OIE Reference Laboratory Reports
Activities in 2012
Name of disease (or topic) for which you
are a designated OIE Reference
Laboratory:
Classical Swine Fever
Address of laboratory
Institute of Virology
Department of Infectious Diseases
University of Veterinary Medicine Hannover,
Foundation
Buenteweg 17
30559 Hannover, Germany
Tel.:
Tel: (+49) 511-953-8841
Fax:
Fax: (+49) 511-953-8898
e-mail address:
Volker.moennig@tiho-hannover.de
Paul.becher@tiho-hannover.de
website:
http://www.tiho-hannover.de/klinikeninstitute/institute/institut-fuer-virologie-zentrumfuer-infektionsmedizin/
Name (including Title) of Head of
Laboratory (Responsible Official):
Prof. Dr. Volker Moennig (until October 2012)
Name (including Title and Position) of OIE
Reference Expert:
Prof. Dr. Volker Moennig
Date of submission to the OIE
14.01.2013
Prof. Dr. Paul Becher (from October 2012)
Instructions
This form should be used by an OIE Reference Laboratory to report activities that took place from January
through December of the past year (2012), unless otherwise stated, and must be submitted by the end of
January every year.
Only those activities that concern the disease (or topic) for which the laboratory is recognised by the OIE
should be mentioned. The questionnaire structure follows the Terms of Reference (ToRs) for OIE Reference
Laboratories, available at:
http://www.oie.int/en/our-scientific-expertise/reference-laboratories/introduction/
Each ToR (blue italicised text) has been placed as a heading covering the group of questions related to it.
Please note the red italicised text is given as guidance and should be deleted from your report and substitute
with your data. Examples are based on past Annual Reports or have been invented.
The questionnaire represents a means of gathering information on activities carried out by OIE Reference
Laboratories and making it available to OIE Member Countries and to the OIE Reference Laboratory network.
This annual report will remain available for consultation on the OIE web site:
(http://www.oie.int/en/our-scientific-expertise/reference-laboratories/annual-reports/):
Annual reports of OIE Reference Centres, 2012
1
OIE RL for « Classical Swine Fever » – « Prof. Dr. Volker Moennig » – « Germany »
ToR: To use, promote and disseminate diagnostic methods validated according to OIE
Standards
Test recommended by the OIE
Total number of test performed last year*
Indirect diagnostic tests
Nationally
Internationally
Virus neutralization test (CSFV/ BDV/BVDV)
2
148
Antibody ELISA (CSFV)
0
20
Direct diagnostic tests
Nationally
Internationally
Virus isolation (CSFV/BDV/BVDV)
33
182
Real-time RT-PCR (Panpesti/ CSFV)
8
82
Genetic typing (CSFV phylogenetic analysis)
0
24
Antigen ELISA (CSFV)
0
24
* The numbers given in the table refer to tests of diagnostic specimen only. Additional tests were carried out
in the framework of the duties of the reference laboratory: maintenance of serum and virus collection,
inter-laboratory comparison tests, standardization of reference material and different related research
projects.
ToR: To develop reference material in accordance with OIE requirements, and implement
and promote the application of OIE Standards.
To store and distribute to national laboratories biological reference products and any
other reagents used in the diagnosis and control of the designated pathogens or
disease.
2.
Did your laboratory produce or store imported standard reference reagents officially recognised by
the OIE or other international bodies?
Yes
3.
No
Did your laboratory supply standard reference reagents to OIE Member Countries?
Yes
Type of reagent
available
Related
diagnostic
test
Cloned cell culture
PK 15 (flasks with
replicating
monolayers)
Virus isolation
Virus
Neutralization
assay
CSFV, BDV, BVDV
strains and
isolates
2
Virus
Neutralization
assay
No
Produced/
stored
stored
Produced
Amount
supplied
nationally
(ml, mg)
Amount supplied
internationally
(ml, mg)
Name of recipient OIE
Member Countries
and of institutions
1 x 25 cm3 flask
Spain
Central Veterinary
Laboratory
Algete/ Madrid
1 x 25 cm3 flask
Montenegro
Diagnostic Veterinary
Laboratory
Podgorica
8 x 1 ml
Belgium
National Reference
Laboratory
Ukkel/ Brussels
2 x 1 ml
Montenegro
Diagnostic Veterinary
Laboratory
Podgorica
Annual reports of OIE Reference Centres, 2012
OIE RL for « CSF» – « Prof. Dr. Volker Moennig » – « Germany »
Type of reagent
available
Related
diagnostic
test
Produced/
stored
Amount
supplied
nationally
(ml, mg)
Amount supplied
internationally
(ml, mg)
Name of recipient OIE
Member Countries
and of institutions
2 x 1 ml
Spain
CRESA Institute
Barcelona
4 x 0,5 ml
Complete serum
panel for ELISA
validation (1
panel with 31
different sera)
Ab ELISA
Complete serum
panel for ELISA
validation (1
panel with 31
different sera)
Ab ELISA
PCR reference
serum panel
Real-time RTPCR
Monoclonal
antibodies
directed against
CSFV, BVDV or
BDV
Virus
Neutralization
Assay
Virus Isolation
Produced
Produced
Produced
Germany
National Reference
Laboratory
Greifswald- Island of
Riems
30 x 3 ml;
3 x 1 ml
Korea
Bionote
Gyeonoggi-do
30 x 3 ml;
3 x 1 ml
Greece
Veterinary Institute of
Infectious and Parasitic
Disease
Paraskevi, Athens
30 x 3 ml;
3 x 1 ml
United Kingdom
Laboratory Services
Department, Weybridge/
Addlestone
60 x 3 ml;
6 x 1 ml
Poland, National
Veterinary Research
Institute, Pulawy
4x 2ml;
1 x 10 ml
Latvia
National Reference
Laboratory
Riga
2 x 10 ml
Hungary
Veterinary Diagnostic
Directorate, Budapest
4 x 10 ml
Poland, National
Veterinary Research
Institute, Pulawy
3 x 10 ml
Switzerland
Institute of Virology
Bern
2 x 10 ml
Lithuania
National Institute of Food
and Veterinary Risk
Assessment
Vilnius
2 x 10 ml
Finland
Finnish Food Safety
Authority EVIRA
Helsinki
Produced
Annual reports of OIE Reference Laboratories, 2012
3
OIE RL for « Classical Swine Fever » – « Prof. Dr. Volker Moennig » – « Germany »
Type of reagent
available
Related
diagnostic
test
Monoclonal
antibodies
directed against
CSFV, BVDV or
BDV
Virus
Neutralization
Assay
Virus Isolation
Reference sera
from
experimentally
infected animals
Virus
Neutralization
assay
ELISA
Produced/
stored
Amount
supplied
nationally
(ml, mg)
Produced
Amount supplied
internationally
(ml, mg)
Name of recipient OIE
Member Countries
and of institutions
5 x 10 ml
Mexico
Comisión México-Estados
Unidos para la Prevención
de la Fiebra Aftosa y otras
Enfermedades Exóticas de
los Animales
Del. Cuajimalpa
6 x 10 ml
Latvia
Institute of Food Safety ,
Animal Health and
Environment “BIOR”
Riga
2x 10 ml
Croatia
Croatian Veterinary
Institute
Zagreb
3 x 10 ml
Slovenia
National Veterinary
Institute
Ljubljana
10 x 1 ml
Belgium
CODA-CERVA-VAR
Virology
Ukkel/ Brussels
66 x 1 ml
Sweden
SVA
Uppsala
2 x 1,5 ml
Finland
Finnish Food Safety
Authority EVIRA
Helsinki
Produced
Germany
National Reference
Laboratory
Greifswald- Island of
Riems
2 x 1,5 ml
6 x 3 ml
Malaysia
VET Food Agro
Diagnostics
Petaling Jaya, Selangor
3 x 1 ml
Croatia
Croatian Veterinary
Institute
Zagreb
4.
Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference
reagents?
Yes
No
5.
Did your laboratory produce vaccines?
Yes
4
No
Annual reports of OIE Reference Centres, 2012
OIE RL for « CSF» – « Prof. Dr. Volker Moennig » – « Germany »
6.
Did your laboratory supply vaccines to OIE Member Countries?
Yes
No
ToR: To develop, standardise and validate, according to OIE Standards, new procedures for
diagnosis and control of the designated pathogens or diseases
7.
Did your laboratory develop new diagnostic methods validated according to OIE Standards for the
designated pathogen or disease?
Yes
8.
No
Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen
or disease?
Yes
No
Name of the new test
or diagnostic method
or vaccine developed
Phylogenetic analysis of
CSFV based on full-length
E2 encoding sequences
Description and References (Publication, website, etc.)
The short sequence lengths of the 5´NTR and the E2 gene fragments represent a limiting
factor for differentiation of closely related isolates and also for confidence levels of
proposed CSFV groups and subgroups. Analysis of full-length E2 encoding sequences
proved to be more suitable for reliable and statistically significant phylogeny providing a
solid basis for molecular epidemiology of CSF.
Postel A., Schmeiser S., Bernau J., Meindl-Boehmer A., Pridotkas, G., Dirbakova, Z., Mojzis,
M., and Becher P. (2012). Improved strategy for phylogenetic analysis of Classical swine
fever virus based on full-length E2 encoding Sequences. Veterinary Research 43:50;
http://www.veterinaryresearch.org/content/43/1/50.
ToR: To provide diagnostic testing facilities, and, where appropriate, scientific and
technical advice on disease control measures to OIE Member Countries
9.
Did your laboratory carry out diagnostic testing for other OIE Member Countries?
Yes
No
Name of OIE Member Country seeking
assistance
Date
(dd/mm)
No. samples received for
provision of diagnostic
support (i.e. from
surveillance campaign)
Agricultural University Tirana, Albania
28/09
26
Regional Animal Health Office, Ho Chi Minh
City, Vietnam
9/11
10
Institute of Food Safety, Animal Health and
Environment (BIOR), Riga, Latvia
16/11
18
Institute of Food Safety, Animal Health and
Environment (BIOR), Riga, Latvia
28/11
28
10.
No. samples received
for provision of
confirmatory
diagnoses
Did your laboratory provide expert advice in technical consultancies on the request of an OIE
Member Country?
Yes
No
Name of the OIE Member Country
receiving a technical consultancy
Purpose
How the advice was
provided
National Institute of Food and Veterinary
Risk Assessment, Vilnius, Lithuania
Establishing peroxidase staining for Virus
neutralization assay
In-house training and
remote assistance
Annual reports of OIE Reference Laboratories, 2012
5
OIE RL for « Classical Swine Fever » – « Prof. Dr. Volker Moennig » – « Germany »
Name of the OIE Member Country
receiving a technical consultancy
Purpose
How the advice was
provided
Central Agricultural Office. Veterinary
Diagnostic Directorate, Budapest,
Hungary
Technical advice on how to reduce toxic
components that might lead to unspecific
fluorescence in wild boar sera
Remote assistance
Department of Animal Health Ministry of
Agriculture, Brazil
Case definition for officially controlled diseases
according to clinical, epidemiological and
laboratory criteria
Remote assistance
Veterinary Institute of Infectious and
Parasitic Disease, Paraskevi-Athens,
Greece
Establishing new techniques in molecular biology
diagnostic methods in the NRL in Greece
In-house training and
remote assistance
ARC-OVI-Transboundary Animal Diseases
Programme Onderstepoort, South Africa
Information and technical advice on CSF
diagnostic real-time RT-PCR and preparation of
sample material
Remote assistance
National Veterinary Research institute,
Pulawy, Poland
Information on and practical training in the
performance of classical laboratory techniques
(Virus isolation, Virus neutralization test)
In-house training
Institute of Food Safety, Animal Health
and Environment (BIOR), Riga, Latvia
Improvement of diagnostic methods for
detection of CSFV, i.e. molecular biology.
In loco assistance
ToR: To carry out and/or coordinate scientific and technical studies in collaboration with
other laboratories, centres or organisations
11.
Did your laboratory participate in international scientific studies in collaboration with OIE Member
Countries other than the own?
Yes
Title of
the study
“CSFV_
go DIVA”
6
Duration
4 years
Purpose of the
study
Improve tools and
strategies for the
prevention and
control of
classical swine
fever
No
Partners (Institutions)
Veterinary and Agrochemical Research Centre Belgium
National Veterinary Institute, Technical University of
Denmark
French Food Safety Agency, France
Central Agricultural Office, Directorate of Veterinary
Medicinal Products, Hungary
Central Veterinary Institute, Netherlands
Fort Dodge Animal Health, Spain
Friedrich-Loeffler-Institute, Federal Research Institute
for Animal Health, Germany
Institute of Animal Health Umbria e Marche, Italy
Institute for Virology and Immunoprophylaxis,
Switzerland
The National Game and Wildlife Agency, France
Bioengineering Research Centre for Animal Disease
Prevention and Control, China
State Veterinary Institute, Sweden
University Complutense of Madrid, Spain
Helmholtz Centre for Environmental Research,
Germany
Spectos GmbH, Germany
Institute of Animal Health of Abruzzo and Molise, Italy
OIE
Member
Countries
involved
other than
your
country
Belgium
Denmark
France
Hungary
Netherlands
Spain
Italy
Switzerland
China
Sweden
Annual reports of OIE Reference Centres, 2012
OIE RL for « CSF» – « Prof. Dr. Volker Moennig » – « Germany »
Title of
the study
Duration
Purpose of the
study
Partners (Institutions)
OIE
Member
Countries
involved
other than
your
country
European
Research
Group
(ERG,
entitled
EPIZONE)
5 years
Strengthening the
cooperation
between National
and International
Reference
laboratories in the
field of epizootic
diseases.
Central Veterinary Institute (CVI), Lelystad, Netherlands
Friedrich-Loeffler-Institute (FLI), Greifswald – Island of
Riems, Germany
Institute for Animal Health (IAH), Newbury, UK
Animal Health and Veterinary Laboratories Agency
(AHVLA), Addlestone, Surrey, United Kingdom
DTU Veterinæinstituttet (DTU Vet), Copenhagen,
Denmark
Statens Veterinarmedicinska Anstalt (SVA), Uppsala,
Sweden
Centre de coopération Internationale en Recherche
Agronomique pour le Développement (CIRAD),
Monpellier, France
Center of Animal Health, National Institute for
Agriculture and Food Research and Technology (CISAINIA), Valdeolmos, Spain
Istituto Zooprofilattico Sperimentale delle Venezie,
(IZSVe), Legnaro, Italy
Lanzhou Veterinary Research Institute (LVRI), China
National Veterinary Research Institute (NVRI) Pulawy,
Poland
FMD Institute Ankara (SAP), Ankara, Turkey
VAR-CODA-CERVA (VAR-CODA), Ukkel, Belgium
Istituto Zooprofilattico Sperimentale della Lombardia e
dell´Emilia Romagna (IZS-Br), Brescia, Italy
Harbin Veterinary Research Institute (CAAS), China
The
Netherlands
Germany
United
Kingdom
Denmark
Sweden
France
Spain
Italy
China
Poland
Turkey
Belgium
OIE
Laboratory
Twinning
Project
3 years
Updating and
Revision of
Methods for
diagnosis of CSF
Evaluate
sequences of
Cuban local CSFV
isolates and
determination of
genotype, for
completing
picture of
Caribbean isolates
Development,
Evaluation and
Implementation
of a novel
isothermal nucleic
acid detection
technique for
CSFV
Evaluation of new
pen-side test for
detection of CSF
specific antibodies
Centro Nacional de Sandidad Agropecuaria, La Habana
(CENSA), Cuba
Cuba
Annual reports of OIE Reference Laboratories, 2012
7
OIE RL for « Classical Swine Fever » – « Prof. Dr. Volker Moennig » – « Germany »
Title of
the study
Duration
Purpose of the
study
Partners (Institutions)
CRTI 09403TA
2 years
Development of
ready-to-use
laboratory and
field deployable
technology for
rapid
identification and
genetic typing of
multiple HC
agents of swine
CIFA- National Center for Animal Disease, Winnipeg/
Lethbridge, Canada
Nexogen, Inc., USA
Institute of Animal Health, United Kingdom
DssFADDL-NVSL,
APHISUSDA, Plum Island, USA
DISCONTO
OLS
4 years
Providing a
mechanism for
focusing and
prioritising
research that
ultimately
delivers new and
improved
vaccines,
pharmaceuticals
and diagnostic
tests
http://www.discontools.eu/
OIE
Member
Countries
involved
other than
your
country
Canada
USA
United
Kingdom
ToR: To collect, process, analyse, publish and disseminate epizootiological data relevant to
the designated pathogens or diseases
12.
Did your Laboratory collect epizootiological data relevant to international disease control?
Yes
13.
No
Did your laboratory disseminate epizootiological data that had been processed and analysed?
Yes
14.
No
What method of dissemination of information is most often used by your laboratory?
(Indicate in the appropriate box the number by category)
a)
Articles published in peer-reviewed journals: ................. 09
b)
International conferences: .............................................. 08
c)
National conferences: ...................................................... 09
d)
Other: ............................................................................... internet:1 books: 1
ToR: To provide scientific and technical training for personnel from OIE Member Countries
To recommend the prescribed and alternative tests or vaccines as OIE Standards
15.
Did your laboratory provide scientific and technical training to laboratory personnel from other OIE
Member Countries?
Yes
8
No
a)
Technical visits: ................................................................ 4
b)
Seminars: ......................................................................... 0
Annual reports of OIE Reference Centres, 2012
OIE RL for « CSF» – « Prof. Dr. Volker Moennig » – « Germany »
c)
Hands-on training courses: .............................................. 3
d)
Internships (>1 month): ................................................... 0
Type of technical training
provided (a, b, c or d)
Country of origin of the expert(s)
provided with training
No. participants from the
corresponding country
a
Greece
1
c
Poland
2
a
Cuba
3
c
Lithuania
1
ToR: To maintain a system of quality assurance, biosafety and biosecurity relevant for the
pathogen and the disease concerned
16.
Does your laboratory have a Quality Management System certified according to an International
Standard?
Yes
No
Quality management system adopted
ISO 17025
17.
Is your laboratory accredited by an international accreditation body?
Yes
No
Test for which your laboratory is accredited
Accreditation body*
Isolation, propagation and quantification of CSFV in cell culture
DAKKS (ILAC-MRA)
Detection of CSFV antigen by ELISA
DAKKS (ILAC-MRA)
Detection of antibodies directed against CSFV by ELISA
DAKKS (ILAC-MRA)
Detection of antibodies directed against CSFV by neutralization assay
DAKKS (ILAC-MRA)
Detection of CSFV genome using RT-PCR and subsequent preparation for genotyping
DAKKS (ILAC-MRA)
Detection of CSFV genome and of genome of other pestiviruses using real-time RT-PCR
DAKKS (ILAC-MRA)
Detection of CSFV genome using real-time RT-PCR with TaqMan probe
DAKKS (ILAC-MRA)
Detection of Pestivirus genome with RT-PCR
DAKKS (ILAC-MRA)
Detection of antibodies directed against Border Disease Virus (BDV) by neutralization
assay
DAKKS (ILAC-MRA)
Detection of antibodies directed against Bovine Viral Diarrhoea Virus (BVDV) by
neutralization assay
DAKKS (ILAC-MRA)
Detection of antibodies directed against Bovine Viral Diarrhoea Virus (BVDV) by ELISA
DAKKS (ILAC-MRA)
Detection of Bovine Viral Diarrhoea Virus (BVDV) antigen by ELISA
DAKKS (ILAC-MRA)
Virus isolation, propagation and quantification of Bovine Viral Diarrhoea Virus (BVDV)
DAKKS (ILAC-MRA)
*The DAKKS (German accreditation body) accreditation falls within the scope of the multilateral agreements (MLA/MRA)
of the European co-operation for Accreditation (EA) and the international accreditation organisations IAF and ILAC.
18. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease
concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 or
Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1)
Yes
Annual reports of OIE Reference Laboratories, 2012
No
9
OIE RL for « Classical Swine Fever » – « Prof. Dr. Volker Moennig » – « Germany »
ToR: To organise and participate in scientific meetings on behalf of the OIE
19.
Did your laboratory organise scientific meetings on behalf of the OIE?
Yes
No
National/
International
Title of event
Co-organiser
Date
(mm/yy)
Location
No.
Participants
International
Workshop on Laboratory
Diagnosis of African and
Classical Swine Fever
CISA-INIA,
Madrid, Spain,
DG-SANCO
05/12
Hannover,
Germany
93
National
Expert Workshop Swine
Fever:
Clinical signs, epidemiology
and disease control
LAVES, Germany;
FLI, National RL
Germany;
AKNZ, Germany
11/12
Hannover,
Germany
21
20.
Did your laboratory participate in scientific meetings on behalf of the OIE?
Yes
Title of event
No
Date
(mm/yy)
Location
Role (speaker, presenting
poster, short
communications)
Title of the work
presented
Meeting of the Task
Force (TF) for
monitoring Animal
Disease eradication in
the member states
03/12
Brussels,
Belgium
Speaker
(V. Moennig)
Summary of the conclusions
and recommendation of the
fourth meeting held by the
sub-group on Classical Swine
fever (CSF)
22nd Annual Meeting
of the Society for
Virology
03/12
Essen, Germany
Speaker
(S. Schmeiser)
Morphogenesis of
Pestiviruses
Poster
(A. Postel)
Classical Swine Fever – A
New Strategy for Molecular
Epidemiology
Poster
(P. Becher)
Evolution of chimeric
pestiviruses by
nonhomologous RNA
recombination between
different virus species
Poster
(D. Meyer)
Crucial role of two amino
acids on the antibody
binding of the Classical
swine fever virus
glycoprotein Erns
Poster
(S. Austermann-Busch)
3´Terminal Phoshate and
5´Terminal Hydroxyl Groups
of RNA Molecules Enhance
RNA Recombination During
Viral Infection
Workshop Laboratory
Diagnosis of African
Swine Fever and
Classical Swine Fever
05-06/12
Hannover
Germany
Speaker
(A. Postel)
Imroved Strategy for
Phylogenetic Analysis of
Classical Swine Fever Virus
based on full-length E2
encoding Sequences
Workshop Laboratory
Diagnosis of African
Swine Fever and
Classical Swine Fever
05-06/12
Hannover
Germany
Speaker
(S. Schmeiser)
Is isolated RNA of CSFV
infectious for pigs?
10
Annual reports of OIE Reference Centres, 2012
OIE RL for « CSF» – « Prof. Dr. Volker Moennig » – « Germany »
Title of event
Date
(mm/yy)
Location
Role (speaker, presenting
poster, short
communications)
Title of the work
presented
Scientific conference
“Prevention of
Classical Swine Fever
in the border region
Croatia - Serbia (“Stop
CSF”)
06/12
Novi Sad, Serbia
Speaker
(S. Schmeiser)
Molecular epidemiology of
classical swine fever virus
isolates from South-eastern
Europe
CSFV-goDIVA Meeting
06/12
Visegrad,
Hungary
Speaker
(K. Pannhorst)
Serum sample panel for the
evaluation of selected ELISA
systems within an interlaboratory comparison test
IX International
Congress of Veterinary
Virology
09/12
Madrid, Spain
Poster
(P. Becher)
Molecular Characterization
of Border Disease Virus
Strain Aveyron ESVV
Introduced Intelligence
project of State
Administration of
Foreign Expert Affairs
China (SAFEA)
09/12
Beijing, China
Speaker
(P. Becher)
Diagnosis and Molecular
Epidemiology of Classical
Swine Fever
International
Workshop of
Prevention and Control
Strategies of Classical
Swine Fever 4th China
Congress of Veterinary
Medicine
09/12
Beijing, China
Speaker
(P. Becher)
Variability of Classical Swine
Fever Virus and related
viruses: Implication for
Diagnosis and Control of CSF
World Veterinary
Association Workshop
“Vaccines and
Diagnostics for
Transboundary Animal
Diseases”
09/12
Ames, USA
Speaker
(V. Moennig)
Classical Swine Fever
IPA Multibeneficiary
Project “Support for
the control/
eradication of animal
diseases”, second
regional Workshop
10/12
Belgrade,
Republic of
Serbia
Invited consultant experts in
CSF diagnosis and
epidemiology
(P. Becher, A. Postel)
Invited consultant experts in
CSF diagnosis and
epidemiology
DISCONTOOLS
Conference “Fighting
animal diseases
identifying research
priorities”
11/12
Brussels,
Belgium
Speaker
(V. Moennig)
Epizootic – Classical Swine
Fever
Expert Workshop
Classical Swine Fever:
Clinical Signs,
epidemiology and
disease control
11/12
Hannover,
Germany
Speaker
(P. Becher)
Molecular Epidemiology of
Classical Swine Fever
Speaker
(S. Austermann-Busch)
Handling and Shipment of
CSF suspicious sample
material
CSFV_goDIVA Meeting
11/12
Speaker
(K. Pannhorst)
Preliminary results of the
evaluation of selected ELISA
systems within an interlaboratory comparison test
Lelystad, The
Netherlands
Annual reports of OIE Reference Laboratories, 2012
11
OIE RL for « Classical Swine Fever » – « Prof. Dr. Volker Moennig » – « Germany »
ToR: To establish and maintain a network with other OIE Reference Laboratories
designated for the same pathogen or disease and organise regular inter-laboratory
proficiency testing to ensure comparability of results
21.
Did your laboratory exchange information with other OIE Reference Laboratories designated for the
same pathogen or disease?
Yes
22.
No
Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated
for the same pathogen or disease by organising or participating in proficiency tests?
Yes
No
Purpose of the proficiency tests:
(validation of a diagnostic protocol:
specify the test; quality control of
vaccines: specify the vaccine type, etc.)
Role of your Reference
Laboratory (organiser/
participant)
Validation:
Real-time RT-PCR
Conventional RT-PCR
Virus Neutralization assay
Virus Isolation
Antibody ELISA
Antigen ELISA
Organiser
23.
No.
participa
nts
Participating OIE Ref.
Labs/ organising OIE
Ref. Lab.
56
OIE RL for CSF, Poland
OIE RL for CSF, United
Kingdom
OIE RL for CSF, Canada
Did your laboratory collaborate with other OIE Reference Laboratories for the same disease
on scientific research projects for the diagnosis or control of the pathogen of interest?
Yes
No
Name(s) of relevant OIE
Reference Laboratories
Title of the project or contact
Scope
European Research Group (ERG,
entitled EPIZONE)
Strengthening the cooperation between National
and International Reference laboratories in the
field of Epizootic diseases
OIE RL for CSF, Poland and OIE
RL for CSF, UK
ToR: To organise inter-laboratory proficiency testing with laboratories other than OIE
Reference Laboratories for the same pathogens and diseases to ensure equivalence of
results.
24.
Did your laboratory organise or participate in inter-laboratory proficiency tests with
laboratories other than OIE Reference Laboratories for the same disease?
Yes
Purpose for inter-laboratory test comparisons1
1
12
No
No. participating
laboratories
Participating OIE Member
Countries
See Interlaboratory test comparisons in: Laboratory Proficiency Testing at:
www.oie.int/en/our-scientific-expertise/reference-laboratories/proficiency-testing see point 1.3
Annual reports of OIE Reference Centres, 2012
OIE RL for « CSF» – « Prof. Dr. Volker Moennig » – « Germany »
Purpose for inter-laboratory test comparisons1
Reference Laboratory as Organiser:
Laboratory capability to conduct specific diagnostic
tests:
Real-time RT-PCR (CSF specific and Panpesti)
Conventional RT-PCR
Virus Neutralization assay (CSFV; BVDV; BDV)
Virus Isolation
Antibody ELISA
Antigen ELISA
No. participating
laboratories
Participating OIE Member
Countries
56
Austria; Belgium; BIH; Brazil; Bulgaria;
Chile; Croatia; Cuba; Cyprus; Czech
Republic; Denmark; Dominican
Republic; Estonia; Finland; France;
Germany; Greece; Haiti; Hungary;
Ireland; Italy; Jamaica; Latvia;
Lithuania; Luxembourg; Mexico;
Montenegro; Norway; Portugal; PR
China; FYROM; Romania; Russia;
Serbia; Slovakia; Slovenia; South Africa;
Spain; Sweden; Switzerland; The
Netherlands; Trinidad and Tobago;
Ukraine; United Kingdom; USA
Reference Laboratory as Participant
Laboratory capability to conduct CSF serological
diagnostics
Organiser: National Reference Laboratory for CSF,
Germany
Reference Laboratory as Participant
Laboratory capability to conduct CSF serological
diagnostics
Organiser: National Reference Laboratory for CSF,
France
ToR: To place expert consultants at the disposal of the OIE
25.
Did your laboratory place expert consultants at the disposal of the OIE?
Yes
Kind of consultancy
No
Location
Provision of comments on review of OIE
Standards
Subject (facultative )
Amendment to Chapter 2.8.3.
classical swine fever
(hog cholera)
Attendance at conference
Fleesensee, Germany
25th Conference of the OIE Regional
Commission for Europe”
Chair of the CSF Taskforce of DG Sanco
(V. Moennig)
Brussels, Belgium
Summary of the conclusions and
recommendation of the fourth meeting
held by the sub-group on
Classical Swine fever (CSF)
Annex : List of Publications
Scientific publications in peer-reviewed journals:
Austermann-Busch, S., and Becher, P. (2012). RNA structural elements determine frequency and sites of
nonhomologous recombination in an animal plus-strand RNA virus. Journal of Virology 86, 7393–7402.
Becher, P., Schmeiser, S., Oguzoglu, T.C., and Postel, A. (2012). Complete genome sequence of a novel
pestivirus from sheep. Journal of Virology 86, 11412.
Blome, S., Meindl-Böhmer, A., Nowak, G., and Moennig, V. (2012). Disseminated intravascular coagulation
Annual reports of OIE Reference Laboratories, 2012
13
OIE RL for « Classical Swine Fever » – « Prof. Dr. Volker Moennig » – « Germany »
does not play a major role in the pathogenesis of classical swine fever. Veterinary Microbiology. [Epub ahead
of print]. PMID: 23107658
Kautto, A.H., Alenius, S., Mossing, T., Becher, P., Belák, S., and Larska, M. (2012). Pestivirus and
alphaherpesvirus infections in Swedish reindeer (Rangifer tarandus tarandus L.). Veterinary Microbiology.
156, 64–71.
Lattwein, E., Klemens, O., Schwindt, S., Becher, P., and Tautz, N. (2012). Pestivirus virion morphogenesis in
the absence of uncleaved nonstructural protein 2-3. Journal of Virology 86, 427–437.
Meyer, D., Aebischer, A., Müller, M., Grummer, B., Greiser-Wilke, I., Moennig, V., and Hofmann, M.A.
(2012). New insights into the antigenic structure of the glycoprotein E(rns) of classical swine fever virus by
epitope mapping. Virology 433, 45–54.
Postel, A., Jha, V.C., Schmeiser, S., and Becher, P. (2012). First molecular identification and characterization
of classical swine fever virus isolates from Nepal. Archives of Virology Sep 14. [Epub ahead of print]. PMID:
22975985.
Postel, A., Schmeiser, S., Bernau, J., Meindl-Boehmer, A., Pridotkas, G., Dirbakova, Z., Mojzis, M., and
Becher, P. (2012). Improved strategy for phylogenetic analysis of classical swine fever virus based on fulllength E2 encoding sequences. Veterinary Research. 43, 50.
Postel, A., Schmeiser, S., Perera, C.L., Rodríguez, L.J.P., Frias-Lepoureau, M.T., and Becher, P. (2012).
Classical swine fever virus isolates from Cuba form a new subgenotype 1.4. Veterinary Microbiology [Epub
ahead of print]. PMID: 22902191
Internet:
http://public.csf-wildboar.eu/Default.aspx
Book chapters:
Simmonds, P., Becher, P., Collett, M.S., Gould, E.A., Heinz, F.X., Meyers, G., Monath, T., Pletnev, A., Rice,
C.M., Stiasny, K., et al. (2012). Family Flaviviridae. In Virus Taxonomy. Ninth Report of the International
Committee on Taxonomy of Viruses. Edited by A.M.Q. King et al. Academic Press, San Diego, USA.
Presentations at national and international conferences and meetings:
Austermann-Busch S. and Becher P. (2012). 3´ Terminal Phosphate and 5´ Terminal Hydroxyl Groups of
RNA Molecules Enhance RNA Recombination During Viral Infection. 22nd Annual Meeting of the Society for
Virology, Essen, Germany, 14th – 17th of March 2012.
Austermann-Busch S. (2012). Handling and Shipment of CSF suspicious sample material. Expert-Workshop
Classical Swine Fever: Clinical Signs, epidemiology and disease control, Hannover, Germany, 26th –30th of
November, 2012.
Becher P., Gallei A. 2012. Evolution of chimeric pestiviruses by nonhomologous RNA recombination
between different virus species. 22nd Annual Meeting of the Society for Virology, Essen, Germany, 14th – 17th
of March 2012.
Becher P. (2012). Variability of Classical Swine Fever Virus and related viruses: Implications for Diagnosis
and Control of CSF. International Workshop of Prevention and Control Strategies of Classical Swine Fever, 4th
China Congress of Veterinary Medicine, Beijing, China 3rd-4th of September, 2012.
Becher P. (2012). Molecular Epidemiology of Classical Swine Fever. Expert-Workshop Classical Swine
Fever: Clinical Signs, epidemiology and disease control, Hannover, Germany, 26th –30th of November, 2012.
Meyer D., Moennig V. and Greiser-Wilke I. (2012). Crucial role of two amino acids on the antibody binding
14
Annual reports of OIE Reference Centres, 2012
OIE RL for « CSF» – « Prof. Dr. Volker Moennig » – « Germany »
of Classical swine fever virus glycoprotein Erns. 22nd Annual Meeting of the Society for Virology, Essen,
Germany, 14th – 17th of March 2012.
Moennig V. (2012) Summary of the conclusions and recommendation of the fourth meeting held by the subgroup on Classical Swine fever (CSF). Meeting of the Task Force for monitoring Animal Disease eradication
in the member states, Brussels, Belgium, 1st of March, 2012.
Moennig V. (2012). Classical Swine Fever. Workshop “Vaccines and Diagnostics for Transboundary Animal
Diseases”, Iowa State University, Ames, IA, USA, 17 th-19th of September 2012.
Moennig V. and Becher P. (2012). Classical Swine Fever –Scoring Exercise. Disontools Conference
“Fighting animal diseases identifying research priorities”, Brussels, Belgium, November 15th, 2012.
Pannhorst K., Meyer D., Meindl-Boehmer A., Schmeiser S., Staubach C. and Blome S. (2012). Serum
sample panel for the evaluation of selected ELISA systems within an inter-laboratory comparison test.
CSFV_goDIVA Meeting, Visegrad, Hungary, 6th- 7th of June, 2012.
Pannhorst K., Meyer D., Staubach C., Fröhlich A. and Sonnenburg J. (2012). Preliminary results of the
evaluation of selected ELISA systems within an inter-laboratory comparsion test. CSFV_goDIVA Meeting,
Lelystad, The Netherlands, 28th- 29th of November, 2012.
Postel A., Schmeiser S., Bernau J., Meindl-Boehmer A., Pridotkas G., Dirbakova Z., Mojzis M. and Becher
P. (2012). Classical Swine Fever – A New Strategy for Molecular Epidemiology. 22 nd Annual Meeting of the
Society for Virology, Essen, Germany, 14th – 17th of March 2012.
Postel A., Schmeiser S., Bernau J., Meindl-Boehmer A., and Becher P. (2012). Improved Strategy for
Phylogenetic Analysis of Classical Swine Fever Virus based on full-length E2 encoding Sequences. Workshop
Laboratory Diagnosis of African Swine Fever and Classical Swine Fever, Hannover, Germany, May 30th –
June 1st, 2012.
Schmeiser S. (2012). Morphogenesis of Pestiviruses. 22nd Annual Meeting of the Society for Virology, Essen,
Germany, 14th – 17th of March 2012.
Schmeiser S. (2012). Is isolated RNA of CSFV infectious for pigs? Workshop Laboratory Diagnosis of
African Swine Fever and Classical Swine Fever, Hannover, Germany, May 30th – June 1st, 2012.
Schmeiser S. (2012). Molecular epidemiology of classical swine fever virus isolates from South-eastern
Europe. Scientific conference “Prevention of Classical Swine Fever in the border region Croatia - Serbia
(“Stop CSF”), Novi Sad, Republic of Serbia, 7 th- 8th of June, 2012.
Vilcek S., Leskova V., Postel A., and Becher P. (2012). Molecular characterization of Border Disease Virus
strain AVEYRON ESVV. IX International Congress of Veterinary Virology, Madrid, Spain 4th – 7th September
2012.
Annual reports of OIE Reference Laboratories, 2012
15