, 2012
Aquatic Models Society, 2006-2008
American Society for Microbiology, South Central Branch, 2001-2007
American Society for Microbiology, Texas Branch, 2001-2003
GenBank Entries
Mycobacterium phage Acadian, complete genome, accession number JN699007
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Publications
Peer-Reviewed Publications
1. Pope, W.H, Broussard, G.W. (6th of 41 authors), et. al., 2013. Mycobacteriophages
Rey, PegLeg and Bongo constitute the new Cluster M. Journal of Virology.
(submitted)
2. Pope, W.H., Jacobs-Sera, D., Best, A.A., Broussard, G.W., et al., 2013. Cluster J
mycobacteriophages: Intron splicing in capsid and tail genes. PLOS One 8(7),
e69273.
3. Silva, J., Piuri, M., Broussard, G., Marinelli, L., Bastos, G., Hirata, R., Hatfull, G.,
Hiratta, M., 2013. Application of BRED technology to construct recombinant D29
reporter phage expressing EGFP. FEMS Microbiology Letters 344, 166-172.
4. Broussard, G.W. and Hatfull, G.F., 2013. Evolution of genetic switch complexity.
Bacteriophage 3(1), e24186.
5. Broussard, G.W., Oldfield, L., Villanueva, V., Lunt, B., Shine, E., Hatfull, G.F.,
2013. Integration-dependent bacteriophage immunity provides insights into the
evolution of genetic switches. Molecular Cell 49(2), 237-248.
6. Jacobs-Sera, D., Marinelli, L., Bowman, C., Broussard, G., Guererro, C., Boyle, M.,
Petrova, Z., Dedrick, R., Pope, W., SEA-PHAGES PROGRAM; Modlin, R., Hendrix,
R., Hatfull, G., 2012. On the nature of mycobacteriophage diversity and host
preference. Virology 434, 187-201.
7. Traag, B.A., Driks, A., Stragier, P., Bitter, W., Broussard, G., Hatfull, G., Chu, F.,
Adams, K.N., Ramakrishnan, L., Losick, R., 2010. Do mycobacteria produce
endospores? PNAS 107, 878-881.
8. Sampson, T., Broussard, G.W., Marinelli, L.J., Jacobs-Sera, D., Ray, M., Ko, C-C.,
Russel, D., Hendrix, R.W., Hatfull, G.F., 2009. Mycobacteriophages BPs, Angel and
Halo: comparative genomics reveals a novel class of ultra-small mobile genetic
elements. Microbiology 155, 2962-2977.
9. Broussard, G.W., Norris, M.B., Schwindt, A.R., Fournie, J.W., Winn, R.N., Kent,
M.L., Ennis, D.G., 2009. Chronic Mycobacterium marinum infection acts as a tumor
promoter in Japanese medaka. Comparative Biochemistry and Physiology, Part C
149, 152-160.
10. Broussard, G.W., and Ennis, D.G., 2007. Mycobacterium marinum produces longterm chronic infections in medaka: a new animal model for studying human
tuberculosis. Comparative Biochemistry and Physiology, Part C 145, 45-54.
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11. Flaherty, D., Guidry, L.A., Guilbeau, K.M., Lewis, A.M., Welch, C.A., Trahan, A.,
Broussard, G.W., Antley, P., and Taylor, E.R. 2004. Civilian household
bioterrorism decontamination agents. Journal of Undergraduate Chemistry Research
4: 165-168.
Dissertation
Broussard, G.W. 2007. Development and Application of Medaka and Mycobacterium
marinum as an Infection Model for Human Tuberculosis. (Doctoral Dissertation)
University of Louisiana, Lafayette, Louisiana.
Presentations
Invited Oral Presentations:
1. Broussard, G.W., Oldfield, L., Villanueva, V., Lunt, B., Shine, E., Hatfull, G.F.,
2012. Integration-dependent bacteriophage immunity provides insights into the
evolution of genetic switches. Presentation to the Student Body at the College of
Veterinary Medicine, China Agricultural University.
2. Broussard, G.W., 2012. Discovery and exploitation of mycobacteriophages. China
Institute of Veterinary Drug Control, Center for Veterinary Drug Evaluation, MOA.
3. Broussard, G.W., Oldfield, L., Villanueva, V., Lunt, B., Shine, E., Hatfull, G.F.,
2012. Integration-dependent bacteriophage immunity provides insights into the
evolution of genetic switches. Chinese Academy of Sciences.
Conference Oral Presentations:
1. Broussard, G.W., Oldfield, L.M., Villanueva, V.M., Hatfull, G.H., 2012. A novel
self-contained genetic switch. Pittsburgh Bacterial Meeting, Duquesne University.
2. Broussard, G.W., Oldfield, L.M., Villanueva, V.M., Hatfull, G.H., 2011. A novel
self-contained genetic switch. First Annual Postdoctoral Research Symposium, Dept.
Bio. Sci., Univ. Pittsburgh.
3. Broussard, G.W., Oldfield, L.M., Villanueva, V., Hatfull, G.F., 2010. Integration is
a prerequisite for mycobacteriophage BPs lysogeny establishment: a novel regulatory
mechanism for genetic networks. Pittsburgh Bacterial Meeting, Duquesne
University.
4. Mutoji, N.K., Wipple, C., Broussard, G.W., Guidry, L., Durling, L., Ennis, D.G.,
2008. Exploration into potential modes of transmission of Mycobacterium marinum
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in Japanese medaka (Oryzias latipes). The Annual Meeting of the Wind River
Conference on Prokaryotic Biology.
5. Broussard, G.W., Zaunbrecher, L.K., Norris, M.B., Winn, R.N., Fournie, J.W.,
Schwindt, A.R., Kent, M.L., Ennis, D.G.*, 2008. Evidence for increased cancer risks
with chronic mycobacterial infections. The Annual Meeting of the Wind River
Conference on Prokaryotic Biology. (Presented by Ennis, D.G.)
6. Broussard, G.W. and Ennis, D.G. 2006. Development of a Tuberculosis Model
Using the Small Fish Japanese Medaka and Mycobacterium marinum: Application of
See-Through Medaka. The Annual Meeting of the Wind River Conference on
Prokaryotic Biology.
7. Broussard, G.W. and Ennis, D.G. 2006. Mycobacterium marinum produces longterm chronic infections in medaka: a new animal model for studying human
tuberculosis. American Society for Microbiology, South Central Branch Meeting.
8. Broussard, G.W. and Ennis, D.G. 2005. Development of a tuberculosis model
using the small fish medaka (Oryzias latipes) and Mycobacterium marinum. Sixth
Annual Department of Biology Graduate Student Symposium, University of
Louisiana.
9. Ennis, D.G. and Broussard, G.W. 2005. Establishing medaka as a small fish model
for Mycobacterium marinum infections. The Annual Meeting of the Wind River
Conference on Prokaryotic Biology.
10. Broussard, G.W. and Ennis, D.G. 2005. Development of a tuberculosis model
using the small fish medaka (Oryzias latipes) and Mycobacterium marinum. Annual
Eastern Fish Health Workshop.
11. Ennis, D.G. and Broussard, G.W. 2005. Establishing medaka as a small fish model
for Mycobacterium marinum infections. Aquatic Animal Models of Human Disease
Conference.
12. Broussard, G.W. and Ennis, D.G. 2004. Establishing medaka as a small fish model
for Mycobacterium marinum infection. Fifth Annual Department of Biology
Graduate Student Symposium, University of Louisiana.
13. Broussard, G.W. and Ennis, D.G. 2004. Roles of Mycobacterial DNA repair
networks in fish pathogenesis. University of Louisiana, Biology Department
Seminar.
14. Broussard, G.W. and Ennis, D.G. 2004. Establishing medaka as a small fish model
for Mycobacterium marinum infections. The Annual Meeting of the Wind River
Conference on Prokaryotic Biology.
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15. Broussard, G.W., Zaunbrecher, A., Durling, L., and Ennis, D.G. 2004. Establishing
medaka as a small fish model for Mycobacterium marinum infections. American
Society for Microbiology, South Central Branch Meeting.
16. Broussard, G.W. and Ennis, D.G. 2003. Using small fish models to detect
mutagenesis induction by chronic inflammation. University of Louisiana, Biology
Department Seminar.
17. Ennis, D.G. and Broussard, G.W. 2003. Establishing medaka (Oryzias latipes) as a
small fish model for Mycobacterium marinum infections. Aquatic Animal Models of
Human Disease Conference.
Poster Presentations:
1. Cathcart, A., Broussard, G.W., Hatfull, G.F., 2013. Characterization of highly
conserved promoter-associated sequences in Cluster B mycobacteriophages.
University of Pittsburgh, Science 2013 Meeting.
2. Oldfield, L.M., Broussard, G.W., Villanueva, V.M., Hatfull, G.F., 2012. Control of
the BPs lytic-lysogenic genetic switch by divergent promoters. University of
Pittsburgh, Science 2012 Meeting.
3. Oldfield, L.M., Broussard, G.W., Villanueva, V.M., Hatfull, G.F., 2012. Control of
the BPs lytic-lysogenic genetic switch by divergent promoters. University of
Pittsburgh, Department of Biological Sciences Retreat at PLE.
4. Oldfield, L., Broussard, G., Villanueva, V., Hatfull, G., 2012. Gene expression of
mycobacteriophage BPs. Viruses of Microbes Meeting.
5. Villanueva V., Broussard, G., Oldfield L., Hatfull, G., 2012. Characterization of a
novel phage repressor. Viruses of Microbes Meeting.
6. Broussard, G., Oldfield, L., Villanueva, V., Shine, E., Lunt, B., Hatfull, G., 2012. A
novel lysogenic genetic circuit. Viruses of Microbes Meeting.
7. Villanueva, V.M., Broussard, G.W., Hatfull, G.F., 2011. The unusual immunity
system of mycobacteriophage BPs. American Society for Microbiology General
Meeting.
8. Oldfield, L.M., Broussard, G.W., Hatfull, G.F., 2011. Activation of the
mycobacteriophage BPs repressor by integration: a novel phage regulatory
mechanism. American Society for Microbiology General Meeting.
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9. Schmidt, L., Broussard, G., Jacobs-Sera, D., Hatfull, G., 2011. Mycobacteriophage
DaVinci reveals a genomic switch to a partitioning system for prophage integration.
American Society for Microbiology General Meeting.
10. Broussard, G.W., Oldfield, L.M., Villanueva, V., Sampson, T., Hatfull, G.F., 2010.
Activation of the mycobacteriophage BPs repressor by integration: a novel phage
regulatory mechanism. American Society for Microbiology General Meeting.
11. Broussard, G.W., Bowman, C., Hatfull, G.F., 2009. Mycobacteriophage lsr2 genes:
why have them? Pittsburgh Bacterial Meeting.
12. Broussard, G.W., Bowman, C., Cresawn, S., and Hatfull, G.F., 2008. May I
complement your Lsr2: Why do mycobacteriophage have Lsr2 homolgues?
University of Pittsburgh’s Science 2008 Meeting.
13. Broussard, G.W., Bowman, C., Cresawn, S., and Hatfull, G.F., 2008. May I
complement your Lsr2: Why do mycobacteriophage have Lsr2 homolgues?
University of Pittsburgh, Department of Biological Sciences Symposium.
14. Broussard, G.W. and Hatfull, G.F., 2008. Investigation into the ability of
mycobacteriophage lsr2 homologues to complement a lsr2 defect in Mycobacterium.
American Society for Microbiology General Meeting.
15. Mutoji, N.K., Broussard, G.W., Ennis, D.G., 2008. Biofilm formation and its effect
on virulence of Mycobacterium marinum in medaka (Oryzias latipes). The Annual
Meeting of the Wind River Conference on Prokaryotic Biology.
16. Broussard, G.W. and Ennis, D.G., 2006. Mycobacterium marinum produces longterm chronic infections in medaka: a new animal model for studying human
tuberculosis. American Society for Microbiology, South Central Branch Meeting.
17. Mutoji, N.K., Broussard, G.W., Guidry, L., Durling, L., and Ennis. D.G., 2006.
Natural modes of transmission of Mycobacterium marinum in medaka (Oryzias
latipes). American Society for Microbiology, South Central Branch Meeting.
18. Broussard, G.W. and Ennis, D.G., 2005. Development of a tuberculosis model
using the small fish medaka (Oryzias latipes) and Mycobacterium marinum. Aquatic
Animal Models of Human Disease Conference.
19. Mutoji, N.K., Broussard, G.W., Guidry, L., Durling, L., and Ennis, D.G., 2005.
Natural modes of transmission of Mycobacterium marinum in medaka (Oryzias
latipes). Aquatic Animal Models of Human Disease Conference.
20. Broussard, G.W., Brown, Y., and Ennis, D.G. 2003. Using the small fish model
medaka in an effort to link chronic infection and mutagenesis. The Annual Meeting
of the Wind River Conference on Prokaryotic Biology.
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21. Broussard, G.W., Granados, L.S., and Ennis, D.G. 2002. Studying mycobacterial
infection using small fish models and M. marinum expressing Gfp. The Fourth
International Symposium on Aquatic Animal Heath.
22. Broussard, G.W., Granados, L.S., and Ennis, D.G. 2002. Studying mycobacterial
infection using small fish models and M. marinum expressing Gfp. American Society
for Microbiology, South Central Branch Meeting.
23. Granados, L.S., Lemoine, F.J., Marriott, S.J., Higginbotham, R.H., Broussard, G.W.,
and Ennis, D.G. 2002. Effect of HTLV-1 Tax expression on spontaneous and UVinduced mutagenesis. American Society of Microbiology, South Central Branch
Meeting.