Introduction to Biotechnology Workshop for High School Teachers
Workshop Dates
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June 26th to June 28th
Workshop Site
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Anderson High School Austin, Texas, 8403 Mesa Drive 78705
o Laboratory used includes all equipment needed to run wet labs. This room is used to
teach Biotechnology as an articulated/dual credit course throughout the school year and
is fully equipped and in compliance with ACC Science safety regulations to accommodate
up to 20 participants for performing wet labs. The room also has a class set of netbook
computers, access to wireless printing, media station, projector and an innovation media
station for presentations.
Purpose: To host and conduct professional development workshops in biotechnology for high school
educators to:
• Learn about the tools, foundational science, and applications of biotechnology;
• Encourage student interest in relevant science, technology, and careers;
• Support the new CTE Texas Essential Knowledge and Skills for Advanced Biotechnology in
alignment with ACC Articulated Biology 1414, Introduction to Biotechnology
Workshop Participant Benefits
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Professional Development Certificate by ACC Biotechnology Program
Access to literature, curriculum and media materials from Texas Education Agency and ACC
Biotechnology High School Outreach Program
A CD or flash drive of materials presented at the workshop
Workshop Objectives
 Workshop participants should learn:
o The fundamental scientific concepts underlying biotechnology and relevant laboratory
techniques
o Effective methods for teaching these scientific concepts and laboratory techniques in
the classroom
o Effective strategies for integrating workshop materials into participants courses
o Hands-on laboratory activities that illustrate concepts (if applicable)
o Applications of the techniques and technologies related to the chosen topic (if
appropriate)
o Societal issues raised by the technologies (if applicable)
Workshop Instructional Team:
 Angela ACC Biotechnology Faculty: Lecture, lead discussion and wet labs
 Jennifer Lazare, High School Master Biotechnology Teacher, ACC Adjunct Faculty: assist
with wet labs, demonstrate activities, and provide pedagogy and practical information on how
to implement biotechnology in the classroom
Workshop Content: Based on Texas Education Agency Biotechnology Teacher Training Materials and
ACC Introduction to Biotechnology Course. Instructors will blend lecture/discussions with hands-on
activities (wet and dry labs and pedagogically useful web sites).
Topics
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DNA structure and function
Introduction to gene expression and gene regulation
Protein structure and function and their relationship to specific phenotypes/traits
Restriction enzymes, their role in recombinant DNA technology, and restriction analysis of DNA
Transformation of E. coli
Molecular biology of gene cloning
Current commercial applications of biotechnology
Societal issues related to applications of biotechnology, including one or more of the following:
genetically engineered plants, stem cell research and ramifications of the Human Genome
Project
Biotechnology careers and their education requirements.
Basic Biotechnology Wet Lab Skills:
 Proper use, maintenance and troubleshooting of biotechnology tools/equipment: micropipette,
electrophoresis apparatus, thermocycler
 Safely growing, storing and disposing of E. coli in the lab; proper handling of cultures and
 Making solutions and media needed for classroom experiments
 Preparing, handling, and staining agarose gels
 Classroom preparation for wet labs
Advanced Biotechnology Wet Lab Skills: Protein Purification, Bioinformatics, Student Capstones
Schedule of Events
Day 1
Biotech Basics
Background
Lecture/Discussions
8:30-10:00
What is
Biotechnology?
Survey of Industry
10:15-11:00
11:15-12:15
12:15-1:00
1:00-1:30
Using the Micro
pipette
Solutions and
Dilutions
Electrophoresis
1:30-2:30
2:45-4:00
How to fund your
Biotechnology
Course/Best
practices/Resources
Demo/Student
Activities Available
for practice or
sampling
Biotech Timeline
Wet Lab
Lab Materials
DNA Extraction ad
Precipitation
Genes in a Bottle
166-2300EDU
Movie Maker
Project
Current Events
Math Skills Excel
Pipetting
Setting up a
Scientific Notebook
Solutions and
Dilutions
LUNCH
Virtual
Casting an agarose
Electrophoresis Labs
gel
Mapping the Human Dye Electrophoresis
Genome
Calcium Chloride
and Magnesium
Sulfate
Day 2
Biotech Basics
Background
Lecture/Discussions
8:30-9:30
Restriction Enzymes
9:30-10:00
Medical Diagnostics
Demo/Student
Activities Available
for practice or
sampling
Paper Restriction
Gel
RFLP Webquest
10:00-10:15
10:15-10:45
Wet Lab
Lab Materials
Restriction digestion
of Lambda: set up
digest and incubate
Restriction
Digestion of Lambda
166-0002EDU
Agaorse Gels
Edvotek: Gene
Cancer, Paternity,
Cholesterol, Sickle
cell, Crime Scene
DNA
RFLP case studies:
Load and run gels
Load and run
restriction digest
Classroom
management of
materials
11:00-12:15
Stan and interpret
gels
LUNCH
pGlo transformation
(grow overnight)
12:15-1:15
1:30-2:30
Transformation
3:00-4:00
PCR
Paper PCR
Virtual PCR
Crime Scene PCR:
set up reaction-PCR
overnight
Day 3
Biotech Basics
Background
Lecture/Discussions
Wet Lab
8:30-10:00
PCR Diagnostics
Demo/Student
Activities Available
for practice or
sampling
CSI Web Adventure
10:15-11:00
11:00-12:00
Biotechnology
Curriculum and best
practices
Immunoassays
12:00-1:00
1:00-2:30
Bioinformatics/NCBI
3:00-4:00
Creating a Teacher
Workgroup PLC
pGLO™ Bacterial
Transformation Kit
166-0003EDU
Crime Scene
Investigator: PCR
Basics Kit
166-2600EDU
Lab Materials
Run and interpret
PCR
Project Share/TEA
Resources/ACC
Resources
ELISA
LUNCH
GFP Bioinformatics
pGlo analysis and
Bioinformatics
Student Capstone
Projects
ELISA Immno Kit
166-2400EDU