Wenxu, CV
CURRICULUM VITA
Wenxu Zhou
EDUCATION
2002
Ph.D. (Biochemistry Division), Dept. of Chemistry and Biochemistry, Texas
Tech University
M.S. (Biochemistry Division), Dept. of Chemistry and Biochemistry, Texas Tech
University
B.S. (in pharmacy) Beijing Medical University, P.R. China.
1999
1989
PROFESSIONAL EXPERIENCE
2007-present Research Assistant Professor, UWA Centre for Energy, ACR CoE Plant Energy
Biology. The University of Western Australia
2006-2007
Associate Manager of W. M. Keck Metabolomics Research Laboratory, Iowa
State University.
2002-2006
Research Associate and Laboratory Manger, Biochemistry, Department of
Chemistry and Biochemistry, Texas Tech University
PUBLICATIONS
.1.
2.
3.
4.
5.
6.
Shingaki-Wells RN, Huang SB, Taylor NL, Carroll AJ, Zhou WX, Millar AH:
Differential Molecular Responses of Rice and Wheat Coleoptiles to Anoxia Reveal
Novel Metabolic Adaptations in Amino Acid Metabolism for Tissue Tolerance. Plant
Physiol 2011, 156(4):1706-1724.
Schmidt MA, Barbazuk WB, Sandford M, May G, Song ZH, Zhou WX, Nikolau BJ,
Herman EM: Silencing of Soybean Seed Storage Proteins Results in a Rebalanced
Protein Composition Preserving Seed Protein Content without Major Collateral
Changes in the Metabolome and Transcriptome. Plant Physiol 2011, 156(1):330-345.
Pracharoenwattana I, Zhou WX, Smith SM: Fatty acid beta-oxidation in germinating
Arabidopsis seeds is supported by peroxisomal hydroxypyruvate reductase when
malate dehydrogenase is absent. Plant MolBiol 2010, 72(1-2):101-109.
Pracharoenwattana I, Zhou WX, Keech O, Francisco PB, Udomchalothorn T, Tschoep H,
Stitt M, Gibon Y, Smith SM: Arabidopsis has a cytosolic fumarase required for the
massive allocation of photosynthate into fumaric acid and for rapid plant growth on
high nitrogen. Plant J 2010, 62(5):785-795.
Che P, Bussell JD, Zhou WX, Estavillo GM, Pogson BJ, Smith SM: Signaling from the
Endoplasmic Reticulum Activates Brassinosteroid Signaling and Promotes
Acclimation to Stress in Arabidopsis. Sci Signal 2010, 3(141).
Wiszniewski AAG, Zhou WX, Smith SM, Bussell JD: Identification of two
Arabidopsis genes encoding a peroxisomal oxidoreductase-like protein and an acylCoA synthetase-like protein that are required for responses to pro-auxins. Plant
MolBiol 2009, 69(5):503-515.
1
Wenxu, CV
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
Timmins M, Zhou WX, Rupprecht J, Lim L, Thomas-Hall SR, Doebbe A, Kruse O,
Hankamer B, Marx UC, Smith SM et al: The metabolome of Chlamydomonas
reinhardtii following induction of anaerobic H(2) production by sulfur depletion
(vol 284, pg 23415, 2009). J Biol Chem 2009, 284(51):35996-35996.
Nes WD, Zhou WX, Ganapathy K, Liu J, Vatsyayan R, Chamala S, Hernandez K,
Miranda M: Sterol 24-C-methyltransferase: An enzymatic target for the disruption
of ergosterol biosynthesis and homeostasis in Cryptococcus neoformans. Arch
Biochem Biophys 2009, 481(2):210-218.
Li J, Francisco P, Zhou WX, Edner C, Steup M, Ritte G, Bond CS, Smith SM:
Catalytically-inactive beta-amylase BAM4 required for starch breakdown in
Arabidopsis leaves is a starch-binding-protein. Arch Biochem Biophys 2009, 489(12):92-98.
Zhou WX, Nguyen HT, Nes WD: Plant Sterol Methyltransferases: Phytosterolomic
Analysis, Enzymology, and Bioengineering Strategies. In: Advances in Plant
Biochemistry and Molecular Biology. Edited by Hans J. Bohnert HN, Norman GL, vol.
Volume 1: Pergamon; 2008: 241-281.
Cousins AB, Pracharoenwattana I, Zhou WX, Smith SM, Badger MR: Peroxisomal
malate dehydrogenase is not essential for photorespiration in Arabidopsis but its
absence causes an increase in the stoichiometry of photorespiratory CO2 release.
Plant Physiol 2008, 148(2):786-795.
Zhou W, Cross GAM, Nes WD: Cholesterol import fails to prevent catalyst-based
inhibition of ergosterol synthesis and cell proliferation of Trypanosoma brucei. J
Lipid Res 2007, 48(3):665-673.
Zhou WX, Lepesheva GI, Waterman MR, Nes WD: Mechanistic analysis of a multiple
product sterol methyltransferase implicated in ergosterol biosynthesis in
Trypanosoma brucei. J Biol Chem 2006, 281(10):6290-6296.
Nes WD, Sinha A, Jayasimha P, Zhou WX, Song ZH, Dennis AL: Probing the sterol
binding site of soybean sterol methyltransferase by site-directed mutagenesis:
Functional analysis of conserved aromatic amino acids in Region 1. Arch Biochem
Biophys 2006, 448(1-2):23-30.
Lepesheva GI, Zaitseva NG, Nes WD, Zhou WX, Arase M, Liu J, Hill GC, Waterman
MR: Cyp51 from Trypanosoma cruzi - A phyla-specific residue in the B ' helix
defines substrate preferences of sterol 14 alpha-demethylase. J Biol Chem 2006,
281(6):3577-3585.
Zhou WX, Song ZH, Kanagasabai R, Liu JL, Jayasimha P, Sinha A, Veeramachanemi P,
Miller MB, Nes WD: Mechanism-based enzyme inactivators of phytosterol
biosynthesis. Molecules 2004, 9(4):185-203.
Zhou WX, Song ZH, Kanagasabai R, Liu JL, Jayasimha P, Sinha A, Veeramachanemi P,
Miller MB, Nes WD: Mechanism-based enzyme inactivators of phytosterol
biosynthesis. Molecules 2004, 9(4):185-203.
Song ZH, Zhou WX, Liu JL, Nes WD: Mechanism-based active site modification of
the soybean sterol methyltransferase by 26,27-dehydrocycloartenol. Bioorganic &
Medicinal Chemistry Letters 2004, 14(1):33-36.
Nes WD, Jayasimha P, Zhou WX, Kanagasabai R, Jin CX, Jaradat TT, Shaw RW,
Bujnicki JM: Sterol methyltransferase: Functional analysis of highly conserved
residues by site-directed mutagenesis. Biochemistry 2004, 43(2):569-576.
2
Wenxu, CV
20.
21.
22.
23.
24.
25.
26.
27.
28.
29.
30.
Lepesheva GI, Nes WD, Zhou WX, Hill GC, Waterman MR: CYP51 from
Trypanosoma brucei is obtusifoliol-specific. Biochemistry 2004, 43(33):10789-10799.
Lepesheva G, Nes D, Zhou WX, George H, Waterman M: Substrate preference of
CYP51 from Trypanosoma brucei may reflect specificity of sterol biosynthesis in
Kinetoplastida. Faseb J 2004, 18(8):C178-C178.
Kanagasabai R, Zhou WX, Liu JL, Nguyen TTM, Veeramachaneni P, Nes WD:
Disruption of ergosterol biosynthesis, growth, and the morphological transition in
Candida albicans by sterol methyltransferase inhibitors containing sulfur at C-25 in
the sterol side chain. Lipids 2004, 39(8):737-746.
Zhou WX, Nes WD: Sterol methyltransferase2: purification, properties, and
inhibition. Arch Biochem Biophys 2003, 420(1):18-34.
Nes WD, Song ZH, Dennis AL, Zhou WX, Nam J, Miller MB: Biosynthesis of
phytosterols - Kinetic mechanism for the enzymatic C-methylation of sterols. J Biol
Chem 2003, 278(36):34505-34516.
Zhou WX, Nguyen TTM, Collins MS, Cushion MT, Nes WD: Evidence for multiple
sterol methyl transferase pathways in Pneumocystis carinii. Lipids 2002,
37(12):1177-1186.
Nes WD, Zhou WX, Dennis AL, Li HX, Jia ZH, Keith RA, Piser TM, Furlong ST:
Purification, characterization and catalytic properties of human sterol 8-isomerase.
Biochemical Journal 2002, 367:587-599.
Zhou WX, Nes WD: Stereochemistry of hydrogen introduction at C-25 in ergosterol
synthesized by the mevalonate-independent pathway. Tetrahedron Letters 2000,
41(16):2791-2795.
Diener AC, Li HX, Zhou WX, Whoriskey WJ, Nes WD, Fink GR: STEROL
METHYLTRANSFERASE 1 controls the level of cholesterol in plants. Plant Cell
2000, 12(6):853-870.
Nes WD, McCourt BS, Zhou WX, Ma JZ, Marshall JA, Peek LA, Brennan M:
Overexpression, purification, and stereochemical studies of the recombinant (S)adenosyl-L-methionine: Delta(24(25))- to Delta(24(28))-sterol methyl transferase
enzyme from Saccharomyces cerevisiae. Arch Biochem Biophys 1998, 353(2):297-311.
Tong Y, McCourt BS, Guo DA, Mangla AT, Zhou WX, Jenkins MD, Zhou W, Lopez M,
Nes WD: Stereochemical features of C-methylations on the path to Delta 24(28)methylene and Delta(24(28))-ethylidene sterols: Studies on the recombinant
phytosterol methyl transferase from Arabidopsis thaliana. Tetrahedron Letters 1997,
38(35):6115-6118.
3