QIAquick gel extraction kit
1.
Excise the DNA fragment from the agarose gel with a clean sharp scalpel.
2.
Add 750ul Buffer QG.
3.
Incubate in the 420C water bath for approx. 10 minutes until the gel slice has completely
dissolved. To help dissolve gel, mix by vortexing the tube every 2-3 minutes during
incubation.
4.
After the gel slice has dissolved completely add 250ul isopropanol and mix.
5.
Add sample to QIAquick column and centrifuge at 13000rpm for 1 minute.
6.
Discard flow-through and place QIAquick column back in the same collection tube.
7.
Add 500ul Buffer QG to QIAquick column and centrifuge for 1 minute.
8.
Discard flow-through and place QIAquick column back in the same collection tube.
9.
Add 750ul Buffer PE (make sure ethanol has been added) and centrifuge for 1 minute.
10. Discard flow-through and place QIAquick column back in the same collection tube.
11. Centrifuge the empty column for 1 minute.
12. Place QIAquick column in a clean 1.5ml eppendorf with the lid cut off.
13. Elute the DNA by adding 15ul sterile milliQ water. Leave for 1 minute at room temperature.
Spin for 1 minute.