Table S1. Data sources and codes. The data used in fourth corner analyses are listed by matrix and then the trait category, code for each trait, the trait or character, and the source of the data. Sources include: TVA = Tennessee Valley Authority, mTVA = scores from the modified EPA level 1 habitat assessments developed by TVA, RK = surficial lithology shapefiles available through the USGS Mineral Resources Division On-Line Spatial Database, FT = FishTraits (Frimpong and Angermeier, 2009), TN = Etnier and Starnes (1993), RTM = Hollingsworth et al. (2013), NHD = United States Geological Survey (USGS) National Hydrological Dataset plus for the Tennessee River, NLCD = USGS National Land Cover Dataset. Category Code species Unique for each species Fish Functional Trait X Locality fd_benthic Benthic feeder fd_surwcol Surface/water column feeder algphyto Algae/phytoplankton feeder macvascu Macrophyte/plant feeder detritus Detritus feeder invlvfsh Invertebrates/larval fish feeder fshcrcrb Fish/crayfish etc. feeder blood Parasitic blood feeder eggs Egg feeder TL1-15 Max. total length 1-15 cm TL16-45 Max. total length 16-45 cm TL46-300 Max. total length 46-300 cm Mature1_2 1-2 years old at maturation Mature3mr 3 or more years at maturation Longevity1_4 Typical longevity in wild 1-4 years Longevity5-10 Typical longevity in wild 5-10 years Longevity11mr Typical longevity in wild 11 or more years Fec1k Reported fecundity under 1000 Fec10k Reported fecundity 1000-10,000 Fec100k Reported fecundity 10,000-100,000 Fec100kmr Reported fecundity more than 100,000 serial Serial spawning jan Spawns during January feb Spawns during February mar Spawns during March apr Spawns during April may Spawns during May jun Spawns during June jul Spawns during July aug Spawns during August sep Spawns during September oct Spawns during October nov Spawns during November dec Spawns during December Brdcst_opn Broadcast spawning with no concealment of feeding feeding feeding feeding feeding feeding feeding feeding feeding growth growth growth reproduction reproduction growth growth growth reproduction reproduction reproduction reproduction reproduction reproduction reproduction reproduction reproduction reproduction reproduction reproduction reproduction reproduction reproduction reproduction reproduction reproduction Trait Fish X Locality Individual species Source TVA FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT FT reproduction Brdcst_hid reproduction Cavity_nongd reproduction Guarder_nest reproduction Guarder_cav classification listed life habit life habit life habit classification life_Benthic life_midwtr life_topwtr Invasive physical trait physical trait physical trait physical trait habitat trait habitat trait habitat trait habitat trait habitat trait habitat trait habitat trait habitat trait habitat trait surficial geology surficial geology surficial geology surficial geology surficial geology surficial geology surficial geology surficial geology surficial geology surficial geology surficial geology surficial geology surficial geology eggs Broadcast spawning with at least minimal concealment of eggs Eggs deposited in cavities without subsequent parental care At least one parent guards a prepared nest area containing eggs At least one parent guards a cavity containing eggs Federal or State listing as imperiled to some degree Non-feeding position is benthic Non-feeding position is mid-water/pelagic Non-feeding position is top-water An established non-native species to the upper Tennessee River Environmental Characters X Locality Slope Slope of the stream segment containing the locality Sinuosity Sinuosity of the stream segment containing the locality Elevation Elevation at the locality DrainArea Drainage area of the watershed upstream of the locality BankStabil Average of bank stability scores BankVeg Average of bank vegetation scores ChanAlt Degree of channel alteration score Embed Proportion of fine sediment filling interstitial spaces of substrate EpiSub Quantity of epifaunal (aquatic insect) habitat RiffFreq Frequency of riffles at locality Cover Instream cover for fish RipZone Riparian vegetative zone quality SedDep Sediment deposition limestone Primary or Secondary surficial geology shale Primary or Secondary surficial geology dolomite Primary or Secondary surficial geology mudstone Primary or Secondary surficial geology sandstone Primary or Secondary surficial geology chert Primary or Secondary surficial geology siltstone Primary or Secondary surficial geology gneiss Primary or Secondary surficial geology conglomerate Primary or Secondary surficial geology claystone Primary or Secondary surficial geology arkose Primary or Secondary surficial geology amphibolite Primary or Secondary surficial geology schist Primary or Secondary surficial geology FT FT FT FT FT and state agencies TN, RTM TN, RTM TN, RTM TN and state agencies NHD NHD NHD TVA mTVA mTVA mTVA mTVA mTVA mTVA mTVA mTVA mTVA RK RK RK RK RK RK RK RK RK RK RK RK RK surficial geology surficial geology surficial geology surficial geology surficial geology surficial geology land cover metasedimentary slate marble quartzite phyllite migmatite OpH2O land cover DevOp land cover DevLow land cover DevMed land cover DevHi land cover Barren land cover DecForest land cover EvGForest land cover MixForest land cover ShrubScrub land cover GrassHerb land cover PastureHay land cover Crops land cover WoodyWetlands land cover EmHerbWetlands Primary or Secondary surficial geology Primary or Secondary surficial geology Primary or Secondary surficial geology Primary or Secondary surficial geology Primary or Secondary surficial geology Primary or Secondary surficial geology Percentage of contributing area classified as Open Water Percentage of contributing area classified as Developed Open Percentage of contributing area classified as Developed Low Percentage of contributing area classified as Developed Medium Percentage of contributing area classified as Developed Hi Percentage of contributing area classified as Barren Land Percentage of contributing area classified as Deciduous Forest Percentage of contributing area classified as Evergreen Forest Percentage of contributing area classified as Mixed Forest Percentage of contributing area classified as Shrub/Scrub Percentage of contributing area classified as Grasslands/Herbaceous Percentage of contributing area classified as Pasture/Hay Percentage of contributing area classified as Cultivated Crops Percentage of contributing area classified as Woody Wetlands Percentage of contributing area classified as Emergent Herbaceous Wetlands RK RK RK RK RK RK NLCD NLCD NLCD NLCD NLCD NLCD NLCD NLCD NLCD NLCD NLCD NLCD NLCD NLCD NLCD