DNA Review guide answers 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. DNA Reproduces S phase(interphase) Nucleotides Transcription Replication Base Translation Chargaff Rosalind Franklin Uracil Thymine Pyrimadine Dna: 2 strands, RNA 1 strand; DNA-T, RNA-U; DNA-Deoxyribose sugar, RNA-ribose sugar; DNA1 form, RNA-3 forms DNA Polymerase Watson and Crick Replication fork DNA Helicase Griffith mRNA tRNA Nitrogenous Germline Topoismerase Frameshift Non-sense Amino Acid Hydrogen Replication Fork RNA Polymerase Anticodon AUG- Methionine Methionine UAA, UAG, UGA mRNA Gene Chromosomes Genetic Mutation Worksheet Answers 1. A. Frameshift: Addition/Deletion of a base: shifts the sequence to the left or right changing all codons. b. Substitution: Mis-sense: changes one codon, changes the protein Non-sense: changes the codon to a stop Silent: changes the codon but codes for the same Amino Acid so no effect will be seen. c. Germline: carried on sex chromosomes passed on from generation to generation. 2. 3. 4. 5. 6. Substitution: Silent- changes the codon but codes for the same Amino Acid so it does not change the protein. Serine: UCU, UCC, UCG, UCA Tryptophan: UGG TEF ATC ATA TET HER AT: does not make sense it is a point deletion: frameshift 29 1- ser, lue, ala, ther, arg, seri 2- ser lue ala ther, seri, seri 3- ser, lue, ala, ther, arg, ser # 1 and #3 code for the same protein 7. A. CCC GAA GAA AAA- prol glut acid glut acid lysine b. point mutation- 5th base is changed from T to and A c. No, it would not be the same protein being made. DNA/RNA Protein Synthesis Review Guide Answers. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. Phosphate, sugar and base Twisted ladder; sides are phosphates and sugars and steps are the bases A-T, C-G TAGCTAAATTCCGGCGTAATGCGCAATCCTAG Break hydrogen bonds between DNA bases to unwind the 2 strands DNA polymerase attaches the correct nucleotides to make a new strand of DNA 2 similarities: They are both made of nucleotides, Both have ACG 4 Differences: DNA: 2 stranded RNA 1 stranded, DNA: Deoxyribose sugar RNA:Ribose sugar, DNA: 1 form RNA:3 forms DNA:T RNA:U mRNA: Carries the code for tRNA to get amino acids to make a protein tRNA: transfers amino acids from cytoplasm back to mRNA to make a protein rRNA: makes up ribosomes and enables ribosomes to attach to mRNA RNA polymerase attaches to DNA and breaks hydrogen bonds between DNA bases. Then it attaches correct nucleotides to make RNA. Occurs in the nucleus. Amino acids Ribosome reading mRNA 3 bases at a time called a codon. This codes for specific amino acids to make a protein. Occurs in the cytoplasm. 3 bases in mRNA that code for amino acids 3, 1 AUG CAU UUU GGA AGA ACC UCC GCA CCG CAG UAA Start, hist, phen, gly, arg, thre, ser, ala, prol, glut, stop