Chang Dou
Biofuels and Bioproducts Laboratory
School of Environmental and Forest Sciences
College of Environment
University of Washington
E-mail: douchang2011 <at> gmail dot com
Phone: (206) 543-9271
http://changdou.weebly.com/
Education
University of Washington, Seattle
PhD of Environmental and Forest Sciences (Expected Graduation: 2016)
Master of Bioresource Science and Engineering (2013) GPA (3.7/4.0)
School of Environmental and Forest Sciences (Advisor: Dr. Renata Bura)
Nanjing University of Technology (NJUT), Nanjing, China 2011
Bachelor of Science (Biochemical Engineering Direction) GPA (3.8/4.0)
College of Biotechnology and Pharmaceutical Engineering (Advisor: Dr. He Huang)
Research Experiences
Graduate Research Assistant (Sep. 2011 – Now)
Biofuels and Bioproducts Laboratory, University of Washington
 Master thesis: Improving carbohydrate recovery from uncatalyzed steam pretreated hybrid poplar.
 Bioconversion of lignocellulosic material to bioethanol. Worked with many types of biomass, from wood
waste to agricultural residues (wheat straw, sugarcane bagasse) to energy crops (Hybird Poplar, Giant
reed, Arundo donax).
 Optimizing conditions in pretreatment, enzymatic hydrolysis and fermentation to get a higher yield of
fuels and chemicals from lignocellulosic biomass.
 Investigated the relationship between different steam explosion conditions and inhibitor accumulation.
And studied the influence of inhibitor in xylitol and ethanol yields in fermentation.
 Presented in 10th SEFS Annual Graduate Student Symposium. March 8 2013.
 Presented in 35th Symposium on Biotechnology for Fuels and Chemicals. Portland, OR (May. 1)
Bioresource Pilot Laboratory, University of Washington
 Introduced refining, a mechanical process after steam explosion, to promote the enzymatic hydrolysis
efficiency by homogenizing the particle size and increasing the fiber surface area.
 Presented a poster in 35th Symposium on Biotechnology for Fuels and Chemicals. Portland, OR (May. 1)
 Attended WPPF Annual Conference. Seattle, WA (May.24)
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Attended Northwest Bioenergy Research Symposium. Seattle, WA (Nov.13)
Research Assistant (Jan. 2011- June 2011)
Laboratory of Industrial Catalysis and Metabolic Engineering, NJUT
 Bachelor thesis: The expression of BL21-pET22b-FumR in recombinant E. coli and the study of fumarase
enzymatic property.
 Recombined the BL21-pET22b-FumR gene into E.coli and analyzed the enzymatic property of fumarase.
 Participated in writing a proposal of National Science Foundation of China (NSFC) which concerned about
the lipase production from Bacillus subtilis at the level of gene & enzyme, including gene site-directed
mutation & heterologous expression, and enzyme isolation & purification.
Research Assistant (Feb. 2010-Dec. 2010)
National Engineering Research Center for Biotechnology
 Participated in a project of two-stage sugar utilization of corn straw by Rhizopus oryzae for fumaric acid
production, which was sponsored by 973 Program (the National Basic Research Program of China).
 Investigated the metabolic mechanisms of Rhizopus oryzae with different carbon sources, mainly studied
in metabolic differences (NADH/NAD+) in Rhizopus oryaze under xylose and glucose fermentation.
 Presented in the 6th Chinese National Chemical and Biochemical Engineering Meeting, and was the only
undergraduate in oral presentation section. Changsha, China. (Oct.29~31)
 Attended the 5th National Conference of Chinese Society of Biotechnology. Nanjing, China. (Dec.28)
Group Leader (Aug. 2009-Jan. 2010)
State Key Laboratory of Materials-Oriented Chemical Engineering
 As the team leader of a 10-member International Genetic Engineering Machine (iGEM) group, led the
team series of case study and brain-storm.
 Made a presentation: “A specific introduction about iGEM: targets, means, developments and prospects”
to the faculty and supervisors.
 Participated in constructing a blueprint in combination of iGEM with LICME (Laboratory of Industrial
Catalysis & Metabolic Engineering) development.
 Investigated the current progress in fermentative production of expolysaccharides and potential usage of
expolysaccharides from Klebsiella sp., and wrote a review paper about it.
Project Developer (Sept. 2008-Aug. 2009)
SINOPEC Nanjing Joint Research Center for Industrial Biotechnology
 Participated in screening of oil-rich microalgae and developing high efficient CO2 fixation, mainly in the
section of culture optimization and light reactor design.
 Took part in the translation of the “Handbook of Microalgae Culture” into Chinese.
Research Assistant (July 2008 -Sept. 2008)
National Engineering Research Center for Biotechnology
 Participated in the research on high density fermentation of recombinant Pichia pastoris by methanol
regulation for Single Cell Protein (SCP) production.
 Worked on of SCP production in a 50L pilot scale fermenter.
 Participated in the research of selecting dehydration of bio-ethanol to ethylene, especially focused on the
influence of fusel during the catalyzing.
Teaching Experiences
Teaching Helper (Sep. 2011 – Now)
Biofuels and Bioproducts Laboratory, University of Washington
 Instruction of undergraduate researchers.
 Technique support in the BSE 426 Bioresource laboratory class.
Leadership
Minster of Organization (Oct. 2008 – June 2011)
Elite School of NJUT for Scientific Scholar
 One of 60 leaders selected from the entire university.
 Took special courses including Leadership Cultivation, Philosophy, Principles of Management,
International Relations, Jurisprudence, Economics, Psychology, Art, Oral English, etc.
 Organized and participated several activities such as field survival training, governmental practice in rural
areas, high-tech enterprise internship, etc.
President (Sept. 2008-June 2009)
Ocean Eagle League (a Peace Promotion Association) in NJUT
 Organized several multi-school activities, including a military maneuver which helped the group win the
honor of Provincial Outstanding Community.
 Joined the 70th Memorial Day of Nanking Massacre as one of university student committee.
Scholarship & Awards & Honors
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School of Environmental and Forest Sciences Fellowship, 2011-2012
Distinguished Graduates of NJUT (top 5% graduates each year), 2011
Outstanding Student Leader, NJUT, 2010
Merchant Bank Scholarship (46 winners among 35,000 students), 2010
Undergraduate Academic Forum, Grand Prize (5 winners among 30,000 undergraduates), 2010. Third
Prize, 2009
NJUT Scholarship, 2009, 2008
National Biology Olympiad Competition, Second Prize, 2006
Publications & Abstracts
Chang Dou, Shannon Ewanick, Renata Bura. Post steam explosion refining to increase cellulose conversion during
enzymatic hydrolysis [J] Biotechnology and Bioengineering. (Working paper)
Azra Vajzovic, Chang Dou, Shannon Ewanick, Neethi Nagarajan, Renata Bura. Enhanced xylitol and ethanol yields
by fermentation inhibitors in steam pretreated hydrolysates [J] J Ind Microbiol Biotechnol (Working paper)
Chang Dou, Jun Du, Xiaojun Ji, Zhikui Nie, Zheng Gao, He Huang. Research progress in the fermentative production
of expolysaccharides by Klebsiella sp. [J] China Biotechnology 2010, 30(12) 123-127. (In Chinese with English
abstract)
Chang Dou, Qing Xu, Ling Jiang, Shuang Li. The metabolism research of Rhizopus oryzae with xylose and glucose as
carbon resource [J] Acta Microbiologica Sinica. 2011, 51(4):468-473. (In Chinese with English abstract)
Yueyue Ding, Shuang Li, Chang Dou, Yang Yu and He Huang. Production of fumaric acid by Rhizopus oryzae: role of
carbon-nitrogen ratio. [J] Applied Biochemistry and Biotechnology 2011, 164(8): 1461-1467.
Yaochi Hu, Nina Zhan, Chang Dou, He Huang, Yuwang Han, Dinghua Yu and Yi Hu. Selective dehydration of bioethanol to ethylene catalyzed by lanthanum-phosphorous-modified HZSM-5: influence of the fusel. [J]
Biotechnology Journal 2010, 5(10): 1186-1191.
Hongli Zheng, Jilong Yin, Zhen Gao, He Huang, Xiaojun Ji and Chang Dou. Disruption of Chlorella vulgaris cells for
the release of biodiesel-producing lipids: a comparison of grinding, ultrasonication, bead milling, enzymatic lysis,
and microwaves. [J] Applied Biochemistry and Biotechnology. 2011, 164(7):1215-1224.
Hongli Zheng, Zhen Gao, Qi Zhang, He Huang, Xiaojun Ji, Honglei Sun, Chang Dou. Effect of inorganic carbon source
on lipid production with autotrophic Chlorella vulgaris. [J] Chinese Journal of Biotechnology 2011, 27(3):436-444
Chang Dou, Qing Xu, Shuang Li. Relationship between Coenzyme I and Organic Acid Production under Cultivation
of Xylose [C] 6th Chinese National Chemical and Biochemical Engineering Annual Meeting. Chang Sha, China
2010(10) Oral
Chang Dou, Shannon Ewanick, Rick Gustafson, Renata Bura. Post steam explosion refining to increase cellulose
conversion during enzymatic hydrolysis [C] 35th Symposium on Biotechnology for Fuels and Chemicals. Portland, OR
2013 (5) Poster
Laboratory Skills
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Engineering software operations: Matlab, Aspen, Visual Basic, etc.
Analytical Chemistry: High Performance Liquid Chromatography (HPLC), FT-IR Spectrometer, Gas
Chromatography (GC), Raman Spectrum, Klason test.
Bimolecular techniques: SDS-PAGE, PCR, Bicinchoninic Acid Assay (BCA), Fluorescence Microscope.
Microbiology techniques: Isolation and Identification of Strains, Fermentation on Shake Flask Level,
Operation of NBS Bioreactor, Operation of Industrial Level Fermenters from 50L to 25tons, etc.
Computer Application Skills: Skilled in Word, Excel, PowerPoint, ect.
Skilled in statistical analysis & tools: SPSS, R, Origin, etc.