University of Oslo
Procedure with risk assessment:
NAME OF PROCEDURE
Written by :
IBV; Cathrine E. Fagernes
1.
Saks- og dokumentnr. i ePhorte:
Approved by:
IBV; Anders Moen
S-SOP-xxx-shortname
Version:
002
Date:
05.08.2015
Page:
1 of 4
INTRODUCTION/PURPOSE
This is a procedure including a detailed risk assessment for making 6 % Perchloric acid. 6 % Perchloric
acid is used for EtOH concentration determination.
2.
RESPONSIBILITIES AND SAFETY
See the general UiO procedure Risk management policy in laboratories for an overview of
responsibilities at UiO.
General laboratory safety applies. For more information see IBV´s HSE webpages:
http://www.mn.uio.no/ibv/english/about/hss/. Key procedures for working in the laboratories are
listed in HSE in the laboratories: http://www.mn.uio.no/ibv/english/about/hss/laboratorier/
3.
NECESSARY SAFETY EQUIPMENT
When making this solution:
 Wear gloves and lab coat
 Use fume hood.
4.
EQUIPMENT, MATERIALS AND SOLUTIONS
Pipettes
Pipet tips, assorted sizes
50 ml glass bottle
MQ water
Magnetic stirrer
CAS no
7601-90-3
5.
Supplier
Sigma
MW=
100.46
Name
70 % Perchloric acid
Volume 50 ml
4.3 ml
PROCEDURES: DESCRIPTION OF PROCEDURE
We always wear lab coat when working in the lab.
Specific making 6% perchloric acid use: 1) fume hood, 2) gloves and 3) safety glasses.
1.
2.
3.
4.
5.
Add approx. 40 ml with dH2O to a 50 ml glass bottle.
Transfer 4.3 ml 70 % perchloric acid to a 50 ml glass bottle.
Adjust the volume to 50 ml with dH2O (6 % perchloric acid).
Mix on magnetic stirrer.
Label the bottle with 6 % Perchloric acid and hazard symbol according to the risk conclusion
, date and your name and store in the fridge.
Postal address: UiO, Department of Biosciences (IBV), P.O. Box 1066 Blindern, N-0316 Oslo
Version: 002
S-SOP-xxx-shortname
6.
Page 2 of 4
RISK ASSESSMENT
The likelihood is assessed by assuming the user following the precautions stated in the step by step
risk assessment (SJA) below.
6.1 List of chemicals and their H and P phrases
Chemicals
symbol
Hazard
70% Perchloric acidi
H statements
P statements
H271 – May cause fire
or explosion; strong
oxidiser.
P210 : Keep away from heat, hot surfaces, sparks,
open flames and other ignition sources. No
smoking.
H290 – May be
corrosive to metals.
H302 – Harmful if
swallowed.
H314 – Causes severe
skin burns and eye
damage.
H373 – May cause
damage to organs
through prolonged or
repeated exposure
exposure cause the
hazard>.
P280: Wear protective gloves/ protective clothing/
eye protection/ face protection.
P303 + P361 + P353: IF ON SKIN (or hair): Take
off immediately all contaminated clothing. Rinse
skin with water/shower.
P304 + P340 + P310: IF INHALED: Remove
person to fresh air and keep comfortable for
breathing. Immediately call a POISON CENTER or
doctor/ physician.
P305 + P351 + P338 : IF IN EYES: Rinse
cautiously with water for several minutes. Remove
contact lenses, if present and easy to do. Continue
rinsing.
P371 + P380 + P375 : In case of major fire and
large quantities: Evacuate area. Fight fire remotely
due to the risk of explosion.
nh= This is not classified as harmful according to the Directive 67/548/EC
6.2 Risk assessment; step by step
Part of procedure
Unwanted
scenarios
Precautions
Emergency
planning
S*K
1-3
Spillage solution,
skin contact, eye
contact, respiratory
irritation, fire
Ensure that most of
the water is added to
the glass bottle
before the acid is
added to the bottle.
Locate the closest
sink and eye wash
station.
1*3
Adding 70 %
perchloric acid
See P statements
above
Wear gloves, safety
glasses, lab coat and
work in fume hood.
Check local fire
instructions
4-5
Adjusting volume
with water
Spillage may cause
eye and skin
irritation
Wear gloves, safety
glasses, lab coat and
work in fume hood.
P305 + P351 +
P338 : IF IN EYES:
Rinse cautiously
with water for
several minutes.
Remove contact
lenses, if present
and easy to do.
Continue rinsing.
P337 + P313: If eye
irritation persists:
Get medical
advice/ attention.
1*1
Version: 002
S-SOP-xxx-shortname
Page 3 of 4
6.3 Risk conclusion:
Risk categories;
 Red (10-25): Unacceptable risk. Assess new precautions to reduce the risk.
 Yellow (4-9): Assessing new precautions to reduce the risk should considered.
 Green (1-4): Acceptable risk.
If S*K of the step by step risk assessment falls into different categories (as listed above), the overall
risk is set to the highest S*K value.
In this SOP, there is MINIMAL RISK associated with making this solution, as in all steps S*K is less
than or equal to 4.
6.4 Labelling of new solution:
Chemicals
Hazard symbol
H statements
P statements
6% Perchloric acidii
Sigma also provides a 1M (6%) perchloric acid (34288) with the following H and P
statement
H315 – Causes skin
irritation.
P280: Wear protective gloves/ protective
clothing/ eye protection/ face protection.
H319 – Causes
serious eye irritation.
P305 + P351 + P338 : IF IN EYES: Rinse
cautiously with water for several minutes.
Remove contact lenses, if present and easy
to do. Continue rinsing.
P337 + P313: If eye irritation persists: Get
medical advice/ attention.
When using 6% perchloric acid gloves and safety glasses should be used, as 6% perchloric acid causes
skin and eye irritation
7.
WASTE DISPOSAL
When making this solution some waste is generated. It is important to access the potential risk this on
the environment and how this waste should be handled.
7.1 List of different wastes
Waste
Volume
Disposal method
Enviromental risk
1
0.1 – 100
ml
See chemical waste
procedyre (Farlig
avfall)
Chemicals for disposal are collected
twice a year. According to procedure
and handled with trained staff and
collected by professionals.
The risk waste is handled by
professionals
70 % perchloric acid
Disposal of chemical;
too much volume taken
out from bottle, the
stock solution of 70%
perchloric acid is out of
date etc.
2
Contaminated
disposables or small
chemical spillage with
70% perchloric acid;
0.1 – 1 ml
Risk waste
3
6 % perchloric acid
Rest of solution that is
too old.
up to 50 ml
Risk waste
Rest for using solution
to measure EtOH
concentration
This is rescribed in
separate SOP
-
S-SOP-xxx-shortname
8.
Version: 002
Page 4 of 4
REFERENCES
Sigma aldrich – MSDS:
http://www.sigmaaldrich.com/MSDS/MSDS/DisplayMSDSPage.do?country=NO&language=no&pro
ductNumber=311421&brand=ALDRICH&PageToGoToURL=http%3A%2F%2Fwww.sigmaaldrich.com
%2Fcatalog%2Fproduct%2Faldrich%2F311421%3Flang%3Den
i
ii
Sigma aldrich – MSDS:
http://www.sigmaaldrich.com/MSDS/MSDS/DisplayMSDSPage.do?country=NO&language
=ENgeneric&productNumber=34288&brand=FLUKA&PageToGoToURL=http%3A%2F%2Fww
w.sigmaaldrich.com%2Fcatalog%2Fproduct%2Ffluka%2F34288%3Flang%3Den