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Supplemental Fig 1. Binding of human FH to pneumococcus is mediated by PspC. (A) D39
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and (B) D39 ΔPspC were employed in FH binding assays. Fluorescence intensity of bacteria
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(dotted line) and bacteria incubated with 10µg/ml of FH (filled black) are shown on the Y axis.
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(C) Levels of anti-PspC IgG and FH were measured in samples purified from the serum of 7
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subjects. The two-step purification process was able to purify anti-PspC specific IgG but FH from
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sera could not be entirely removed.
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Supplemental Fig 2. PspC epitope mapping using sera from mice immunized with
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recombinant PspC3 reveals the presence of antibodies to the FH binding site. Sera from
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mice immunized with the recombinant protein PspC3 was used to probe peptide arrays,
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covering the N-terminal region until the proline-rich region of PspC group 3. Sequences of the
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peptides recognized by murine sera are shown in black and the ones not recognized are shown
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in grey. Binding sites for FH and secretory Immunoglobulin A (sIgA) are represented in dashed
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black and continuous grey boxes, respectively.
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