Beatson Institute for Cancer Research. Risk Assessment. Assessed by : Margaret O'Prey Research group: BAIR Date of Assessment: 170513 Assessment Ref No: Review Due :170515 Signature : For the purpose of this risk assessment the following system of scoring will be applicable: Probability Rating: 1. Remote possibility or unlikely to occur. 2. Likely to occur in time if no preventive measures taken. 3. Likely to occur in the immediate or near future. Severity Rating: 1. May cause minor injury, illness, or property damage, no lost time. 2. May cause major injury or illness or property loss resulting in time loss. 3. May cause serious or fatal injury/illness or catastrophic loss of property. Risk Factor: =Probability Rating x Severity Rating. 1-3: Eliminate if possible: not an emergency. 4-6: Requires urgent attention, ASAP. 9: Immediate corrective action is necessary, stop working. Please complete Sections 2 – 8 as appropriate first, then complete Section 1 as a summary of all risks Section 1: Summary Description of Process Using the BAIR Timelpase Microscopes Nature of Process: Physical procedure Area in which process is to be carried out Level 1 new building Who might be affected by any significant hazard? self/neighbour/disposal staff Nature of hazard: Chemical Electrical Biological Radiation Risk Reduction Measures Can risk be minimised by System of Work yes no If yes specify means of minimising: Can risk be minimised by personal protective clothing or equipment (PPE -this should only be a last resort) yes no If yes specify what PPE will be used: gloves/ Specify if special training is required to reduce risk Microscope training 1. Sign hard copy of form and keep with group Risk Assessments 2. Email electronic version of completed form to Robert McFarlane or Bob Blackie 3. Review date should be not more than 2 years from date of risk assessment Specify any Extra First Aid/ specialist equipment needed Description of process Briefly describe the experimental procedure indicating what stage(s) present the greatest hazards (e.g. initial preparation of reagents from stock) and how the chemicals listed above are used together). Microscope hazard depends on the procedure. | Care should be taken with unfixed tissue culture cell lines not to contaminate the microscope with cell spillages. (wear gloves). | When using the fluorescent microscopes the operator should avoid looking at the UV light directly for long periods of time. If the UV/Xenon bulb explodes the room should be vacated for 30min. | Small amounts of methanol should be used for cleaning the lenses again gloves should be woren.Care should be taken not to spill the methanol around the microscope area or sit it close to the UV electrical power supplies. | Small amounts of oil should be used if required again avoid contaminating area and skin (wear gloves ). | Look out for broken glass around and on the microscope as slides are sometimes broken and fragments of glass left by previous operator Specify how waste produced will be disposed of: cinbin and Tc waste bag Specify actions in the event of a spill or accident: see BAIR staff for clean up Specify actions in the event of loss of services (e.g. electricity to fume hoods etc) Is health surveillance required? no Probability Rating after controls Severity Rating after controls Risk Factor(P x S) after controls 1. Sign hard copy of form and keep with group Risk Assessments 2. Email electronic version of completed form to Robert McFarlane or Bob Blackie 3. Review date should be not more than 2 years from date of risk assessment 2 1 2 Section 2: COSHH : Chemicals Hazardous Route of hazardous exposure Chemical Beatson Physical Amount COSHH state of purchased properties to be rating chemical: –see used MSDS sheet methanol 2 Liquid beatson store zeiss oil 1 Liquid 10x 25ml Xenon bulbs gas 10 tissue culture cells Solid flammable inhalation +ingestion + skin absorbtion liquid and vapor | poison | harmful if inhaled or absorbed through skin R38 | ingestion + skin absorption irritating to skin UV light | inhalation + ingestion Hazard from the mercury if the bulb explodes 1. Sign hard copy of form and keep with group Risk Assessments 2. Email electronic version of completed form to Robert McFarlane or Bob Blackie 3. Review date should be not more than 2 years from date of risk assessment Amount Frequency Time of Exposure to be of use exposure limits? used See MSDS Daily minutes Daily minutes Daily hours Daily hours 1. Sign hard copy of form and keep with group Risk Assessments 2. Email electronic version of completed form to Robert McFarlane or Bob Blackie 3. Review date should be not more than 2 years from date of risk assessment Section 3: COSHH - Biological agents Genetic manipulation ref. no. (if applicable) Description and source of organism/ material/ procedure Amount to be used Specify all hazards other than GMO. Risk reduction measures to be taken Section 4: Equipment Describe equipment used: Has all equipment required been checked for safe operation: yes no Are all safety interlocks in place yes no Has all equipment required been checked for safe operation: yes no Is a permit to work required? yes no Does surrounding area require protective measures? yes no Risk reduction measures: Section 5: Maintenance Process being undertaken: If yes what measures are required? Risk reduction measures: Section 6: Fire. What combustible is to be used? 1. Sign hard copy of form and keep with group Risk Assessments 2. Email electronic version of completed form to Robert McFarlane or Bob Blackie 3. Review date should be not more than 2 years from date of risk assessment What sources of ignition are around? Is a permit to work required? yes no Risk reduction measures Section 7: Physical. What is the nature of the physical hazard? Has all equipment required been checked for safe operation? Risk reduction measures: Section 8: Radiation. Radionuclide to be used: Activity (in MBq) Chemical form Frequency and duration Nature of radiation hazard Type of monitoring to be used: Risk reduction measures: 1. Sign hard copy of form and keep with group Risk Assessments 2. Email electronic version of completed form to Robert McFarlane or Bob Blackie 3. Review date should be not more than 2 years from date of risk assessment yes no