Scientific Program March 15 -20, 2015 www.icmss2015.com Type of Presentation CONFERENCE Presenter Title Climate change: A Latin American Perspective in Mitigation and Adaptation. Genetic considerations for molluscs production in aquaculture: Current State of Knowledge. Growth of Bacteria in Shellfish and the emergence of seafood related outbreaks. Metagenomics for pathogen detection in Shellfish. Marcelo Mena ExDirector Center for Sustainability Research at Universidad Andres Bello Chile & Under Minister of the Environment of Chile Marcela Astorga Universidad Austral de Chile Romilio Espejo. Omics-Chile WORKSHOP Fluorometry of High Repetition FRRF= Applied to photosynthetic cells of dinoflagellates/Fluorometria de altas tasas de repetición FRRF3 aplicado a células fotosintéticas de dinoflagelados Detection kit for Biotoxins. Alejandro Clement & Felipe Perez Laboratorio Plancton Andino. Puerto Varas. Chile David Cassis Jelllet Co. TECHNOLOGICAL TOUR ORIZON SA: Processing plant. MEJILLONES AMERICA SA : Processing plant and shellfish farm. ROUND TABLE International practices in Bivalves sanitation programme Iddya Karunasagar Marisa Caipo Introduction to FAO/WHO work on Technical Guidelines on bivalve. Technical Guideline of Codex Code of Practice for Fish and Fishery Products. Iddya Karunasagar Fish & Fishery Office FAO. Global bivalve production and issues related to international trade Current status of draft Guidelines : Brian Roughan New Zealand Challenges in the implementation of the programme Representative from Asia Representative from Africa Representative from America Comments by Ron Lee CEFAS UK Background and need fortechnical guidance in shellfish sanitation programmer Problems faced by developing countries in implementing shellfish sanitation programmes Namibia: Tobias kuugongel wa Vietnam : Tran Bich Nga Philippines : Sandra Arcamo Morocco : Nada Bougouss REGULATORY ROUND TABLE Iddya Karunasagar FAO EXPERIENCIA CHILENA EN PRODUCCIÓN , CONSUMO Y EXPORTACIÓN Vigilancia de Alimentos Programa de Vigilancia ambiental y seguridad alimentaria en Chile Acuícultura en pequeña escala Experiencia en Codex y Biotoxinas. Silvia Baeza Departamente Nutrición y Alimentos. DIPOL- Minsal.Chile Dra Eugenia Schanke Seremi Salud Región de Los Lagos Claudia Rozas Sernapesca Chile Cecilia Solis Sernapesca Chile La ley de modernización de la inocuidad Alimentaria. Desafíos para las exportaciones de alimentos a los EEUU. Julio Salazar. Subdirector de la FDA para América Latina. Gonzalo Ibáñez. Analista Internacional de la FDA. Oficina Santiago de Chile BIOTOXINS SESSION : Oral presentation Something is happening in fjords and channels of Southern Chile (41° - 55°): the case of Alexandrium catenella and Paralytic Shellfish Poison for the past 40 years. Mussel long-line culture and the risks of toxic Dinophysis in the water column Leonardo Guzmán, IFOP, Chile Brian Roughan, New Zealand Operational Forecasting of Harmful Algal Blooms and Toxicity in Ireland. Joe Silke, Ireland New insights on the ecophysiology of Alexandrium catenella from Chilean fjords. The Red Tide Project as a health monitoring system for the shellfish exposed to Harmful Algal Blooms (HABs) Occurrence of phycotoxins in the coast of Santa Catarina, the largest aquaculture mollusks producer in Brazil. Jorge Mardones, IMAS, University of Tasmania: Mario Castillo, COFEPRIS, México Mathias Schramm, IFSC, Brazil Review of three years of monitoring of palytoxin-group toxins in different marine organisms in Villefranche bay on the French Mediterranean coast. Communicating with stakeholders: new tools to meet challenges The transition from mouse bioassay to chemical methods for the determination of saxitoxin group toxins: the EURLMB experience. Single laboratory validation of a UPLC-HILIC-MS/MS method for quantitation of paralytic shellfish toxins in twelve commercially produced bivalve shellfish species Screening of a wide variety of phycotoxins in different matrices using liquid chromatography high resolution mass spectrometry Volunteer-based harmful algae monitoring networks with rapid toxin detection as an added safety layer and managing option for aquaculture. Mode of action-based bioassays for marine biotoxins causing gastrointestinal disturbances Development and validation of a range of lateral flow immunoassays for the rapid screening of marine biotoxins (ASP, DSP, PSP) in shellfish High throughput assays for sensitive screening of marine neurotoxins in seafood Ronel Biré, ANSES, France Grace Mellano, Canada: Begoña Ben-Gigirey, EURLMB, Spain: Tim Harwood, Cawthron Institute, New Zealand Arjen Gerssen, Rikilt, Holland David Cassis, Jellet, Chile Marcia Bodero, Rikilt, Holland: Karrie Melville, Neogen, UK: Bovee, Toine / Rikilt, Holland: Cell based bioassays for the detection of marine toxins in fish and shellfish for replacing animal testing. Bovee, Toine Rikilt Wageningen, Holland: Temperature effects on kinetics of paralytic shellfish toxin elimination in the Atlantic surfclam Spisula solidissima Allan Cembella, AlfredWegener-Institut, Germany Marine biotoxin assessment of Australian wild-caught abalone. Pinnatoxin-G and other constituents of Vulcanodinium rugosum: analysis, toxicity, distribution in Mediterranean lagoons and accumulation in mussels (Mytilus edulis and Mytilus galloprovincialis Alison Turnbull, South Australian Research and Development Institute, Australia: Philip Hess, IFREMER, France: VIRUS & BACTERIAS ORAL PRESENTATION Impact of NoV infection : An overview including Chilean data. Characterization of outbreaks associated with NoV in Chile. Extraction, Detection and Quantification of Norovirus in Shellfish Associated with Illnesses Investigation of Norovirus Viability. Norovirus detection in oysters: the effect of sample size on the precision of production site virus concentration estimates. Results of three Field Studies presented at the MSC information Meeting: Demostrating Potential Utility of Male-Specific Coliphage in the NSSP. Detección of the virus of Hepatitis A (HAV) in marine organims and other environmental matrices. Evaluation of the characteristics of the wastewater treatment plants for the microbial contamination of bivalve molluscs production areas in the Italian mid-Adriatic coast Miguel O Ryan, Universidad de Chile, Chile Viviana Cachicas, ISP, Chile Jacqueline Wood USFDA/DSST/Dauphin Island, AL David Kingsley, Ag Res Service, Virologist, USDA, USA: Kevin Hunt, Shellfish Microbiology Unit, Marine Institute. Ireland Thomas Howel/ Steve Jones Rodrigo Gonzalez, Universidad de Concepción Chile. Mario Latini, Centro di Referenza Nazionale Ancona, Italy Assessing the impact of risk management procedures on norovirus concentrations in oysters at a production site over a three year period. Occurrence of bacterial and viral enteric pathogens and marine bacteria and discrimination of faecal sources in shellfish-harvesting areas and their catchments in France Bill Dore, Shellfish Safety Marine Institute, Ireland: Michèle Gourmelon, 1LSEM-SG2M-RBE, IIFREMER, Université de Caen, France: Risk Assessment and Risk mapping. Getting the most out of sanitary surveys. Michelle Price Hayward, Cefas, UK: How FDA validated their criteria for sewage treatment plant discharges? Long term of pathogenic VIBRIO SPP. Population in New England U.S. Gregory Goblick. Shellfish and Aquaculture Policy Branch US FDA Stephen H. Jones. Northeast Center for Vibrio Disease and Ecology, University of New Hampshire, USA The Mexican Shellfish Sanitation Program (PMSMB) and the sanitary control of the Vibrio parahaemolyticus. Jose Barreiro, COFEPRIS, México Risk Management of Vibrio parahaemolyticus in Oysters in Canada from 19972014. Enrico Buenaventura, Health Canada, Canada Predictive bases of Vibrio parahaemolyticus in shellfish using Air and Water Temperature for Public Health management and production Vibrio parahaemolyticus and fecal pollution in zones of bivalve mollusks at the Mediterranean coast of Egypt. A miniaturized MPN real-time PCR method for rapid quantification of total and enteropathogenic Vibrio parahaemolyticus in shellfish. Viviana Cachicas, ISP, Chile: Moustaf El-Shenawy, National Research Centre. Egypt Dominique Hervio-Heath & Michèle Gourmelon, IFREMER, France: Why has Risk Analysis failed us globally? POSTERS Dorothy – Jean Mc Coubrey BIOTOXINS Evaluation of the possible source of discrepancy in the results obtained after the application of MBA and LC-MS/MS for the analysis of lipophilic toxins Ben-Gigirey, European Union Reference Laboratory for Marine Biotoxins (EURLMB) A validated PP2A Method - OKATEST-Adapted for determination of lipophilic in combination with LC –MS/MS Elena Dominguez Perez ZEULAB S.L.España Biotoxins monitoring program in Brazil Mathias Schramm, Brazil Modelling the spatial and temporal dynamics of paralytic shellfish toxins at different scales: implications for research and management Marine toxins: new technologies and their impact in mussel farming. Alexandrium catenella cysts in Southern Chile: An overview of fifteen years of studies Miriam Seguel, Centro Regional de Análisis de Recursos y Medio Ambiente Universidad Austral de Chile Myrriam Seguel. Centro Regional de Análisis de Recursos y Medio Ambiente Universidad Austral de Chile. Miriam Seguel. Centro Regional de Análisis de Recursos y Medio Ambiente Universidad Austral de Chile National Program of surveillance and control of intoxications and Harmful algae blooms (Red tide) in Chile The first well documented event of Paralytic Shellfish Toxins (PST) in blue mussels (Mytilus edulis ) in Sweden Lorena Delgado ISP Chile Malin Persson, Sweden BACTERIA & VIRUS Michèle Gourmelon & Occurrence of bacterial and viral enteric pathogens and marine bacteria and Soizick Le Guyader, 1LSEMdiscrimination of faecal sources in shellfish-harvesting areas and their SG2M-RBE, IIFREMER, catchments in France Université de Caen, France: Characterization of Vibrio parahaemolyticus in bivalve molluscs from the coast of Buenos Aires, Argentina using pulsed-field gel electrophoresis (PFGE) Presence of Arcobacter spp. In bivalve molluscs from harvesting areas of the Central Adriatic Sea, Italy Norovirus prevalence in Dutch production area with Class A/B classification. Norovirus from environment samples associated to outbreaks in Chile 20102014 Veronica Jurquiza, INIDEP Argentina Francesca Leoni Sezione di Ancona Istituto Zooprofilattico Sperimentale dell´Umbria e delle Marche Italy Pol-Hofstad, Irene. Laboratory for Zoonoses and Environmental Microbiology RIVM. Netherland Monica Jara. Food Microbiology ISP Chile Detection and quantification of genotypes I and II of Norovirus in bivalve molluscs of the Region of Los Lagos in estival season 2015 Tulane virus: a surrogate to study norovirus behavior in oyster? Fernanda Carcamo Food Microbiology ISP –Chile Le Guyader, Soizick1., LSEM/SG2M IFREMER.2College of medecine University of Cincinnati.3UMR 6299 INSERM-CNRS. Presented by Michelle Gourmelon