Read me document for data file from Vitamin D receptor gene expression and function in a South
African population.
Sheet 1: data of the larger study comprising 60 South African individuals.
Sheet 2: data for a smaller study (n = 8) in which the effect of increased vitamin D on VDR function was
assessed (Note that genotype data for these 8 individuals are not available). In sheet 2, data is
presented in a similar fashion as in sheet 1, however for each variable two additional columns is used
containing data for 5 x the normal vitamin D concentration and 10 x the normal vitamin D concentration,
respectively.
Column 1: Numbers the individuals taking part in the study from 1 – 61 (Note that individual 38 was
excluded based on ethnicity – Indian).
Column 2: Give the ethnicity of each individual. Ethnicity is classified as either White or African. African
donors belonged to any one of the 4 major ethnic groups living in South Africa. These include the Nguni,
Sotho, Shangaan-Tsonga and Venda groups.
Column 3: Gives the genotype of the FokI SNP (rs2228570) in the human Vitamin D Receptor (VDR)
gene, genotyped by means of pyrosequencing.
Column 4: Basal 25(OH)D3 status of each individual as measured with LC-MS. Data listed were corrected
with QC samples from DEQAS.
Column 5: Basal level of VDR gene expression (0h/ at the time of collection), as measured by means of
qRT-PCR. Data is ln-transformed.
Column 6: Level of VDR gene expression after 24 h culture, as determined by qRT-PCR. Data is lntransformed.
Column 7: Level of VDR gene expression after 24 h culture containing 10 nM 1,25(OH)2D3, as
determined by qRT-PCR. Data is ln-transformed.
Column 8: Basal level of VDR protein (0h/ at the time of collection), as measured by means of Flow
Cytometry.
Column 9: Level of VDR protein after 24 h culture, as measured by Flow Cytometry.
Column 10: Level of VDR protein after 24 h culture containing 10 nM 1,25(OH)2D3, as measured by Flow
Cytometry.
Column 11: Basal level of CAMP gene expression (0h/ at the time of collection), as measured by means
of qRT-PCR. Data is ln-transformed.
Column 12: Level of CAMP gene expression after 24 h culture, as determined by qRT-PCR. Data is lntransformed.
Column 13: Level of CAMP gene expression after 24 h culture containing 10 nM 1,25(OH)2D3, as
determined by qRT-PCR. Data is ln-transformed.
Column 14: Basal level of CYP24A1 gene expression (0h/ at the time of collection), as measured by
means of qRT-PCR. Data is ln-transformed
Column 15: Level of CYP24A1 gene expression after 24 h culture, as determined by qRT-PCR. Data is lntransformed.
Column 16: Level of CYP24A1 gene expression after 24 h culture containing 10 nM 1,25(OH)2D3, as
determined by qRT-PCR. Data is ln-transformed.