SAFE WORK PROCEDURE
LOCATION
VMC
WRITTEN BY: APPROVED BY:
Science Team
Making a Standard Solution
and a Dilution
Page 1 of 2
DATE CREATED
WS&H
LAST REVISION
June 27, 2014
New
PERSONAL PROTECTION EQUIPMENT (PPE)
Safety glasses or face shield must be worn at all
times in work areas.
Long and loose hair must be tied back
Appropriate footwear must be worn. Shoe must
be fully enclosed. No open toed shoes.
Close fitting/protective clothing must be worn.
Remove strings hanging from pullovers/sweaters.
Rings and jewelry (long necklaces / bracelets,
etc.) must not be worn.
HAZARDS PRESENT
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Chemical burns
Absorption of Chemicals.
Inhalation of Chemicals.
Ingestion of Chemicals.
Slips / trips & falls
Chemical hazards
ADDITIONAL
REQUIREMENTS
APPARATUS / MATERIALS
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0.01 g balance
Copper (II) chloride
Massing paper
Volumetric flasks
Distilled water
Micropipette
Stoppers
Beaker
10 ml pipette and pump
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Equipment orientation
WHMIS training
SAFE WORK PROCEDURE
1. Don all personal protective equipment including: safety glasses, & protective nitrile gloves (where
required). Ensure all loose clothing is either removed or tied back. Remove any jewelry and tie back
long hair.
2. Caution: Copper chloride can irritate the eyes and skin. Wear goggles at all times. Rinse skin
thoroughly after any contact. Copper chloride can also react with silver, so remove silver jewelry
before proceeding.
3. Calculate the mass of copper chloride two-hydrate to make 0.100L of 0.100M solution.
4. Put goggles on. If using massing paper, crease in the middle. Mass the amount of copper chloride twohydrate required for solution.
5. Carefully pour copper chloride in a 100 ml flask.
6. Squirt about 50 ml of distilled water into a flask, rinsing copper chloride off the sides.
7. Stopper the flask and swirl to dissolve copper chloride. Add more water if needed to dissolve copper
SAFE WORK PROCEDURE
Making a Standard Solution
and a Dilution
Page 2 of 2
chloride completely.
8. Fill the flask with distilled water almost up to the etching line, rinsing the sides. Stopper the flask and
swirl to mix. Use a micropipette to add distilled water exactly to the etching line. Put stopper in flask.
9. Bring flask with solution and clean, dry 100 ml beaker to teacher to be marked. Check volume. Invert
the flask a few times and then pour about 30 ml of solution into 100 ml beaker. Have conductivity
checked.
10. Assemble pipette by attaching pump to top of pipette.
11. Fill pipette with solution from beaker. Use dial to draw solution in. Use dial or lever to release solution.
Empty pipette into sink. (This rinses any other liquid out of the pipette).
12. Fill pipette from the beaker again and put exactly 10.0 ml of solution in 250 ml flask.
13. Take pump off pipette before lying pipette down.
14. Carefully fill flask with distilled water up to etching line. Put stopper in flask.
15. Wash 100 ml beaker with distilled water and dry thoroughly.
16. Bring flask with dilution and clean, dry 100 ml beaker to teacher to be marked.
17. Pour all solutions down the drain. Rinse flasks, pipette and beaker with distilled water. Return all
equipment to its proper place.
18. Clean up your workstation.
REGULATORY REQUIREMENTS
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WS&H Act W210, Section 4, 5
Mb. Regulations 217/2006,
Part 16, (Machines / Tools & Robots) Sections 16.1-16.18)
Part 35, (WHMIS Application)
Part 36, (Chemical & Biological Substances Application)