Journal of Biogeography
SUPPORTING INFORMATION
Historical biogeography of the widespread spider wasp tribe Aporini
(Hymenoptera: Pompilidae)
Juanita Rodriguez, James P. Pitts and Carol D. von Dohlen
Appendix S2 Additional information (Tables S1–S3) on the primers used for polymerase
chain reaction (PCR) amplification and sequencing of DNA from various spider wasp
species from the tribe Aporini, the partitioning scheme as determined by
PARTITIONFINDER, and the node age with 95% confidence intervals, in order to study the
historical biogeography of this group in a molecular phylogenetic context using
divergence-time estimation methods.
Table S1 The primers used for PCR amplification and sequencing.
Marker
EF1
LWRh
Primer name
Primer sequence (5′–3′)
Reference
F2for1
GGT TCC TTC AAA TAT GCT TGG G
Pilgrim et al. (2008)
F2rev1
A ATC AGC AGC ACC TTT AGG TGG
Danforth & Ji (1998)
PompOps1F
ATT CGA CAG ATA CAA CGT AAT CG
Pilgrim et al. (2008)
LwRhR
ATA TGG AGT CCA NGC CAT RAA
Mardulyn &
CCA
Cameron (1999)
GAG RGA GAT CGT CAT CAA GGC
This study
LwRhRApor
GAC C
Wg
LepWg1for
modLepWg2rev
28S
CF2
GAR TGY AAR TGY CAY GGY ATG
Brower & DeSalle
TCT GG
(1998)
ACT ICG CRC ACC ART GGA ATG TRC
Brower & DeSalle
A
(1998)
TGG TAA CTC CAT CTA AGG CTA
Pilgrim et al. (2008)
AAT A
D5–4625 R
CCC ACA GCG CCA GTT CTG CTT
(D5R)
ACC
Schulmeister (2003)
EF1, elongation factor-1  F2 copy; LWRh, long-wavelength rhodopsin; Wg, wingless;
28S, the D2–D3 regions of the 28S ribosomal RNA.
Table S2 The best partitioning scheme determined by PARTITIONFINDER and the loci
included in each.
Subset
1
2
Best model
SYM+I+G
K80+I
3
4
K80+G
GTR+I+G
Subset partitions
28S, 3rd codon position LWRh
1st codon position EF1, 2nd codon position EF1, 2nd
codon position LWRh
3rd codon position EF1
1st codon position LWRh, Wg
EF1, elongation factor-1  F2 copy; LWRh, long-wavelength rhodopsin; Wg, wingless;
28S, the D2–D3 regions of the 28S ribosomal RNA.
Table S3 The nodes recovered by BEAST analysis, their posterior probabilities (PP), their
ages (Ma), and 95% confidence intervals (CI) for the highest posterior density (HPD).
Node
PP
Age
HPD 95% CI
Taxonomic group
A
1.00
22.66
17.40, 28.83
Aporini
B
0.95
20.91
15.89, 26.58
C
1.00
18.22
13.81, 23.52
D
0.99
15.57
11.14, 20.64
E
1.00
11.19
7.11, 15.47
F
1.00
0.66
0.075, 1.80
G
0.95
9.64
5.82, 13.65
H
1.00
1.30
0.29, 3.04
I
1.00
5.35
2.85, 8.58
J
1.00
0.31
0.010, 1.01
K
0.99
12.31
7.83, 17.57
L
1.00
7.21
4.43, 10.85
M
1.00
2.53
0.75, 5.29
N
0.99
4.66
2.14, 8.02
O
1.00
15.81
11.79, 20.74
P
1.00
7.61
4.43, 11.34
Q
1.00
2.601
1.03, 4.85
R
1.00
1.72
0.59, 3.36
S
1.00
4.86
2.43, 8.00
T
1.00
11.49
8.17, 15.61
U
0.99
9.55
6.60, 13.06
V
1.00
6.13
3.12, 9.35
W
1.00
0.43
0.053, 1.15
X
0.67
8.75
5.92, 12.12
Y
1.00
2.89
1.37, 4.92
Z
0.98
18.14
13.28, 23.71
AA
1.00
12.97
8.76, 18.02
BB
1.00
6.36
4.17, 9.18
CC
0.97
4.68
2.34, 7.43
DD
1.00
0.82
0.065, 2.29
EE
0.97
5.28
3.33, 7.65
FF
1.00
3.40
1.81, 5.38
Aporus
Drepanaporus
Euplaniceps
Psorthaspis
Node
PP
Age
HPD 95% CI
GG
0.64
4.37
2.51, 6.63
HH
1.00
1.40
0.44, 2.79
II
1.00
3.40
1.81, 5.38
JJ
0.99
1.75
0.75, 3.09
KK
1.00
8.86
5.92, 12.55
LL
0.92
7.21
3.97, 11.02
MM
1.00
3.01
5.46  104, 0.0028
NN
1.00
0.91
0.22, 2.06
OO
1.00
1.18
0.29, 2.66
PP
1.00
9.19
5.03, 14.63
QQ
0.99
1.28
0.023, 4.06
Taxonomic group
Allaporus
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