Brandon Gadberry Animal Science Bio Test 2 Material: Unit 8, Unit 9, Lab Notes 5A & 5B and online animal bone homework Unit 8 Animal Nutrition: Digestion Digestion- breakdown of complex molecules present in feedstuffs into their smaller, individual components. o Digest starchindividual molecules of glucose Absorption- transport of these small molecules across the wall of the digestive tract into the bloodstream Carnivores- primarily meat (proteins, fats, bone) o Easy diet for animals to digest o Short and simple digestive tract o Dogs and cats Omnivores- significant amounts of both plants and animals in their diet. o Plant- seeds and fruits, rich in starch, sugars, and oils. o Easily digested; relatively simple digestive tracts o Pigs and humans Herbivores- nutrients from plant food products o Low-fiber herbivores; consume primarily seeds and fruits Can digest starches, sugars, and oils quite readily o High-fiber herbivores; consume primarily leaves and stems Consume plants rich in cellulose and other fiber Animals lack enzymes needed to break down the beta-1,4 glycosidic bond that links glucose molecules together in cellulose. Depend on microorganisms Animals that have this have digestive tracts with large capacities Most of our commercially important livestock species are high fiber herbivores. Digestive Process- breakdown of complex feedstuffs into absorbable nutrients Mechanical Process o Mastication- chewing Reduces the particle size of feedstuffs, making it easier for them to move down the digestive tract and increasing the surface area that is exposed to other digestive processes. o Mixing- keeps food material exposed to the other digestive process Parts of the rumen are constantly undergoing contractions to aid mixing o Peristalsis- rhythmic, coordinated contraction of the gut that is the primary means of moving food from one part of the digestive tract to the next. Chemical Process o Enzymatic and nonenzymatic Enzymes are protein molecules that catalyze biochemical reactions Digestive enzymes are enzymes that catalyze the breakdown of feedstuffs in the digestive tract. Pancreatic amylase secreted from pancreas and breaks starch down into the disaccharide maltose Non-enzymatic; stomach secretes hydrochloric acid to assist protein digestion Mechanical Process o How cellulose and other fibrous materials are broken down Anatomy of the Digestive Tract: MONOGASTRIC ANIMALS Monogastric animals; simple stomached animals PIG Types of digestion Starch Protein Fat Cellulose Mouth; Saliva (contains buffers that neutralize acids) Mechanical (mastication) NONE NONE NONE Esophagus NONE NONE NONE Stomach Mechanical (peristalsis) Chemical (protein) Ptyalin begins breaking down alpha-1,4 glycosidic bonds NONE NONE NONE Small Intestine; duodenum, jejunum, ileum -Bile (synthesized in the liver and stored in the gall bladder) -Pancreatic lipase breaks the bond between glycerol and fatty acids NONE Chemical (starch, fat, protein) NONE -Microbial digestion Large Intestine; Cecum and colon Microbial NONE -Pepsin and HCl cleave specific peptide bonds -Pancreatic -Pancreatic amylase breaks enzymes(trypsin, starch into chymotrypsin, maltose and -Intestinal carbopeptidase) maltase breaks cleave specific down maltose peptide bonds into glucose which can be Aminopeptidases absorbed and dipeptidases -Glucose is break down transported into peptides into the intestinal individual amino cells by acids specific -Individual glucose amino acids are transport absorbed by proteins specific amino acid transport proteins NONE NONE break down beta-1,4 glycosidic bonds How does digestion differ for horses? o Mouth: Same as pig o Stomach: same as pig o Small Intestine: same as pig o Large Intestine: Increased size and complexity Slow transit time Better utilization of Volatile Fatty Acids released by microbes How does digestion differ for chickens? o Mouth: No saliva No mastication o Esophagus Food storage in crop o Stomach (2 parts) Proventriculus (Chemical Digestion) Gizzard (reduce particle size) o Small Intestine: Same as pig and horse o Large Intestine 2 ceca, otherwise similar to pigs Ruminant Primary types of digestion o Mouth o Esophagus o Stomach Rumen Reticulum Omasum Abomasum o Small Intestine o Large Intestine Cellulose Protein Mouth Types of Starch digestion Mechanical NONE NONE NONE Esophagus Mechanical NONE NONE NONE Rumen Microbial Microbes break beta-1,4 glycosidic bonds - Use glucose for own metabolism and release Volatile - Microbes use the protein for themselves and convert it to microbial protein Ruminant Microbes break beta-1,4 glycosidic bonds - Use glucose for own metabolism and release Volatile Fatty Acids -Volatile Fatty acids are absorbed by the animal Reticulum Microbial NONE Fatty Acids -Volatile Fatty acids are absorbed by the animal NONE Omasum Microbial NONE NONE NONE Abomasum Chemical NONE NONE Small Intestine Chemical NONE NONE -Lysozyme breaks down bacterial cell walls -HCl and pepsin cleave peptide bonds -pancreatic enzymes(trypsin, chymotrypsin, and carboxypeptidase) cleave specific peptide bonds -Amino peptidases and dipeptidases break peptides into individual amino acids -Individual amino acids are absorbed by specific amino acid transport proteins Large Intestine Microbial NONE -Microbes break down beta-1,4 glycosidic bonds and use the resulting free glucose for their own metabolism NONE NONE Unit 8 Questions 1. What are the three major groups that animals can be classified in based on their principal source of nutrients? o Omnivore, herbivore, and carnivore. 2. Distinguish between low-fiber and high fiber herbivores. o Low fiber herbivores: consume primarily seeds and fruit. Can digest starches, sugars, and oils. o High fiber herbivores: consume primarily leaves and stems. Consume parts of the plant that are rich in cellulose and other fiber. 3. What are the three major digestive processes and give examples of each? o Mechanical: Mastication in the mouth, Mixing, and Peristalsis o Chemical: Enzymatic and Non-enzymatic; stomach secretes hydrochloric acid to assist protein digestion. o Microbial: cellulose is broke down 4. Identify each of the major parts of the digestive tract from a Monogastric animal. o Mouth: mechanical digestion (mastication) o Esophagus: Mechanical digestion (peristalsis) o Stomach: chemical digestion (protein) o Small Intestine: chemical digestion (starch, fat, and protein) o Large Intestine: microbial digestion 5. Identify the products secreted by each of these parts and how they contribute to digestion. o Mouth: Saliva- helps soften the food and serves as a lubricant for its passage further in the digestive tract. Contains buffers that neutralize acids. Also contains ptyalin that breaks down alpha-1,4 glycosidic bonds. o Esophagus: Nothing o Stomach: Hydrochloric acid- breakdown the peptide bonds Pepsin-proteolytic enzyme; cleaves peptide bonds between specific pairs of amino acids o Small Intestine: Bile- bile is synthesized in the liver and stored in the gall bladder; released in the duodenum; it breaks up fat into smaller particles to mix more efficiently. This is an important for of nonenzymatic, chemical digestion. Pancreatic lipase- breaks the ester bonds that link fatty acids to glycerol in triglycerides and phospholipids. Pancreatic amylase- breaks down starch into maltose o Large Intestine Cellulolytic enzymes- cleave the beta-1,4 glycosidic bonds that link glucose to molecules in cellulose. 6. What is a proteolytic enzyme? Give some examples. o A group of enzymes that break the long chainlike molecules of proteins into shorter fragments (peptides) and eventually into their, components amino acids. An example is pepsin. 7. Describe how starch, cellulose, triglycerides, and proteins are digested in each part of the digestive tract. o SEE CHART ABOVE FOR MONOGASTRIC 8. Describe where and how nutrients are absorbed into the bloodstream. o Nutrients are absorbed in the bloodstream in the large intestine and since volatile fatty acids are very soluble the can easily cross the large intestine wall and into the bloodstream. 9. Be able to describe where digestion begins, continues, and is completed for starch, cellulose, triglycerides, and protein. o Starch: begins in mouth and ends in small intestine o Cellulose: begins and ends in large intestine o Triglycerides: begins and ends in small intestine o Protein: begins and ends in small intestine 10. In addition to digestion, describe the functions of the large intestine. o The large intestine can be use to provide Volatile Fatty Acids as a source of energy as well as the microbes synthesizing vitamins. The large intestine is also a major site for water reabsorption. 11. Describe how the digestive system of the horse has been adapted to a high-fiber diet. o It has adapted to a high-fiber diet in a simple manner. First, its teeth are designed to harvest and chew fibrous feedstuffs. The incisors are designed to shear grass stems and check teeth are hypsodont with parallel ridges of enamel that are very effective for chewing/grinding surface. Second, horses have retained the basic elements of the Monogastric tract except they have greatly increased the capacity for fiber digestion by expanding the cecum and colon. 12. Describe how each of these parts in the digestive tract of poultry differs from the corresponding part in Monogastric mammals: mouth, esophagus, stomach, cecum. o Mouth: No saliva and no mastication o Esophagus: Food storage in the crop o Stomach: Proventriculus (chemical digestion) and Gizzard (reduce particle size) o Cecum: 2 ceca instead of one 13. Name the four compartments of the ruminant stomach. o Rumen, Reticulum, Omasum, Abomasum 14. Describe how each compartment functions in digestion o Rumen: microbial digestion- ruminate or regurgitate food to maximize efficiency of microbial digestion o Reticulum: microbial digestion- Filter; inside of the reticulum is honeycomb shaped ridges and catch larger particles of leaves and stems and push them back into the rumen o Omasum: microbial- secondary filter; numerous sheets of tissue project from the walls of the omasum allowing only the finest particles to pass through o Abomasum: microbial- true glandular stomach; HCl and pepsin are added here, lysozymes are secreted to break down cell walls. Digestion of microbes begin in abomasum 15. Describe how microbial digestion provides energy to the ruminant. o 16. Describe how microbial digestion contributes protein to the ruminant. o 17. What are the advantages of a ruminant compared to a Monogastric herbivore like a horse? o 18. Under what conditions is a horse more efficient than a ruminant? 19. Describe how the rumen changes as the animal matures. o At birth the rumen is relatively small making up only 25% of the stomach capacity. At adulthood the rumen has enlarged to make up 80% of the total stomach volume. 20. How do the relative sizes of each portion of the digestive tract reflect the type of diet and the importance’s of microbial verse chemical digestion in the pig, horse, and cow? o Pig: the pig has relatively the same sizes throughout the digestive tract. This shows that the pig is and omnivore because it consumes both plants and animals. This is important for chemical digestion to secrete HCl and pepsin in the stomach to break down the foods. Fore gut fermenter o Horse: in the horse the large intestine is significantly bigger because it is a hind gut fermenter. This means the horse has greatly increased the capacity for fiber digestion in the cecum and colon. Grain is digested in the upper digestive tract (mouth, stomach, and small intestine). “tough stuff” such as cellulose and fiber isn’t digested until the large intestine. o Cow: the rumen, reticulum, omasum, and abomasum are the biggest parts of the cow. This shows that microbial digestion is done here and where most of the digestion is done then it is absorbed in the large intestine. UNIT 9 1) What is meant by the terms metabolism, catabolism, and anabolism o Metabolism: the study of all of the biochemical reactions that is required to support normal body functions. It includes the regulation 2) 3) 4) 5) 6) 7) 8) of those reactions by the level of substrates, metabolites, and hormones. o Catabolism: the breakdown of complex molecules into smaller ones. Catabolic reactions generate energy o Anabolism: build up complex molecules form less complex precursors. Anabolic reactions consume energy Define growth o Growth is an increase in body mass. Growth is anabolic. When anabolism exceeds catabolism the body grows. Growth requires energy. What is meant by the terms hypertrophy and hyperplasia? How do they differ? o Hypertrophy: cells increase in size o Hyperplasia cells can divide and increase in number Describe the three types of cells: ectoderm, endoderm, and mesoderm. o Ectoderm: forms the skin and nervous system o Endoderm: forms the digestive tract, liver, and lungs o Mesoderm: differentiate into stem cells that form three broad types of tissue, the urinary and reproductive systems, the circulatory system, and the structural components of the body. Describe these three types of cells: adipocytes, myoblasts, and osteoblasts. o Adipocytes: Fat cells o Myoblasts: form muscle cells o Osteoblasts: form bone How are gross, digestible, metabolizable, and net energy different? Which is better? o Gross Energy: the total amount of energy available in feed. It is determined by igniting the feed and combusting it completely in an atmosphere of pure oxygen. o Digestible Energy: subtract fecal energy from gross energy then you have what the animal digested. This is a better estimate of what the animal will really get from the feed than the gross energy. o Metabolizable Energy: subtract the amount of energy lost in the urine and in gas, we are left with metabolizable energy. Energy is loss during these reactions in the form of heat. o Net Energy: if you subtract the heat loss you get net energy. This is the most precise measurement of energy content of a feed. What is maintenance as it persists to energy metabolism o Maintenance is breathing, pumping blood, acquisition of food, digestion, escape, etc. o Therefore the lungs, heart, critical muscle systems, and the brain have high priority for glucose utilization. What does the terms positive and negative energy balance mean? o Positive energy balance: if the glucose coming into the blood exceeds the maintenance requirement, then the animal is said to be in positive energy balance. The animal can devote the excess glucose to production. This includes growth, reproduction, and lactation. o Negative energy balance: when animals don’t have enough glucose to meet their maintenance requirement. They then rely on stored energy in the body to put glucose back in the bloodstream. 9) Describe how glucose is stored during positive energy balance. o If there is still glucose in excess of the production it can be stored. It can be stored in the muscles, liver, and fat. In fat glucose is taken up by adipocytes and used by them to synthesize fatty acids and glycerol. They assemble these into triglycerides for storage. 10) Describe how glucose levels are maintained during negative energy balance o Glycogen is the first storage form to be used. The cells that store glycogen break down into glucose. They will use it for energy and release the excess in the bloodstream. If negative energy balance persists more than a day then the animal will turn to the energy stored in fat. Lipase enzymes in the adipocytes are activated to cleave triglycerides into free fatty acids and glycerol. Then they are released into the bloodstream, taken up by the liver, and used to synthesize new glucose. If the negative energy persists even further the animal must resort to extreme measures to meet its glucose demand. Muscle cells begin to break down muscle protein into free amino acids and then the amino acids are released into the bloodstream. 11) Describe the embryological development of the tissues that make up the carcass o All cells in the body are derived from undifferentiated stem cells which then differentiate into three types of cells; ectoderm, endoderm, and mesoderm. The structural stem cells will then differentiate into three more types of cells; adipocytes, myoblast, and osteoblasts. Thus the three main components of the carcass are all derived from the same stem cell. 12) Describe how growth grate changes over time. o At birth growth rate isn’t very efficient but at the time of weaning and puberty this is the most efficient the growth rate will ever be. After this time period the animals starts to get less efficient. 13) Describe how the growth rates for particular tissues changes over time. o The central nervous system matures the most quickly reaching its maximum growth rate just prior to birth. The skeletal system is next. Maximum growth rate occurs in the period around weaning. The skeletal system still grows after that but the rate is just slower. Muscle growth is next, peaking around puberty. It is around this time that the body gets close to its mature size. The rate of muscle cells growth declines and the rate of fat cell growth increases. 14) Describe how the efficiency of growth changes with time o Feed to gain ratio; this is the lbs. of feed required to produce 1 lb. of gain. As the animal matures the feed to gain ratio will increase, this is the amount of feed required to produce 1 lb. of gain increases. In other words the animal becomes less efficient with age. 15) What is meant by the term compensatory growth? o Compensatory growth is the catch up growth and compensatory gain is an accelerated growth of the organism following a period of slowed development, particularly as a result of nutrient deprivation. 16) Be able to give a typical birth weight, mature weight, and mature age for each of the major livestock species. Species Birth Weight Mature Weight Mature Age Horses 100-200 900-1100 4-5 Pigs 2-4 500-700 2-3 Sheep 6-12 80-200 2 Goats 6-10 80-220 2 Cattle 60-110 900-1800 4-5 Chickens 2 oz. 7-10 25 weeks Turkeys 2 oz. 15-35 30 weeks 17) Be able to give the proper names for livestock animals based on their age and sexual status CLASSIFICATION OF “MATURE” LIVESTOCK BASED ON SEXUAL STATUS Species Mature Male Castrated Male (prepubertal) Castrated Male Young (postpubertal) Male Mature Female Young Female Horses Pigs Sheep Stallion Boar Ram Gelding Barrow Wether Gelding Stag Stag Mare Sow Ewe Goats Cattle Chickens Turkeys Buck Bull Rooster Tom Filly Gilt Ewe Lamb Doeling Heifer Pullet Steer Capon Stag Ram Lamb Bullock Doe Cow Hen Hen AGE CLASSIFICATIONS OF YOUNG LIVESTOCK Species Newborn Horses Pigs Sheep Goats Cattle Chickens Foal Piglet Lamb Kid Calf Chick Prior to sexual maturity Shoat 1-2 years old Parturition Yearling Yearling Yearling Yearling Yearling Foaling Farrowing Lambing Kidding Calving Turkeys poult How do we know we are feeding an animal enough? o Body condition o Performance/ production- performance, reproduction, and lactation How do we provide energy to animals? o Carbohydrates o Fats o Protein-able to be used but not a good energy source Good sources of energy o Shelled corn o Timothy hay o Vegetable oil o Corn silage