DATA: Genetic data for Sorghastrum nutans, aboveground net primary production (ANPP), soil carbon
and nitrogen accrual, and net nitrogen mineralization rates are contained in four spreadsheet files.
FILE: ISSR data for Sorghastrum nutans.xlsx
DETAILS: This file contains three separate sheets of data corresponding to the labeled tabs. Tab one (‘SN
ISSR diversity (freq data)’) corresponds to the seed source and plot level Percent Polymorphic (PPISSR)
and Shannon’s Diversity (H’ISSR) values. Tab two (‘Plot Freq (34 bands)’) corresponds to the plot level
band frequency data used to calculate Percent Polymorphic (PPISSR) and Shannon’s Diversity (H’ISSR). Tab
three (‘Individual full profiles (MRPP)’) corresponds to the individual plants for which we have complete
ISSR profile fingerprints. In this tab, the first column identifies the grass source (‘Rumsey’ Cultivar or
Local Ecotype) followed by the presence / absence (1/0) corresponding for all columns representing the
primer number and band number. For example, column p807b1 corresponds to primer 807 and band 1
for that specific primer. These data were used to the MRPP analysis.
FILE: ANPP_2006 to 2009_Figs 2 3 4.xlsx
DETAILS: The file contains data corresponding to Figures 2, 3, and 4. The data are grams of plant biomass
harvested at the end of the growing season in each year of the study (2006-2009) from 0.1 m2 frames,
multiplied by 10 (g m-2 y-1). Replicate frames (2-4) were harvested in each subplot. Biomass was
separated into each of the following categories: focal grasses of Andropogon gerardii, Sorghastrum
nutans, Schizachyrium scoparium, planted forbs, other planted grasses, volunteer forbs, volunteer
grasses, and previous year’s litter. Total ANPP, focal grass ANPP, total volunteer ANPP, and the ANPP of
all other planted species were calculated from the biomass categories. Previous year’s litter was not
included in the ANPP estimates. All but one ANPP category (“all other planted species”) were log-
transformed, “x + 1” if the data contained zero values, prior to statistical analyses.
Column A: BLOCK (1 and 2 corresponding to the pre-existing agricultural field use)
Column B: SOURCE (C=cultivar and L=local ecotype of the focal dominant grass corresponding to the
whole plot treatments)
Column C: SPPOOL (Species pool A, B, and C corresponding to the unique pool of non-focal grass species
corresponding to the subplot treatment)
Column D: YEAR (Year of measurement; 2006, 2007, 2008, and 2009 corresponding to the first, second,
third and fourth year of establishment)
Column E: PLOT (Whole-plot numbers assigned 1-12 corresponding to the 12 plots)
Column F: REPLICATE (biomass sample from within each SOURCE and SPPOOL treatment combination;
replicates were averaged prior to statistical analyses).
Column G: FOCALGR = sum of A. gerardii, S. nutans, and S. scoparium ANPP
Column H: ANGER = A. gerardii ANPP
Column I: SSCOP = S. scoparium ANPP
Column J: SORNUT = S. nutans ANPP
Column K: TOTAL = total ANPP (sum of all harvested biomass categories)
Column L: PLANTED = planted ANPP = FOCALGR + planted forbs + other planted grasses
Column M: VOLUNTEER = volunteer species ANPP = volunteer forb + volunteer grasses
Column N: ALLOTHPLANT = all other planted species ANPP = PLANTED – FOCALGR
Column O: PLANTFORB = ANPP of planted forbs
FILE: Soil C and N Stock_2006 2011.xlsx
DETAILS: This file contains the soil carbon and nitrogen stock in the 0-10 cm soil depth in equivalent
mass (g m-2) based on bulk density of soil in 2011.
Column A: BLOCK (1 and 2 corresponding to the pre-existing agricultural field use)
Column B: SOURCE (C=cultivar and L=local ecotype of the focal dominant grass corresponding to the
whole plot treatments)
Column C: SPPOOL (Species pool A, B, and C corresponding to the unique pool of non-focal grass species
corresponding to the subplot treatment)
Column D: PLOT (Whole-plot numbers assigned 1-12 corresponding to the 12 plots)
Column E: YEAR (year of measurement; 2006 and 2011 corresponding to the first and sixth year of
establishment)
Column F: EQmN = equivalent mass nitrogen stock
Column G: EQmC = equivalent mass carbon stock
FILE: Net N Mineralization Rate 2009.xlsx
DETAILS: This file contains net nitrogen (N) mineralization rate (ug g-1 d-1) data in the 0-5 cm soil depth
from each whole-plot and subplot treatment combination in 2009. Values represent the average of
three measurements. Data were log (x + 1000) transformed prior to statistical analysis.
Column A: BLOCK (1 and 2 corresponding to the pre-existing agricultural field use)
Column B: SOURCE (C=cultivar and L=local ecotype of the focal dominant grass corresponding to the
whole plot treatments)
Column C: SPPOOL (Species pool A, B, and C corresponding to the unique pool of non-focal grass species
corresponding to the subplot treatment)
Column D: PLOT (Whole-plot numbers assigned 1-12 corresponding to the 12 plots)
Column E: NMIN = net nitrogen mineralization rate
Column F: L_NMIN = log(NMIN + 1000)