Research Services University of Tasmania Private Bag 01 Hobart, Tasmania 7001 Tel: (03) 6226 7283 Fax: (03) 6226 7148 University of Tasmania Animal Ethics Committee REPORT FORM animal.ethics@utas.edu.au Important Note: This is an ongoing report document to be used for the duration of the project. Reporting details for subsequent years are to be added to the form on the anniversary of initial approval of the project. Reports must be submitted one month before the anniversary date of original approval permit Details of the project Project No: Title of approved project: Responsible investigator: Original approval date of the project: (please refer to the original approval permit) Briefly re-state the aims of the project: First Year Project Report From (Date): 1. To (Date): Please provide clear information regarding the progress of the study: a. In narrative form, briefly re-state the aims of the project and the outcomes so far in relation to each aim. (To assist, an example of an appropriate answer follows) Example only Aim 1 – Has been completed and data analysed and submitted for publication. The manuscript is currently under review. Through this work we found that MND pathology is likely to be initiated and the spinal cord and spread through a proximal to distal gradient to the affected neuromuscular junctions. This work has been presented at numerous scientific conferences. Aim 2 – Live imaging protocols have been successfully established and are currently being utilised for experiments as planned. It is expected that this data will be analysed and submitted for publication in late 2014. Dr Catherine Blizzard is presenting the preliminary data from this study at the upcoming Federation of European Neuroscience Meeting to be held in Milan in July. Aim 3 – The first trial investigating the effect of EpoD on the SOD1 transgenic model is nearing completion (work performed by Jayden Clark) with the last behavioural experiments expected to be performed over the next couple of months. Due to logistics of the student performing behavioural experiments on a large number of mice at once, it was decided that the second trial looking at treatment effects after the kainic acid infusion in the YFP transgenic would be performed as a stand-alone experiment. It will likely commence late 2014 – depending on the progress Jayden is making with other aspects of his project. Animal Ethics Committee Version October 2015 Page 1 of 9 b. Supply details of publications, seminars, student completions, resulting grants and any other outcomes. 2. Have there been any issues that have interfered with the progress of the project? 3. Were there any adverse incidents or unexpected deaths? Yes/ No - If yes please attach a copy of your previously submitted Unexpected Event Report form. - If yes, please advise what changes, if any, have been made to the study to avoid the re-occurrence of such an event. 4. How many animals have been used during the period covered by this report? Species/Strain (Common name) Total number approved for project Number used during this reporting period Location1/Institution (refer to endnotes for the correct location code) 5. If animal tags have been used, please advise how many and the number of tags retrieved. 6. Any additional information can be entered below. If there are major discrepancies between the number of animals requested and the number used you must provide an explanation below. (Please Note: any discrepancies between the number of animals required and the number of animals delivered by a supplier MUST be addressed. Full details of the fate of all animals that have been oversupplied must be included in your annual report). 7. In the last 12 months, have you or any associate investigators, performed any techniques/procedures defined as a veterinary service2 under the Veterinary Service Regulation 2012? No □ Yes □ Please complete the table below Animal Ethics Committee Version October 2015 Page 2 of 9 Name of Investigator Name of Procedure/Technique Number of times performed in last 12 months 8. I wish to renew the application and accept the conditions of approval of this project. Yes/ No DECLARATION I declare that to the best of my knowledge, the information contained in this report is complete and correct. RESPONSIBLE INVESTIGATOR OR NOMINEE Name of Investigator or Nominee: Signature: ......................................................................... Date: DECLARATION FOR STUDENTS (Year 1) Declaration for students involved in a teaching project involving live animals (To allow for signatures, this form may be printed, scanned and submitted with your application) AEC approval number: Responsible investigator: Please sign to certify the following: I am familiar and will comply with all relevant Commonwealth and State or Territory legislation and the requirements of the Australian Code for the Care and Use of Animals for Scientific Purposes 8th Edition 2013. I accept that I have a responsibility to ensure that the investigations and procedures proposed in this form are conducted fully within the conditions laid down by the Code and I undertake not to use any animals or carry out any procedures which have not been approved by the Committee. I accept that the responsible investigator has ultimate responsibility for the project. Full Name Animal Ethics Committee Version October 2015 Signature Date Page 3 of 9 Second Year Project Report From (Date): To (Date): 1. Please provide information regarding the progress of the study: a. In narrative form, briefly re-state the aims of the project and the outcomes so far in relation to each aim. (To assist, an example of an appropriate answer follows) Example only Aim 1 – Has been completed and data analysed and submitted for publication. The manuscript is currently under review. Through this work we found that MND pathology is likely to be initiated and the spinal cord and spread through a proximal to distal gradient to the affected neuromuscular junctions. This work has been presented at numerous scientific conferences. Aim 2 – Live imaging protocols have been successfully established and are currently being utilised for experiments as planned. It is expected that this data will be analysed and submitted for publication in late 2014. Dr Catherine Blizzard is presenting the preliminary data from this study at the upcoming Federation of European Neuroscience Meeting to be held in Milan in July. Aim 3 – The first trial investigating the effect of EpoD on the SOD1 transgenic model is nearing completion (work performed by Jayden Clark) with the last behavioural experiments expected to be performed over the next couple of months. Due to logistics of the student performing behavioural experiments on a large number of mice at once, it was decided that the second trial looking at treatment effects after the kainic acid infusion in the YFP transgenic would be performed as a stand-alone experiment. It will likely commence late 2014 – depending on the progress Jayden is making with other aspects of his project. b. Supply details of publications, seminars, student completions, resulting grants and any other outcomes. 2. Have there been any issues that have interfered with the progress of the project? 3. Were there any adverse incidents or unexpected deaths? Yes/ No - If yes please attach a copy of your previously submitted Unexpected Event Report form. - If yes, please advise what changes, if any, have been made to the study to avoid the re-occurrence of such an event. 4. How many animals have been used during the period covered by this report? Species/Strain (Common name) Total number approved for project Animal Ethics Committee Version October 2015 Number used during this reporting period Location1/Institution (refer to endnotes for the correct location code) Page 4 of 9 5. Any additional information can be entered below. If there are major discrepancies between the number of animals requested and the number used you must provide an explanation below: (Please Note: any discrepancies between the number of animals required and the number of animals delivered by a supplier MUST be addressed. Full details of the fate of all animals that have been oversupplied must be included in your annual report). 6. In the last 12 months, have you or any associate investigators, performed any techniques/procedures defined as a veterinary service1 under the Veterinary Service Regulation 2012? No □ Please complete the table below Name of Investigator Yes □ Name of Procedure/Technique Number of times performed in last 12 months 7. I wish to renew the above application and I accept the conditions of approval of this project: Yes/ No DECLARATION I declare that to the best of my knowledge, the information contained in this report is complete and correct. RESPONSIBLE INVESTIGATOR OR NOMINEE Name of Investigator or Nominee: Signature: ......................................................................... Date: DECLARATION FOR STUDENTS (Year 2) Declaration for students involved in a teaching project involving live animals when (To allow for signatures, this form may be printed, scanned and submitted with your application) Animal Ethics Committee approval number: Responsible investigator: Animal Ethics Committee Version October 2015 Page 5 of 9 Please sign to certify the following: I am familiar and will comply with all relevant Commonwealth and State or Territory legislation and the requirements of the Australian Code for the Care and Use of Animals for Scientific Purposes 8th Edition 2013. I accept that I have a responsibility to ensure that the investigations and procedures proposed in this form are conducted fully within the conditions laid down by the Code and I undertake not to use any animals or carry out any procedures which have not been approved by the Committee. I accept that the responsible investigator has ultimate responsibility for the project. Full Name Signature Date Final Year Project Report This section must be completed when the project has been completed From (Date): To (Date): 1. Status of the Project: Has the project been completed? Has the project been abandoned? The project is continuing, and a new initial application has or will be submitted. Yes / No Yes / No Yes / No I understand that no animal experimentation may take place unless the AEC approval is current? 2. In the overall project, to what extent were the specific aims achieved? (max ½ page) 3. Were there any adverse incidents or unexpected deaths? Yes / No If yes, please provide details: 4. Provide details of how the outcomes of the research have been communicated, i.e. publications, presentations to stakeholders etc. Please append copies or abstracts where appropriate. If results are not to be published, please explain why. Animal Ethics Committee Version October 2015 Page 6 of 9 5. How many animals have been used during the period covered by this report? Species/Strain (Common name) Total number approved for project Number used during this reporting period Total number used in project Location1/Institution (refer to endnotes for the correct location code) 6. In the last 12 months, have you or any associate investigators performed any techniques/procedures defined as a veterinary service1 under the Veterinary Service Regulation 2012? No □ Yes □ Please complete the table below Name of Investigator Name of Procedure/Technique Number of times performed in last 12 months 7. Any additional information or comments can be entered below. If there were major discrepancies between the number of animals approved and the number used you must provide an explanation below: 8. Please provide any overall comments, suggestions or recommendations in respect of the use of and/or welfare of animals, based on your observations and experience from this project, below: DECLARATION I declare that to the best of my knowledge, the information contained in this report is complete and correct. RESPONSIBLE INVESTIGATOR OR NOMINEE Name of Investigator or Nominee: Signature: ......................................................................... Date: DECLARATION FOR STUDENTS (Final Year) Animal Ethics Committee Version October 2015 Page 7 of 9 Declaration for students involved in a teaching project involving live animals when (To allow for signatures, this form may be printed, scanned and submitted with your application) Animal Ethics Committee approval number: Responsible investigator: Please sign to certify the following: I am familiar and will comply with all relevant Commonwealth and State or Territory legislation and the requirements of the Australian Code for the Care and Use of Animals for Scientific Purposes 8th Edition 2013. I accept that I have a responsibility to ensure that the investigations and procedures proposed in this form are conducted fully within the conditions laid down by the Code and I undertake not to use any animals or carry out any procedures which have not been approved by the Committee. I accept that the responsible investigator has ultimate responsibility for the project. Full Name Signature Date ‘Location’ refers to the state/country in which the animals were used. Please select one number from the list below. If the same species has been used in 2 or more different locations, please select number 10 and provide a breakdown of the number of animals used in each location in the ‘Additional information’ box below the table. 1. Tasmania 2. Victoria 3. New South Wales 4. Queensland 5. Northern Territory 6. South Australia 7. Western Australia 8. Australian Capital Territory 1 Animal Ethics Committee Version October 2015 Page 8 of 9 9. Overseas 10. Species used in more than one state/country (please provide a breakdown of the number of animals used in each location in the ‘Additional Information’ box below the table). 22 2 Veterinary Surgeons Act and Regulation Extracts Veterinary services means services that form part of the practice of veterinary surgery and includes, but is not limited to including, the following services: 1. the examination of or attendance on any animal for the purposes of diagnosing the physiological or pathological condition of the animal, including testing or imaging for diagnostic purposes; 2. giving advice based on a diagnosis referred to in Item 1, including prescribing treatment, drugs, medications or medical appliances; 3. performing medical or physical treatment of animals; 4. performing surgical procedures on animals; 5. administering an anaesthetic to any animal; 6. conducting pregnancy testing of any animal; 7. carrying out, by manual operation or use of instrumental appliances, any procedure on an animal for artificial breeding purposes – For the purposes of the definition of veterinary services in Section 3(1) of the Act, the following are prescribed services and are NOT included. 1. 2. 3. 4. collection of faecal samples; collection of milk samples; collection of blood samples at the direction of a registered veterinary surgeon; administration of veterinary medicines, in accordance with the label approved under the Ag vet Code of Tasmania in relation to that medicine, by subcutaneous or intramuscular injection, oral administration (except oesophageal intubation) or application to any external body surface; 5. anaesthetising and sedating of: 6. fish of the class Osteichthyes; or 7. sharks, rays, lampreys or other cartilaginous fish of the classes Chondrichthyes and Agnatha; 8. ear tagging, ear marking or ear tattooing of any species; 9. examination for pregnancy by the external application of ultrasound scanning in any species; 10. artificial insemination provided that the semen is introduced via the vagina and cervical canal. Animal Ethics Committee Version October 2015 Page 9 of 9