Supplemental figure legends
Figure S1
Expression of DSG1, DSG2 and DSG3 in control tissue samples
Immunohistochemical staining of DSG1, DSG2 and DSG3 in exemplary skin, colon mucosa,
esophageal squamous epithelium and skeletal muscle tissue. 200-fold magnification. Scale bars
indicate 50 µm.
Figure S2
Patient age has no significant effect on survival of PDAC patients.
Univariate analysis (Kaplan-Meier curves and log-rank tests) of patient age as prognostic parameter in
resected PDAC patients. Crossed lines indicate censored cases.
Figure S3
DSG3 is overexpressed in PDAC tissue.
Heatmap representing the relative expression of DSG1, DSG2 and DSG3 mRNAs in PDAC and
normal pancreatic tissue. Oncomine (http://oncomine.org) was used to extract data from 5 normal and
10 PDAC samples from (Logsdon et al, 2003). Fold change (FC) was calculated as the mean of PDAC
samples divided by the mean of control samples for each gene. For statistical analysis the MannWhitney U-test was applied.
Figure S4
DSG1, DSG2 and DSG3 expression is similar in the tumor center and the tumor margin
Immunohistochemical staining of DSG1, DSG2 and DSG3 in exemplary PDAC tissue from the tumor
center and the corresponding tumor margin. 200-fold magnification. Scale bars indicate 50 µm.
Figure S5
DSG3 expression correlates with inferior survival of PDAC patients .
Univariate correlation analysis (Kaplan-Meier curves and log-rank tests) of DSG3 expression and
postoperative survival in (A) low grade tumor patients and (B) high grade tumor patients. Crossed
lines indicate censored cases.
1
Figure S7
DSG1 expression does not correlate with survival of PDAC patients.
Univariate analysis (Kaplan-Meier curve and log-rank test) in a TCGA RNA-Seq dataset of PDAC
tissue samples examining DSG1 expression levels as dichotomous variable after defining a cut-off via
ROC analysis. Crossed lines indicate censored cases.
Figure S9
DSG3 expression is a negative prognostic factor in PDAC patients.
Univariate analysis (Kaplan-Meier curve and log-rank test) by using the SurvExpress online platform
and applying microarray expression data from the GSE21501 dataset, correlating (A) DSG3, (B)
DSG1 and (C) DSG2 with patient survival.
Supplemental table legend
Table S6
Table S6 – Cox regression model, including age, stage, tumor grade, lymphatic and blood vessel
invasion, perineural invasion and expression level of DSG1, DSG2 and DSG3
Table S8
Correlation of DSG1, DSG2 and DSG3 expression and median survival times in the RNA-Seq TCGA
dataset.
2