SHU-HUA, YANG (楊曙華) by sharon, 2011-01-26 15:21, 人氣(223) ASSISTANT PROFESSOR TISSUE REGENERATION OF THE INTERVERTEBRAL DISC Degeneration of the intervertebral disc (IVD) and associated spinal disorders are leading causes of morbidity, resulting in substantial pain and increased health costs. Pathophysiologic evidence indicates that IVD degeneration originates from the nucleus pulposus (NP), where the proteoglycan content and type II collagen synthesis decrease, while denaturation of type II collagen and synthesis of type I collagen increase. As the NP loses its osmotic properties and becomes fibrotic, the IVD cannot transmit intervertebral forces optimally, and various degenerative processes subsequently occur. Before fusing the diseased segments as a salvage procedure for the end-stage pathology, ideal strategy should be applying different treatment to different stage of IVD degeneration. Regeneration of the NP tissues in the early stages of degeneration can theoretically retard or even reverse the degenerative process and possibly restore a healthy IVD. Our researches on IVD regeneration cover three key components of tissue engineering. Through collaboration with Graduate Institute of Biomedical Engineering, National Taiwan University, several biomaterials have been investigated for their effect on the IVD cells regarding enhancement of survival, proliferation and matrix synthesis. In order to completely fill the NP defect surrounded by the annulus fibrosis, injectable biomaterials are of special research interest in our recent researches. In addition to in-vitro studies, we are currently working on establishing a rat tail disc model for following in-vivo experiments. We also study the NP cells isolated from human tissue regarding their regeneration potential as well as optimal stimuli, including chemical and physical, to enhance the property of regenerative tissues. • Yang SH, Chen PQ, Chen YF, Lin FH. An in vitro study on regeneration of human nucleus pulposus by using gelatin/chondroitin-6-sulfate / hyaluronan tri-copolymer scaffold. Artificial Organs 2005, 29:806-14 • Yang SH, Lin CC, Hu MH, Shih TTF, Sun YH, Lin FH. Influence of age-related degeneration on regenerative potential of human nucleus pulposus cells. Journal of Orthopaedic Research 2010, 28:379-383.