Nikon Timelapse Microscopes

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Beatson Institute for Cancer Research.
Risk Assessment.
Assessed by : Margaret
O'Prey
Research group: BAIR
Date of Assessment: 170513
Assessment Ref No:
Review Due :170515
Signature :
For the purpose of this risk assessment the following system of scoring will be applicable:
Probability Rating:
1. Remote possibility or unlikely to occur.
2. Likely to occur in time if no preventive measures taken.
3. Likely to occur in the immediate or near future.
Severity Rating:
1. May cause minor injury, illness, or property damage, no lost time.
2. May cause major injury or illness or property loss resulting in time loss.
3. May cause serious or fatal injury/illness or catastrophic loss of property.
Risk Factor:
=Probability Rating x Severity Rating.
1-3: Eliminate if possible: not an emergency.
4-6: Requires urgent attention, ASAP.
9: Immediate corrective action is necessary, stop working.
Please complete Sections 2 – 8 as appropriate first, then complete Section 1 as a
summary of all risks
Section 1: Summary
Description of Process
Using the BAIR Timelpase Microscopes
Nature of Process: Physical procedure
Area in which process is to be carried out Level 1 new building
Who might be affected by any significant hazard?
self/neighbour/disposal staff
Nature of hazard: Chemical
Electrical
Biological
Radiation
Risk Reduction Measures
Can risk be minimised by System of Work
yes
no
If yes specify means of minimising:
Can risk be minimised by personal protective clothing or equipment
(PPE -this should only be a last resort) yes
no
If yes specify what PPE will be used:
gloves/
Specify if special training is required to reduce risk
Microscope training
1. Sign hard copy of form and keep with group Risk Assessments
2. Email electronic version of completed form to Robert McFarlane or Bob Blackie
3. Review date should be not more than 2 years from date of risk assessment
Specify any Extra First Aid/ specialist equipment needed
Description of process
Briefly describe the experimental procedure indicating what stage(s) present the
greatest hazards (e.g. initial preparation of reagents from stock) and how the
chemicals listed above are used together).
Microscope hazard depends on the procedure. | Care should be taken with unfixed
tissue culture cell lines not to contaminate the microscope with cell spillages. (wear
gloves). | When using the fluorescent microscopes the operator should avoid looking
at the UV light directly for long periods of time. If the UV/Xenon bulb explodes the
room should be vacated for 30min. | Small amounts of methanol should be used for
cleaning the lenses again gloves should be woren.Care should be taken not to spill the
methanol around the microscope area or sit it close to the UV electrical power
supplies. | Small amounts of oil should be used if required again avoid contaminating
area and skin (wear gloves ). | Look out for broken glass around and on the
microscope as slides are sometimes broken and fragments of glass left by previous
operator
Specify how waste produced will be disposed of: cinbin and Tc waste bag
Specify actions in the event of a spill or accident: see BAIR staff for clean up
Specify actions in the event of loss of services (e.g. electricity to fume hoods etc)
Is health surveillance required?
no
Probability Rating after controls
Severity Rating after controls
Risk Factor(P x S) after controls
1. Sign hard copy of form and keep with group Risk Assessments
2. Email electronic version of completed form to Robert McFarlane or Bob Blackie
3. Review date should be not more than 2 years from date of risk assessment
2
1
2
Section 2:
COSHH : Chemicals
Hazardous Route of hazardous exposure
Chemical Beatson Physical Amount
COSHH state of
purchased properties
to be
rating
chemical:
–see
used
MSDS
sheet
methanol
2
Liquid
beatson
store
zeiss oil
1
Liquid
10x 25ml
Xenon
bulbs
gas
10
tissue
culture
cells
Solid
flammable inhalation +ingestion + skin absorbtion
liquid and
vapor |
poison |
harmful if
inhaled or
absorbed
through
skin
R38 |
ingestion + skin absorption
irritating
to skin
UV light | inhalation + ingestion
Hazard
from the
mercury if
the bulb
explodes
1. Sign hard copy of form and keep with group Risk Assessments
2. Email electronic version of completed form to Robert McFarlane or Bob Blackie
3. Review date should be not more than 2 years from date of risk assessment
Amount Frequency Time of Exposure
to be
of use
exposure limits?
used
See
MSDS
Daily
minutes
Daily
minutes
Daily
hours
Daily
hours
1. Sign hard copy of form and keep with group Risk Assessments
2. Email electronic version of completed form to Robert McFarlane or Bob Blackie
3. Review date should be not more than 2 years from date of risk assessment
Section 3:
COSHH -
Biological agents
Genetic manipulation ref. no. (if applicable)
Description and source of organism/ material/ procedure
Amount to be used
Specify all hazards other than GMO.
Risk reduction measures to be taken
Section 4:
Equipment
Describe equipment used:
Has all equipment required been checked for safe operation:
yes
no
Are all safety interlocks in place
yes
no
Has all equipment required been checked for safe operation:
yes
no
Is a permit to work required?
yes
no
Does surrounding area require protective measures?
yes
no
Risk reduction measures:
Section 5:
Maintenance
Process being undertaken:
If yes what measures are required?
Risk reduction measures:
Section 6:
Fire.
What combustible is to be used?
1. Sign hard copy of form and keep with group Risk Assessments
2. Email electronic version of completed form to Robert McFarlane or Bob Blackie
3. Review date should be not more than 2 years from date of risk assessment
What sources of ignition are around?
Is a permit to work required?
yes
no
Risk reduction measures
Section 7:
Physical.
What is the nature of the physical hazard?
Has all equipment required been checked for safe operation?
Risk reduction measures:
Section 8:
Radiation.
Radionuclide to be used:
Activity (in MBq)
Chemical form
Frequency and duration
Nature of radiation hazard
Type of monitoring to be used:
Risk reduction measures:
1. Sign hard copy of form and keep with group Risk Assessments
2. Email electronic version of completed form to Robert McFarlane or Bob Blackie
3. Review date should be not more than 2 years from date of risk assessment
yes
no
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