Congress Workshops 15-16 April, 2015 Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Molecular genetic test in infertility Workshop (Research and Clinical Center for Infertility) 15, 16 April 2015 15 April 2015 08:00-10:00 Sheikhha MH, M.D. Application of molecular tests in diagnosis and approach to infertility (Scientific Chairman) 10:00-10:30 Coffee break 10:30-12:30 DNA extraction by kit 12:30-13:30 Prayer & Lunch 13:30-17:00 DNA extraction by salting out method 16 April 2015 08:00-12:30 PCR 12:30-13:30 Prayer & Lunch 13:30-15:30 Gel electrophoresis 15:30-17:00 Discussion Fluorescence in situ hybridization (FISH) and applications in infertility Workshop (Research and Clinical Center for Infertility) 15, 16 April 2015 15 April 2015 08:00-09:30 Kalantar SM, Ph.D. Theory of FISH technique and applications in infertility (Scientific Chairman) FISH Indications for diagnosis 09:30-10:00 Coffee break Slide Preparation Slide Checking 10:00-13:00 Pretreatment of Slides Denaturation Hybridization Overnight Incubation 16 April 2015 Washing after Hybridization 08:30-09:30 Counter Staining Incubation 09:30-10:00 Coffee break Introduction of Imaging software 10:00-12:00 Introduction of fluorescence microscope Montazeri F, M.Sc., Ph.D. candidate Image Capturing Image Analysis 12:00-13:00 Applications in PND & PGD Question and Answering Micro TESE Workshop (Research and Clinical Center for Infertility) 16 April 2015 08:00-08:10 Opening session 08:10-08:30 Vahidi S, M.D. (Scientific Chairman) Workshop program 08:30-09:00 Moein MR, M.D. 09:00-09:30 Sedighi Gilani MA, M.D. 09:30-10:00 Salehi P, M.D. 10:00-10:30 10:30-11:15 Moein MR, M.D. 11:15-12:15 Vahidi S, M.D. Definition of Indication) micro-TESE (History Micro-TESE complications Genetic assessments before of micro-TESE Coffee break Practical techniques Live surgery 12:15-13:00 Discussion 13:00-14:00 Prayer & Lunch & Low-temperature transportation and cryopreservation of ovarian tissue & technology of CAM-xenotransplantation of ovarian tissue Workshop (Research and Clinical Center for Infertility) 16 April 2015 08:00-08:15 Opening session 08:15-08:45 Isachenko E, Ph.D. (Germany) Technology of 23-hours low-temperature transportation, preparation and freezing of ovarian tissue of patient W. (mother of first baby): detailed information 08:45-09:15 Isachenko V, Ph.D. (Germany) Six years research: modernized technology for ovarian tissue cryopreservation 09:15-10:00 Isachenko V, Ph.D. (Germany) Film with comments: Year 2012. modernized technology of preparation, freezing, thawing and in vitro culture of human ovarian tissue 10:00-10:30 Coffee break 10:30-13:00 Isachenko E, Ph.D. (Germany) Isachenko V, Ph.D. (Germany) Hands-on Long-term transport of ovarian tissue. Removal of GV-Oocytes (puncture of follicles) in fresh ovarian tissue before cooling as element of concept of our cryobank. Preparation of strips of ovarian tissue: release of cortex from medulla. One-day cooling of ovarian tissue as an important element of cryopreservation protocol. Freezing of strips in 5ml Cryo-Vials with different freezing constructions. Quick thawing of ovarian tissue in boiling water and removal of cryoprotectants In vitro culture of ovarian tissue in large volume by agitation 13:00-14:00 Prayer & Lunch 14:00-15:00 Isachenko V, Ph.D. (Germany) CAM- xenotransplantation of ovarian tissue 15:00-16:00. Isachenko V, Ph.D. (Germany) Film with comments: technology of CAMxenotransplantation of ovarian, testicular and cancer tissues 16:00-17:00 Isachenko E, Ph.D. (Germany) Isachenko V, Ph.D. (Germany) Hands on Storage and culture of eggs. Manufacturing and preparation of silicon rings. Drilling of eggs and cutting a window. Placing the silicon rings on CAM surface and transfer of tissues and tumor cells. 17:00-17:30 Discussion Embryo Selection Workshop (Research and Clinical Center for Infertility) 16 April 2015 08:00-08:15 Opening session 08:15-08:30 Khalili MA, Ph.D. (Scientific Chairman) Workshop program 08:30-09:00 Safari S, Ph.D. Candidate Embryo culture media 09:00-09:20 Ashourzadeh S, Ph.D. Candidate Morphologic assessment of embryo from cleavage to blatocyst 09:20-09:40 Faramarzi A, Ph.D. Candidate Application of embryo time_laps in ART program 09:40-10:00 Safari S, Ashourzadeh S, Halvaei I, Faramarzi A, Ph.D. Candidates Trouble shooting panel Embryo culture media & Development 2PN to Blastocyst 10:00-10:15 Coffee break 10:15-12:00 Halvaei I, Candidates Faramarzi A, Ph.D. embryo Practical session Embryo morphology assessment & noninvasive method for embryo selection 12:00-13:30 Safari S, Ph.D. Candidate Practical session, embryo culture media 13:30-14:30 Prayer & Lunch Embryo Transfer Workshop (Shahid Sadoughi Hospital, Hall B) 17April 2015 14:00-16:00 Full Echo Pro Dr. Marc Even • Embryogyn / Embryoview (Prof. Frydman Assistant) • Frydman Echo • Set TDT 6th Yazd International Congress and Student Award in Reproductive Medicine April 17-19, 2015 Shahid Sadoughi Hospital, Dr. Javadi Hall 17 April 2015, Friday Opening Session 09:00-09:05 09:05-09:15 Mirjalili MR, M.D. (University Chancellor) 09:15-09:20 Aflatoonian A, M.D. (Congress Chairman) 09:20-09:30 Khalili MA, Ph.D. (Scientific Chairman) Holy Quran and Iranian National Anthem Welcome note Welcome note Scientific Program First Scientific Session: General Session Chairpersons: Akbarian A, M.D.; Gourabi H, Ph.D.; Mojibian J, M.D.; Vahidi S, M.D.; Aflatoonian R, M.D., Ph.D. 09:30-09:40 Agha-Rahimi A, Ph.D. Winner (Category I) (Iran) 09:40-09:50 Amjadi FS, Ph.D. Candidate Winner (Category I) (Iran) Artificial seminal fluid preserves human sperm quality in vitrification program: An electron microscopy study Proteomic profile of human endometrium in normal compare to polycystic ovarian syndrome 09:50-10:10 Frydman R, M.D. Past, present and future of human reproduction (France) 10:10-10:30 Papanikolaou E, M.D. (Greece) 10:30-10:40 Montazeri M, Ph.D. Candidate Winner (Category I) (UK) Individualization of HCG in ART for oocyte maturation & Luteal phase support Activation of Toll-like receptor 3 reduces actin polymerization and adhesion molecule expression in endometrial cells, a potential mechanism for viral-induced implantation failure 10:40-10:50 Bazrgar M, Ph.D. Candidate Winner (Category II) (Iran) Preimplantation response to genome instability and prenatal status of genome integrity 10:50-11:00 Golestan Jahromi M, Ph.D. Candidate Winner (Category II) (Iran) New insight into endometriosis pathogenesis, recurrence and treatment approch 11:00-11:30 Coffee Break, Exhibition & Poster visiting 17 April 2015, Friday Panel I Panel Coordinators: Aflatoonian A, M.D. (Iran); Frydman R, M.D. (France) 11:30-11:40 Nemati Dehkordi M, M.D. (Iran) The improvement of IVF outcome: A new approach 11:45-13:00 How to increase success rate in ART program Panel Members: Aleyasin A. M.D. (Iran) Al-Hasani S, Ph.D. (Germany) Arefi S, M.D. (Iran) Ashrafi M, M.D. (Iran) Kariminejad R, Ph.D. (Iran) Moieni A, M.D. (Iran) Papanikolaou E, M.D. (Greece) Parsanezhad ME, M.D. (Iran) Salehpour S, M.D. (Iran) Taheripanah R, M.D. (Iran) 13:00-14:00 Lunch Break, Pray, Exhibition & Poster visiting Second Scientific Session: General Session Chairpersons: Abdollahi-Fard S, M.D.; Ahmadi M, M.D.; Azhar H, M.D.; Ghahiri A, M.D.; Kazemeyni SM, M.D.; Mansouri M, M.D.; Shahverdi AH, Ph.D. 14:00-14:10 Arabjahvani F, M.D. Winner (Category III) (Iran) 14:10-14:20 Raberi A, Ph.D. Candidate Winner (Category II) (UK) 14:20-14:40 Papanikolaou E, M.D. (Greece) 14:40-14:50 Ghazali Sh, Ph.D. Candidate Winner (Category III) (Iran) 14:50-15:00 Lashkari BS, Ph.D. Candidate Winner (Category III) (UK) 15:00-15:10 Tarighi SH, D.V.M. Winner (Category III) (Iran) 15:10-15:20 Artimani T, Ph.D. Candidate Winner (Category IV) (Iran) 15:20-15:30 Ashourzadeh S, Ph.D. Candidate Winner (Category IV) (Iran) Transvaginal endometrial perfusion of granulocyte colony-stimulating factor for infertile women with thin endometrium in frozen embryo transfer program Comprehensive chromosome screening of single sperm using a whole genome sequencing technique Improving success in ART by Individualization of ovarian stimulation Efficacy of motile sperm organelle morphology examination (MSOME) and sperm head vacuoles evaluation in conventional IVF versus ICSI cycles Toll-like receptor 2 and 4 expression in the pregnant and non-pregnant human uterine cervix The Viability Rate of Ovine Spermatogonial Stem Cells after Cryopreservation in Different Concentrations of FBS Association between Nuclear receptors of estrogen and progesterone with adiponectin receptors in granulosa cells of patients with Polycystic Ovary Syndrome GDF-9 supplementation improved formation rates in clinical IVM program 15:30-16:00 Coffee Break, Exhibition & Poster visiting embryo 17 April 2015, Friday Third Scientific Session: General Session Chairpersons: Akhondi MM, Ph.D.; Maadani T, M.D.; Dehghani Firouzabadi R, M.D.; Ghafourzadeh M, M.D.; Mohammadian F, M.D.; Nematollahi-mahani SN, Ph.D.; Pourmand Gh, M.D. 16:00-16:10 Farid-Mojtahedi M, M.D. Winner (Category IV) (Iran) Effect of single dose GnRH agonist on pregnancy outcome in frozen-thawed embryo transfer cycles 16:10-16:20 Ghasemzadeh J, M.Sc. Winner (Category IV) (Iran) Sperm chromatin condensation, DNA fragmentation and apoptosis in globozoospermic patients 16:20-16:30 Safari S, Ph.D. Candidate Winner (Category IV) (Iran) Does cosmetic micromanipulation of vitrifiedwarmed preimplantation embryos enhance pregnancy rate? 16:30-16:40 Yasini SM, M.D. (Iran) Factors associated with adoption acceptance rate from the view point of infertile couples 16:40-16:50 Mahdian S, Ph.D. Candidate (Iran) Macrophage migration inhibitory factor as a potential biomarker of endometriosis 16:50-17:00 Hosseini E, Ph.D. Candidate (Iran) Expression and epigenetic alterations of aromatase coding gene, CYP19A1, in cumulus cells of infertile endometriosis patients 17:00-17:10 Zandieh Z, Ph.D. (Iran) Evaluation of immunological interaction between spermatozoa and fallopian tube epithelial cells 17:10-17:20 Abdollahi M, Ph.D. (Iran) Evaluation of in vitro growth and apoptosis incidence in vitrified human ovarian tissue following treatment with growth differentiating Factor 9B (GDF-9B) and Leukemia Inhibitory Factor (LIF) 17:20-17:30 Dehghani MR, Ph.D. (Iran) Testis development in the absence of SRY: chromosomal rearrangements at SOX9 and SOX3 Award Ceremony 17:30-19:30 Award ceremony 18 April 2015, Saturday First Scientific Session: Obstetrics & Gynecology, Infertility Chairpersons: Karimzadeh Meybodi MA, M.D.; Akbari Asbagh F, M.D.; Chaychian Sh, M.D.; Ghandi S, M.D.; Mehdizadeh A, M.D.; Oskouian H, M.D. Kalantari A, M.D., Khalili MB, Ph.D. 8:00-8:10 Holy Quran 08:10-08:30 Rahimi M, M.D. (Australia) 08:30-08:50 Al-Hasani S, Ph.D. (Germany) 08:50-09:10 Fatemi H, M.D. (UAE) 09:10-09:30 Antsaklis A, M.D. (Greece) 09:30-09:50 Karimzadeh Meybodi MA, M.D. (Iran) 09:50-10:10 Asefjah H, M.D. (Iran) 10:10-10:25 Wahaaj MH, M.D. (Afghanistan) 10:25-10:45 Garmaroudi Sh, Ph.D. (Germany) Surgical or medical treatment for unruptured interstitial (cornual) ectopic pregnancy? That is the question Ultra rapid freezing “Vitrification” is the right tool for cancellation of fresh embryo transfer Sociocultural influences on fertility in the Middle East: the role of parental consanguinity, obesity and vitamin D deficiency “U/S Screening in Obstetrics in Perinatal Medicine. What, when and by who” Hysteroscopy in infertility Different presentations of endometriosis First and aneuploidy second trimester screening for Religion, Law and Ethics: An Ethical and Anthropological Reflection on Assisted Reproduction 10:45-11:15 Coffee Break, Exhibition & Poster visiting Panel I Panel Coordinator: Merghati- Khoei ES, Ph.D. (Iran) 11:15-11:30 Merghati- Khoei ES, Ph.D. Sexual Dysfunction (Iran) 11:30-13:00 Sexual Dysfunction & Assisted Reproduction Bioethics Panel Members: Garmaroudi Sh, Ph.D. (Germany) Salehi P, M.D. (Iran) Yasini SM, M.D. (Iran) 13:00-14:00 Lunch Break, Pray, Exhibition & Poster visiting 18 April 2015, Saturday Second Scientific Session: Uro-Andrology Chairpersons: Amir-Arjmand MH, M.Sc.; Hosseini SJ, M.D.; Noori M, Ph.D.; Nowroozi MR, M.D.; Sadrkhanloo RA, Ph.D. ; Tizro GhR, M.D. 14:00-14:15 Moein MR, M.D. Antioxidant and male infertility (Iran) 14:15-14:30 Sadeghi MR, Ph.D. The sperm aging: Is it affecting ART outcomes? (Iran) 14:30-14:45 Talebi AR, Ph.D. New markers in male fertility evaluation (Iran) 14:45-15:05 Cologne technology of cryoprotectant-free Isachenko E, Ph.D. vitrification of human spermatozoa: description (Germany) and effectiveness 15:05-15:20 Exogenous HSPA8 prolongs sperm survival by Moein Vaziri N, Ph.D. enhancing membrane fluidity in a cholesterol(Iran) dependent manner 15:20-15:30 Effect of 655 nm diode LASER irradiation on Esmaeili L, M.Sc. human sperm cell motility and ROS (Reactive (Iran) Oxygen Species) production 15:30-15:40 Effect of Melatonin on cryopreservation Asadi E, Ph.D. Candidate induced oxidative stress and apoptosis in (Iran) human spermatozoa 15:40-16:00 Coffee Break, Exhibition & Poster visiting Third Scientific Session: Fertility Preservation Chairpersons: Anvari M, Ph.D.; Eftekhar M, M.D.; Habibzedeh V, M.D.; Movahedin M, Ph.D.; Nikoukar H, M.D., Ph.D.; Rashidi B, M.D. 16:00-16:10 Effect of Human Ovarian Tissue Shams Mofarah Z, Ph.D. Candidate Vitrification/Warming on the Expression of (Iran) Genes Related to Folliculogenesis 16:10-16:30 Pluripotent stem cells and regenerative Veiga A, Ph.D. medicine (Spain) 16:30-16:50 Next steps towards the transplantable artificial Amorim CA, Ph.D. ovary (Belgium) 16:50-17:10 Cologne technology of cryopreservation of Rahimi G, Ph.D. human ovarian tissue: first in Germany baby(Germany) born, description and effectiveness 17:10-17:25 Salehnia M, Ph.D. Mitochondria and oocyte maturation (Iran) 19 April 2015, Sunday First Scientific Session: Embryology Chairpersons: Amirheydari AH, M.D.; Hoseini A, Ph.D.; Nikzad H, Ph.D.; Nottola SA, Ph.D.; Vosoogh A, M.D. 8:00-8:10 Holy Quran 08:10-08:20 Fertility preservation of young women with Karimi-Zarchi M, M.D. endometrial carcinoma or complex atypical (Iran) hyperplasia: case series and literature review 08:20-08:30 Cosmetic micromanipulation of preimplantation Halvaei I, Ph.D. Candidate embryos enhances pregnancy rate in patients with (Iran) previous implantation failure 08:30-08:50 Organelle morphodynamics in human mature Nottola SA, Ph.D. oocytes after cryopreservation: Ultra structural (Italy) analysis at different time intervals during thawing 08:50-09:10 Ultra structural markers of aging in human Macchiarelli G, Ph.D. oocytes (Italy) 09:10-09:20 Cytoplasmic transplantation in oocytes obtained Soleimani R, Ph.D. from ovarian tissue xenotransplantation lead to (Belgium) higher fertilization rate 09:20-09:30 Reducing the risks in producing tissue engineered Salehi Moghaddam Z, M.Sc. buccal mucosa as a bladder graft (Iran) 09:30-09:45 Manipulation of human oocytes and embryos to Ghaffari Novin M, Ph.D. diagnose and treat from point of embryologist (Iran) view 09:45-10:05 Foreseeing the fate of the embryo advances and Losonczi EK, Ph.D. limitations of time-lapse technology Vitrolife Co. (Hungary) In vitro culture of human embryos with micro10:05-10:25 vibration: effectiveness established on 25000 Isachenko V, Ph.D. embryos from 5000 patients, description of (Germany) method and demonstration of new German apparatus 10:25-11:00 Coffee Break, Exhibition & Poster visiting Panel I Panel Coordinator: Khalili MA, Ph.D. (Iran) 11:00-11:15 Cryopreservation in ART Khalili MA, Ph.D. (Iran) 11:30-13:00 Cryopreservation in ART Panel Members: Al-Hasani S, Ph.D. (Germany) Amorim CA, Ph.D. (Belgium) Isachenko E, Ph.D. (Germany) Isachenko V, Ph.D. (Germany) Nottola SA, Ph.D. (Italy) Salehnia M,Ph.D. (Iran) 12:30-14:00 Lunch Break, Pray, Exhibition & Poster visiting 19 April 2015, Sunday Second Scientific Session: Stem Cell & etc. Chairpersons: Aflatoonian B, Ph.D.; Amiri I, Ph.D.; Davar R, M.D.; Hashemitabar M, Ph.D.; Jalali M, Ph.D.; Yousefnejad F, M.D. 14:00-14:10 Morphology and apoptosis evaluation in long Abdi Sh, Ph.D. term cultures of vitrified mouse whole ovaries (Iran) in the percent of LIF 14:10-14:20 Relationship between equilibration times and Taheri F, Ph.D. Candidate the presence of cumulus cells for vitrification of (Iran) in vitro matured ovine oocytes 14:20-14:35 Nasr-Esfahani MH, Ph.D. Advances in cloning technology (Iran) 14:35-14:50 Esfandiari F,Ph.D. Stem cells for infertility treatment (Iran) 14:50-15:10 Skutella T, Ph.D. Spermatogonial stem cells (Germany) The most important challenges and interventional strategies in providing 15:10-15:20 Reproductive Health services for prevention, Farajkhoda T, Ph.D. diagnosis and treatment of Sexually (Iran) Transmitted Diseases in view of reproductive health care providers in Health care centers of Yazd, Iran in 2014 15:20-15:30 Isolation of spermatogonial Stem Cells from Shabani R, Ph.D. Candidate tumoral cells by drug delivery (Iran) 15:30-15:40 3D Sonohysterography for the Investigation of Ahmadi F, M.D. Female Infertility: Technique and Application (Iran) 15:40-15:50 First successful pregnancies following embryo Faramarzi A, Ph.D. Candidate selection using Time Lapse technology in Iran: (Iran) Case reports The investigation of transcript expression level 15:50-16:00 of mitochondrial transcription factor A Allahveisi A, Ph.D. Candidate (TFAM) in single human oocytes during oocyte (Iran) maturation 16:20-16:45 Closing session st 1 Reproductive Genetics Congress 18-19 April, 2015 Shahid Sadoughi Hospital, Hall B 18 April 2015, Saturday Panel I Panel Coordinators: Sheikhha MH, M.D., Ph.D., Karimzadeh Meybodi MA, M.D. 11:10-13:00 Genetics & Female Infertility Part (1): Ovulation problem Panel Members: Afsharian P, Ph.D. Mazaheri M, M.D., Ph.D. Nouri Daloui M, Ph.D. Salehi M, Ph.D. Shahhoseini M, Ph.D. Panel II Panel Coordinators: Dehghani Firouzabadi R, M.D., Ghasemi N, M.D., Ph.D. 14:00-15:30 Genetics & Female Infertility Part (2): Implantation failure & RPL Panel Members: Eftekhar M, M.D. Farashahi yazd E, Ph.D. Sanaati MH, Ph.D. Shekari M, M.D., Ph.D. 19 April 2015, Sunday Panel III Panel Coordinators: Kalantar SM, Ph.D., Vafaei H, M.D. 11:10-13:00 Genetics & pregnancy management: or PND and PGD Panel Members: Dalili M, M.D. Gourabi H, Ph.D. Kariminejad MH, M.D. Kariminejad R, M.D. Tabatabai RS, M.D. Zeinali S, Ph.D. Panel IV Panel Coordinators: Dehghani MR, M.D.,Ph.D., Vahidi S, M.D. 14:00-15:30 Genetics & male infertility Panel Members: Hosseini SJ, M.D. Modarsi M, M.D., Ph.D. Moein MR, M.D. Najmabadi H, Ph.D. Omrani M.D. Ph.D. Sedighi Gilani MA, M.D. Zieaie AH, M.D.