New Phytologist Supporting Information Figs S1–S5 and Table S1
Integrating ecology and physiology of root-hemiparasitism: abiotic resources shape the
balance between root parasitism and shoot competition
Jakub Těšitel, Tamara Těšitelová, James P. Fisher, Jan Lepš and Duncan D. Cameron
Article acceptance date: 24 July 2014
The following Supporting Information is available for this article:
Fig. S1 Relative water content (in volumetric percent) in substrate of experimental pots
measured before the final irrigation of the experiment. Values for each pot are based on an
average of three subsequent reads. Box colours illustrate the irrigation and nutrient levels. See
Table S1 for a summary of corresponding mixed-effect model.
Fig. S2 Root biomass of the two host species cultivated under individual experimental
treatments. Bar colours illustrate the irrigation and nutrient levels. See Table S1 for a summary
of corresponding mixed-effect model.
Fig. S3 Pattern of the total above-ground biomass production in individual pots (i.e. sum of host
+ hemiparasite). Bar colours illustrate the irrigation and nutrient levels. See Table S1 for a
summary of corresponding mixed-effect model.
Fig. S4 Carbon stable isotope composition (δ13C) of bulk above-ground biomass of the two host
species cultivated under individual experimental treatments. Box colours illustrate the irrigation
and nutrient levels. See Table S1 for a summary of corresponding mixed-effect models.
Fig. S5 Carbon stable isotope composition (δ13C) of bulk above-ground biomass of Rhinanthus
alectorolophus attached to either of the host species and cultivated under individual
experimental treatments. Box colours illustrate the combinations of irrigation and nutrient
levels. See Table S1 for a summary of corresponding mixed-effect model.
Table S1 Summary of mixed-effect models describing the effects of host species identity, abiotic
resources and infection by the hemiparasite on the supplementary parameters of the
Irrigation
*** ↑
ns
ns
Nutrients
*** ↓
*** ↑
*** ↑
ns
ns
Infected
*** M
** ↑
ns
*↓
***↓
**↑
ns
*↑
Host×Irrigation
*
Host×Nutrients
Irrigation×Nutrients
Rhinanthus biomass δ13C
ns
Wheat biomass δ13C
*M
Maize biomass δ13C
Total above-ground
biomass production in the
pot
Host Species
Host root biomass
Relative water content in
substrate
experimental hemiparasitic associations.
***
ns
**
***
**
ns
Host×Infected
Irrigation×Infected
ns
Nutrients×Infected
**
***
Host×Irrigation×Nutrients
**
**
Host×Irrigation×Infected
Host×Nutrients×Infected
Irrigation×Nutrients×
Infected
*
Host×Irrigation×Nutrients×
Infected
***, P ≤ 0.001; **, P ≤ 0.01; *, P ≤ 0.05; ns, terms with non-significant regression slopes
retained in the model due to significant higher order interactions.
Empty and grey fields, terms omitted from the model during the model selection and terms
which are not available for particular model, respectively. Arrows, the direction of change of
response variable for statistically significant main effects (an arrow upwards or downwards
indicates that a response variable increases or decreases with that factor, respectively); M, the
host species (maize) associated with the higher values of response variable.
Methods S1 R code of model selection and summaries for the results reported in the main text.
Methods S2 R code of model selection and summaries for the results reported in the
Supporting Information.