SI Worksheet #5 (chapter 10 and 12) BY123 Meeting: 6/24/15 @ 1pm in EB 132 1. Compare and Contrast Cell Respiration and Photosynthesis Compare – Both have an ETC Have ATP synthesized Involve electron carriers Involve multiple memebranes and H+ gradients In organelles with multiple compartments Both have mitochondria ContrastPhoto Creates and fixes energy (Endergonic) (Exergonic) Chloroplast Makes NADpH 2. Cell Rep requires and releases energy Makes NADH True or false: Plants are the only organisms that perform photosynthesis. False. Lots of organisms including different bacteria, protists, and alga perform photosynthesis. 3. Draw out the balanced equation for photosynthesis. What type of a reaction is this? 6 CO2 + 12 H2O + Light energy → C6H12O6 + 6 O2 + 6 H2O This is an endergonic reaction. It requires light energy to be put into the reaction in order for it to proceed 4. Photosynthesis is a redox reaction. What is oxidized, and what is reduced? CO2 is reduced. H2O is oxidized. 5. What are the two parts of photosynthesis? Where do they occur? What happens in each? The two parts of photosynthesis are the light reactions and the Calvin Cycle. The light reactions occur in the thylakoid membrane and require light in order to proceed. In the light reactions H2O splits and O2 is produced, NADPH is produced, and ATP is produced. The Calvin Cycle is light independent and occurs in the stroma. The Calvin Cycle relies on the ATP and NADPH that were produced in the light reactions and is the part in which sugar is synthesized. 6. What in chloroplasts absorbs light? Color pigments absorb light. Chloroplasts have chlorophyll a, chlorophyll b, and accessory pigments called the carotenoids. These pigments will absorb certain ranges of light in the visible light spectra. The color that they appear to be is actually the color that they reflect not what they absorb (chlorophyll actually does not absorb green light). Pigments absorb photons, which causes electrons to become excited and heat and the photon is released as the electron falls back down to the ground state. 7. How is ATP synthesized in the light reactions? Describe this part of the process. ATP is synthesized using photophosphorylation, which is very similar to oxidative phosphorylation in cellular respiration. The electron transport chain that connects PSII and PSI is embedded in the thylakoid membrane. It causes the buildup of protons in the thylakoid space, making the thylakoid space have a higher proton concentration than the stroma, building a proton motive force. Protons then move through ATP synthase which is also embedded in the thylakoid membrane, and causes the formation of ATP in the stroma. 8. What is produced by each run of the light reactions? What is produced by each run of the Calvin cycle? The linear electron flow light reactions produce 1 ATP and 1 NADH and require 1 water molecule and sunlight. The cyclic electron flow light reactions produce 1 ATP and require sunlight. The Calvin cycle requires 3 CO2, 9 ATP, and 6 NADPH and produces 1 G3P. 9. How many pairs of chromosomes does a human have? How many pairs are autosomes? How many are sex chromosomes? What combination of sex chromosomes constitute male and female? 23… 22…. 1 ….. XX female…. XY male… 10. Describe the DNA of a bacteria… how does it divide? Single circular chromosome that does binary fission which is formed by the replication of the single chromosome, the separation of the chromosome copies, and finally the pinching of the cytoplasm which results in 2 identical daughter cells. 11. What are the 3 reasons to do mitiosis? 1. Mitosis as a form of reproduction 2. Growth and development 3. Tissue Renewal 12. What are the two types of cells in multicellular organisms? What are the processes by which these cells are replicated? The two types of cells in multicellular organisms are somatic (body) cells and gamete (sex) cells. Somatic cells are created through mitosis. Gametes are created through meiosis. 13. Define sister chromatids. What are they held together by? Sister chromatids are identical copies of the same genetic material that are held by a centromere. 14. What are the 3 parts of interphase? 1. G1 2. S – Synthesizes DNA, copies itself. 3. G2 – cant see anything visible, but the cell has already replicated its DNA. 15. What are the 4 main parts of Mitosis? Describe each. a. Prophase- Start to see chromosomes (centromeres connecting sister chromatids), nuclear envelope breaks, nucleolus goes away, One centriole moves toward the pole while the other stays in its original position. b. Metaphase – Chromosome pairs line up at the equator of the cell and make the metaphase plate, there are 2 types of spindle fibers- 1. Kinetochore- These connect directly to chromosomes by way of the kinetochore protein. 2. Non kinetochore proteins – run pole to pole don’t attach to the chromosomes. 46 chromosomes are lined up at the metaphase plate… these 46 chromosomes contain 92 sister chromatids. c. Anaphase – The sister chromatids are pulled apart by way of the shortening of the attached kinetochore proteins. The non-kinetochore proteins lengthen causing the entire cell to elongate. Important motor molecule of microtubules is DYENIN. d. Telophase – the nuclear envelope comes back and also the nucleolus… Cytokinesis or the formation of a cleavage furrow begins which is facilitated by microfilaments and the cell begins to split into 2 cells each with the original amount of genetic material. 16. Describe the G1, M, G0 and G2 checkpoints. G1- The checkpoint is crossed when growth factors tell the cell that it needs to begin copying its DNA G2 – After all the genetic material has been copied the cell passes G2 which tells the cell everything is good to go to begin mitosis M – Tells the cell it is ready to finish mitosis. G0 – these cells are also called post-mitotic because they do not have the ability to do mitosis. Muscle and brain cells are in this category. These cells literally come Out of the cell cycle. 17. What do diploid and haploid mean? Diploid cells are referred to as 2n and have a two of each chromosome (somatic). Haploid cells are referred to as n and have only one of each chromosome (gametes). 18. What is cancer? How does it develop? General term for disease caused by the uncontrolled division of cells that spread to other areas of the body (metastasis). All cancers involve the failure of checkpoints 19. Describe meiosis. Tell how it is similar and how it differs from mitosis. What are the two different stages called? How many cells are produced? Before meiosis the DNA is replicated. Meiosis follows the same general steps as mitosis with slightly different things happening at each step. Prophase I: homologous chromosomes form a synapsis. Crossing over occurs in which the chromosomes swap DNA for some sections of the chromosome forming a chiasma. The rest is like mitosis. Metaphase I: Homologous pairs line up on the metaphase plate. Anaphase I: The homologous pairs are pulled apart. Sister chromatid are still intact! Telophase I: The division is completed and the cells are now haploid. Meiosis II is like mitosis and works to separate the sister chromatids. Meiosis I: reductional division Meiosis II: equatorial division Four daughter cells are produced.