Thomas D. Hock Ph.D.
________________________________________________________________________
CURRENT POSITION

Fall 2013-Present Employed as a Biology Instructor at The Marion Military
Institute Marion, Alabama
EDUCATION
2004 - 2007 The University of Alabama at Birmingham Birmingham, Alabama
Ph.D.
- Biochemistry and Molecular Genetics
Researched and defended dissertation titled “
Regulation of the Human Heme
Oxygenase-1 Gene”.
1995-1999 The Florida State University Tallahassee, Florida
M.S.
– Department of Biological Science
Researched and defended master’s thesis titled “ Cloning and Characterization of the E23
Ecdysone Induced Gene of Drosophila melanogaster.”
1992 - 1994 The Florida State University Tallahassee, Florida
B.S.
– Biological Science
Cum Laude
1989 - 1992 Daytona Beach Community College (now Daytona State College)
Daytona Beach, Florida
A.A.

RESEARCH EXPERIENCE
July 2010-March 2013
Research Associate in the lab of Dr. Victor Thannickal, Department of Pulmonary
Medicine. My primary duties include performing independent research on factors involved in idiopathic pulmonary fibrosis. This work involves characterization of the clusterin gene product and its effect on extracellular matrix deposition and degradation. I am also involved in studying the role of Nox4 in pulmonary fibrosis and its transcriptional mechanisms. In addition I do protocol development, antibody evaluation and lab maintenance.
July 2008-June 2010
Research Assistant in the lab of Dr. Clint Lothrop, Department of Biochemistry and
Molecular Genetics. Dr. Lothrop’s lab is researching the cyclic hematopoiesis phenomenon seen in canines. My work involved cloning and protein expression of the canine elastase cDNA, real time PCR and western blotting/detection of canine proteins, and development of enzymatic assays.
July 2007-July 2008
Post Doctoral scholar in the Department of Anesthesiology. Responsibilities include independently design, perform and interpret experiments relating to oxidative cell death by inhibiting HO-1 expression in cancer cell.
November 2002-June 2007
Employed at the University of Florida, Department of Medicine and then the University of Alabama at Birmingham, Department of Medicine, Division of Nephrology as a
Research Assistant in the laboratory of Anupam Agarwal M.D.
Research in Dr. Agarwal’s lab was centered on the regulation of the human Heme
Oxygenase (HO-1) gene. My research was focused on determining the sequences responsible for HO-1 induction in response to various stimuli, and to determine which factors are binding to these sequences and resolving these factors’ function in HO-1 induction.
June 1999-January 2002
Research Associate at the University of Kentucky Medical School, Department of
Pathology Lexington, Kentucky

Research and laboratory focus: Mammalian DNA repair; genetic traits of DNA repair related illnesses, hormonal regulation of genetic cascades in rat and mouse cells.
Duties: Perform DNA repair experiments on human and mouse cell lines using various techniques; in vitro construction of detected mutations from human samples and construction of stably transfected cell lines exhibiting these mutations; protocol development, maintain lab stocks and equipment, and general lab maintenance.
January 1995 – January 1999
Graduate School Research, Florida State University, Department of Biological Science.
As a graduate student, I was responsible for conducting and analyzing research on the
E23 ecdysone induced gene of Drosophila . In connection with this, I successfully cloned, sequenced and mapped cDNAs from the locus, determined the temporal and spatial expression of E23 throughout development using Northern hybridization of total RNA extracted from staged animals and analyzed its induction capabilities under various in vitro tissue culture conditions. I identified the E23 protein product on western blots using monoclonal antibodies that were produced by using four fusion proteins as antigens, which I had constructed, expressed and purified. I further made and studied E23 loss-offunction and gain-of-function mutants using transposon mutagenesis. I successfully developed techniques and protocols for the lab involving the purification of RNA, genomic DNA, northern and western blotting and hybridizations.
EXPERIENCE IN MOLECULAR BIOLOGY TECHNIQUES
My experience in molecular biology techniques includes but is not limited to the following:

Purification and quantitation of nucleic acids from genomic, plasmid, cosmid, P1 and lambda phage, mammalian cell cultures

Mammalian cell culture, cell cloning, generating transient and stably transfected cell lines

Analysis of genomic walks; gel electrophoresis; SDS PAGE; restriction mapping; intron-exon mapping and RFLP analysis

In vivo DMS footprinting

DNAse hypersensitive analysis

Chromatin Immunoprecipitation

Elisa

Kinetic enzyme assay development

Immunohistochemistry, Immunocytochemistry
 cDNA library development and screening; general and complex cloning; DNA sequencing

Site Directed Mutagenesis


PCR, long PCR, RT-PCR, qRT-PCR, plasmid rescue and primer extension

Southern, Northern and Western blotting

Fusion protein purification, fluorescent immunohistochemistry; in vitro culture and genetic analysis of Drosophila tissues

Lac Z reporter enhancer trapping; genetic manipulation of Drosophila cultures including recombinant analyses and transposon mutagenesis

Capturing Chromosome Conformation

Microscopy
TEACHING EXPERIENCE
August 2013-Present : Biology Instructor at The Marion Military Institute Marion,
Alabama. My duties are to instruct Bio 103, Bio 104, Bio 201, Bio 202 and Bio 220 classes. These are Biology I and II, Human Anatomy and Physiology I and II and beginning in Fall of 2015 Microbiology. Instruction includes preparation of lectures, laboratories, and grading materials.
August 2011-April 2013: Adjunct instructor at Bevill State Community College
I was employed by Bevill State as an adjunct instructor for the biology and anatomy and physiology courses offered at the Sumiton, Alabama campus. My responsibilities included lecture preparation and delivery, lab work preparation and design, test preparation and grading.
June 1999-2011 General laboratory teaching
Throughout my employment as a Research Associate and Postdoctoral Associate I have been responsible for teaching many undergraduate, graduate students and medical interns in the fields of molecular biology techniques, basic biology and anatomy, genetics, protein analysis, DNA/RNA analysis and purification, experimental design and data interpretation.
January 1995 – December 1998 Graduate teaching assistant
During the time I was employed by The Florida State University as a graduate teaching assistant I assisted in laboratory and lecture courses to junior and senior biology majors in the fields of invertebrate zoology, genetics, Drosophila laboratory genetics, molecular biology, gene cloning and protein biochemistry. My duties and responsibilities for these classes included preparation and delivery of lectures, conducting weekly review sessions, preparing and grading examinations and lab practical exams, demonstrating and overseeing molecular biology laboratory work. In addition, I was responsible for teaching work-study students Drosophila genetics and molecular analyses of Drosophila cultures.

August 1993 – April 1996 Non-major biology lab/lecture teacher
During this time, I attended and graduated from the teaching workshop for senior and graduate biology students. I instructed six sections with up to twenty-four students per section of non-major biology labs. Topics for this course included fundamental genetics, zoology, fundamental cell biology, and organismal biology.
Honors and Awards

Recipient of the 2005 Samuel B. Barker Award, Trainee Research Symposium,
Department of Medicine at the University of Alabama at Birmingham for excellence in research by a graduate student.

Recipient of the Hall of Fame award at Daytona Beach Community College,
Daytona Beach, Florida. (Now The Daytona State College)
Peer Reviewed Publications
Louise Hecker, Naomi J. Logsdon, Deepali Kurundkar, Ashish Kurundkar, Karen
Bernard, Thomas Hock , Eric Meldrum, Yan Y. Sanders, and Victor J.
Thannickal. Reversal of Persistent Fibrosis in Aging By Targeting the Nox4-Nrf2
Redox Imbalance. Accepted for Publication. Science Translational Medicine .
January 2014.
Bai G, Hock TD , Logsdon N, Zhou Y, Thannickal VJ. A far-upstream AP-1/Smad binding box regulates human NOX4 promoter activation by transforming growth factor-β. Gene. 2014 Feb 20. pii: S0378-1119(14)00214-5
Karki S, Surolia R, Hock TD , Guroji P, Zolak JS, Duggal R, Ye T, Thannickal VJ,
Antony VB. Wilms' tumor 1 (Wt1) regulates pleural mesothelial cell plasticity and transition into myofibroblasts in idiopathic pulmonary fibrosis. FASEB J.
2013 Nov 21. [Epub ahead of print] PMID:24265486
Zhang X, Liu H, Hock T , Thannickal VJ, Sanders YY. Histone deacetylase inhibition downregulates collagen 3A1 in fibrotic lung fibroblasts. Int J Mol Sci. 2013 Sep
27;14(10):19605-17 .
Vallejo MO, Niemeyer GP, Vaglenov A, Hock T , Urie B, Christopherson P, Lothrop CD
Jr. Decreased hematopoietic progenitor cell mobilization in pearl mice. Exp
Hematol. 2013 Oct;41(10):848-57

Zolak JS, Jagirdar R, Surolia R, Karki S, Oliva O, Hock T , Guroji P, Ding Q, Liu RM,
Bolisetty S, Agarwal A, Thannickal VJ, Antony VB.
Pleural Mesothelial Cell Differentiation and Invasion in Fibrogenic Lung Injury .
Am J Pathol . 2013 Feb 9.
Deshane, J., Kim, J., Bolisetty, S., Hock, T. D ., Hill-Kapturczak, N., and Agarwal, A.,
Sp1 regulates chromatin looping between an intronic enhancer and distal promoter of the human heme oxygenase-1 gene in renal cells, J Biol Chem, 285 ,
16476 (2010).
Meng R, Bridgman R, Toivio-Kinnucan M, Niemeyer GP, Vernau W, Hock T , Lothrop
CD, Jr. 2010. Neutrophil elastase-processing defect in cyclic hematopoietic dogs.
Exp Hematol 38(2):104-115 .
Wright MM, Kim J, Hock TD , Leitinger N, Freeman BA, Agarwal A. 2009. Human haem oxygenase-1 induction by nitro-linoleic acid is mediated by cAMP, AP-1 and E-box response element interactions. Biochem J 422(2):353-361 .
Traylor A, Hock T , Hill-Kapturczak N. 2007. Specificity protein 1 and Smad-dependent regulation of human heme oxygenase-1 gene by transforming growth factor-beta1 in renal epithelial cells. Am J Physiol Renal Physiol 293(3):F885-894.
Deshane, J., Chen, S., Caballero, S., Grochot-Przeczek, A., Was, H., Li Calzi, S., Lach,
R., Hock, T. D ., Chen, B., Hill-Kapturczak, N., Siegal, G. P., Dulak, J.,
Jozkowicz, A., Grant, M. B., and Agarwal, A., Stromal cell-derived factor 1 promotes angiogenesis via a heme oxygenase 1-dependent mechanism, J Exp
Med, 204 , 605 (2007).
Hock TD , Liby K, Wright MM, McConnell S, Schorpp-Kistner M, Ryan TM, Agarwal A.
2007. JunB and JunD regulate human heme oxygenase-1 gene expression in renal epithelial cells. J Biol Chem 282(9):6875-6886 .
Kelley EE, Hock T , Khoo NK, Richardson GR, Johnson KK, Powell PC, Giles GI,
Agarwal A, Lancaster JR, Jr., Tarpey MM. 2006. Moderate hypoxia induces xanthine oxidoreductase activity in arterial endothelial cells. Free Radic Biol Med
40(6):952-959.
Mark A, Hock T , Kapturczak MH, Agarwal A, Hill-Kapturczak N. 2005. Induction of heme oxygenase-1 modulates the profibrotic effects of transforming growth factor-beta in human renal tubular epithelial cells. Cell Mol Biol (Noisy-le-grand)
51(4):357-362.
Liby K, Hock T , Yore MM, Suh N, Place AE, Risingsong R, Williams CR, Royce DB,
Honda T, Honda Y, Gribble GW, Hill-Kapturczak N, Agarwal A, Sporn MB.
2005. The synthetic triterpenoids, CDDO and CDDO-imidazolide, are potent

inducers of heme oxygenase-1 and Nrf2/ARE signaling. Cancer Res 65(11):4789-
4798.
Hock TD , Nick HS, Agarwal A. 2004. Upstream stimulatory factors, USF1 and USF2, bind to the human haem oxygenase-1 proximal promoter in vivo and regulate its transcription. Biochem J 383(Pt 2):209-218 .
Sikorski EM, Hock T , Hill-Kapturczak N, Agarwal A. 2004. The story so far: Molecular regulation of the heme oxygenase-1 gene in renal injury. Am J Physiol Renal
Physiol 286(3):F425-441.
Mellon I, Hock T , Reid R, Porter PC, States JC. 2002. Polymorphisms in the human xeroderma pigmentosum group A gene and their impact on cell survival and nucleotide excision repair. DNA Repair (Amst) 1(7):531-546.
Hock T , Cottrill T, Keegan J, Garza D. 2000. The E23 early gene of Drosophila encodes an ecdysone-inducible ATP-binding cassette transporter capable of repressing ecdysone-mediated gene activation. Proc Natl Acad Sci U S A 97(17):9519-9524 .