Table S1.
Group
Range of assayed
temperatures (ºC)
Range of pKa
Range of Cw
(M)
50-100
6.0-6.1
4867-5970
Chromatium tepidum
5-70
6.0-6.3
202-440
Chromatium vinosum
Thiobacillus sp. (endosymbiont of
Alviniconcha hessleri)
Thiobacillus thyasiris
5-60
6.0-6.3
211-440
2-70
6.0-6.5
889-2383
5-77
6.0-6.3
207-385
Cyanidium caldarium
20-70
6.1-6.2
202-292
Anabaena variabilis
15-40
6.1-6.2
44-76
Synechococcus lividus
20-70
6.1-6.3
62-90
Synechococcus strain a-1
25-75
6.0-6.1
1572-2174
Chlamydomonas reinhardtii
0-70
6.1-6.4
115-252
Chlorella emersonii
2-60
6.1-6.5
211-576
Chloromonas strain ANT1
0-70
6.1-6.4
115-252
Chloromonas strain ANT3
0-70
6.1-6.4
115-252
Alopecurus alpinus
0-60
6.1-6.5
978-2320
Arctagrostis latifolia
0-60
6.1-6.5
978-2320
Atriplex glabriuscula
5-35
6.1-6.3
78-192
Chenopodium album
16-40
6.1-6.2
110-144
Dupontia fisheri
0-60
6.1-6.5
978-2320
Espeletia schultzii
5-35
6.1-6.3
80-116
Pinus sylvestris
15-60
6.0-6-2
327-492
Poa arctica
16-40
6.1-6.2
110-144
Poa pratensis
16-40
6.1-6.2
110-144
Solanum tuberosum
16-40
6.1-6.2
110-144
Spinacia oleracea
5-33
6.1-6.3
86-123
Triticum aestivum
17-30
6.1-6.2
185-189
Agropyron smithii
10-40
6.1-6.3
400-460
Arachis hypogaea
16-40
6.1-6.2
110-144
Astragalus flavus
5-72
6.1-6.4
225-487
Astragalus rafaelensis
5-72
6.1-6.4
225-487
Capsicum chinense
16-40
6.1-6.2
110-144
Flaveria pringlei
16-40
6.1-6.2
110-144
Glycine max
15-65
6.1-6.3
251-387
Gossypium hirsutum
16-40
6.1-6.2
110-144
Nicotiana tabacum
25-50
6.1-6.2
372-456
Oryza sativa
16-40
6.1-6.2
110-144
Pueraria lobata
16-40
6.1-6.2
110-144
Solanum lycopersicum
5-72
6.1-6.4
225-487
Stanleya pinnata
5-72
6.1-6.4
225-487
Trifolium repens
10-25
6.1-6.3
164-214
Amaranthus retroflexus
16-40
6.1-6.2
110-144
Andropogon gerardii
0-60
6.1-6.5
978-2320
Species
Archaea
Thermococcus kodakariensis
Proteobacteria
Rhodophyta
Cyanobacteria
Chlorophyta (green algae)
Spermatophyta (C3 plants from cool habitats)
Spermatophyta (C3 plants from warm habitats)
Spermatophyta (C4 plants)
1
Bouteloua gracilis
1-37
6.1-6.5
79-196
Cynodon dactylon
16-40
6.1-6.2
110-144
Digitaria sanguinalis
16-40
6.1-6.2
110-144
Flaveria trinervia
16-40
6.1-6.2
110-144
Muhlenbergia montana
16-40
6.1-6.2
110-144
Portulaca oleracea
16-40
6.1-6.2
110-144
Sorghum bicolor
0-60
6.1-6.5
978-2320
Zoysia japonica
16-40
6.1-6.2
110-144
The ranges of temperatures available, and corresponding acidity constants of dissolved
carbon dioxide (pKa) at different temperatures and the liquid phase concentrations of the
dissolved CO2 in the assay media for each of the species considered in the present
compilation. pKa was calculated based on the assay medium ionic strength and
temperature according to Yokota and Kitaoka (1985), and these values were employed
to estimate the equilibrium CO2 concentration from added bicarbonate concentration.
Yokota A. Kitaoka S. (1985) Correct pK values for dissociation constant of carbonic
acid lower the reported Km values of ribulose bisphosphate carboxylase to half.
Presentation of a nomograph and an equation for determining the pK values.
Biochem Bioph Res Co 131:1075-1079.
2
Figure 1S. Values of Rubisco maximum carboxylase turnover rate (kcatc) normalized to
25ºC at a range of temperatures among (A) all phylogenetic groups, and (B) among land
plants groups. Values for kcatc were obtained at discrete temperatures after applying
equation 1 (see Methods); means and standard errors are presented Symbols of the
phylogenetic groups in (A): empty downward triangles and dotted line, Archaea (n = 1);
empty upward triangles and short-dashed line, Proteobacteria (n = 4); empty circles and
dash-dotted line, Cyanobacteria (n = 3); empty squares and dash-dotted-dotted line,
Chlorophyta – green algae (n = 4); diamond and long-dashed line, Rhodophyta – red
algae (n = 1); filled square and solid line, Streptophyta – land plants (n = 36). Symbols
of the groups in (B): solid downward triangles and dotted line, C3 cool (n = 12); solid
upward triangle and long-dashed line, C3 warm (n = 14); solid circle and line, C4 (n =
10).
3
Ln kcat normalized to 25ºC
c
c
Ln kcat normalized to 25ºC
10
6
A
8
6
4
2
0
B
5
4
3
2
1
0
5
15
25
35
45
Temperature(ºC)
4
Figure S2. Phylogenies of (A) all taxa and (B) Spermatophyta – land plants only, that
were used in phylogenetic independent contrast analyses. The phylogeny (A) is a
composite phylogeny based on 16S ribosomal RNA and rbcL sequences, and all branch
lengths were assigned to one. The rbcL-based phylogeny (B) was reconstructed using a
maximum-likelihood inference conducted with RAxML version 7.2.6 (Stamatakis
2006).
5