Microhabitat Variability in Coho Overwintering Habitats on the Anchor River, AK prepared by Michael Callahan, Phd Candidate University of South Florida For The Kachemak Bay Research Reserve 95 Sterling Highway, Suite 2 Homer, AK 99603 Contact: Coowe Walker coowe.walker@alaska.gov 907.226.4651 Three habitats were chosen from the larger set of 25 coho overwintering sites. Habitats were selected to span a range of groundwater inputs and were selected based on presence of juvenile coho salmon and accessibility. The three habitats differed in terms of size, bathymetry, and inferred groundwater contribution, with groundwater contribution inferred from mass-balance mixing results. The high groundwater influence site, GW14, is a small and shallow off-channel or backwater habitat with a length of 40 meters (Figure 1). GW14 has one deeper pool located approximately at 33 meters downstream. In August 2012, the pool at GW14 had a water depth of approximately 0.7 meters (Figure 2). The middle groundwater influence site, GW03, is a large off-channel habitat with an approximate length of 120 meters (Figure 3). GW03 has a large and deep pool located between 50-80 m downstream. In August 2012, the pool at GW03 had a water depth of approximately 1.5 meters (Figure 4). GW03 also has a groundwater seep flowing into the off-channel habitat at approximately 45 meters downstream, discharging to the habitat just upstream of the large and deep pool. The low groundwater influence site, NG04, is a relatively small off-channel habitat extending 50 meters and is located in a meander bend of the Anchor River (Figure 5). Only a small gravel bar separates the off-channel habitat from the main river. NG04 has a small pool in the middle of the habitat located at 30 meters downstream. In August 2012, the pool at NG04 had a water depth of approximately 0.85 meters (Figure 6). High river stages in September 2012 severely modified the off-channel habitat at NG04 and wiped out the majority of the instrumentation. Therefore, no water quality parameters will be reported for NG04 in this report. Specific conductivity was elevated at both GW14 (Table 1) and GW03 (Table 2), indicating the influence of groundwater to both of these off-channel habitats. Mean specific conductivity was 122.6 (±41.7) μS/cm at GW14 and 81.7 (±13.8) μS/cm at GW03, supporting previous results of high groundwater influence at GW14 and moderate groundwater influence at GW03. Although groundwater contributed to both sites, the mechanisms and responses differed. Site GW14 had what appears to be diffuse groundwater discharge entering throughout the off-channel habitat. Conversely, site GW03 had focused groundwater discharge entering the off-channel habitat from a spring flowing off a hillslope and into the middle of the habitat. Surface-water temperatures at GW14 approached freezing (0°C) in winter and were generally consistent throughout the length of the off-channel habitat (Figure 7). All surfacewater temperatures recorded between 10/22/12 and 03/08/13 were less than 1°C. The dissolved oxygen concentrations varied considerable during the study period, at numerous times dipping below 8 mg/L (Figure 8). Surface-water temperatures at GW03 stayed relatively warm throughout the winter in comparison, with a noticeable increase in temperature near where the groundwater seep enters the off-channel habitat and the beginning of the pool (Figure 9). The concentration of dissolved oxygen also remained high throughout the winter (>8 mg/L) (Figure 10). It appears as if the focused groundwater discharge at GW03 elevates surface water temperatures during the winter and keeps a proportion of the off-channel habitat ice free, which allows dissolved oxygen concentrations to remain high throughout the winter. While GW14 has a high proportion of groundwater, it lacks an area of focused discharge to elevate water temperatures. Surface-water temperatures at GW14 will decrease to the point of freezing, which can cut off oxygen exchange with the atmosphere causing decreases in surface water dissolved oxygen. Figure 1. Contour plot of ground surface elevation (meters) for site GW14. Figure 2. Contour plot of August 2012 surface water levels (meters) for site GW14. Figure 3. Contour plot of ground surface elevation (meters) for site GW03. Figure 4. Contour plot of August 2012 surface water levels (meters) for site GW03. Figure 5. Contour plot of ground surface elevation (meters) for site NG04. Figure 6. Contour plot of August 2012 surface water levels (meters) for site NG04. Table 1. Water temperature (°C), dissolved oxygen (mg/L), and specific conductivity (μS/cm) for site GW14. Temperature (°C) 5m 8/23/12 8.2 8/27/12 10.2 10/17/12 1.5 10/22/12 0.5 10/26/12 0.4 3/8/13 0.1 19m 8.2 1.3 0.8 0.6 0.1 33m 8.4 10.1 1.7 0.8 0.8 0.1 45m 8.8 10.3 1.1 0.7 0.5 0.1 Well 1 9.5 10.6 Well 2 8.7 8.9 Well 3 9.7 9.4 Well 4 9.6 10.2 2.8 2.0 3.5 3.0 Frozen Frozen 0.8 0.7 33m 5.8 5.4 11.0 8.9 5.7 13.2 45m 7.2 4.8 13.1 7.5 7.8 13.4 Well 1 0.1 0.2 Well 2 0.5 0.5 Well 3 0.5 0.2 Well 4 0.1 0.2 0.3 0.5 0.4 1.3 Frozen Frozen 0.5 0.5 33m 115 108 139 155 161 50 45m 104 109 188 158 162 49 Well 1 127 113 Well 2 194 131 Well 3 129 113 Well 4 222 203 99 101 57 32 Frozen Frozen 119 140 DO (mg/L) 8/23/12 8/27/12 10/17/12 10/22/12 10/26/12 3/8/13 5m 7.3 5.8 13.0 7.5 5.5 13.4 SP Cond (μS/cm) 5m 8/23/12 103 8/27/12 109 10/17/12 136 10/22/12 156 10/26/12 161 3/8/13 49 19m 6.5 12.2 6.8 5.6 13.4 19m 105 136 156 159 50 Table 2. Water temperature (°C), dissolved oxygen (mg/L), and specific conductivity (μS/cm) for site GW03. Temperature (°C) 20m 8/23/12 8.8 8/27/12 9.0 10/16/12 3.2 10/23/12 1.4 10/26/12 1.2 3/12/13 1.6 42m 7.9 8.1 3.2 2.3 1.8 1.8 60m 7.8 8.0 3.6 2.8 2.4 2.6 77m 8.1 8.0 3.5 2.2 2.3 2.2 100m 8.1 8.0 3.6 1.6 1.9 2.1 120m 8.6 20m 12.3 10.1 15.2 15.2 15.7 9.2 42m 10.5 12.4 14.7 13.8 13.8 10.4 60m 11.5 12.3 13.7 13.6 13.6 8.6 77m 10.7 12.3 13.5 14.5 13.5 10.8 100m 9.4 12.0 13.2 13.0 12.4 10.3 120m 11.8 SP Cond (μS/cm) 20m 8/23/12 77 8/27/12 80 10/16/12 84 10/23/12 99 10/26/12 99 3/12/13 53 42m 76 77 85 99 93 58 60m 76 77 86 97 92 59 77m 76 78 86 98 92 59 100m 74 77 85 101 93 59 120m 78 3.4 1.8 2.2 2.2 Well 1 9.5 9.8 Well 2 7.9 8.6 Well 3 9.2 8.3 Well 4 11.3 11.6 Frozen Frozen 4.7 4.7 5.4 5.4 4.2 2.8 Well 1 0.1 0.2 Well 2 0.1 0.1 Well 3 6.7 6.1 Well 4 0.2 0.7 Frozen Frozen 0.4 0.3 0.4 3.3 0.5 0.2 Well 1 98 95 Well 2 266 280 Well 3 67 60 Well 4 121 114 Frozen Frozen 152 139 92 54 83 76 DO (mg/L) 8/23/12 8/27/12 10/16/12 10/23/12 10/26/12 3/12/13 13.2 14.1 13.3 10.2 85 100 92 58 12 Temperature (°C) 10 8 8/23/12 8/27/12 6 10/17/12 10/22/12 4 10/26/12 3/8/13 2 0 5m 19m 33m 45m Loca on (m) Figure 7. GW14 surface water temperature (°C) along the length of the off-channel habitat. 18 16 Dissolved Oxygen (mg/L) 14 12 8/23/12 10 8/27/12 10/17/12 8 10/22/12 6 10/26/12 4 3/8/13 2 0 5m 19m 33m 45m Loca on (m) Figure 8. GW14 dissolved oxygen concentration (mg/L) along the length of the off-channel habitat. 12 Temperature (°C) 10 8 8/23/12 8/27/12 6 10/16/12 10/23/12 4 10/26/12 2 3/12/13 0 20m 42m 60m 77m 100m 120m Loca on (m) Figure 9. GW03 surface water temperature (°C) along the length of the off-channel habitat. 18 16 Dissolved Oxygen (mg/L) 14 12 8/23/12 10 8/27/12 10/16/12 8 10/23/12 6 10/26/12 4 3/12/13 2 0 20m 42m 60m 77m 100m 120m Loca on (m) Figure 10. GW03 dissolved oxygen concentration (mg/L) along the length of the off-channel habitat.