Name: __________________________________ Period: ______ Lab: Assembling and Explaining Meiosis Introduction Cell division is how organisms grow and repair themselves. It is also how they produce offspring. Many single-celled organisms reproduce by binary fission. The parent cell simply divides to form two daughter cells that are identical to the parent. In many other organisms, two parents are involved, and the offspring are not identical to the parents. In fact, each offspring is unique. The children resemble their parents, but they are not identical to them. Instead, each has a unique combination of characteristics inherited from both parents. Sexual reproduction involves two parents. In sexual reproduction, parents produce reproductive cells—called gametes—that unite to form an offspring. Gametes are haploid cells. This means they contain only half the number of chromosomes found in other cells of the organism. Gametes are produced by a type of cell division called meiosis. The process in which two gametes unite is called fertilization. The fertilized cell that results is referred to as a zygote. A zygote is diploid cell, which means that it has twice the number of chromosomes as a gamete. The process that produces haploid gametes is meiosis. Meiosis is a type of cell division in which the number of chromosomes is reduced by half. It occurs only in certain special cells of the organisms. During meiosis, homologous chromosomes separate, and haploid cells form that have only one chromosome from each pair. Two cell divisions occur during meiosis, and a total of four haploid cells are produced. The two cell divisions are called meiosis I and meiosis II. The overall process of meiosis is summarized in the Figure below. 1 Part One: Assembling Meiosis Materials Compass 2 Large sheets of white paper (use the one from Colored pencils Mitosis lab and a blank new sheet) Pencil 4 different colors of pipe cleaners (use your Computer: Textbook (OL: pgs. 18-21; Chromosomes from the Mitosis lab); additional strips HC: pgs. 12-14 , PowerPoint (slides 30Of same colored pipe cleaner to assemble 34; NOTE THESE PICTURES DO NOT crossing-over ALL SHOW 4 CHROMOSOMES) 4 round beads (centromeres from Mitosis lab) 1 Ziploc bag with Mitosis materials in 2 3-pronged beads (centrioles from Mitosis lab) it 1 Bead necklace (nuclear membrane/ envelope from Mitosis lab) Scissors Ruler 1. Assign the tasks in the chart below to each group member. You will be evaluating each other’s contributions when the lab is complete. Tasks 1. Gather groups computers 2. Gather materials needed 3.Assemble chromosome pieces, nuclear membrane pieces, and additional pipe cleaners that will be needed 4. Draw phase circles on large white sheet & label 5. Answer questions on pages 1 & 3 of the lab Group Member Assigned 1. 2. 3. 4. 5. ALL GROUP MEMBERS COLLABORATIVELY 2. Cut 1 piece of pipe cleaner 1” long from each color of pipe cleaner. You will end up with four 1” sections of each color. DO NOT DISCARD YOUR EXTRA PIECES OF PIPE CLEANER. 3. You will pencil-draw in the spindles where needed. 4. You will need to re-label your sheet from the Mitosis lab as shown below: Meiosis I Prophase I Anaphase I Cytokinesis I Metaphase I Telophase I 2 5. On your second white sheet of paper (in pencil) draw & label it as shown below: a. 4 Large circles under meiosis II should be drawn with your compass and have a 3 ½ ” diameters b. The smaller circles of cytokinesis II should have 2 ” diameters Meiosis II Prophase I I Telophase I I Metaphase I I Anaphase I I Cytokinesis I I 6. Now in the chart below assign each person in your group a phase of mitosis: Phase Group Member 1. Prophase I 2. Metaphase I 3. Anaphase I 4. Telophase I Cytokinesis I 5. Prophase II 6. Metaphase II 7. Anaphase II 8. Telophase II Cytokinesis II 7. You will need to show all four sister chromatids crossing-over. Use your resources to remember which phase crossing-over happens in. 8. Take turns practicing and explaining what happens in each of your assigned phases using the materials provided on the large sheets of white paper. You may use your textbooks to help you. When you think you are ready call an instructor over so that as a group you can demonstrate and explain Meiosis I, Meiosis II, Cytokinesis I, and Cytokinesis II; scores will be recorded on the rubric on page 7 of this lab. 9. You are now ready to complete Part Two below. 3 Part Two: After you have assembled and explained mitosis & cytokinesis to your instructor on the big sheet of white paper transfer that information (using colored pencils to match the colors of pipe cleaners you used) by drawing each phase’s activities onto the diagrams below. On the blank lines provided next to each phase; list the events of the each phase. MEIOSIS I: Prophase I 1. ______________________________________________________________ 2. ______________________________________________________________ 3. ______________________________________________________________ 4. ______________________________________________________________ 5. ______________________________________________________________ 6. ______________________________________________________________ Metaphase I 1. ______________________________________________________________ 2. ______________________________________________________________ Anaphase I 1. ______________________________________________________________ 2. ______________________________________________________________ 3. ______________________________________________________________ Telophase I 1. ______________________________________________________________ 2. ______________________________________________________________ 3. ______________________________________________________________ Cytokinesis I 1. _______________________________________________________ 2. _______________________________________________________ 3. _______________________________________________________ 4 MEIOSIS II: Prophase II 1. ______________________________________________________________ 2. ______________________________________________________________ 3. ______________________________________________________________ Metaphase II 1. ______________________________________________________________ 2. ______________________________________________________________ Anaphase II 1. ______________________________________________________________ 2. ______________________________________________________________ Telophase II 1. ______________________________________________________________ 2. ______________________________________________________________ Cytokinesis II 1. _______________________________________________________ 2. _______________________________________________________ 3. _______________________________________________________ 5 PART THREE: Conclusions and Analysis 1. Define gamete and zygote. What number of chromosomes does each have? ___________________________________________________________________________________________ ___________________________________________________________________________________________ ___________________________________________________________________________________________ 2. What happens during fertilization? ___________________________________________________________________________________________ ___________________________________________________________________________________________ 3. What is gametogenesis, and when does it occur? ___________________________________________________________________________________________ ___________________________________________________________________________________________ 4. Create a diagram (PICTURE) to show how crossing-over occurs and how it creates new gene combinations on each chromosome. 5. Explain why sexual reproduction results in genetically unique offspring. ___________________________________________________________________________________________ ___________________________________________________________________________________________ ___________________________________________________________________________________________ ___________________________________________________________________________________________ ___________________________________________________________________________________________ 6. Explain how meiosis I differs from mitosis. ___________________________________________________________________________________________ ___________________________________________________________________________________________ ___________________________________________________________________________________________ 6 SCORE ON PRESENTATION: Phase Prophase I 1 Only 1 event accurately explained and manipulated using the model Metaphase I Only 1 event accurately explained and manipulated using the model Only 1 event accurately explained and manipulated using the model Only 1 event accurately explained and manipulated using the model Only 1 event accurately explained and manipulated using the model Only 1 event accurately explained and manipulated using the model Anaphase I Telophase I Cytokinesis I Prophase II Metaphase II Anaphase II Telophase II Cytokinesis II Score Totals Final Score Only 1 event accurately explained and manipulated using the model Only 1 event accurately explained and manipulated using the model Only 1 event accurately explained and manipulated using the model Only 1 event accurately explained and manipulated using the model 2 Less than 6 events accurately explained and manipulated using the model Both events inaccurately explained and manipulated using the model Only 2 events accurately explained and manipulated using the model Only 2 events accurately explained and manipulated using the model Only 2 events accurately explained and manipulated using the model Less than 3 events accurately explained and manipulated using the model Both events inaccurately explained and manipulated using the model Both events inaccurately explained and manipulated using the model Both events inaccurately explained and manipulated using the model Only 2 events accurately explained and manipulated using the model 3 All 6 events accurately explained and manipulated using the model. Both events accurately explained and manipulated using the model. All 3 events accurately explained and manipulated using the model. All 3 events accurately explained and manipulated using the model. All 3 events accurately explained and manipulated using the model. All 3 events accurately explained and manipulated using the model. Both events accurately explained and manipulated using the model. Both events accurately explained and manipulated using the model. Both events accurately explained and manipulated using the model. All 3 events accurately explained and manipulated using the model. /15 7