OIE Reference Laboratory Reports
Activities in 2012
Name of disease (or topic) for which you
are a designated OIE Reference Laboratory:
Transmissible gastroenteritis virus
Address of laboratory
Food Animal Health Research Program, Ohio
Agricultural Research and Development Center
The Ohio State University
1680 Madison Avenue, Wooster, OH 44691
Tel.:
330-263-3744
Fax:
330-263-3677
e-mail address:
saif.2@osu.edu
website:
http://www.oardc.ohio-state.edu/lsaiflab/
Name (including Title) of Head of
Laboratory (Responsible Official):
Linda J. Saif, PhD
Distinguished University Professor
Name (including Title and Position) of OIE
Reference Expert:
Linda J. Saif, PhD
Distinguished University Professor
Date of submission to the OIE
1-3-12
Instructions
This form should be used by an OIE Reference Laboratory to report activities that took place from January
through December of the past year (2012), unless otherwise stated, and must be submitted by the end of January
every year.
Only those activities that concern the disease (or topic) for which the laboratory is recognised by the OIE should
be mentioned. The questionnaire structure follows the Terms of Reference (ToRs) for OIE Reference Laboratories,
available at:
http://www.oie.int/en/our-scientific-expertise/reference-laboratories/introduction/
Each ToR (blue italicised text) has been placed as a heading covering the group of questions related to it.
Please note the red italicised text is given as guidance and should be deleted from your report and substitute with
your data. Examples are based on past Annual Reports or have been invented.
The questionnaire represents a means of gathering information on activities carried out by OIE Reference
Laboratories and making it available to OIE Member Countries and to the OIE Reference Laboratory network.
This annual report will remain available for consultation on the OIE web site:
(http://www.oie.int/en/our-scientific-expertise/reference-laboratories/annual-reports/):
Annual reports of OIE Reference Centres, 2012
1
OIE RL for « Transmissible gastroenteritis » – « Linda J. Saif » – « United States »
ToR: To use, promote and disseminate diagnostic methods validated according to OIE
Standards
Test recommended by the OIE
Total number of test performed last year
Indirect diagnostic tests
Nationally
Internationally
Antibody-ELISA
15 tests
0 tests
SN Plaque reduction)
15
0
Antigen ELISA
20
0
Direct diagnostic tests
Nationally
Internationally
Cell Culture Immunofluorescence (ST cells)
20
0
RT-PCR and nested PCR
50
5
ToR: To develop reference material in accordance with OIE requirements, and implement and
promote the application of OIE Standards.
To store and distribute to national laboratories biological reference products and any
other reagents used in the diagnosis and control of the designated pathogens or disease.
2.
Did your laboratory produce or store imported standard reference reagents officially recognised by the
OIE or other international bodies?
XYes
3.
No
Did your laboratory supply standard reference reagents to OIE Member Countries?
X Yes
Related
diagnostic
test
Control positive TGE
virus and viral RNA
RT-PCR
Control positive TGE
viral RNA and 4
primers for RT-PCR
RT-PCR
Type of reagent
available
2
No
Produced/
stored
Amount supplied
nationally
(ml, mg)
Amount supplied
internationally
(ml, mg)
Produced
various (positive)
300ul TGEV
50ul RNA
Produced
various (positive)
100ul TGEV RNA
60 ul of each of 4
primers
Name of
recipient OIE
Member
Countries and
of institutions
SENESA DILAB
BA, Argentina
National
Reference Lab
SENESA
Lima,Peru
National
Reference
Laboratory
Annual reports of OIE Reference Centres, 2012
OIE RL for « Transmissible gastroenteritis » – « Linda J. Saif » – « United States »
4.
Type of reagent
available
Related
diagnostic
test
Control positive
monoclonal
antibody to TGEV
Immunofluo
rescence,
ELISA
Produced/
stored
Amount supplied
nationally
(ml, mg)
Amount supplied
internationally
(ml, mg)
Produced
200ul
0
No
Did your laboratory produce vaccines?
Yes
6.
Iowa State
University
Diagnostic
Laboratory
Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference
reagents?
X Yes
5.
Name of
recipient OIE
Member
Countries and
of institutions
X No
Did your laboratory supply vaccines to OIE Member Countries?
Yes
Vaccine name
XNo
Amount supplied nationally (ml,
mg) (including for own use)
Amount supplied to other
countries (ml, mg)
Name of recipient
OIE Member
Countries
ToR: To develop, standardise and validate, according to OIE Standards, new procedures for
diagnosis and control of the designated pathogens or diseases
7.
Did your laboratory develop new diagnostic methods validated according to OIE Standards for the
designated pathogen or disease?
XYes
8.
No
Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or
disease?
Yes
Name of the new test or diagnostic
method or vaccine developed
TGEV/PRCV monoclonal antibody
differential ELISA
Annual reports of OIE Reference Laboratories, 2012
X No
Description and References (Publication, website, etc.)
. Simkins, R. A., P. A. Weilnau, J. Van Cott, T. Brim
and L. J. Saif. 1993. Competition ELISA, using
monoclonal antibodies to the TGEV S protein, for
serologic differentiation of pigs infected with
TGEV or porcine respiratory coronavirus. Am. J.
3
OIE RL for « Transmissible gastroenteritis » – « Linda J. Saif » – « United States »
Vet. Res. 54:254-259.
Immunocytochemistry
Shoup, D. I., D. E. Swayne, D. J. Jackwood and L. J.
Saif. 1996. Immunocytochemistry of transmissible
gastroenteritis virus antigens in fixed paraffinembedded tissues. J. Vet. Diag. Invest. 8:161167.
TGEV/PRCV S-protein differential ELISA
Sestak, K., Z. Zhou, D.I. Shoup and L.J. Saif. 1999.
Evaluation of the baculovirus-expressed S
glycoprotein of transmissible gastroenteritis virus
(TGEV) as antigen in a competition ELISA to
differentiate porcine respiratory coronavirus from
TGEV antibodies in pigs. J. Vet. Diagn. Invest.
11:205-214.
TGEV/PRCV differential RT-PCR
Kim, L., K.O. Chang, K. Sestak, A. Parwani and L.J.
Saif. 2000.
Development of a reverse
transcription-nested PCR assay for differential
diagnosis of transmissible gastroenteritis virus
(TGEV) and porcine respiratory coronavirus
(PRCV) from feces and nasal swabs of infected
pigs. J. Vet. Diagn. Invest. 12:385-388.
N-protein-specific ELISA for TGEV vs SARS
discrimination
Vlasova, A., X. Zhang, M. Hasoksuz, N. Hadya and
L.J. Saif. 2008. The two-way antigenic crossreactivity between SARS-CoV and group 1
animal CoVs is mediated through an antigenic
site located in the N-terminal region of the
SARS-CoV nucleoprotein. J. Virol. 81:1336513377.
ToR: To provide diagnostic testing facilities, and, where appropriate, scientific and technical
advice on disease control measures to OIE Member Countries
9.
Did your laboratory carry out diagnostic testing for other OIE Member Countries?
XYes
10.
No
Name of OIE
Member Country
seeking assistance
Date
(dd/mm)
No. samples received for
provision of diagnostic support
(i.e. from surveillance campaign)
No. samples received for
provision of confirmatory
diagnoses
Argentina
Jan
4
4
Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member
Country?
XYes
Name of the OIE Member Country
4
No
Purpose
How the advice was
Annual reports of OIE Reference Centres, 2012
OIE RL for « Transmissible gastroenteritis » – « Linda J. Saif » – « United States »
receiving a technical consultancy
Argentina
provided
PT-PCR for TGEV/coronavirus diagnosis
remote
PT-PCR for TGEV/coronavirus diagnosis
remote
PT-PCR for TGEV/PEDV/coronavirus
diagnosis and vaccines
In loco
PT-PCR for TGEV/PEDV/coronavirus
diagnosis and vaccines
in loco
SENESA DILAB BA, Argentina National
Reference Laboratory
Peru
SENESA, Lima, Peru National Reference
Laboratory
China
State Key Laboratory of Agricultural
Microbiology, Huazhong
Agricultural University, Wuhan,
China.
China
Harbin Veterinary Research
Institute, Chinese Academy of
Agricultural Sciences , Harbin,
China.
ToR: To carry out and/or coordinate scientific and technical studies in collaboration with other
laboratories, centres or organisations
11.
Did your laboratory participate in international scientific studies in collaboration with OIE Member
Countries other than the own?
XYes
No
Title of the study
Duration
Purpose of the study
TGEV vaccine
Ongoing
Develop and test
TGEV vaccines
Partners
(Institutions)
OIE Member Countries
involved other than
your country
Dr Luis Enjuanes,
Spain
CENTRO
NACIONAL DE
BIOTECNOLOGIA
(CNB)
SPANISH
NATIONAL
RESEARCH
COUNCil
Campus
Universidad
Autonoma
Madrid, Spain
Annual reports of OIE Reference Laboratories, 2012
5
OIE RL for « Transmissible gastroenteritis » – « Linda J. Saif » – « United States »
TGEV/PEDV
vaccines and
Diagnostics
Ongoing
Develop and test
TGEV and PEDV
vaccines and
diagnostics
Dr Huanchun
Chen, Director of
the State Key
Laboratory of
Agricultural
Microbiology,
Huazhong
Agricultural
University, Wuhan,
China.
China
TGEV/PEDV
vaccines and
Diagnostics
Ongoing
Develop and test
TGEV and PEDV
vaccines and
diagnostics
Dr Li Feng, Harbin
Veterinary
Research Institute,
Chinese Academy
of Agricultural
Sciences , Harbin,
China.
China
Animal
coronavirus
reference strains
and antisera
Ongoing
Provide animal
coronavirus
reference reagents
(deposited at ATTC)
and screen for animal
coronaviruses or
antibodies
World Health
Organization
Geneva, Switzerland
ToR: To collect, process, analyse, publish and disseminate epizootiological data relevant to the
designated pathogens or diseases
12.
Did your Laboratory collect epizootiological data relevant to international disease control?
X Yes
13.
No
Did your laboratory disseminate epizootiological data that had been processed and analysed?
X Yes
14.
No
What method of dissemination of information is most often used by your laboratory?
(Indicate in the appropriate box the number by category)
a)
Articles published in peer-reviewed journals: ................. 2
b)
International conferences: .............................................. 2
c)
National conferences: ...................................................... 2
d)
Other: ............................................................................... book chapters, internet
ToR: To provide scientific and technical training for personnel from OIE Member Countries
To recommend the prescribed and alternative tests or vaccines as OIE Standards
6
Annual reports of OIE Reference Centres, 2012
OIE RL for « Transmissible gastroenteritis » – « Linda J. Saif » – « United States »
15.
Did your laboratory provide scientific and technical training to laboratory personnel from other OIE
Member Countries?
X Yes
No
If the answer is yes, please provide the total number of trained persons for each of the following
categories:
a)
Technical visits: ................................................................
b)
Seminars: .........................................................................
c)
Hands-on training courses: .............................................. 2
d)
Internships (>1 month): ................................................... 2
Type of technical training
provided (a, b, c or d)
Country of origin of the expert(s)
provided with training
No. participants from the
corresponding country
C,d
Ethiopia
1
C,d
Kenya
1
ToR: To maintain a system of quality assurance, biosafety and biosecurity relevant for the
pathogen and the disease concerned
16.
Does your laboratory have a Quality Management System certified according to an International
Standard?
Yes
17.
X No
Is your laboratory accredited by an international accreditation body?
Yes
XNo
18. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease
concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 or
Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1)
X Yes
No
ToR: To organise and participate in scientific meetings on behalf of the OIE
19.
Did your laboratory organise scientific meetings on behalf of the OIE?
Yes
20.
X No
Did your laboratory participate in scientific meetings on behalf of the OIE?
Yes
Annual reports of OIE Reference Laboratories, 2012
X No
7
OIE RL for « Transmissible gastroenteritis » – « Linda J. Saif » – « United States »
ToR: To establish and maintain a network with other OIE Reference Laboratories designated
for the same pathogen or disease and organise regular inter-laboratory proficiency
testing to ensure comparability of results
21.
Did your laboratory exchange information with other OIE Reference Laboratories designated for the
same pathogen or disease? Our lab is only reference lab for TGEV
Yes
22.
XNo
Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for
the same pathogen or disease by organising or participating in proficiency tests?
Yes
23.
XNo
Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on
scientific research projects for the diagnosis or control of the pathogen of interest?
Yes
X No
ToR: To organise inter-laboratory proficiency testing with laboratories other than OIE
Reference Laboratories for the same pathogens and diseases to ensure equivalence of
results.
24.
Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories
other than OIE Reference Laboratories for the same disease?
Yes
X No
ToR: To place expert consultants at the disposal of the OIE
25.
Did your laboratory place expert consultants at the disposal of the OIE?
XYes
No
Kind of consultancy
Location
Subject (facultative )
Revisions for OIE Manual of Diagnostic
Tests and Vaccines for Terrestial Animals
Paris
Author revisions to Chapter 2.6.4
OIE Terrestrial Manual
Publications 2011-12
Vlasova, A., R. Halpin, S. Wang, E. Ghedin, D.J. Spiro, and L.J. Saif. 2011. Molecular characterization of a new
alphacoronavirus species associated with mink epizootic catarrhal gastroenteritis (ECG). J. of Gen. Virol.
92:1369-79
8
Annual reports of OIE Reference Centres, 2012
OIE RL for « Transmissible gastroenteritis » – « Linda J. Saif » – « United States »
Mullis, L., L.J. Saif, Y. Zhang, X. Zhang, and M.S.P. Azevedo. 2012. Stability of bovine coroanvirus on lettuce
surfaces under household refrigeration conditions. Food Microbiology 30:180-186.
Saif, L.J., M.P. Pensaert, K. Sestak, S.G. Yeo, K. Jung. 2012. Coronaviruses. In Diseases of Swine (J.J. Zimmerman, et
al. Eds.), 10th Edition, Wiley-Blackwell, Ames, Iowa. pp 501-524.
Vlasova, A.N. and L.J. Saif. 2012. Biological aspects of the interspecies transmission of selected coronaviruses. In
“Viral Infections and Climate Change”, (S. Singh, Ed) John Wiley & Sons/Wiley Blackwell Press (in press).
Annual reports of OIE Reference Laboratories, 2012
9